• Asian-Australasian Journal of Animal Sciences
• /
• v.13 no.10
• /
• pp.1373-1376
• /
• 2000

The peroxidation status of tissues was estimated in broilers under acute or chronic heat stress ($32^{\circ}C$, 24 h, $5{\times}24h$) in the present study. The results showed that the lipid peroxide (LPO) concentrations in plasma and liver were elevated (p<0.05) by acute heat stress, and were not influenced in kidney (p>0.05). At the same time, no significant change of superoxide dismutase (SOD) activity in the liver, kidney or plasma was observed. Under chronic heat exposure, the SOD activity in liver was increased (p<0.05) and the LPO concentrations in the liver and plasma were restored to the normal levels. The LPO level in kidney was not affected by chronic heat stress (p>0.05), but SOD activity was significantly decreased (p<0.01). The results suggested that the peroxidation was induced by acute heat stress and disappeared along with the time of heat exposure, and the peroxidation reactions were different among tissues.

• The Journal of Dong Guk Oriental Medicine
• /
• v.8 no.1
• /
• pp.35-49
• /
• 1999

This study was made to investigate the antioxidative effects of Geagibokrounghwan on the hepatic and renal lesion induced by cholesterol in mouse. The normal group was fed basal diet and water ; control groups were fed basal diet containing 0.5% of cholesterol ; test groups were fed the Geagibokrounghwan extract($10m{\ell}/kg$) after fed basal diet containing 0.5% of cholesterol for 6 weeks. In the liver and kidney of control group, lipid peroxidation(LPO) was significantly increased, however, the activities of superoxidation dismutase(SOD) and catalase and the amount of glutathion(GSH) were significantly decreased. In the liver and kidney of test group, lipid peroxidation(LPO) was decreased significantly as compared with control group. Contrary to this, the activities of superoxide dismutase(SOD), catalase and the amount of glutathion(GSH) were significantly increased. These results indicate that Geagibokrounghwan revealed the antioxidant effects, which may reduce the hepatic and renal damage induced by cholesterol in mouse.

• Korean Journal of Environmental Biology
• /
• v.22 no.3
• /
• pp.387-393
• /
• 2004

Experiments were carried out to investigate the lipid peroxidation (LPO), superoxide dismutase (SOD) activity and histopathological change of hepatic tissue for rockfish, Sebastes schlegeli after feeding sub-chronic dietary Benzo(a)pyrene (BaP) in the concentration of 0 (control), 0.5, 1.0, 1.5, 2.0 mg $kg^{-1}$ dry food of diet for 30 days. In 2.0 mg $kg^{-1}$ dry food group, the significant increase of LPO was observed in all period, and SOD activity was incresed at 30 days significantly in the same concentration. In the histological investigation of liver, there was the swelling of hepatic cells at 10 days over the 1.0 mg $kg^{-1}$ dry food concentration. At 30 days Periodic acid-Schiff (PAS) positive granule was observed in the same group and at 20 days was observed in 2.0 mg $kg^{-1}$ dry food group. And there was necrosis of hepatic cell in some fish of 2.0 mg $kg^{-1}$ dry food group at 30 days.

• Journal of Acupuncture Research
• /
• v.17 no.3
• /
• pp.176-187
• /
• 2000

Objectives : This study was purposed to investigate the antioxidative effects of Paeoniae radix aqua-acupuncture solution(PR) on culture liver cell system, lipid peroxidation and antioxidative enzyme activities in tert-butyl hydroperoxide(t-BHP) treatmented conditions. Methods : Cultured normal rat liver cell(Ac2F) were prepared and incubated with or without PR(at 2% volume in culture medium). After 16~18hr, cells placed in DMEM medium without serum, and then incubated with 1mM t-BHP for 2hr. Viable cells were detected by MTT assay, and the levels of lipid peroxide(LPO) were measured by TBA method. And catalase activity was measured as the decrease in hydrogen peroxide absorbance at 240nm on spectrophotometer using 30mM hydrogen peroxide. Superoxide dismutase(SOD) were assayed by recording the inhibition of nitro blue tetrazolium reduction with xanthine and xanthine oxidase. Glutathione peroxidase(GPX) activity was determined by the modified coupled assay developed by Paglia and Lawrence. The reaction was started by addition of 2.2mM hydrogen peroxide as substrate. The change in absorbance at 340nm was measured for 1min on spectrophotometer. Glutathione-S-transferase(GST) activity was assayed with CDNB as substrate and enzyme activity of GST towards the glutathione conjugation of CDNB. Results : Cell killing was significantly enhanced by addition of t-BHP compared to those of untreated group. PR pretreated cell resisted the toxic effects of t-BHP. LPO levels of t-BHP treatment group were significantly higher than other groups. This increased level was significandy reduced by PR pretreatment. The t-BHP treatment resulted in a decrease of catalase, GPX and GST activities. By contrast, PR pretreatment markedly increased compare to those of untreated groups. Conclusions : T-BHP which can produce intracellular free radical was used for inducer of the peroxidation of cellular lipids. PR protected the cell death induced by t-BHP and significantly increased cell viabiliry in the normal rat liver cell, and showed effective inhibition of lipid peroxidation, and elevations of catalase, GPX and GST activities. These results suggested that PR might play a protective role in lipid peroxidation by free radicals.

• 대한방사선방어학회:학술대회논문집
• /
• 2011.04a
• /
• pp.116-117
• /
• 2011

• Korean Journal of Food Science and Technology
• /
• v.37 no.6
• /
• pp.976-982
• /
• 2005

Abilities of various edible plants and natural antioxidants to protect brain against oxidative damages were evaluated using brain homogenate of perfused Sprague-Dawley rat. Oxidative damage, expressed as lipid peroxidation (LPO), indicating total quantity of malondialdehyde and 4-hydroxyalkenal, increased from 4.1 to 6.9nmol/mg protein by treatment of $2.5{\mu}M$ ferrous sulfate and 7.5mM hydrogen peroxide as source of reactive oxygen species (ROS) on brain homogenate for 10min at $37^{\circ}C$ Mallow(88%) in leafy vegetables, small potato (93%) in root vegetables, green red pepper (76%) in fruit vegetables, and avocado (96%) in fruits showed highest LPO inhibition capacities. Ability of mushrooms decreased in order of nameko, shiitake, pine mushroom, oyster mushroom, and new type pine mushroom. Among natural antioxidants tested, (+)catechin (91%), (-)epigallocatechin gallate (85%), (-)epicatechin gallate (83%), and kaempferol(83%) showed high LPO inhibition capacities.

• Nutritional Sciences
• /
• v.9 no.1
• /
• pp.9-13
• /
• 2006

The differential effects of various fatty acids such as n-3 and n-6 types or degrees of unsaturation on the CYP2E1 induction and the production of lipid peroxidation (LPO) were investigated. The CYP2E1-transduced human hepatoma HepG2 cells (E47) were cultured in RPMI 1640 media containing different concentrations of various fatty acids up to 48 h incubation compared to 04 cells and CYP2E1-null cells. Treated fatty acids were linoleic acid (LA:n-6, C18:2), arachidonic acid (AA:n-6, C20:4) and docosahexaenoic acid (DHA:n-3, C22:6). The cell survival rate was decreased corresponding to the degree of unsaturation (LA>AA $\cong$DHA) and to LPO production in E47 and 04 cells. The four or five unsaturation degree of fatty acids, AA and DHA, caused time- and dose-dependent cell death in E47 cells but not as much as in C34 (without CYP2E1), suggesting an important role of CYP2E1 in the DHA mediated damage. In the levels of lipid peroxides (LPO), AA also elevated LPO by 3- and 5- fold compared to DHA or LA treated E47 cells. However, AA did not increase LPO until 48 h incubation in C34 cells. In conclusion, the polyunsaturated fatty acids induced CYP2E1 induction might be changed by the elevated levels of lipid peroxide (LPO) and oxidative stress through the connection of CYP2E1 and degrees of unsaturated fatty acids.

• Food Science and Biotechnology
• /
• v.18 no.2
• /
• pp.436-441
• /
• 2009

The final bio-metabolites of lipid peroxidation (LPO) such as 4-hydroxynonenal (4-HNE) and malondialdehyde (MDA) have been suggested to mediate the oxidative stress-linked pathological incidences. Natural phytochemicals such as polyphenolic compounds in green tea have been known in preventing the LPO induced cellular growth inhibition and apoptosis. We investigated that green tea ethanol extracts (GTE) inhibit LPO-induced apoptosis in ECV 304 cells. GTE had time- or dose-dependent anti-apoptotic effects as evidenced by changes in cell morphology, MTT assay, DNA fragmentation, LPO production, and the Western blotting for apoptotic expression. In the 4-HNE-induced apoptosis model, GTE $10-20{\mu}g/mL$ decreased cell death through decreasing LPO production. GTE protected 4-HNE induced apoptosis, as evidence with down regulation of mitochondrial signaling such as cytochrome C and caspase-3 activity. GTE increased bcl2, survival signaling protein, compared to 4-HNE alone within 6 hr incubation. Since polyphenols in GTE are effective antioxidants in endothelial ECV 304 cells, we suggested that natural polyphenols might be anti-atherosclerotic.

• Journal of Integrative Natural Science
• /
• v.15 no.4
• /
• pp.137-144
• /
• 2022

This paper presents the effect of the supramolecular complex of GA (Glycyrrhizic acid) and Mt(menthol) and GA: Mt (4: 1) obtained on their basis can restore functional dysfunction of the liver mitochondria in alloxan diabetes, ie, inhibit lipid peroxidation. The hypoglycemic activity and mitochondrial membrane stabilizing properties of the supramolecular compound GA: Mt (4: 1) in alloxan diabetes were more pronounced than those of menthol, GA and its GK: Mt (2: 1) and GA: Mt (9: 1) compounds. According to the results obtained, the concentration of GA did not affect the peroxidation of lipid membranes of the liver mitochondria. However, a concentration of 15 μM of GA was found to reduce LPO (lipid peroxidation) formed by the effect of Fe²+ / ascorbate on the mitochondrial membrane by 58.0 ± 5.0% relative to control. The inhibitory effect of GA and its supramolecular compounds in different proportions with menthol on the peroxidation of lipids in rat heart and brain tissue has been studied

• Bulletin of Food Technology
• /
• v.11 no.4
• /
• pp.61-69
• /
• 1998

간 독성 물질인 2-Nitropropane (2-NP)을 쥐의 복강내로 주사한 후에 조직에서의 지질산화(lipid peroxidation (LPO), malondialdehyde 와 4-hydroxyalkenal의 생산량)와 간 독성의 지표로서 혈청내의 soritol dehydrogenase(SDH) 활성을 측정하였다. 수많은 독성 물질에 대하여 방어효과를 보이는 멜라토닌(melatonin)을 2-NP 투여 30분전에 주사하여 2-NP에 대한 방어효과를 조사하였다. 2-NP 투여시에 LPO와 SDH 활성은 시간 (0, 4, 8, 24h) 및 2-NP의 농도 (0, 1, 2, 4mmol/kg)에 따라 증가하였고, 4mmol/kg의 2-NP을 투여한 24시간 후에는 간 (P<0.001), 폐 (P<0.05), 신장(P<0.001)에서 LPO가 유의적으로, 혈청에서의 SDH 활성은 470배 증가하였다. 멜라토닌을 농도에 따라 투여시(2.5, 5, 10 mg/kg) 간, 폐 신장에서 LPO가 간에 대하 발암성 물질인 2-NP가 지질산화도 유도 할 수 있으며, 약리적인 수준의 멜라토닌이 2-NP의 독성을 감소시킬 수 있음을 나타낸다.