• 대한후두음성언어의학회지
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• 제5권1호
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• pp.44-58
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• 1994

The formants of the 9 Korean standard vowels(which used by the average people of Seoul, central-area of the Korean peninsula) were measured by analysis with the linear predictive coding(LPC) and fast Fourier transform(FFT). The author already had reported the constriction area for the Korean standard vowels, and with the existing data, the distance from glottis to the constriction area in the vocal tract of each vowel was newly measured with videovelopharyngograms and lateral Rontgenograms of the vocal tract. We correlated the formant frequencies with the distance from glottis to the constriction area of the vocal tract. Also we tried to correlate the formant frequencies with the position of tongue in the vocal tract which is divided into 2 categories : The position of tongue in oral cavity by the distance from imaginary palatal line to the highest point of tongue and the position in pharyngeal cavity by the distance from back of tongue to posterior pharyngeal wall. This study was performed with 10 adults(male : 5, female : 5) who spoke primary 9 Korean standard vowels. We had already reported that the Korean vowel [i], [e], $[{\varepsilon}]$ were articulated at hard palate level, [$\dot{+}$], [u] were at soft palate level, [$\wedge$] was at upper pharynx level and the [$\wedge$], [$\partial$], [a] in a previous article. Also we had noted that the significance of pharyngeal cavity in vowel articulation. From this study we have concluded that ; 1) The F$_1$ is related with the oral cavity articulated vowel [i, e, $\varepsilon$, $\dot{+}$, u]. 2) Within the oral cavity articulated vowel [i, e, $\varepsilon$, $\dot{+}$, u] and the upper pharynx articulated vowel [o], the F$_2$ is elevated when the diatance from glottis to the constriction area is longer. But within the lower pharynx articulated vowel [$\partial$, $\wedge$, a], the F$_2$ is elevated when the distance from glottis to the constriction area is shorter. 3) With the stronger tendency of back-vowel, the higher the elevation of the F$_1$ and F$_2$ frequencies. 4) The F$_3$ and F$_4$ showed no correaltion with the constriction area nor the position of tongue in the vocal tract 5) The parameter F$_2$- F$_1$, which is the difference between F$_2$ frequency and F$_1$ frequency showed an excellent indicator of differenciating the oral cavity articulated vowels from pharyngeal cavity articulated vowels. If the F$_2$-F$_1$ is less than about 600Hz which indicates the vowel is articulated in the pharyngeal cavity, and more than about 600Hz, which indicates that the vowel is articulated in the oral cavity.

• Asian-Australasian Journal of Animal Sciences
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• 제25권5호
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• pp.621-628
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• 2012

The FAT-1 protein is an n-3 fatty acid desaturase, which can recognize a range of 18- and 20-carbon n-6 substrates and transform n-6 polyunsaturated fatty acids (PUFAs) into n-3 PUFAs while n-3 PUFAs have beneficial effect on human health. Fat1 gene is the coding sequence from Caenorhabditis elegans which might play an important role on lipometabolism. To reveal the function of fat1 gene in bovine fetal fibroblast cells and gain the best cell nuclear donor for transgenic bovines, the codon of fat1 sequence was optimized based on the codon usage frequency preference of bovine muscle protein, and directionally cloned into the eukaryotic expression vector pEF-GFP. After identifying by restrictive enzyme digests with AatII/XbaI and sequencing, the fusion plasmid pEF-GFP-fat1 was identified successfully. The pEF-GFP-fat1 vector was transfected into bovine fetal fibroblast cells mediated by Lipofectamine2000$^{TM}$. The positive bovine fetal fibroblast cells were selected by G418 and detected by RT-PCR. The results showed that a 1,234 bp transcription was amplified by reverse transcription PCR and the positive transgenic fat1 cell line was successfully established. Then the expression level of fat1 gene in positive cells was detected using quantitative PCR, and the catalysis efficiency was detected by gas chromatography. The results demonstrated that the catalysis efficiency of fat1 was significantly high, which can improve the total PUFAs rich in EPA, DHA and DPA. Construction and expression of pEF-GFP-fat1 vector should be helpful for further understanding the mechanism of regulation of fat1 in vitro. It could also be the first step in the production of fat1 transgenic cattle.

• BMB Reports
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• 제31권6호
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• pp.578-584
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• 1998

In a previous paper (Kim et al., 1996a), the immediate 5' -flanking region and coding region of the human UDP-N -acetylglucosamine:-D-mannoside-1,4-Nacetylglucosaminyltransferase III (N-acetylglucosaminyitransferase- III; GnT-III) gene was reported, isolated and analyzed. Herein, we report on amplification of a new 5' -noncoding region of the GnT-III mRNA by single-strand ligation to single-stranded cDNA-PCR (5' -RACE PCR) using poly(A)+ RNA isolated from human fetal liver cells. A cDNA clone was obtained with 5' sequences (96 bp) that diverged seven nucleotides upstream from the ATG (+1) start codon. A concensus splice junction sequence, TCTCCCGCAG, was found immediately 5' to the position where the sequences of the cDNA diverged. The result suggested the presence of an intron in the 5' -noncoding region and that the cDNA was an incompletely reversetranscribed cDNA product derived from an mRNA containing a new noncoding exon. When mRNA expression of GnT-III in various human tissues and cancer cell lines was examined, Northern blot analysis indicated high expression levels of GnT-III in human fetal kidney and brain tissues, as well as for a number of leukemia and lymphoma cancer cell lines. Promoter activities of the 5' -flanking regions of exon 1 and the new noncoding region were measured in a human hepatoma cell line, HepG2, by luciferase assays. The 5'-flanking region of exon 1 was the most active, whilst that of exon 2 was inactive.

• 유통과학연구
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• 제13권5호
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• pp.83-90
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• 2015

Purpose - This research aims to understand the different behaviors of consumers according to cognitive age. Specifically, this research is focused on pro-social behavior. Pro-social behavior is defined as behavior benefiting others, rather than behavior benefiting one's self. It often entails individual risk or cost, such as when giving resources to others, waiting in line, asking for or paying a fair price, or risking one's life in battle. Therefore, we sought to understand consumer psychology and cognitive age as a reflection of inner psychology. People frequently perceive themselves as younger or older than their chronological ages. This self-perceived or cognitive age is a subjective age perception independent of actual chronological age. The discrepancy degree between chronological and cognitive age represents how much individuals perceive themselves as younger than they are. This study examines the gap in donation intention based on cognitive age. In order to investigate cognitive age, composed of four sub-categories (feel-age, look-age, do-age, and interest-age), this study explores the differential donation intention based on cognitive age, which determines the relationship between the young age and old age. Research design, data, and methodology - Data research was conducted by gathering 216 survey samples, excluding those with unreliable answers. Data coding and cleaning were used and SPSS 19.0 software for the data analysis. The respondents were categorized into two types, younger cognitive ages and older cognitive ages. Additionally, we analyzed the moderating variables. In particular, we used cognitive age degree and congruency level (cognitive age low vs. cognitive age high) × (congruency close vs. congruency distant) between - subjects design. First, regression was done to verify the difference between chronological age and cognitive age. Second, a t-test was done to verify the difference of cognitive age level in donations. Third, ANOVA (analysis of variance) was done to verify the difference between cognitive age and congruency in donations. Last, ANOVA was done to verify the difference between cognitive age and moral judgments in donations. Result - The results show most respondents perceive themselves as younger than their chronological ages. In particular, older respondents feel they are younger than their actual age. Moreover, the result of the comparison between low degree and high degree groups of cognitive age, show high donations at the higher degree of cognitive age groups. In addition, the closer the distance to the beneficiaries, the higher the donation in high degree cognitive age groups. The higher moral judgment groups also show relatively high contributions in lower degree cognitive age groups. Conclusions - Donations belong to the category of pro-social behavior reflecting an individual's psychological state. Therefore, it is important in understanding cognitive age. This study implies that it is necessary to take into account both cognitive age and chronological age when segmenting donors. Moreover, this study confirmed that there are different factors affecting the motives behind donations. Thus, it may be utilized to create differential donation strategies.

• 한국초등수학교육학회지
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• 제13권1호
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• pp.75-95
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• 2009

본 연구의 목적은 아동들의 수학문제해결 과정에서 나타나는 아동 간 스캐폴딩 과정을 분석하는 것이다. 연구의 참여자들은 3개 그룹 4학년 6명의 초등학생들을 대상으로 연구는 8월부터 12월까지 5개월간 심층적인 관찰과 인터뷰를 통하여 자료를 수집하여 분석하였다. 연구의 결과 첫째, 아동 간 스캐폴딩 과정에서는 교사와 같은 성인의 개입이 없는 경우 자유롭고 다양한 스캐폴딩의 형태가 관찰되었다. 둘째, 스캐폴딩 과정의 흐름은 조건, 현상, 작용/상호작용, 결과로 나타났다. 셋째, 인지적 도움은 정서적 도움이 순차적이고 독립적으로 일어나는 것이 아니라 통합적으로 일어났다. 교사들은 이 스캐폴딩의 과정에 대한 분석과 의미 도출로 학습자들의 보다 의미 있는 수학학습을 도울 수 있다.

• 공학교육연구
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• 제8권1호
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• pp.71-83
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• 2005

본 논문에서는 자바 언어를 학습하는데 있어 효율성을 증가시키기 위해 인터넷 기반 자율학습시스템이 제안되었다. 제안된 자율학습시스템은 JWP(Java Web Player)라고 불리며 Java Web Start 기술을 활용하여 웹상에서 실행이 가능한 자바 애플리케이션 프로그램이다. 또한 본 논문에서는 컴퓨터 언어를 학습하는데 있어 3가지 중요한 일련의 과정인 개념학습과정, 프로그래밍 실습과정, 그리고 학습 성취도 평가과정을 Java Web Start 기술을 이용하여 JWP에 통합하였다. 제안된 시스템은 학습과정을 교육공학적인 측면에서 멀티미디어 요소를 강화하였기 때문에 학습자가 흥미를 가지고 자발적으로 학습을 할 수 있도록 설계되었다. 더욱이 JWP 에는 효율적인 자바 언어 학습을 위해 학습내용에 대한 설명이 음성으로 출력되며, 이때 이와 관련된 이미지와 텍스트들이 동기화되어 동시에 화면에 표시된다. 더욱이 소스파일의 코딩, 에디팅, 실행 그리고 디버깅 등을 쉽게 할 수 있는 컴파일러가 삽입되어 있어 편리한 자바 언어 실습환경을 제공한다. 마지막으로 각 단원별 돌발퀴즈와 마무리 테스트를 통하여 학습자가 자신의 학습상황을 체크하여 반복학습을 할 수 있도록 유도하였다.

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2000년도 International Meeting 2000
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• pp.23-36
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• 2000

Japanese encephalitis virus (JEV) is the causative agent of a mosquito-borne encephalitis and is transmitted to human via persistently infected mosquito vectors. Although the virus is known to cause only acute infection, there were reports that showed neurological sequelae, latent infection in peripheral mononuclear cells, and recurrence of the disease after acute encephalitis. Innate resistance of certain cell lines, abnormal SN1 expression of the virus, and anti-apoptotic effect of cullular bcl-2 have been suggested as probable causes of JEV persistence even in the absence of defective interfering (DI) particles. Although possible involvement of DI particles in JEV persistence was suggested, neither has a direct evidence for DI presence nor its molecular characterization been made. Two questions asked in this study are whether the DI virus plays any role in JEV persistent infection if it is associated with and what type of change(s) can be made in persistently infected cells to avoid apoptosis even with the continuous virus replication, DI-free standard stock of JEV was infected in BHK-21, Vero, and SW13 cells and serial high multiplicity passages were performed in order to generate DI particles. There different-sized DI RNA species which were defective in both structural and nonstructural protein coding genes. Rescued ORFs of the DI genome maintained in-frame and the presence of replicative intermediate or replicative form RNA of the DI particles confirmed their replication competence. On the other hand, several clones with JEV persistent infection were established from the cells survived acute infections during the passages. Timing of the DI virus generation during the passages seemed coincide to the appearance of persistently infected cells. The DI RNAs were identified in most of persistently infected cells and were observed throughout the cell maintenance. One of the cloned cell line maintained the viral persistence without DI RNA coreplication. The cells with viral persistence released the reduced but continuous infectious JEV particle for up to 9 months and were refractory to homologous virus superinfection but not to heterologous challenges. Unlike the cells with acute infection these cells were devoid of characteristic DNA fragmentation and JEV-induced apoptosis with or without homologous superinfection. Therefore, the DI RNA generated during JEV undiluted serial passage on mammalian cells was shown to be biologically active and it seemed to be responsible, at least in part, for the establishment and maintenance of the JEV persistence in mammalian cells. Viral persistence without DI RNA coreplication, as in one of the cell clones, supports that JEV persistent infection could be maintained with or without the presence of DI particles. In addition, the fact that the cells with JEV persistence were resistant against homologous virus superinfection, but not against heterologous one, suggests that different viruses have their own and independent pathway for cytopathogenesis even if viral cytopathic effect could be converged to an apoptosis after all.

• 대한전자공학회논문지SP
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• 제49권1호
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• pp.19-30
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• 2012

디지털 신호의 양을 줄이기 위한 손실 소스 부호화에서 양자화는 필수적이다. 이때 보다 효율적인 양자화를 위해서는 벡터양자기(vector quantizer: VQ)를 사용하는데, 벡터의 차수 또는 전송률이 올라감에 따라 VQ의 부호화 복잡도는 기하급수적으로 증가한다. 이를 보완하기 위하여 여러 변형된 VQ가 제안되어 있다. 이러한 변형된 VQ의 일종으로 표본 적응 프로덕트 양자기(sample-adaptive product quantizer: SAPQ)가 있는데, 벡터의 차수를 줄여서 부호화 복잡도를 줄일 수 있는 프로덕트 VQ(product VQ: PQ)와 유사한 구조를 가지지만, 일반 PQ보다 더 좋은 성능을 가지면서 일반 VQ보다는 부호화 복잡도가 낮고 부호책을 위한 메모리의 크기도 작은 일종의 구조적 제한을 가지는 VQ이다. 이러한 SAPQ 중에서 부호책의 구조가 양자화 공간의 대각선에 대칭 형태를 가지는 단순한 형태의 1-SAPQ가 있는데, 이러한 1-SAPQ의 성능은 동일한 분포를 가지며 서로 독립인 입력에 좋은 성능을 보인다. 본 논문에서는 1-SAPQ를 1차 마르코프 과정에 대하여 설계하고 그 성능을 평가하였다. 효율적인 1-SAPQ의 설계를 위하여 초기 부호책 설계 알고리듬을 제안하였으며, 수치해석을 통하여 1-SAPQ는 비슷한 부호화 복잡도를 가지는 VQ보다 좋은 성능을 보임을 보였다. 또한 DPCM(differential pulse coded modulation) 기법에 Lloyd-Max 양자화를 사용한 경우의 성능에 근접함을 보였다.

• 한국실내디자인학회논문집
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• 제25권2호
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• pp.21-29
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• 2016

This study has estimated the traits of conscious eye moving with the objects of the halls of subway stations. For that estimation, the observation data from eye-tracking were matched with the experiment images, while an independent program was produced and utilized for the analysis of the eye moving in the selected sections, which could provide the ground for clarifying the traits of space-users' eye moving. The outcomes can be defines as the followings. First, The application of the independently produced program provides the method for coding the great amount of observation data, which cut down a lot of analysis time for finding out the traits of conscious eye moving. Accordingly, the inclusion of eye's intentionality in the method for extracting the characteristics of eye moving enabled the features of entrance and exit of particular objects with the course of observing time to be organized. Second, The examination of eye moving at each area surrounding the object factors showed that [out]${\rightarrow}$[in], which the line of sight is from the surround area to the objects, characteristically moved from the left-top (Area I) of the selected object to the object while [in]${\rightarrow}$[out], which is from the inside of the object to the outside, also moved to the left-top (Area I). Overall, there were much eye moving from the tops of right and left (Area I, II) to the object, but the eye moving to the outside was found to move to the left-top (Area I), the right-middle (Area IV) and the right-top (Area II). Third, In order to find if there was any intense eye-moving toward a particular factor, the dominant standards were presented for analysis, which showed that there was much eye-moving from the tops (Area I, II) to the sections of 1 and 2. While the eye-moving of [in] was [I $I{\rightarrow}A$](23.0%), [$I{\rightarrow}B$](16.1%) and [$II{\rightarrow}B$](13.8%), that of [out] was [$A{\rightarrow}I$](14.8%), [$B{\rightarrow}I$](13.6%), [$A{\rightarrow}II$](11.4%), [$B{\rightarrow}IV$](11.4%) and [$B{\rightarrow}II$](10.2%). Though the eye-moving toward objects took place in specific directions (areas), that (out) from the objects to the outside was found to be dispersed widely to different areas.

• 정보보호학회논문지
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• 제30권6호
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• pp.1131-1139
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• 2020

소스코드에 대한 정적 분석은 광범위한 소스코드에 대해서 잔존하는 보안약점을 찾는 것으로 정적 분석 도구를 활용하여 점검을 하고, 그 결과에 대해서 정적 분석 전문가가 정탐 및 오탐 분석을 한다. 이 과정에서 분석양이 많고 오탐의 비율이 높아 많은 시간과 노력이 들어가게 되어 효율적으로 분석하는 방안이 요구되고 있다. 또한 전문가들은 정·오탐 분석을 할 때 결함이 발생한 라인의 소스코드만 보고 분석을 하는 경우는 드물다. 결함의 유형에 따라서 주변의 소스코드를 같이 분석하고 최종 분석 결과를 내리게 된다. 이러한 정적 분석 도구를 사용하여 전문가가 정·오탐을 판별하는 어려움을 해결하기 위해서 본 논문에서는 정적 분석 도구가 찾은 보안약점이 정탐인지 아닌지를 전문가가 아닌 인공지능을 통해 판별하는 방법을 제안한다. 또한 이러한 기계학습에 사용되는 학습 데이터(결함주변 소스코드)의 크기가 성능에 어떤 영향을 미치는지 실험을 통해 최적의 크기를 확인하였다. 이 결과를 통해 정적 분석 후 정·오탐을 분류하는 정적 분석 전문가의 업무에 도움을 줄 것으로 기대한다.