• 한국식품영양학회지
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• 제32권1호
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• pp.27-32
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• 2019

The objective of this study was to evaluate novel usability as natural anti-obesity supplement of Selaginella tamariscina extract. The total phenol contents and total flavonoid contents were $60.29{\pm}3.11GAE\;mg/g$ and $14.90{\pm}0.34QE\;mg/g$, respectively. To evaluate anti-obesity activity of Selaginella tamariscina extract, pancreatic lipase inhibition activity as well as its inhibition effect of lipid accumulation in adipocytes were conducted by Oil Red O staining and lipolysis assay. The result of pancreatic lipase inhibition activity of S. tamariscina extract showed a wide range between 40 and 73% dose dependently. While the incubation of 3T3-L1 cells with S. tamariscina extract inhibited differentiation of preadipocytes and reduced lipid accumulation, the level of released free glycerol into culturing medium was increased in multiple concentrations. These results showed that S. tamariscina extract inhibit adipogenesis and pancreatic lipase activity. Thus, S. tamariscina extract can be a candidate for regulating lipid accumulation in obesity.

• 한국축산식품학회지
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• 제34권6호
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• pp.836-843
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• 2014

Obesity, a condition in which an abnormally large amount of fat is stored in adipose tissue, causing an increase in body weight, has become a major public health concern worldwide. The purpose of this study was to optimize the process for fermented milk for the production of a functional product with an anti-obesity effect by using Lactobacillus plantarum Q180 isolated from human feces. We used a 3-factor, 3-level central composite design (CCD) combined with the response surface methodology (RSM). Concentration of skim milk powder (%, $X_1$), incubation temperature ($^{\circ}C$, $X_2$), and incubation time (h, $X_3$) were used as the independent factors, whereas pH (pH, $Y_1$), anti-lipase activity (%, $Y_2$) and anti-adipogenetic activity (%, $Y_3$) were used as the dependent factors. The optimal conditions of fermented milk for the highest anti-lipase and anti-adipogenetic activity with pH 4.4 were the 9.5% of skim milk powder, $37^{\circ}C$ of incubation temperature, 28 h of incubation time. In the fermentation condition, the predicted values of pH, anti-lipase activity and anti-adipogenetic activity were 4.47, 55.55, and 20.48%, respectively. However, the actual values of pH, anti-lipase activity and anti-adipogenetic activity were 4.50, 52.86, and 19.25%, respectively. These results demonstrate that 9.5% of skim milk powder and incubation at $37^{\circ}C$ for 28 h were the optimum conditions for producing functional fermented milk with an anti-obesity effect.

• 한국식품영양과학회지
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• 제40권2호
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• pp.308-315
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• 2011

연구는 강원도 자생 산채류의 다양한 약리 기능 중 ${\alpha}$-glucosidase, ${\alpha}$-amylase, lipase 효소 저해활성이 우수한 식물소재를 탐색하는데 있다. 강원도 양구, 평창, 태백에서 자생하고 있는 산채 90종을 채집하여 물과 에탄올로 각각 추출하였다. 항비만 활성 탐색은 ${\alpha}$-amylase, ${\alpha}$-glucosidase, lipase 저해활성을 검정하였다. 그 결과 산채 90종 중 ${\alpha}$-amylase 활성 저해능이 높은 식물로 에탄올 추출물 3종, 물추출물 2종, ${\alpha}$-glucosidase 활성 저해능이 높은 식물로 에탄올 추출물 13종, 물 추출물 3종, lipase 활성 저해능이 높은 식물로 에탄올 추출물 7종, 물 추출물 1종이 선발되었다. 향후 본 연구결과는 비만과 당뇨 등 대사성 질환에 대해 예방 혹은 치료 효과가 있는 기능성식품으로 개발하는데 있어 좋은 기초자료로 활용될 것으로 기대된다.

• 한국식품저장유통학회지
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• 제21권3호
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• pp.421-426
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• 2014

지모를 70% methanol로 침지 추출하여 얻어진 추출물에 대해 n-hexane, EtOAc 및 n-BuOH로 순차 용매 분획하였고, 얻어진 결과물에 대하여 DPPH 및 $ABTS^+$ radical 소거능 및 pancreatic lipase 저해활성을 평가하였다. DPPH 라디칼 소거능은 페놀성 화합물의 함량이 상대적으로 높은 n-BuOH 층의 1000 mg/ml의 시험농도에서는 91.2%의 라디칼 소거능을 확인하였고, 지모 추출물에 존재하는 페놀성 화합물이 라디칼 소거능과의 연관성을 시사하였다. 또한 $ABTS^+$ 라디칼 소거능은 EtOAc층이 $IC_{50}=20.5{\pm}1.7mg/ml$, n-BuOH 층이 $IC_{50}=50.5{\pm}0.7mg/ml$의 활성이 확인 되었고, 강한 활성물질의 존재가 시사되었다. 또한, pancreatic lipase 저해활성을 측정한 결과, 강한 $ABTS^+$ 라디칼 소거능을 나타낸 EtOAc 층의 $IC_{50}$은 $31.3{\pm}0.1mg/ml$의 저해율을 나타내었으며 이는 대조군인 orlistat에 비해 약하나 단일물질로 정제할 경우 더욱 강한 효능의 화합물이 존재할 가능성이 있음을 보여준다. 오일의 형태로 얻어진 n-hexane 분획층은 항산화 활성 및 pancreatic lipase 저해 활성을 거의 나타내지 않았다. 향후 이들 활성물질 동정을 통한 활성 기작에 대한 연구가 필요하며 본 연구결과는 보다 우수한 라디칼 소거능 및 pancreatic lipase 저해능을 가지는 새로운 선도화합물 발굴을 위한 기초자료로 활용가능하리라 사료된다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제9권6호
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• pp.465-470
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• 2004

Optimal conditions for the in situ immobilization of lipase in aldehyde-silica packed columns, via reductive amination, were investigated. A reactant mixture, containing lipase and sodium borohydride (NaCBH), was recirculated through an aldehyde-silica packed column, such that the covalent bonding of the lipase, via amination between the amine group of the enzyme and the aldehyde terminal of the silica, and the reduction of the resulting imine group by NaCBH, could occur inside the bed, in situ. Mobile phase conditions in the ranges of pH $7.0{\~}7.8$, temperatures between $22{\~}28^{circ}C$ and flow rates from $0.8{\~}1.5\;BV/min$ were found to be optimal for the in situ immobilization, which routinely resulted in an immobilization of more than 70 mg­lipase/g-silica. Also, the optimal ratio and concentration for feed reactants in the in situ immobilization: mass ratio [NaCBH]/[lipase] of 0.3, at NaCBH and lipase concentrations of 0.75 and 2.5 g/L, respectively, were found to display the best immobilization characteristics for concentrations of up to 80 mg-lipase/g-silica, which was more than a 2-fold increase in immobilization compared to that obtained by batch immobilization. For tributyrin hydrolysis, the in situ immobilized lipase displayed lower activity per unit mass of enzyme than the batch-immobilized or free lipase, while allowing more than a $45\%$ increase in lipase activity per unit mass of silica compared to batch immobilization, because the quantity of the immobilization on silica was aug­mented by the in situ immobilization methodology used in this study.

• 생명과학회지
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• 제23권10호
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• pp.1246-1251
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• 2013

산업공단지역의 폐수에서 분리한 유기용매 내성 Pseudomonas sp. BCNU 154 리파아제의 최적조건은 $37^{\circ}C$, pH8로 조사되었다. BCNU 154의 crude 리파아제는 toluene에서 2시간 반응시 효소활성 약 6.01 U/ml (117.53%)로 가장 안정한 것으로 나타났다. 한편 $Ca^{2+}$, $Mg^{2+}$, $NH_4{^+}$, $Na^+$ 이온 및 triton X-100은 효소를 활성화시킨 반면에 $Ba^{2+}$, $Hg^{2+}$ 및 $Zn^{2+}$ 이온은 효소활성을 억제하였다. Pseudomonas sp. BCNU 154 리파아제는 상용 고정화 효소인 Novozym 435와 비교해서도 안정한 활성을 보였다. 그러므로 유기용매 내성 리파아제는 별도의 고정화 처리없이도 화학산업공정에서 가능성 있는 whole cell 생물촉매로서 유용할 것으로 판단된다.

• Journal of Microbiology and Biotechnology
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• 제10권1호
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• pp.99-102
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• 2000

To establish optimal conditions for esterification by Candida cylindracea, lipase reactions were performed simultaneously, separately, or individually in the varying initial rates of $0.014-0.060\mu$mole free fatty acids consumed min-1g-1. The reactants which were conditioned at aw of 0.12 gave the highest initial rate of esterifying $0.060\mu$mole free fatty acids consumed min-1g-1. These results suggest that the esterifying activity of lipase in an organic system depends on the transfer of available water within the reaction system.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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• pp.226.1-226.1
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• 2003

Pancreatic lipase-inhibitory activity of the rhizome of Alpinia officinarum (AO) and its antihyperlipidemic activity were measured. When water extract of AO was stepwise fractionated with organic solvents, ethylacetate fraction exhibited the most potent inhibition. From it, Diarylheptanoid was isolated as an inhibitor of pancreatic lipase and we investigated its in vitro inhibitory effect of lipase activity and in vivo antihyperlipidemic effect. (omitted)

• Journal of Microbiology and Biotechnology
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• 제18권4호
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• pp.735-741
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• 2008

In our previous study, a surfactant-coated Candida rugosa lipase immobilized in microemulsion-based organogels was exploited for the synthesis of ethyl isovalerate. In the present study, we are focusing on the effective reuse of lipase immobilized in microemulsion-based organogels (MBGs) in terms of retainment of the catalytic activity. As water is one of the co-products in esterification reactions, the removal of water becomes a priority to allow the reaction to work in the forward direction and to prevent back hydrolysis. Taking this fact into consideration, the lipase-containing microemulsion-based organogels were given pretreatment and/or several intermittent treatments with dry reverse micellar solution of AOT in organic solvent during repeated cycles of ester synthesis. The pretreated MBGs with dry reverse micellar solution exhibited lower water content and higher initial rates of esterification in comparison with untreated freshly prepared MBGs. The esterification efficiency of untreated MBGs started decreasing after 5 cycles of reuse and was almost completely lost by the end of the $8^{th}$ cycle. In contrast, pretreated MBGs exhibited a gradual decrease in esterification efficiency after 5 cycles and retained about 80% of the initial activity at the end of the $8^{th}$ cycle. The intermittent treatment of MBGs after every 3 cycles resulted in enhanced reusability of immobilized lipase for up to 9 cycles without significant loss in esterification activity, after which it resulted in a slow decrease in activity with about 27% lower activity at the end of the $12^{th}$ cycle. Furthermore, the treatment conditions such as concentration of AOT in liquid dessicant and time of treatment were optimized with respect to our system. The granulated MBGs proved to be better in terms of initial esterification rates (1.2-fold) as compared with the pelleted MBGs.

• 한국약용작물학회지
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• 제20권1호
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• pp.1-7
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• 2012

This study was performed to develop new anti-obesity agents for functional foods from medicinal plants and herbs. Methanol extracts from 560 kinds of medicinal plants and herbs, and their lipase inhibitory activities were investigated. Methanol extract from Desmodium oxyphyllum Dc showed the highest lipase inhibitory activity at 74.2%. This lipase inhibitory activity was maximally extracted when powder of Desmodium oxyphyllum Dc was treated with 80% methanol (1 : 20) for 48 h.