• Journal of Oral Medicine and Pain
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• 제26권1호
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• pp.39-50
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• 2001

In order to investigate the effect of Transforming growth factor ${\beta}1$(below TGF-${\beta}1$) and osteogenic protein-1(below Op-1) onto the myogenic differentiation, C2C12 satellite myoblastic cell line was cultured and treated with both growth factors. At first morphological changes with microscopical examination were examined, and isolated total RNA to analyse mRNA expression of bone marker proteins, muscle regulatory proteins, TGF-${\beta}$ receptor and their ligands by Northern blot analysis. And cellular proliferative inducibility of both growth factors was also tested to C2C12 cells. Incubating the cell with $5ng/m{\ell}$ of TGF-${\beta}1$ until 4 days almost inhibited multinucleated myotube formation expressing muscular regulatory proteins, and induced decreasing Id proteins. However, no osteoblastic phenotypes was induced by TGF-${\beta}1$ in C2C12 cells. The mRNA expression of TGF-${\beta}$ receptors with TGF-${\beta}1$ was conversed after 48 hours cultured. Type I TGF-${\beta}$ receptor was seemed to play a role in negative signalling for inhibition of myogenic differentiation. OP-1 dose dependently induced ALP activity, osteopontine production and bone sialoprotein production at concentrations above $100ng/m{\ell}$ and osteocalcin production at concentrations above $300ng/m{\ell}$. The concentration of OP-1 required to induce these osteoblastic phenotypes was the same as that required to almost completely inhibit myotube formation. Incubation with above $100ng/m{\ell}$ OP-1 suppressed the expression of mRNA for muscular egulatory proteins from 2 days after incubation. Expression of Id-1, 2, 3 mRNA were stimulated by OP-1 at concentration above $300ng/m{\ell}$. When C2C12 cells were treated with both growth factors, TGF-${\beta}1$ potentiated the inhibitory effect of OP-1 on myotube formation and expression of mRNA for myogenin at 12 days. And TGF-${\beta}1$ reduced osteocalcin and bone sialoprotein production induced by OP-1 at 12 days in C2C12 cells. Both growth factor had no mitogenic effect. These results indicate that OP-1 converts the differentiation pathway of C2C12 myoblasts into that of osteoblastic lineage cells and it's not heritable, but TGF-${\beta}1$ does not and has reversible inhibitory activity on the myogenic differentiation. TGF-${\beta}1$ and OP-1 play a role in myogenic differentiation via different mechanism between them.

• Clinical and Experimental Reproductive Medicine
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• 제2권2호
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• pp.13-26
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• 1975

Seven aspects of attitude toward marriage in Korea are examined to better understand present and future marriage patterns. Also, various facets of current marriage practice are compared with attitudes. The study comprises three groups of roughly 600 women each, selected by random sampling from a rural, an urban, and a semi-urban area. A carefully designed and pretested questionnaire was checked for reliability by a reinterview in a 15% subsample. The great majority of Korean women support traditional attitudes that one must or should marry. The small group who recommend that one should not marry are mostly the very young or the never married, whose attitudes still may change. However, there are important and probably predictive shifts in favor of more individual decision, especially among the better educated, the young, and the more urban. Traditional reasons for marriage such as "custom" and procreation are ranked first by a majority, but there is a large shift to more contemporary or liberal desire for companionship and love, also primarily among the better educated, the urban, the young, and the never married. The traditional attitude that parents should have the sole or major role in mate selection is still held by a bare majority; the educated, urban, young, and never married are more liberal. Only 6% opt for each of the two extremes: That the parent alone or the respondent alone should decide. The remainder prefer one of the two middle-of-the-road positions where parent and child together decide. The proportions of respondents who classed specified criteria as moat important for selecting a husband, arranging the criteria in order from traditional to contemporary were: Lineage, etc., 23%; personal attributes, 40%; health and education, 27%; and love, 10%. The changing attitudes are suggested by the fact that love was ranked first by only 3% of the poorly educated rural poulation versus 23% of urban college level and 31% of the urban never married. There has been a substantial rise in the ideal age of marriage over the past twelve or more years, but there also is evidence that the ideal age is at or near a ceiling. Knowledge about legal age of marriage is minimal; the implications of this for proposed legislation are discussed. Three-fifthes to four-fifths of all respondents married husbands of the same religious, residential, and economic backgrounds as themselves. Almost all of them married men of the same or higher educational level. These evidences of traditional influences in mate selection are contrasted with the low priority given some of those items in earlier questions on reasons for marriage and criterion for selecting husband. Contrary to the expressed attitudes as to who should select the husband, we find that marriages of the study sample were stated to be arranged by parents alone in 62%; and in another 23%, the parents made the decision but asked the respondent's views. Such arrangements were most frequent among the rural, the less educated, and the older respondents and less common in the urban and more educated. The implications of these and related findings are discussed.

• Animal cells and systems
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• 제12권4호
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• pp.269-277
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• 2008

Siberian flying squirrel, an endangered species in South Korea, is distributed through major mountain regions of South Korea. The number of Siberian flying squirrel(Pteromys volans) in South Korea has decreased and their habitats are fragmented and isolated because of anthropogenic activities. So far no molecular genetic data has, however, been available for their conservation and management. To obtain better information concerning genetic diversity and phylogenetic relationships of the Siberian flying squirrel in South Korea, we examined 14 individuals from South Korea, 7 individuals from Russia, and 5 individuals from northeastern China along with previously published 29 haplotypes for 1,140 bp of the mtDNA cytochrome b gene. The 14 new individuals from South Korea had 7 haplotypes which were not observed in the regions of Russia and Hokkaido. The level of genetic diversity(0.616%) in the South Korean population was lower than that in eastern Russia(0.950%). The geographical distribution of mtDNA haplotypes and reduced median network confirmed that there are three major lineages of Siberian flying squirrel, occupying; Far Eastern, northern Eurasia, and the island of Hokkaido. The South Korean population only slightly distinct from the Eurasia, and eastern Russian population, and is part of the lineage Far Eastern. Based on these, we suggest that the South Korean population could be considered to belong to one partial ESU(Far Eastern) of three partial ESUs but a different management unit. However, the conservation priorities should be reconfirmed by nuclear genetic marker and ecological data.

• 한국응용곤충학회지
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• 제55권4호
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• pp.459-464
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• 2016

전라남도 해남군 산이면에 있는 간척지 중 친환경 사료작물 재배지에서 2014년도 8월에 풀무치(Locusta migratoria)가 대발생을 하여, 식량작물과 사료작물에 커다란 피해를 주었다. 오랜 기간 작물에 피해를 주지 않던 migratory locust가 어떠한 원인들에 의하여 대발생하여 해충화 되었는지에 아직까지 모르는 실정이다. 대발생의 원인을 규명하기 위한 연구의 일환으로 해남군 산이면에서 발생한 풀무치의 마이토콘드리아에 존재하는 16S ribosomal RNA와 D-loop의 염기서열을 분석하여 이들과 다른 지역계통간의 유전적인 연관성을 분석하였다. 그 결과 해남군 산이면에서 대량 발생한 풀무치는 북방계통 중 유라시아 대륙지역형과 유전적으로 가장 가까운 것으로 나타났다. 앞으로 2개의 표적 유전자는 국내외 유전적 집단 구조 비교에 활용할 수 있으며 대발생 풀무치의 대발생 원인을 분석하는데 기여할 수 있을 것이다.

• Journal of Periodontal and Implant Science
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• 제38권2호
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• pp.125-134
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• 2008

Purpose: Bone morphogenetic protein (BMP) is a potent differentiating agent for cells of the osteoblastic lineage. It has been used in the oral cavity under a variety of indications and with different carriers. However, the optimal carrier for each indication is not known. This study evaluated the bone regenerative effect of rhBMP-2 delivered with different carrier systems. Materials and Methods: 8 mm critical-sized rat calvarial defects were used in 60 male Sprague-Dawley rats. The animals were divided into 6 groups containing 10 animals each. Two groups were controls that had no treatment and absorbable collagen membrane only. 4 groups were experimentals that contained rhBMP-2 only and applied with absorbable collagen sponge($Collatape^{(R)}$), $MBCP^{(R)}$, Bio-$Oss^{(R)}$ each. The histological and histometric parameters were used to evaluate the defects after 2- or 8-week healing period. The shape and total augmented area were stable in all groups over the healing time. Results: New bone formation was significantly greater in the rhBMP-2 with carrier group than control group. rhBMP-2/ACS was the highest in bone density but gained less new bone area than rhBMP-2/$MBCP^{(R)}$ and rhBMP-2/Bio-$Oss^{(R)}$. The bone density after 8 weeks was greater than that after 2 weeks in all groups. However, rhBMP-2 alone failed to show the statistically significant difference in new bone area and bone density compared to control group. Also $MBCP^{(R)}$ and Bio-$Oss^{(R)}$ particles remained after 8 weeks healing period. Conclusion: These results suggest that rhBMP-2 with carrier system is an excellent inductive agent for bone formation and we can use it as the predictable bone tissue engieering technique. Future study will likely focus on the kinetics of BMP release and development of carriers that is ideal for it.

• Tuberculosis and Respiratory Diseases
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• 제44권6호
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• pp.1390-1395
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• 1997

혈관내 림프종증(intravascular lymphomatosis, IVL)은 1959년 처음 보고되었으며, 주로 B 림프구의 혈관 내부에 국한된 악성 증식으로 발생하는 드문 악성 질환이다. IVL의 주된 침범 부위는 중추신경계이며 그 외 피부, 심장, 폐, 간 등 전신장기를 침범할 수 있다. IVL의 폐침범은 드문 경우로 주된 증상은 호흡곤란, 발열, 기침 등이며 방사선학적으로 간질성 침윤으로 나타난다. 최근 저자 등은 고열과 흉부 통증을 동반하여 발생한 폐를 침범한 혈관내 림프종증 1예를 경험하였기에 단순 흉부촬영에서 간질성 침윤을 동반하는 원인 미상의 발열과 호흡곤란의 드문 감별 진단의 하나로 이를 보고한다.

• 생명과학회지
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• 제26권6호
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• pp.711-720
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• 2016

게놈분석이 완료된 680개의 세균의 공통 유전자 보유 정도와 유연관계를 파악하기 위해 4,631개의 COG (Clusters of Orthologous Groups of protein) 보유 유사도와 COG 보유 계통수를 작성하여 다음과 같은 결과를 얻었다. 균주별 COG 보유개수는 103~2,199개 사이였고 평균 1377.1개 였다. 곤충과 절대공생성인 Candidatus Nasuia deltocephalinicola str. NAS-ALF가 최저였고 기회성병원균인 Pseudomonas aeruginosa PAO1가 최대였다. 2개의 세균들 사이에 나타내는 COG 보유 유무의 유사도는 49.30~99.78% 사이였고 평균 72.65%였다. 초고온성이며 자가영양생활을 하는 Methanocaldococcus jannaschii DSM 2661과 중온성이며 공생생활을 하는 Mesorhizobium loti MAFF303099 사이가 최소였다. 유전자 보유 정도가 생물이 각 서식지에 적응하는 정도를 나타내므로 이 결과는 원핵생물 진화의 역사 혹은 현재 지구의 원핵생물 서식지 범위를 나타내는 것일 수도 있다. COG 보유계통수를 통하여 첫째 진정세균인 Chloroflexi문의 일부는 진정세균보다 고세균과 유연관계가 높았고, 둘째 16S rRNA유전자에서 동일한 문(phylum)이나 강(class)으로 분류되지만 COG 보유 계통수에서는 일치하지 않는 경우가 많았으며, 셋째 delta-와 epsilon-Proteobacteria는 다른 Proteobacteria와 다른 분계(lineage)를 이루었다. 본 연구결과는 생물의 기원 파악과 기능적 연관성 파악 그리고 유용유전자 탐색 등에 이용할 수 있을 것이다.

• Molecules and Cells
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• 제41권6호
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• pp.582-590
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• 2018

Endothelial progenitor cells (EPCs) and outgrowth endothelial cells (OECs) play a pivotal role in vascular regeneration in ischemic tissues; however, their therapeutic application in clinical settings is limited due to the low quality and quantity of patient-derived circulating EPCs. To solve this problem, we evaluated whether three priming small molecules (tauroursodeoxycholic acid, fucoidan, and oleuropein) could enhance the angiogenic potential of EPCs. Such enhancement would promote the cellular bioactivities and help to develop functionally improved EPC therapeutics for ischemic diseases by accelerating the priming effect of the defined physiological molecules. We found that preconditioning of each of the three small molecules significantly induced the differentiation potential of $CD34^+$ stem cells into EPC lineage cells. Notably, long-term priming of OECs with the three chemical cocktail (OEC-3C) increased the proliferation potential of EPCs via ERK activation. The migration, invasion, and tube-forming capacities were also significantly enhanced in OEC-3Cs compared with unprimed OECs. Further, the cell survival ratio was dramatically increased in OEC-3Cs against $H_2O_2$-induced oxidative stress via the augmented expression of Bcl-2, a pro-survival protein. In conclusion, we identified three small molecules for enhancing the bioactivities of ex vivo-expanded OECs for vascular repair. Long-term 3C priming might be a promising methodology for EPC-based therapy against ischemic diseases.

• 대한임상검사과학회지
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• 제41권4호
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• pp.145-152
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• 2009

Novel influenza A virus, subtype H1N1 of swine-lineage, has been transmitted rapidly to many regions of the world. Rapid detection of the virus is essential to instigate appropriate patient care and public health management and for disease surveillance. The aim of this study is to determine the prevalence of novel influenza A (H1N1) virus in Korea using reverse-transcription real time polymerase chain reaction (rRT-PCR). Novel H1N1 virus was detected in a total of 8,948 nasopharyngeal samples from patients with influenza-like illness throughout Korea from August to September 2009. RNA was extracted from $300{\mu}l$of sample using an RNA extraction kit (Zymo Research, CA, USA). In the present study, Genekam kit (Genekam, Duisburg, Germany) was used to detect novel H1N1 virus. Novel H1N1 virus was found in 1,130 samples from a total of 8,948 samples (12.6%). The highest frequency was found in 10- to 19-year-olds (M: 29.3% vs. F: 16.4%), followed by 20- to 29-year-olds (M: 17.9% vs. F: 15.4%), 40- to 49-year-olds (M: 6.5% vs. F: 8.1%), 50- to 59-year-olds (M: 6.0% vs. F: 5.5%), and 30- to 39-year-olds (M: 4.6% vs. F: 3.8%). The mean positive rate was higher in men than in women (M: 14.7% vs. F: 7.4%). Novel H1N1 virus showed the lowest prevalence in patients over 60 years old. The positive rate increased daily and showed a significant high peak in mid-September 2009. In 19 provinces of Korea, Cheonan (41.1%), Busan (37.3%), Gangneung (33.3%), Jinju (32.1%), Ulsan (24.6%), Deajeon (23.7%) areas showed high frequencies and other provinces were found less than 10% of novel H1N1 virus. Since reverse-transcription real time PCR assay is rapid, accurate, and convenient, it may assist public health laboratories in detecting novel H1N1 virus. Moreover, these data could be useful for the management of patients with influenza-like illness.

• 미생물학회지
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• 제45권4호
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• pp.368-376
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• 2009

동중국해 해양퇴적층에서 다량의 적색색소를 생산하는 해양미생물을 분리하였다. 분리균주의 분류학적 특성의 확인을 위해 형태학적, 생리학적, 생화학적 특성, 세포지방산 분석, 16S rDNA 염기서열을 이용한 분자 계통학적 분석을 통해 분류학적 위치를 탐색하였다. 분자 계통학적 분석 결과 Zooshikella 속에 포함되는 것으로 확인되었고 Zooshikella ganghwensis KCTC $12044^T$ (AY130994)와 99.79%의 가장 높은 유사도를 보였다. 분리균주는 그람음성의 간균으로 호기성 및 NaCl 의존적인 배양특성을 보였다. 세포 지방산 분석결과 type strain과 주요 지방산 조성이 유사하였으며 이러한 특성을 종합하여 Zooshikella sp. JE-34로 동정하였다. JE-34가 생산하는 적색색소는 이미 보고되어 널리 알려진 Serratia marcescens에서 생산되는 적색색소인 prodigiosin의 특성과 유사하였다. 색소물질의 인체병원균 및 어류질병 유발세균에 대한 항균활성을 검토한 결과 18종의 피검균 중 특히 Streptococcus iniae, S. parauberis, S. mutans, Staphylococcus aureus, Propionibacterium acnes에 대한 항균활성이 우수하였다.