Construction of fat1 Gene Expression Vector and Its Catalysis Efficiency in Bovine Fetal Fibroblast Cells
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Liu, Boyang
(College of Animal Science and Veterinary Medicine, and Jilin Provincial Key Laboratory of Animal Embryo Engineering, Jilin University)
Yang, Runjun (College of Animal Science and Veterinary Medicine, and Jilin Provincial Key Laboratory of Animal Embryo Engineering, Jilin University) Li, Junya (Institute of Animal Sciences, Chinese Academy of Agricultural Sciences) Zhang, Lupei (Institute of Animal Sciences, Chinese Academy of Agricultural Sciences) Liu, Jing (College of Animal Science and Veterinary Medicine, and Jilin Provincial Key Laboratory of Animal Embryo Engineering, Jilin University) Lu, Chunyan (College of Animal Science and Veterinary Medicine, and Jilin Provincial Key Laboratory of Animal Embryo Engineering, Jilin University) Lian, Chuanjiang (College of Animal Science and Veterinary Medicine, and Jilin Provincial Key Laboratory of Animal Embryo Engineering, Jilin University) Li, Zezhong (College of Animal Science and Veterinary Medicine, and Jilin Provincial Key Laboratory of Animal Embryo Engineering, Jilin University) Zhang, Yong-Hong (College of Animal Science and Veterinary Medicine, and Jilin Provincial Key Laboratory of Animal Embryo Engineering, Jilin University) Zhang, Liying (College of Animal Science and Veterinary Medicine, and Jilin Provincial Key Laboratory of Animal Embryo Engineering, Jilin University) Zhao, Zhihui (College of Animal Science and Veterinary Medicine, and Jilin Provincial Key Laboratory of Animal Embryo Engineering, Jilin University) |
1 | Li, D., A. Sinclair, A. Wilson, S. Nakkote, F. Kelly, L. Abedin, N. Mann and A. Turner. 1999. Effect of dietary alpha-linolenic acid on thrombotic risk factors in vegetarian men. Am. J. Clin. Nutr. 69:872-882. |
2 | Lopez-Huertas, E. 2010. Health effects of oleic acid and long chain omega-3 fatty acids (EPA and DHA) enriched milks. A review of intervention studies. Pharmacol. Res. 61:200-207. DOI ScienceOn |
3 | Lu, Y., D. Nie, W. T. Witt, Q. Chen, M. Shen, H. Xie, L. Lai, Y. Dai and J. Zhang. 2008. Expression of the fat-1 gene diminishes prostate cancer growth in vivo through enhancing apoptosis and inhibiting GSK-3 beta phosphorylation. Mol. Cancer Ther. 7:3203-3211. DOI ScienceOn |
4 | Marra, M. A., S. J. Jones, C. R. Astell, R. A. Holt, A. Brooks-Wilson, Y. S. Butterfield, J. Khattra, J. K. Asano, S. A. Barber and S. Y. Chan et al. 2003. The Genome sequence of the SARS-associated coronavirus. Science 300:1399-1404. DOI ScienceOn |
5 | Meesapyodsuk, D., D. W. Reed, C. K. Savile, P. H. Buist, S. J. Ambrose and P. S. Covello. 2000. Characterization of the regiochemistry and cryptoregiochemistry of a Caenorhabditis elegans fatty acid desaturase (FAT-1) expressed in Saccharomyces cerevisiae. Biochemistry 39:11948-11954. DOI ScienceOn |
6 | Moussavi, A. R., R. O. Gilbert, T. R. Overton, D. E. Bauman and W. R. Butler. 2007a. Effects of feeding fish meal and n-3 fatty acids on milk yield and metabolic responses in early lactating dairy cows. J. Dairy Sci. 90:136-144. DOI ScienceOn |
7 | Moussavi, A. R., R. O. Gilbert, T. R. Overton, D. E. Bauman and W. R. Butler. 2007b, Effects of feeding fish meal and n-3 fatty acids on ovarian and uterine responses in early lactating dairy cows. J. Dairy Sci. 90:145-154. DOI ScienceOn |
8 | Abbadi, A., F. Domergue, J. Bauer, J. A. Napier, R. Welti, U. Zahringer, P. Cirpus and E. Heinz. 2004. Biosynthesis of very-long-chain polyunsaturated fatty acids in transgenic oilseeds: constraints on their accumulation. Plant Cell 16:2734-2748. DOI ScienceOn |
9 | Bauer, A. P., D. Leikam, S. Krinner, F. Notka, C. Ludwig, G. Langst and R. Wagner. 2010. The impact of intragenic CpG content on gene expression. Nucleic Acids Res. 38:3891-3908. DOI |
10 | Connor, K. M., J. P. SanGiovanni, C. Lofqvist, C. M. Aderman, J. Chen, A. Higuchi, S. Hong, E. A. Pravda, S. Majchrzak and D. Carper et al. 2007. Increased dietary intake of omega-3-polyunsaturated fatty acids reduces pathological retinal angiogenesis. Nat. Med. 13:868-873. DOI ScienceOn |
11 | Enser, M., K. Hallett, B. Hewitt, G. A. J. Fursey and J. D. Wood. 1996. Fatty acid content and composition of english beef, lamb and pork at retail. Meat Sci. 42:443-456. DOI ScienceOn |
12 | Enser, M., K. G. Hallett, B. Hewitt, G. A. J. Fursey, J. D. Wood and G. Harrington. 1998, Fatty acid content and composition of UK beef and lamb muscle in relation to production system and implications for human nutrition. Meat Sci. 49:329-341. DOI ScienceOn |
13 | Gibson, R. A., M. A. Neumann and M. Makrides. 1997. Effect of increasing breast milk docosahexaenoic acid on plasma and erythrocyte phospholipid fatty acids and neural indices of exclusively breast fed infants. Eur. J. Clin. Nutr. 51:578-584. DOI ScienceOn |
14 | He, C., X. Qu, L. Cui, J. Wang and J. X. Kang. 2009. Improved spatial learning performance of fat-1 mice is associated with enhanced neurogenesis and neuritogenesis by docosahexaenoic acid. Proc. Natl. Acad. Sci. USA. pp. 11370-11375. |
15 | Kang, J. X. 2011. Omega-3: A link between global climate change and human health. Biotechnol. Adv. 29:388-390. DOI ScienceOn |
16 | Kang, J. X. and J. Wang. 2005. A simplified method for analysis of polyunsaturated fatty acids. BMC Biochem. 6:5. DOI ScienceOn |
17 | Sakuradani, E., T. Abe, K. Iguchi and S. Shimizu. 2005. A novel fungal omega3-desaturase with wide substrate specificity from arachidonic acid-producing Mortierella alpina 1S-4. Appl. Microbiol. Biotechnol. 66:648-654. DOI ScienceOn |
18 | Norsker, N. H. and J. G. Støttrup. 1994. The importance of dietary HUFAs for fecundity and HUFA content in the harpacticoid, Tisbe holothuriae Humes. Aquaculture 125:155-166. DOI ScienceOn |
19 | Pan, D., L. Zhang, Y. Zhou, C. Feng, C. Long, X. Liu, E. Dong, S. Wang, R. Wan, J. Zhang and H. Chen. 2009. Production of fat1 transgene pig by somatic cell nuclear transfer. Science in China 39:295-302. |
20 | Pereira, S. L., Y. S. Huang, E. G. Bobik, A. J. Kinney, K. L. Stecca, J. C. Packer and P. Mukerji. 2004, A novel omega3-fatty acid desaturase involved in the biosynthesis of eicosapentaenoic acid. Biochem. J. 378:665-671. DOI ScienceOn |
21 | Spychalla, J. P., A. J. Kinney and J. Browse. 1997. Identification of an animal omega-3 fatty acid desaturase by heterologous expression in Arabidopsis. Proc. Natl. Acad. Sci. USA 94:142-1147. DOI ScienceOn |
22 | Stothard, P. 2000. The sequence manipulation suite: JavaScript programs for analyzing and formatting protein and DNA sequences. Biotechniques 28:1102, 1104. |
23 | Wright, F. 1990. The 'effective number of codons' used in a gene. Gene 87:23-29. DOI ScienceOn |
24 | Zhu, G., H. Chen, X.Wu, Y. Zhou, J. Lu and J. Deng. 2008. A modified n-3 fatty acid desaturase gene from Caenorhabditis briggsae produced high proportion of DHA and DPA in transgenic mice. Transgenic Res. 17:717-725. DOI ScienceOn |
25 | Lai, L., J. X. Kang, R. Li, J. Wang, W. T. Witt, H. Y. Yong, Y. Hao, D. M. Wax, C. N. Murphy and A. Rieke et al. 2006. Generation of cloned transgenic pigs rich in omega-3 fatty acids. Nat. Biotechnol. 24:435-436. DOI ScienceOn |
26 | Lecoeur, H. 2002. Nuclear apoptosis detection by flow cytometry: influence of endogenous endonucleases. Exp. Cell Res. 277:1-14. DOI ScienceOn |
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