Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

Effects of rhBMP-2 with various carriers on bone regeneration in rat calvarial defect

백서 두개골 결손에서 rhBMP-2와 다양한 carrier의 골재생 유도효과

  • Lee, Seo-Kyoung (Department of Periodontics, National Health Insurance Corporation Ilsan Hospital) ;
  • Kim, Ji-Sun (Department of Periodontics, National Health Insurance Corporation Ilsan Hospital) ;
  • Kang, Eun-Jung (Osstem Co.) ;
  • Eum, Tae-Kwan (Osstem Co.) ;
  • Kim, Chang-Sung (Department of Periodontology, Yonsei University College of Dentistry) ;
  • Cho, Kyoo-Sung (Department of Periodontology, Yonsei University College of Dentistry) ;
  • Chai, Jung-Kiu (Department of Periodontology, Yonsei University College of Dentistry) ;
  • Kim, Chong-Kwan (Department of Periodontology, Yonsei University College of Dentistry) ;
  • Choi, Seong-Ho (Department of Periodontology, Yonsei University College of Dentistry)
  • 이서경 (국민건강보험공단 일산병원 치주과) ;
  • 김지선 (국민건강보험공단 일산병원 치주과) ;
  • 강은정 ((주)오스템) ;
  • 엄태관 ((주)오스템) ;
  • 김창성 (연세대학교 치과대학 치주과학교실) ;
  • 조규성 (연세대학교 치과대학 치주과학교실) ;
  • 채중규 (연세대학교 치과대학 치주과학교실) ;
  • 김종관 (연세대학교 치과대학 치주과학교실) ;
  • 최성호 (연세대학교 치과대학 치주과학교실)
  • Published : 2008.06.30
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Abstract

Purpose: Bone morphogenetic protein (BMP) is a potent differentiating agent for cells of the osteoblastic lineage. It has been used in the oral cavity under a variety of indications and with different carriers. However, the optimal carrier for each indication is not known. This study evaluated the bone regenerative effect of rhBMP-2 delivered with different carrier systems. Materials and Methods: 8 mm critical-sized rat calvarial defects were used in 60 male Sprague-Dawley rats. The animals were divided into 6 groups containing 10 animals each. Two groups were controls that had no treatment and absorbable collagen membrane only. 4 groups were experimentals that contained rhBMP-2 only and applied with absorbable collagen sponge($Collatape^{(R)}$), $MBCP^{(R)}$, Bio-$Oss^{(R)}$ each. The histological and histometric parameters were used to evaluate the defects after 2- or 8-week healing period. The shape and total augmented area were stable in all groups over the healing time. Results: New bone formation was significantly greater in the rhBMP-2 with carrier group than control group. rhBMP-2/ACS was the highest in bone density but gained less new bone area than rhBMP-2/$MBCP^{(R)}$ and rhBMP-2/Bio-$Oss^{(R)}$. The bone density after 8 weeks was greater than that after 2 weeks in all groups. However, rhBMP-2 alone failed to show the statistically significant difference in new bone area and bone density compared to control group. Also $MBCP^{(R)}$ and Bio-$Oss^{(R)}$ particles remained after 8 weeks healing period. Conclusion: These results suggest that rhBMP-2 with carrier system is an excellent inductive agent for bone formation and we can use it as the predictable bone tissue engieering technique. Future study will likely focus on the kinetics of BMP release and development of carriers that is ideal for it.

Keywords

References

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