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Optimization of cultivation conditions for pullulan production from Aureobasidium pullulans MR by response surface methodology (반응표면분석법을 이용한 Aureobasidium pullulans MR의 풀루란 생산을 위한 배양 조건 최적화)

  • Jo, Hye-Mi;Kim, Ye-Jin;Yoo, Sang-Ho;Kim, Chang-Mu;Kim, KyeWon;Park, Cheon-Seok
    • Korean Journal of Food Science and Technology
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    • v.53 no.2
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    • pp.195-203
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    • 2021
  • Aureobasidium pullulans, a black yeast, produces pullulan, a linear α-glucan composed of maltotriose repeating units linked by α(1→6)-glycosidic linkages. Pullulan can be widely used in food, cosmetic, and biotechnology industries. In this study, we isolated eight strains of A. pullulans from Forsythia koreana, Magnolia kobus DC., Spiraea prunifolia var. simpliciflora, Cornus officinalis, Cerasus, and Hippophae rhamnoides. Among them, A. pullulans MR was selected as the best pullulan producer. The effects of a carbon source, a nitrogen source, and pH on pullulan production were examined. The optimal cultivation conditions for pullulan production by A. pullulans MR were determined by response surface methodology as 15% sucrose, 0.4% soy peptone, and an initial pH of 7 at 26℃. Under these conditions, the predicted pullulan production was 47.6 g/L, which was very close to the experimental data (48.9 g/L).

Study on the Application of Ultrasound Traits as Selection Trait in Hanwoo (한우 선발형질로써 초음파 형질의 활용방안 연구)

  • Choi, Tae Jeong;Choy, Yun Ho;Park, Byoungho;Cho, Kwang Hyun;Alam, M;Kang, Ha Yeon;Lee, Seung Soo;Lee, Jae Gu
    • Journal of agriculture & life science
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    • v.51 no.2
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    • pp.117-126
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    • 2017
  • Hanwoo young bulls are selected based on performance test using the weight at 12 months and pedigree index comprising marbling score. Pedigree index was not based on the progeny tested data but the breeding value of the proven bulls; resulting a lower accuracy. The progeny testing of the young bulls was categorized into testing at farm and at the test station. The farm tested data was difficult to compare with those from test station data. Farm tested bulls had different slaughter ages than those for test station bulls. Therefore, this study had considered a different age at slaughter for respective records on ultrasound traits. Records on body weight at 12 months, ultrasound measures at 12 and 24 months(uIMF, uEMA, uBFT, and uRFT), and carcass traits(CWT, EMA, BFT, and MS) were collected from steers and bulls of Hanwoo national improvement scheme between 2008 and 2013. Fixed effects of batch, test date, test station, personnel for measurement, personnel for judging, and a linear covariate of weight at measurement were fitted in the animal models for ultrasound traits. The ranges of heritability estimates of the ultrasound traits at 12 and 24 months were 0.21-0.43 and 0.32-0.47, respectively. Ultrasound traits at 12 and 24 months between similar carcass traits was genetically correlated at 0.52-0.75 and 0.86-0.89, respectively.

Development of Correction Formulas for KMA AAOS Soil Moisture Observation Data (기상청 농업기상관측망 토양수분 관측자료 보정식 개발)

  • Choi, Sung-Won;Park, Juhan;Kang, Minseok;Kim, Jongho;Sohn, Seungwon;Cho, Sungsik;Chun, Hyenchung;Jung, Ki-Yuol
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.24 no.1
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    • pp.13-34
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    • 2022
  • Soil moisture data have been collected at 11 agrometeorological stations operated by The Korea Meteorological Administration (KMA). This study aimed to verify the accuracy of soil moisture data of KMA and develop a correction formula to be applied to improve their quality. The soil of the observation field was sampled to analyze its physical properties that affect soil water content. Soil texture was classified to be sandy loam and loamy sand at most sites. The bulk density of the soil samples was about 1.5 g/cm3 on average. The content of silt and clay was also closely related to bulk density and water holding capacity. The EnviroSCAN model, which was used as a reference sensor, was calibrated using the self-manufactured "reference soil moisture observation system". Comparison between the calibrated reference sensor and the field sensor of KMA was conducted at least three times at each of the 11 sites. Overall, the trend of fluctuations over time in the measured values of the two sensors appeared similar. Still, there were sites where the latter had relatively lower soil moisture values than the former. A linear correction formula was derived for each site and depth using the range and average of the observed data for the given period. This correction formula resulted in an improvement in agreement between sensor values at the Suwon site. In addition, the detailed approach was developed to estimate the correction value for the period in which a correction formula was not calculated. In summary, the correction of soil moisture data at a regular time interval, e.g., twice a year, would be recommended for all observation sites to improve the quality of soil moisture observation data.

Soon Chul Jung's Melody Development Technique from the Viewpoint of Motive Unit Analysis (모티브(Motive) 단위 분석 관점으로 바라본 정순철 동요의 선율 전개기법)

  • Son, Mi-Jin;Chung, Jae-Youn
    • Journal of Korea Entertainment Industry Association
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    • v.15 no.2
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    • pp.35-46
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    • 2021
  • The purpose of this research is to analyze Soon Chul Jung's children's songs, who is one of the four great children's songwriters in Korea along with Geuk Young Yoon, Nan Pa Hong and Tae Jun Park. Soon Chul Jung has contributed by actively participate into children's movement at Japanese colonial period through writing self-composed children's songs. Since children's songs are made for the age group without the sense of sound, children's songs necessitate 'easy to sing-along' melody writing process. Like so, when writing the melody that are easy to be sang along, utilization of development technique using a unit called motive can be the potent way. This method has an advantage of being sang more with ease if actively implemented, and can be done by utilizing the smallest unit of the melody called motive, especially by lightly transform and repeat the unit, consequently structuring the song altogether. To investigate and analyze this motive-based development technique of Soon Chul Jung's songs, 38 out of 40 written songs considered eligible for the research were confirmed and chosen from 'Soon Chul Jung's bibliography and choreography' and each songs were analyzed based on 17 different development techniques which were standardized in the textbook 'Melody in Songwriting' adopted as a textbook of the Berklee college of Music, and also compared these song-writing pipelines with other great songwriters' representatives mentioned above. As a result, it was almost linear that the song's pitch variation in combination with thinning or extension were used frequently and repeatedly, otherwise implicating melody duplication for more than 2 bars and motive repetition were not in his favor, while these methods were often used when writing children's songs. This finding could be viewed as a character of his songwriting.

Modeling of Vegetation Phenology Using MODIS and ASOS Data (MODIS와 ASOS 자료를 이용한 식물계절 모델링)

  • Kim, Geunah;Youn, Youjeong;Kang, Jonggu;Choi, Soyeon;Park, Ganghyun;Chun, Junghwa;Jang, Keunchang;Won, Myoungsoo;Lee, Yangwon
    • Korean Journal of Remote Sensing
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    • v.38 no.5_1
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    • pp.627-646
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    • 2022
  • Recently, the seriousness of climate change-related problems caused by global warming is growing, and the average temperature is also rising. As a result, it is affecting the environment in which various temperature-sensitive creatures and creatures live, and changes in the ecosystem are also being detected. Seasons are one of the important factors influencing the types, distribution, and growth characteristics of creatures living in the area. Among the most popular and easily recognized plant seasonal phenomena among the indicators of the climate change impact evaluation, the blooming day of flower and the peak day of autumn leaves were modeled. The types of plants used in the modeling were forsythia and cherry trees, which can be seen as representative plants of spring, and maple and ginkgo, which can be seen as representative plants of autumn. Weather data used to perform modeling were temperature, precipitation, and solar radiation observed through the ASOS Observatory of the Korea Meteorological Administration. As satellite data, MODIS NDVI was used for modeling, and it has a correlation coefficient of about -0.2 for the flowering date and 0.3 for the autumn leaves peak date. As the model used, the model was established using multiple regression models, which are linear models, and Random Forest, which are nonlinear models. In addition, the predicted values estimated by each model were expressed as isopleth maps using spatial interpolation techniques to express the trend of plant seasonal changes from 2003 to 2020. It is believed that using NDVI with high spatio-temporal resolution in the future will increase the accuracy of plant phenology modeling.

Exploring the contextual factors of episodic memory: dissociating distinct social, behavioral, and intentional episodic encoding from spatio-temporal contexts based on medial temporal lobe-cortical networks (일화기억을 구성하는 맥락 요소에 대한 탐구: 시공간적 맥락과 구분되는 사회적, 행동적, 의도적 맥락의 내측두엽-대뇌피질 네트워크 특징을 중심으로)

  • Park, Jonghyun;Nah, Yoonjin;Yu, Sumin;Lee, Seung-Koo;Han, Sanghoon
    • Korean Journal of Cognitive Science
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    • v.33 no.2
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    • pp.109-133
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    • 2022
  • Episodic memory consists of a core event and the associated contexts. Although the role of the hippocampus and its neighboring regions in contextual representations during encoding has become increasingly evident, it remains unclear how these regions handle various context-specific information other than spatio-temporal contexts. Using high-resolution functional MRI, we explored the patterns of the medial temporal lobe (MTL) and cortical regions' involvement during the encoding of various types of contextual information (i.e., journalism principle 5W1H): "Who did it?," "Why did it happen?," "What happened?," "When did it happen?," "Where did it happen?," and "How did it happen?" Participants answered six different contextual questions while looking at simple experimental events consisting of two faces with one object on the screen. The MTL was divided to sub-regions by hierarchical clustering from resting-state data. General linear model analyses revealed a stronger activation of MTL sub-regions, the prefrontal lobe (PFC), and the inferior parietal lobule (IPL) during social (Who), behavioral (How), and intentional (Why) contextual processing when compared with spatio-temporal (Where/When) contextual processing. To further investigate the functional networks involved in contextual encoding dissociation, a multivariate pattern analysis was conducted with features selected as the task-based connectivity links between the hippocampal subfields and PFC/IPL. Each social, behavioral, and intentional contextual processing was individually and successfully classified from spatio-temporal contextual processing, respectively. Thus, specific contexts in episodic memory, namely social, behavior, and intention, involve distinct functional connectivity patterns that are distinct from those for spatio-temporal contextual memory.

Competitive Response of Rice Cultivar in Association with Plant Spacing and Seedling Number per Hill (수도의 주내 및 주간 경쟁반응에 관한 연구)

  • Park, Seong-Tae;Kim, Soon-Chul;Choi, Choong-Don;Lee, Soo-Kwan
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.30 no.3
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    • pp.252-258
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    • 1985
  • An experiment was conducted at the Yeongnam Crop Experiment Station to obtain basic informations about cultural techniques for high yielding by manipulating plant spacing using two rice cultivars, Samgangbyeo (Indica/Japonica type) and Nakdongbyeo (Japonica type), and four plant spacings, 10${\times}$10cm, 20${\times}$20cm 30${\times}$30cm and 40${\times}$40cm, with 4 kinds of seedling number per hill, 1,3,5 and 7, respectively. High photosynthetic efficiency (Eu) exhibited at the Samgangbyeo compared to Nakdongbyeo regardless of plant spacings and seedling numbers. For Samgangbyeo, Eu value was the highest at the 20${\times}$20cm plant spacing and five seedlings and seven seedlings per hill showed high Eu values at 10${\times}$10cm plant spacing and 20${\times}$20cm plant spacing, respectively, while other plant spacings were not significantly differed among seedling numbers. For Nakdongbyeo, however, one seedling plot obtained high Eu value at the 10${\times}$10cm plant spacing while this Eu value increased as the seedling number per hill increased in other plant spacings. There was a high positive correlation between rice grain yield and total competition index for both cultivars while kind of relationships differed in these two cultivars; linear relationship for Samgangbyeo and exponential relationship for Nakdongbyeo, respectively. Competition index between rice hill was more significant than within rice hill for Samgangbyeo while both competition indexs were important for Nakdongbyeo to increase rice yield.

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Analysis of Predicted Reduction Characteristics of Ash Deposition Using Kaolin as a Additive During Pulverized Biomass Combustion and Co-firing with Coal (미분탄 연소 시스템에 바이오매스 혼소시 카올린 첨가제 적용에 따른 회 점착 저감 특성 예측 연구)

  • Jiseon Park;Jaewook Lee;Yongwoon Lee;Youngjae Lee;Won Yang;Taeyoung Chae;Jaekwan Kim
    • Clean Technology
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    • v.29 no.3
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    • pp.193-199
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    • 2023
  • Biomass has been used to secure renewable energy certificates (REC) in domestic and overseas coal-fired power plants. In recent years, biofuel has been diversified from traditional wood pellets to non-woody biomass. Non-woody biomass has a higher content of alkaline metals such as K and Na than wood-based biomass, resulting in a lower melting point and an increase in slagging on boiler tubes, which reduces boiler efficiency. This study analyzed the effect of kaolin, an additive commonly used to increase melting points, on biomass co-firing to coal through thermochemical equilibrium calculations. In a previous experiment on biomass co-firing to coal conducted at 80 kWth, it was interpreted that the use of kaolin actually increased the amount of fouling. In this study, analysis showed that when kaolin was added, aluminosilicate compounds were generated due to Al2O3, which is abundant in coal, and mullite was formed. Thus, it was confirmed that the amount of slag increased when more kaolin was used. Further analysis was conducted by increasing the biomass co-firing rate from 0% to 100% at 10% intervals, and the results showed non-linear liquid slag generation. As a result, it was found that the least amount of liquid slag was generated when the biomass co-firing rate was between 50 and 60%. The phase diagram analysis showed that high melting point compounds such as leucite and feldspar were most abundantly generated under these conditions.

A study on solar radiation prediction using medium-range weather forecasts (중기예보를 이용한 태양광 일사량 예측 연구)

  • Sujin Park;Hyojeoung Kim;Sahm Kim
    • The Korean Journal of Applied Statistics
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    • v.36 no.1
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    • pp.49-62
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    • 2023
  • Solar energy, which is rapidly increasing in proportion, is being continuously developed and invested. As the installation of new and renewable energy policy green new deal and home solar panels increases, the supply of solar energy in Korea is gradually expanding, and research on accurate demand prediction of power generation is actively underway. In addition, the importance of solar radiation prediction was identified in that solar radiation prediction is acting as a factor that most influences power generation demand prediction. In addition, this study can confirm the biggest difference in that it attempted to predict solar radiation using medium-term forecast weather data not used in previous studies. In this paper, we combined the multi-linear regression model, KNN, random fores, and SVR model and the clustering technique, K-means, to predict solar radiation by hour, by calculating the probability density function for each cluster. Before using medium-term forecast data, mean absolute error (MAE) and root mean squared error (RMSE) were used as indicators to compare model prediction results. The data were converted into daily data according to the medium-term forecast data format from March 1, 2017 to February 28, 2022. As a result of comparing the predictive performance of the model, the method showed the best performance by predicting daily solar radiation with random forest, classifying dates with similar climate factors, and calculating the probability density function of solar radiation by cluster. In addition, when the prediction results were checked after fitting the model to the medium-term forecast data using this methodology, it was confirmed that the prediction error increased by date. This seems to be due to a prediction error in the mid-term forecast weather data. In future studies, among the weather factors that can be used in the mid-term forecast data, studies that add exogenous variables such as precipitation or apply time series clustering techniques should be conducted.

Effect of Fractionated X-ray Irradiation on Sprouted Barley Growth and Chlorophyll Concentration (X선의 분할조사가 새싹보리 생장과 클로로필 농도에 미치는 영향)

  • In Suck Park;Won-Jeong Lee;Sang-Bok Jeong
    • Journal of the Korean Society of Radiology
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    • v.17 no.7
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    • pp.1171-1178
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    • 2023
  • In study, we investigated changes on growth and chlorophyll concentration on sprouted barley by fractionated X-ray irradiation (FXI). Group was divided into the control group (CG), 1-time irradiation group (30 Gy once), 2-time irradiation group (15 Gy 2 times), and 3-time irradiation group (10 Gy 3 times), and 20 grains were used per group. Experimental group (EG) was exposed by using linear accelerator (Clinac IS, VERIAN, USA), by 6 MV X-ray, SSD 100 cm, 18×10 cm2, 600 MU/min. Length was measured every day until 9th day, and chlorophyl was analyzed using spectrophotometer(uv-1800, shimadzu, japan) in 9th day. Data analysis was performed the One-way ANOVA using SPSS ver 26.0(Chicago, IL, USA). In the pre-germination irradiation group (Pre-GIG), the CG had greater length than the EG on all measurement days, and as the number of FXI increased, the length became shorter. In the post-germination irradiation group (Post-GIG), the length of the CG was statistically significantly greater than that of the EG on all measurement days, and as the number of FXI increased, the length also became longer. The chlorophyll concentration was higher in the Post-GIG than in the Pre-GIG, and chlorophyll concentrations of EG was higher in the Pre-GIG than in the CG, as well as and Post-GIG. In addition, the smaller the number of FXI, the higher the chlorophyll concentration in both groups. FXI was found to affect the growth and chlorophyll concentration of sprouted barley.