• Korean Journal of Food Science and Technology
• /
• v.26 no.5
• /
• pp.633-637
• /
• 1994

A bacterial strain KSM-35 producing maltotetraose forming amylase was isolated from compost and identified as Streptomyces based on its morphological, cultural, and physiological characteristics. The amylase from Streptomyces sp. KSM-35 culture filtrate was purified by ammonium sulfate precipitation, followed by the liquid chromatographic procedures using DEAE-Toyo pearl and sephadex G-100 with 27.1% activity recovery. The molecular weight of the enzyme was estimated to be 50,000 and the isoelectric point 4.3. The main product by the amylase from soluble starch was maltotetraose which accounted for 56% of all the oligosaccharides detected after 26 hrs of reaction. Maltose (20%o) and maltotriose (16%) were the next important byproducts while glucose and maltopentaose were detected as traces.

• Journal of Soil and Groundwater Environment
• /
• v.13 no.3
• /
• pp.59-66
• /
• 2008

A biological study was conducted to evaluate the enhancement of landfarming of soil contaminated with petroleum hydrocarbon (TPH) applying organic composite nutrients and a chemical oxidation during bioremediation. The target value of soil TPH after treatment was 500 mg/kg TPH. Addition of an organic compost and liquid swine manure for the removal of soil THP showed higher efficiency as 84.4% and 92.2% respectively than inorganic nutrients of 80.2%. In addition to the removal of non-biodegradable portion of residual hydrocarbons in soil, a chemical oxidation was applied during tailing period of the biological remediation, which showed high remediation efficiency as 98.1% compared with single bioremediation efficiency of 84.7%.

• Korean Journal of Soil Science and Fertilizer
• /
• v.39 no.6
• /
• pp.386-391
• /
• 2006

This study was conducted to evaluate the indole acetic acid (IAA) formation in soils as a biological indicator for the health of rice paddy soils with control, nitrogen sole, chemical fertilizer (NPK), and chemical fertilizer plus compost (CNPK) plots. There was a positive relationship between colorimetric method and high performance liquid chromatography for IAA in soils determined, and the values were similar between two methods, as $0.83{\sim}1.23{\mu}g\;IAA\;g^{-1}h^{-1}$ in colorimetric method, $0.80{\sim}1.29{\mu}g\;IAA\;g^{-1}h^{-1}$ in HPLC method. Numbers of dehydrogenase-producing bacteria and the IAA production in soils were high in NPK and CNPK plots comparing with control and nitrogen sole plots. Also there was high correlation between numbers of dehydrogenase-producing bacteria and IAA production in soils.

• Journal of the Korea Organic Resources Recycling Association
• /
• v.21 no.4
• /
• pp.72-81
• /
• 2013

The objective of this study was to verify whether SCB(Slurry Composting & Bio-filtration) system can be applied for the treatment of anaerobic digestion(AD) wastewater and also, to identify the most effective set among three filtration compost beds tested. Results can be summarized as these; (a) When AD wastewater was sprayed on the top of beds which were mainly composed of sawdust and/or other media and, subsequently, filtrates collected and analyzed, there were large drop in the values of Electric Conductivity(EC), Total Suspended Solid(TSS), Biochemical Oxygen Demand(BOD), and Chemical Oxygen Demand(COD). In contrast, Total Nitrgen(T-N) and Total Phosphorus(T-P) were progressively elevated. We consider these changes as positive if the filtrate are to be utilized as liquid fertilizer. (b) When three sets of filtration beds (T1, T2, T3) were compared for their effectiveness, no significant difference was found among them. These indicate that expensive sawdust can be replaced in part with cheaper media such as woodchip, rice husks, or others. (c) At early stage of operation (within 20 days), BOD in filtrates were maintained at high level probably due to the lack of microbial activity. During the same stage, T-N, T-P was at low level but, were elevated to higher levels thereafter. These data, when combined, indicate that the filtration system needs at least a couple of weeks for the optimized microbial functioning. (d) The temperatures of the experimental beds were progressively dropped as the experiment continued through the fall season, although filtration effectiveness was not noticeably influenced.

• Journal of The Korean Society of Grassland and Forage Science
• /
• v.28 no.2
• /
• pp.139-154
• /
• 2008

The nearly 75% of animal feed materials used for livestock production are imported every year in Korea. Most of imported feed ingredients are concentrated feeds such as com, wheat, soybean, soybean meal, etc. and they are used as the source materials for the production of assorted feed. The imported concentrated feeds are high in nitrogen and phosphorus. Therefore, the consistent import of feed ingredients may cause an increase of nutrient deposit in our agricultural ecosystem. In the current review, it was discussed with the situation of the feed importation and its nutritional composition to evaluate the nutrient load by the imported feeds onto agricultural ecosystem. The nutrient load caused by imported feeds in agricultural environment was compared with the nutrient demand for crop production. The amounts of N, $P_2O_5\;and\;K_2O$ introduced by the imported fteds in Korea were 371, 140 and 143 Ktons. And, the N, $P_2O_5\;and\;K_2O$ loads excreted from imported feeds in livestock were 148, 84 and 86 Ktons of N, $P_2O_5\;and\;K_2O$ and These nutrient loads by the imported feeds are at the percentage of N 52%, $P_2O_5$ 52% and $K_2O$ 42% in the comparison of total nutrient amounts excreted from livestock animals in Korea. The 82.3% of nutrients excreted from livestock was recycled to crop land as compost and liquid fertilizer, and the others were discharged to river after water treatment processing or disposed to ocean. Also, passing through the recycling process far the production of compost and liquid fertilizer, the amount of nutrients was reduced by the ammonia vaporization of livestock feces and urine. Accordingly, N 81, $P_2O_5$ 74 and $K_2O$ 76 Ktons in the nutrients excreted from livestock were estimated to be utilized in the crop land. Consequently, it was estimated that 44, 48 and 69 Ktons of N, $P_2O_5\;and\;K_2O$ were taken up with crops in the consideration of the ratio of mineralization, and the amounts of leached or deposited N, $P_2O_5\;and\;K_2O$ in crop land were estimated to be 37, 27 and 7 Ktons, respectively. It is estimated that 12%, 34% and 48% of N, $P_2O_5\;and\;K_2O$ introduced by the imported feeds were used by crops, and 10%, 34% and 5% of N, $P_2O_5\;and\;K_2O$ were leached or deposited in agricultural ecosystem. Therefore, considering the leached and deposited amounts of N, $P_2O_5\;and\;K_2O$ originated from the imported feed ingredients, the consistent import of feeds may gradually increase the nutrient load onto agricultural ecosystem.

• Korean Journal of Soil Science and Fertilizer
• /
• v.44 no.3
• /
• pp.353-360
• /
• 2011

In order to develop the application method of pig compost (PC) and liquid manure (LM) for whole crop barley cultivation, experiments were conducted at Munpo series (coarse loamy, mixed, nonacid, mesic family of Typic Fluvaquents) soil in Gyehwa-reclaimed land, six plots, a LM applied rate as N% ; non-application, chemical fertilizer (CF)100, 100, 50+50, 50+CF50 and (PC30+LM40)+LM50 as basal and additional fertilizer. $NO_3^-$-N content in soil was decreased as along with the growth of plant, highest in LM100% as basal fertilization at early growth stage and highest in (PC30%+LM40%)+LM40% and CF100% at last growth stage. Amount of $NO_3^-$-N and $NH_4^+$-N in soil was high in (PC30%+LM40%)+LM40% and CF100% of top soil but in subsoil significant difference was little in all treatment. Amount of OM, $A_V.P_2O_5$, T-N, exchangeable Ca and Na in soil was higher (PC30%+LM40%)+LM40% than non-application after harvest. Amount of nutrient uptake in plant was higher in CF100% and split application of LM than LM 100% application. Nitrogen utilization rate was in the order of CF100% >LM50%+LM50%=LM50%+CF50%>(PC30%+LM40%)+LM40% >LM100%. The yield of whole crop barley in (PC30%+LM40%)+LM40% and CF100% was 3.2 times more than in non-application ($309kg\;10a^{-1}$). Feed values such as crude protein and TDN was increased 1.0% ~ 1.4% in LM as split application than basal 100% treatment. Accordingly, in order to increase yield of a whole crop barley with application PC+LM in reclaimed land treat split application rather than to treat LM 100% into the land.

• Journal of Applied Biological Chemistry
• /
• v.64 no.4
• /
• pp.439-446
• /
• 2021

A field-scale study was conducted to evaluate the effect of organic matter amendments on the solubility of arsenic (As) in paddy soil and uptake by rice. Six organic matter (rice bran, rice straw, pig/cattle/fowls manure compost and swine liquid manure) were added to two polluted soils with high As (53 mg kg^-1) and low As concentration (28 mg kg^-1), and changes in soil solution constituents was monitored. The mean As concentrations in soil solution from the high As soil with rice bran, pig manure compost and swine liquid manure addition were significantly higher (0.61-1.15 mg L^-1) than that of the control (0.42-0.66 mg L^-1). Regression between As and Fe in soil solution indicated that As was attributable to reductive dissolution of Fe (hydr)oxides and it was driven by organic matter addition. Mean As concentrations in brown rice from the high As soil were 0.35-0.46 mg kg^-1, above the maximum safety level of inorganic As (0.35 mg kg^-1), and tended to be higher in organic matter amended soils than that of the control. The significant correlation between grain As and soil solution As was not observed and it was probably attributable to As tolerance of rice causing the reduction of As uptake and/or translocation to grain. However, considering the significant As release in soil solution from the high As soil and the tendency of grain As elevation after organic matter addition, it is needed to be cautious for food safety when amending organic matter to paddy soil with high As concentration.

• Journal of the Korea Organic Resources Recycling Association
• /
• v.13 no.1
• /
• pp.61-70
• /
• 2005

In order to reduce salt(as NaCl) contents in food waste and to improve the quality of discharged wastewater produced during the recycling process of food waste for the purpose of compost and feed stuff, a salt reduction process by added water into food waste was developed. The pilot plant with a rotary type salt reduction equipment to manage continuously 0.5 ton food waste per hour was constructed and the efficiency was tested. The amount of added water was calculated by the water content and the efficiency of dewatering process of food waste. Approximately 0.8 liter water per a kilogram of food waste was injected into the reactor in which food waste was pouring simultaneously, then diluted/mixed in a rotary reactor. About 1.1 liter of leachate including added water was generated, but the leachate contained a very high content of organic particles, so most particles were recovered by two step solid-liquid separation process. The first step was a gravitational filtering process using screens with a pore diameter of 1mm, and the second separation process was centrifugal process. Organic quality of food waste which had been desalted was maintained by inputting the entirely recovered organic particles. The efficiency of salt reduction of food waste was estimated by measuring a chloride anion by titration and salinity by a probe. The results by the two different measuring methods were always over 50%, and the quality of final wastewater was improved up to $200mg/{\ell}$ as TS(total solid) by an additional settling process after the two step solid-liquid separation process.

• Journal of Environmental Impact Assessment
• /
• v.23 no.2
• /
• pp.75-87
• /
• 2014

In order to analyze environmental impact of livestock manure and organic fertilizers, this study investigated livestock-breeding and pollution loads, the status of individual and public livestock manure treatment facilities, and the status of production, supply and components of compost and liquid fertilizers in the Nonsan area. Also, on a trial basis, this study investigated the life cycle of the environmental impact of livestock manure and its organic fertilizers on stream, groundwater, and agricultural soil. The results are as follows. Firstly, were detected the range of $0.13{\sim}1.32{\mu}g/L$ of As, $0.004{\sim}0.467{\mu}g/L$ of Cd and $0.5{\sim}9.2{\mu}g/L$ of Pb as a harmful substances which show lower concentrations than person preservation criteria of water qualities and aquatic ecosystem. However, it is not clear that heavy metals affect environment such as stream, groundwater and agricultural soil. Secondly, this influence could change according to investigation time and treatment efficiency. As were detected large amounts of persistent organic pollutants(e.g. $14.24{\sim}38.47{\mu}g/L$ of acetylsalicylic acid, $1.17{\sim}2.96{\mu}g/L$ of sulfamethazine, and $2.25{\sim}174.09{\mu}g/L$ of sulfathiazole) in effluent from livestock farms and small amounts of sulfathiazole($ND{\sim}1.63{\mu}g/L$) in the stream, it is necessary to monitor POPs at individual and public livestock manure treatment facilities. However, significant environmental impact did not appear at groundwater and agricultural soil in the test area supplied with liquid fertilizers. These results could be applied to investigate the environmental impact of livestock manure through a comprehensive livestock manure management information system.

• Journal of Animal Environmental Science
• /
• v.10 no.1
• /
• pp.1-10
• /
• 2004

A field-scale(8.6${\times}$2.5${\times}$2.4 m) and pilot-scale(1.39${\times}$0.89${\times}$0.89 m) thermophilic aerobic oxidation (TAO) units were installed to investigate the volume reduction efficiency of slurry, by varying the aeration and treatment temperature of swine manure, and the collected liquid was evaluated as a liquid fertilizer. In the field-scale unit, the aeration level and numbers of foam breakers made different effects on the slurry volume and temperature in the TAO system. The experiments were peformed for three cases, using different levels of aeration and numbers of foam breakers: Treat-A (aeration rate; 120 ㎥ air/hr using 2 air pumps and 2 foam breakers), Treat-B (aeration rate; 180 ㎥ air/hr using 3 air pumps and 3 foam breakers) and Treat-C (aeration rate; 180 ㎥ air/hr using 3 air pumps and 4 foam breakers). With the same input volume (5 ㎥/day) of swine manure slurry, the resulting liquid levels, temperatures and evaporation rates were 50∼100 cm, 31∼$64^{\circ}C$ and 55 $\ell/m^2$/day for Treat-A; 40∼90 cm, 29∼$52^{\circ}C$ and 75 $\ell/m^2$/day for Treat-B; and 40∼70 cm, 45∼$54^{\circ}C$ and 120.0 $\ell/m^2$/day for Treat-C. In the pilot-scale unit, semi-continuous flow of swine manure slurry was introduced. 50 $\ell$ every 2hr(T-1), 50 $\ell$ every 3hr(T-2), 40 $\ell$ every 2hr (T-3) and 60 $\ell$ every 4hr (T-4) within 24 hours, in order to find the maximum slurry volume reduction conditions.