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QTL mapping of low-temperature germinability and identification of qLTG1 candidate genes in rice

  • Kim, Sun Ha;Shim, Kyu-Chan;Lee, Hyun-Sook;Le, Anh Quynh;Ahn, Sang-Nag
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.116-116
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    • 2017
  • Low-temperature is one of the environmental stress factors that affect plant growth and development and consequently limit crop productivity. The control of seed germination under low-temperature is organized by many genes which are called quantitative trait loci (QTLs). High germination rate for low-temperature is an important factor of growing rice. Previously, we identified a major QTL controlling low-temperature germinability in rice using 96 introgression lines (ILs) derived from a cross between Oryza rufipogon (Rufi) and the Korean japonica cultivar, 'Hwaseongbyeo (HS)'. A $BC_3F_7$ line (TR5) showed better low-temperature germinability than its recurrent parent. TR5 was crossed with HS to develop a segregating F2:3 populations for the target QTL. Six SSR markers polymorphic between HS and Rufi were used to screen and fine map the qLTG1. The qLTG1 on chromosome 1, which accounted for 55.5% of the total phenotypic variation, confirmed that Rufi allele enhanced the low-temperature germinability. Intervals between markers CRM16 and CRM15, four candidate genes were identified. The identified candidate genes, which are encoded by a protein of unknown function, showed their direct involvement on seed germination at low-temperature. To identify genes targeted by qLTG1, we investigated the expression profiles of these candidate genes and germination behavior of qLTG1 under different stress conditions and compared to HS, Rufi, and TR5 at $13{\pm}2^{\circ}C$ for 3 days after incubation. Furthermore, transgenic rice plants will also be developed to conduct a detailed investigation on low-temperature germinability. Hence, the QTL for low-temperature germinability would be useful in rice breeding programs especially in the development of lines possessing low-temperature germinability.

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PAGAN II: THE EVOLUTION OF AGN JETS ON SUB-PARSEC SCALES

  • OH, JUNGHWAN;TRIPPE, SASCHA;KANG, SINCHEOL;KIM, JAE-YOUNG;PARK, JONG-HO;LEE, TAESEOK;KIM, DAEWON;KINO, MOTOKI;LEE, SANG-SUNG;SOHN, BONG WON
    • Journal of The Korean Astronomical Society
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    • v.48 no.5
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    • pp.299-311
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    • 2015
  • We report first results from KVN and VERA Array (KaVA) VLBI observations obtained in the frame of our Plasma-physics of Active Galactic Nuclei (PAGaN) project. We observed eight selected AGN at 22 and 43 GHz in single polarization (LCP) between March 2014 and April 2015. Each source was observed for 6 to 8 hours per observing run to maximize the uv coverage. We obtained a total of 15 deep high-resolution images permitting the identification of individual circular Gaussian jet components and three spectral index maps of BL Lac, 3C 111 and 3C 345 from simultaneous dual-frequency observations. The spectral index maps show trends in agreement with general expectations – flat core and steep jets – while the actual value of the spectral index for jets shows indications for a dependence on AGN type. We analyzed the kinematics of jet components of BL Lac and 3C 111, detecting superluminal proper motions with maximum apparent speeds of about 5c. This constrains the lower limits of the intrinsic component velocities to ~ 0.98c and the upper limits of the angle between jet and line of sight to ~20°. In agreement with global jet expansion, jet components show systematically larger diameters d at larger core distances r, following the global relation d ≈ 0.2r, albeit within substantial scatter.

A study on the identification of type IIa natural diamonds treated by the HPHT method (HPHT(고온고압)에 의해 처리된 type IIa 천연 다이아몬드의 감별에 관한 연구)

  • 김영출;최현민
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.14 no.1
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    • pp.21-26
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    • 2004
  • Results from PL and Raman spectroscopic analyses of HPHT (high-pressure high-temperature) treated type IIa diamonds are presented, and these spectral characteristics are compared with those of untreated diamonds of similar color and type. We identify a number of significant changes by 325 nm He/Cd laser excitation. Several peaks are removed completely, including H4, H3 system in HPHT treated diamond. The N3 system, however, increased in emission. Also we can find the behaviour of the nitrogen-vacancy related center N-V centers at 575 and 637.1 nm, as observed with 514 nm Ar ion laser excitation. When these centers are present, the FWHM (full width at half maximum) of 637.1 nm luminescence intensities offers a potential means of separating HPHT-treated from untreated type IIa diamonds. The width of 637.1 nm $(N-V)^-$line measured at the position oi half the peak's height are determine to range from 19.8 to $32.1cm^{-1}$ for HPHT treated diamonds.

Low Power UHF Tag Chip Design (저 전력 UHF 태그 칩 설계)

  • Kwon, Hyuk-Je;Lee, Pyeong-Han;Lee, Chul-Hee;Kim, Chong-Kyo
    • Journal of the Institute of Electronics Engineers of Korea SD
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    • v.45 no.12
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    • pp.47-56
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    • 2008
  • An RFID system comprises a reader and a tag, and this paper focuses on a tag design. A UHF tag is activated by energy supply using electromagnetic waves and energy reflection through impedance mismatching. The tag uses a $0.25{\mu}m$ CMOS process and comprises a digital part executing tag protocols, a 512-bit memory, and an analog part having a rectifier, a modulation/demodulation unit, a clock generator, etc. The total dimension of the tag, including a saw line, is $750{\mu}m*750{\mu}m$ and the power consumption of the tag consumption power is about $17.8{\mu}W$ at a supply voltage of 2V.

A Attendance-Absence Checking System using the Self-organizing Face Recognition (자기조직형 얼굴 인식에 의한 학생 출결 관리 시스템)

  • Lee, Woo-Beom
    • The Journal of the Korea Contents Association
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    • v.10 no.3
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    • pp.72-79
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    • 2010
  • A EAARS(Electronic Attendance-Absence Recording System) is the important LSS(Learning Support System) for blending a on-line learning in the face-to-face classroom. However, the EAARS based on the smart card can not identify a real owner of the checked card. Therefore, we develop the CS(Client-Sever) system that manages the attendance-absence checking automatically, which is used the self-organizing neural network for the face recognition. A client system creates the ID file by extracting the face feature, a server system analyzes the ID file sent from client system, and performs a student identification by using the Recognized weight file saved in Database. As a result, The proposed CS EAARS shows the 92% efficiency in the CS environment that includes the various face image database of the real classroom.

Anti-proliferative and angio-suppressive effect of Stoechospermum marginatum (C. Agardh) Kutzing extract using various experimental models

  • Vinayak, Rashmi;Puttananjaiah, Shilpa;Chatterji, Anil;Salimath, Bharati
    • Nutrition Research and Practice
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    • v.8 no.4
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    • pp.377-385
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    • 2014
  • BACKGROUND/OBJECTIVES: Abundant consumption of seaweeds in the diet is epidemiologically linked to the reduction in risk of developing cancer. In larger cases, however, identification of particular seaweeds that are accountable for these effects is still lacking, hindering the recognition of competent dietary-based chemo preventive approaches. The aim of this research was to establish the antiproliferative potency and angiosuppressive mode of action of Stoechospermum marginatum seaweed methanolic extract using various experimental models. MATERIALS/METHODS: Among the 15 seaweeds screened for antiproliferative activity against Ehrlich ascites tumor (EAT) cell line, Stoechospermum marginatum extract (SME) was found to be the most promising. Therefore, it was further investigated for its anti-proliferative activity in-vitro against choriocarcinoma (BeWo) and non-transformed Human embryonic kidney (HEK 293) cells, and for its anti-migratory/tube formation activity against HUVEC cells in-vitro. Subsequently, the angiosuppressive activity of S. marginatum was established by inhibition of angiogenesis in in-vivo (peritoneal angiogenesis and chorioallantoic membrane assay) and ex-vivo (rat cornea assay) models. RESULTS: Most brown seaweed extracts inhibited the proliferation of EAT cells, while green and red seaweed extracts were much less effective. According to the results, SME selectively inhibited proliferation of BeWo cells in-vitro in a dose-dependent manner, but had a lesser effect on HEK 293 cells. SME also suppressed the migration and tube formation of HUVEC cells in-vitro. In addition, SME was able to suppress VEGF-induced angiogenesis in the chorio allantoic membrane, rat cornea, and tumor induced angiogenesis in the peritoneum of EAT bearing mice. A decrease in the microvessel density count and CD31 antigen staining of treated mice peritoneum provided further evidence of its angiosuppressive activity. CONCLUSIONS: Altogether, the data underline that VEGF mediated angiogenesis is the target for the angiosuppressive action of SME and could potentially be useful in cancer prevention or treatment involving stimulated angiogenesis.

Two-Phase Localization Algorithm in Wireless Sensor Networks (무선 센서 네트워크에서의 2단계 위치 추정 알고리즘)

  • Song Ha-Ju;Kim Sook-Yeon;Kwon Oh-Heum
    • Journal of Korea Multimedia Society
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    • v.9 no.2
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    • pp.172-188
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    • 2006
  • Sensor localization is one of the fundamental problems in wireless sensor networks. Previous localization algorithms can be classified into two categories, the GGB (Global Geometry-Based) approaches and the LGB (Local Geometry-Based). In the GGB approaches, there are a fixed set of reference nodes of which the coordinates are pre-determined. Other nodes determine their positions based on the distances from the fixed reference nodes. In the LGB approaches, meanwhile, the reference node set is not fixed, but grows up dynamically. Most GGB algorithms assume that the nodes are deployed in a convex shape area. They fail if either nodes are in a concave shape area or there are obstacles that block the communications between nodes. Meanwhile, the LGB approach is vulnerable to the errors in the distance estimations. In this paper, we propose new localization algorithms to cope with those two limits. The key technique employed in our algorithms is to determine, in a fully distributed fashion, if a node is in the line-of-sight from another. Based on the technique, we present two localization algorithms, one for anchor-based, another for anchor-free localization, and compare them with the previous algorithms.

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Design and Implementation of Index for RFID Tag Objects (RFID 태그 객체를 위한 구간 색인 구조의 설계 및 구현)

  • Ban, Chae-Hoon;Hong, Bong-Hee
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2008.10a
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    • pp.143-146
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    • 2008
  • For tracing tag locations, a trajectories should be modeled and indexed in radio frequency identification (RFID) systems. The trajectory of a tag can be represented as a line that connects two spatiotemporal locations captured when the tag enters and leaves the vicinity of a reader. If a tag enters but does not leave a reader, its trajectory is represented only as a point captured at entry and we should extend the region of a query to find the tag that remains in a reader. In this paper, we propose an interval data model of tag's trajectory in order to solve the problem. For the interval data model. we propose a new index scheme called the IR-tree(Interval R-tree) and algorithms of insert and split for processing query efficiently. We also evaluate the performance of the proposed index scheme and compare it with the previous indexes.

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An Interval Data Model for Tracing RFID Tag Objects (RFID 태그 객체의 위치 추적을 위한 구간 데이터 모델)

  • Ban, Chae-Hoon;Hong, Bong-Hee
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2007.10a
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    • pp.578-581
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    • 2007
  • For tracing tag locations, a trajectories should be modeled and indexed in radio frequency identification (RFID) systems. The trajectory of a tag can be represented as a line that connects two spatiotemporal locations captured when the tag enters and leaves the vicinity of a reader. If a tag enters but does not leave a reader, its trajectory is represented only as a point captured at entry. Because the information that the tag stays in the reader is missing from the trajectory represented only as a point, we should extend the region of a query to find the tag that remains in a reader. In this paper, we propose an interval data model of tag's trajectory in order to solve the problem. Trajectories of tags are represented as two kinds of intervals; dynamic intervals which are time-dependent lines and static intervals which are fixed lines. We also show that the interval data model has better performance than others with a cost model

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A Knowledge-based System for Analyzing Sophisticated Geometric Structure of Document Images (문서 영상의 정교한 기하적 구조분석을 위한 지식베이스 시스템)

  • Lee, Kyong-Ho;Choy, Yoon-Chul;Cho, Sung-Bae
    • Journal of KIISE:Software and Applications
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    • v.28 no.11
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    • pp.795-813
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    • 2001
  • Sophisticated geometric structure analysis must be preceded to create electronic document from logical components extracted from document image. this paper presents a knowledge-based method for sophisticated geometric structure analysis of technical journal pages. The proposed knowledge base encodes geometric characteristics that are not only common in technical journals but also publication-specific in the form rules. The method takes the hybrid of top-down and bottom-up techniques and consists of two phases: region segmentation and identification. Generally, the result of segmentation process does not have a one-to-one matching with composite layout components. Therefore, the proposed method identifies non-text objects such as image, drawing and table, as well as text objects such as text line and equation by splitting or grouping segmented regions into composite layout components. Experimental results with 372 images scanned from the IEEE Transactions on Pattern Analysis and Machine Intelligence show that the proposed method has performed geometrical structure analysis successfully on more than 99% of the test images, resulting in sophisticated performance compared with previous works.

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