• Journal of Electrical Engineering and Technology
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• v.13 no.2
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• pp.918-927
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• 2018

Based on the actual operating environment of transformer, the aging tests of nitrile butadiene rubber (NBR) were conducted systematically under four conditions: in air, in transform oil, under compression in air and under compression in transform oil to studythe effect of high temperature, transform oil and compression stress simultaneously on the thermal aging behaviors of nitrile butadiene rubber and predict the lifetime. The effects of liquid media and compression stress simultaneously on the thermal aging behaviors of nitrile butadiene rubber were studied by using characterization methods such as IR spectrosc-opy, thermogravimetric measurements, Differential Scanning Calorimetry (DSC) measurements and mechanical property measurements. The changes in physical properties during the aging process were analyzed and compared. Different aging conditions yielded materials with different properties. Aging at $70^{\circ}C$ under compression stress in oil, the change in elongation at break was lower than that aging in oil, but larger than that aging under compression in air. The compression set or elongation at break as evaluation indexes, 50% as critical value, the lifetime of NBR at $25^{\circ}C$ was predicted and compared. When aging under compression in oil, the prediction lifetime was lower than in air and under compression in air, and in oil. It was clear that when predicting the service lifetime of NBR in oil sealing application, compression and media liquid should be involved simultaneously. Under compression in oil, compression set as the evaluation index, the prediction lifetime of NBR was shorter than that of elongation at break as the evaluation index. For the life prediction of NBR, we should take into account of the performance trends of NBR under actual operating conditions to select the appropriate evaluation index.

• Korean Journal of Acupuncture
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• v.37 no.1
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• pp.1-13
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• 2020

Objectives : To analyze the contents of ≪Lingshu·Jingjin≫ and study the standard posture in context of Korean Medicine. Methods : Analyzed the terms related to the name, body region and orientation of Three Yin and Three Yang used in ≪Lingshu·Jingjin≫ to deduce the standard posture of the description. ≪Lingshu·Jingmai≫ was used as supplementary data. Results : The term "Three Yin and Three Yang" in ≪Lingshu·Jingjin≫ is used to indicate regions of the human body, and based on this, terms with orientation were used. Just like 'anatomical position', there is a standard posture in Korean Medicine, and it may seem to be similar overall, but there is a difference in posture in the upper extremity. In ≪Lingshu·Jingjin≫, Greater Yang is the dorsal region, Lesser Yang is the lateral surface region, Yang Brightness is the anterior surface region of the human body. In the body trunk, Three Yin refers to the inner parts of the human body. However, in the lower extremity, Three Yin refers to the medial surface of the legs. The name of the individual Meridian-muscle was given following the region corresponding to Three Yin and Three Yang. In ≪Lingshu·Jingjin≫, there is a basic posture that became the standard posture derived from the description. In an upright standing position, the feet face forward, the fingers naturally extended, and the back of the hand faces outward. The fact that the posture of the thumb is naturally extended is especially reflected in ≪Lingshu·Jingjin≫. This is clearly different from the "anatomical position" and as it can be the base of all areas of acupuncture, it is suggested that it be defined as the "Standard Position of Acupuncture Medicine". Conclusions : Based on our analysis, we suggest the "Standard Position of Acupuncture Medicine" as an upright standing position, with the feet facing forward, the fingers naturally extended, the back of the hand facing outward, and the thumb naturally extended.

• BMB Reports
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• v.52 no.9
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• pp.566-571
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• 2019

Lymphoma is one of the most curable types of cancer. However, drug resistance is the main challenge faced in lymphoma treatment. Peroxisomal acyl-CoA oxidase 1 (ACOX1) is the rate-limiting enzyme in fatty acid ${\beta}$-oxidation. Deregulation of ACOX1 has been linked to peroxisomal disorders and carcinogenesis in the liver. Currently, there is no information about the function of ACOX1 in lymphoma. In this study, we found that upregulation of ACOX1 promoted proliferation in lymphoma cells, while downregulation of ACOX1 inhibited proliferation and induced apoptosis. Additionally, overexpression of ACOX1 increased resistance to doxorubicin, while suppression of ACOX1 expression markedly potentiated doxorubicin-induced apoptosis. Interestingly, downregulation of ACOX1 promoted mitochondrial location of Bad, reduced mitochondrial membrane potential and provoked apoptosis by activating caspase-9 and caspase-3 related apoptotic pathway. Overexpression of ACOX1 alleviated doxorubicin-induced activation of caspase-9 and caspase-3 and decrease of mitochondrial membrane potential. Importantly, downregulation of ACOX1 increased p73, but not p53, expression. p73 expression was critical for apoptosis induction induced by ACOX1 downregulation. Also, overexpression of ACOX1 significantly reduced stability of p73 protein thereby reducing p73 expression. Thus, our study indicated that suppression of ACOX1 could be a novel and effective approach for treatment of lymphoma.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.4
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• pp.2325-2331
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• 2013

Objective: To detect effects of plumbagin on proliferation and apoptosis in non-small cell lung cancer cell lines, and investigate the underlying mechanisms. Materials and Methods: Human non-small cell lung cancer cell lines A549, H292 and H460 were treated with various concentrations of plumbagin. Cell proliferation rates was determined using both cell counting kit-8 (CCK-8) and clonogenic assays. Apoptosis was detected by annexin V/propidium iodide double-labeled flow cytometry and TUNEL assay. The levels of reactive oxygen species (ROS) were detected by flow cytometry. Activity of NF-${\kappa}B$ was examined by electrophoretic mobility shift assay (EMSA) and luciferase reporter assay. Western blotting was used to assess the expression of both NF-${\kappa}B$ regulated apoptotic-related gene and activation of p65 and $I{\kappa}B{\kappa}$. Results: Plumbagin dose-dependently inhibited proliferation of the lung cancer cells. The IC50 values of plumbagin in A549, H292, and H460 cells were 10.3 ${\mu}mol/L$, 7.3 ${\mu}mol/L$, and 6.1 ${\mu}mol/L$ for 12 hours, respectively. The compound concentration-dependently induced apoptosis of the three cell lines. Treatment with plumbagin increased the intracellular level of ROS, and inhibited the activation of NK-${\kappa}B$. In addition to inhibition of NF-${\kappa}B$/p65 nuclear translocation, the compound also suppressed the degradation of $I{\kappa}B{\kappa}$. ROS scavenger NAC highly reversed the effect of plumbagin on apoptosis and inactivation of NK-${\kappa}B$ in H460 cell line. Treatment with plumbagin also increased the activity of caspase-9 and caspase-3, downregulated the expression of Bcl-2, upregulated the expression of Bax, Bak, and CytC. Conclusions: Plumbagin inhibits cell growth and induces apoptosis in human lung cancer cells through an NF-${\kappa}B$-regulated mitochondrial-mediated pathway, involving activation of ROS.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.4
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• pp.562-568
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• 2017

Objective: The hull attached to the barley kernel can be mechanically removed thus reducing the fiber content of the barley. This experiment was carried out to evaluate the effects of partial dehulling on the nutrient digestibility as well as the digestible energy (DE) and metabolizable energy (ME) content of barley in pigs. Methods: Two hulled barley samples (high fiber barley [HF] and low fiber barley [LF]) with either high or low fiber contents were obtained from the Hubei and Jiangsu Provinces of China. A portion of the two barleys was mechanically dehulled (dehulled high fiber barley [DHF] and dehulled low fiber barley [DLF]). Thirty barrows (initial $BW=31.5{\pm}3.2kg$) were assigned to one of five diets in a randomized complete block design. The five diets consisted of 96.9% corn, HF, LF, DHF, or DLF supplemented with 3.1% minerals and vitamins. Each diet was fed to six barrows housed in individual metabolism crates for a 10-d acclimation period followed by a 5-day total but separate collection of feces and urine. Results: The daily loss of gross energy (GE) in feces was lower (p<0.01) for pigs fed DHF than for those fed HF. The daily N intake and fecal N loss were lowest (p<0.01) for pigs fed the corn diet. The DE and ME as well as the apparent total tract digestibility (ATTD) of dry matter, GE, organic matter, neutral detergent fiber (NDF) and acid detergent fiber (ADF) of DHF or DLF were higher (p<0.01) than the values in HF and LF, respectively while the values except the ATTD of NDF and ADF in DLF were higher (p<0.01) than the values in DHF and were comparable to corn. Conclusion: The DE and ME contents as well as the ATTD of nutrients in both DHF and DLF barley were improved compared with the HF and LF barley. Moreover, the nutritive value of DLF barley was comparable to the yellow-dent corn used in the study.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.12
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• pp.7085-7090
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• 2013

Objective: Published data have shown that microRNAs (miRNAs) could play a potential role as diagnostic and prognostic indicators in cancers. Data for the predictive value of microRNA-155 are inconclusive. The aim of the present analysis was therefore to evaluate the role of miR-155 in prognosis for patients with a variety of carcinomas. Methods: Relevant studies were identified by searching PubMed and EMBASE. Data were extracted from studies comparing overall survival (OS), recurrence-free survival (RFS) or cancer-specific survival (CSS) in patients with carcinoma with higher miR-155 expression and those with lower levels. The pooled hazard ratios (HRs) and 95% confidence intervals (CIs) of miR-155 for clinical outcome were calculated. Results: A total of 15 studies were included. The pooled hazard ratio (HR) for OS of higher miR-155 expression in cancerous tissue was 1.89 (95% CI: 1.20-2.99, P=0.006), which could markedly predict poorer survival in general cancer. For RFS/CSS, elevated miR-155 was also associated with poor prognosis of cancer (HR=1.50, 95% CI: 1.10-2.05, P=0.01). On subgroup analysis, the pooled HR for OS in non-small cell lung cancer (NSCLC) was 2.09 (95% CI: 0.68-6.41, P > 0.05), but for RFS/CSS was 1.28 (95% CI: 1.05-1.55, P=0.015), with statistical significance; the pooled HRs for OS and RFS/CSS in digestive system neoplasms were 3.04 (95% CI: 1.48-6.24, P=0.003) and 2.61 (95% CI: 1.98-3.42, P<0.05), respectively. Conclusions: The results indicated that the miR-155 expression level plays a prognostic role in patients with cancer, especially NSCLCs and digestive system carcinomas.

• Journal of Korean Medical classics
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• v.24 no.2
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• pp.13-22
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• 2011

"Caraka-sa$\d{m}$hit$\bar{a}$ (闍羅迦集)" and "Su$\'{s}$ruta-sa$\d{m}$hit$\bar{a}$(妙聞集)" are the most important medical scriptures in India and are called the three scriptures of Ayuverda including "Astanga-hrdaya-sa$\d{m}$hit$\bar{a}$(八心集)" (which integrates and summarizes internal medicine and surgery.) that is scribed in 8C. Scriptures that have been told today are deemed to be written in 3 ~ 4C. The three scriptures of Ayuverda and "Yellow Emperor's Canon Internal Medicine(黃帝內經)" are very similar in historical position, transmission and separation and addition, pattern and importance of contents, etc, from the aspect of formation and transmission of scriptures when compared with herb medicine. The characteristics that "SuWen(素問)" concentrates on medical theory and "LingShu(靈樞)" focuses on acupuncture therapy are similar with the characteristics that "Caraka-sa$\d{m}$hit$\bar{a}$" values internal medicine and "Su$\'{s}$ruta-sa$\d{m}$hit$\bar{a}$" appreciates surgery. The chapter 8 of "Caraka-sa$\d{m}$hit$\bar{a}$ (闍羅迦集)" is called as 'a volume of completion (or a volume of success)' and describes details of 'pancakarma (which means five representative treatment methods, that is successful regulations of two kinds of basti(灌腸), $\'{s}$irsavirecana(油劑), virecana(下劑), vamana(吐劑). Therefore, the author compares summery of 'internal therapy' of Indian medicine and drug treatment method with Method Of 'Sweat, Vomit And Diarrhea of the representative treatment in herb medicine, based on "RuMenShiQin(儒門事親)" of ZhangZiHe(張子和).

• Biomolecules & Therapeutics
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• v.24 no.2
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• pp.132-139
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• 2016

The endothelial-mesenchymal transition (EndMT) is known to be involved in the transformation of vascular endothelial cells to mesenchymal cells. EndMT has been confirmed that occur in various pathologic conditions. Transforming growth factor ${\beta}1$ (TGF-${\beta}1$) is a potent stimulator of the vascular endothelial to mesenchymal transition (EMT). Aspirin-triggered resolvin D1 (AT-RvD1) has been known to be involved in the resolution of inflammation, but whether it has effects on TGF-${\beta}1$-induced EndMT is not yet clear. Therefore, we investigated the effects of AT-RvD1 on the EndMT of human umbilical vein vascular endothelial cells line (HUVECs). Treatment with TGF-${\beta}1$ reduced the expression of Nrf2 and enhanced the level of F-actin, which is associated with paracellular permeability. The expression of endothelial marker VE-cadherin in HUVEC cells was reduced, and the expression of mesenchymal marker vimentin was enhanced. AT-RvD1 restored the expression of Nrf2 and vimentin and enhanced the expression of VE-cadherin. AT-RvD1 did also affect the migration of HUVEC cells. Inhibitory ${\kappa}B$ kinase 16 (IKK 16), which is known to inhibit the NF-${\kappa}B$ pathway, had an ability to increase the expression of Nrf2 and was associated with the inhibition effect of AT-RvD1 on TGF-${\beta}1$-induced EndMT, but it had no effect on TGF-${\beta}1$-induced EndMT alone. Smad7, which is a key regulator of TGF-${\beta}$/Smads signaling by negative feedback loops, was significantly increased with the treatment of AT-RvD1. These results suggest the possibility that AT-RvD1 suppresses the TGF-${\beta}1$-induced EndMT through increasing the expression of Smad7 and is closely related to oxidative stress.

• Molecules and Cells
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• v.26 no.6
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• pp.536-547
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• 2008

Anthocyanidin synthase (ANS, leucoanthocyanidin oxygenase), a 2-oxoglutarate iron-dependent oxygenase, catalyzed the penultimate step in the biosynthesis of the anthocyanin class of flavonoids, from the colorless leucoanthocyanidins to the colored anthocyanidins. The full-length cDNA and genomic DNA sequences of ANS gene (designated as GbANS) were isolated from Ginkgo biloba for the first time. The full-length cDNA of GbANS contained a 1062-bp open reading frame (ORF) encoding a 354-amino-acid protein. The genomic DNA analysis showed that GbANS gene had three exons and two introns. The deduced GbANS protein showed high identities to other plant ANSs. The conserved amino acids (H-X-D) ligating ferrous iron and residues (R-X-S) participating in 2-oxoglutarate binding were found in GbANS at the similar positions like other ANSs. Southern blot analysis indicated that GbANS belonged to a multi-gene family. The expression analysis by real-time PCR showed that GbANS expressed in a tissue-specific manner in G. biloba. GbANS was also found to be up-regulated by all of the six tested abiotic stresses, UV-B, abscisic acid, sucrose, salicylic acid, cold and ethylene, consistent with the promoter region analysis of GbANS. The recombinant protein was successfully expressed in E. coli strain with pET-28a vector. The in vitro enzyme activity assay by HPLC indicated that recombinant GbANS protein could catalyze the formation the cyanidin from leucocyanidin and conversion of dihydroquercetin to quercetin, suggesting GbANS is a bifunctional enzyme within the anthocyanidin and flavonol biosynthetic pathway.

• Korean Journal of Acupuncture
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• v.23 no.4
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• pp.27-47
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• 2006

Objectives : The aim of this study was to analyze how to treat various symptoms through the combination of Yoelgyoel $(LU_7)$ and Johae $(KI_6)$, according to reviewing the contents and data since Ling Shu (靈樞經) to recent literatures including thirty-five medical books. Methods : It was arranged and considered that the location, needling, and symptoms of each acupoint were described in various literatures before the publication of Chim Kyung Ji Nam (鍼經指南). Through various literature since the Publication of Chim Kyung Ji Nam, it was examined how to be recognized and be referred about Yoelgyoel $(LU_7)$ and Johae $(KI_6)$. Results and Conclusions : The location of Yoelgyoel is the superior 1.5cun at wrist joint striation, medial of extensor carpi radialis longus; the location of Johae is the depression part under foot medial condyle. Yoelgyoel is often used for respiratory organ disease, urinary organ disease, neuopsychiatory disease, musculoskeletal system disease; Johae is often used for urinary organ disease, circulatory organ disease. At Chim Kyung Ji Nam, Yoelgyoel is often used for thoraco-abdominal Pain, gynecological disease, digestive organ disease; Johae is often used for abdominal pain, gynecological disease, digestive organ disease. Therefore, these points are used together for general internal disease. As well, these are not directly continuous with Previous literatures from Chim Kyung Ji Nam. The combination of Yoelgyoel and Johae have been developed to the three categories as follows; it is quoted from as it is; it is reconstructed in the form of song; it has new symptoms enlarged. Consequently, the combination of Yoelgyoel and Johae was not bind to the rule of Up-Bottom harmony (上下配合), but asserted for the rule of Ju-Eng harmony (主應配合), which add specific acupoints to e combination of Yolgyol and Chohae as the complication of the symptoms.