• Plant Resources
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• 제4권3호
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• pp.181-188
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• 2001

We tried to introduce two forsythia genes related in lignan biosynthesis, dirigent protein and pinoresinol/lariciresinol (Ph) reductase, into potatoes for accumulation of lignans in transgenic potatoes. We made binary vectors overexpressing dirigent protein gene and P/L reductase gene driven by a CaMV35S promoter and transformed into potatoes via Agrobacterium mediated transformation. And in order to control the metabolic flux of lignan biosynthesis pathway, we tried to inhibit chalcone synthase genes of potatoes by antisense inhibition technique also. We tried to use PCR screening method for selection of transgenic plants of different vectors. We tried to determine and compare lignan contents from different transgenic potato lines.

• Archives of Pharmacal Research
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• 제28권9호
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• pp.1023-1026
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• 2005

A new furofuran lignan (1) was isolated from the n-butanol fraction of the methanolic extract of the leaves of Petasites japonicus (Sieb. et Zucc.) Maxim. (Compositae). The structure of compound 1 was determined to be $2{\alpha}-(4'-hydroxy-3'-methoxyphenyl)-6{\alpha}-(4"-hydroxy-3"-methox­yphenyl)-8{\alpha}-hydroxy-3, 7-dioxabicyclo[3.3.0]octane\;4'-O-({\beta}-D-glucopyranoside)$ by spectroscopic methods including 2D-NMR. In further studies, it was found that the compound 1 expressed an antioxidant activity in DPPH radical scavenging assay, and moreover, ameliorated the seizure in kainic acid-treated mice.

• 한국연초학회지
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• 제11권2호
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• pp.225-232
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• 1989

Deoxyschizandrin, gomisin N, schizandrin, wuweizisu C, gomisin A and angeloylgomisin H were isolated from fruits of Schisandra chinensis BAILLON, and the lignan contents in shred and smoke of 'Balloon-Flower' cigarettes were quantitatively analyzed by capillary-GC(FID). The GC column was SPB-1 fused silica capillay (0.25mm id$\times$30m, Supelco) and the column oven temperature was programmed from 20$0^{\circ}C$ to 30$0^{\circ}C$ at the rate of 4$^{\circ}C$/min. In the shred of 'Balloon-Flower' cigarettes deoxyschizandrin. gomisin N, schizandrin, gomisin A and angeloylgomisin H were detected and schizandrein contets were the highest among them, 22.77$\mu\textrm{g}$/cig., gomisin N, schizandrin and gomisin A were 0.023, 0.054, 0.0849 and 0.167$\mu\textrm{g}$/cig. respectively, and angeloylgomisin H was not detected.

• Archives of Pharmacal Research
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• 제24권3호
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• pp.198-201
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• 2001

Four lignan xylosides and two neolignan glycosides were isolated from the stem and root barks of Ulmus davidiana var. japonica. Their structures were identified as lyoniside, nudiposide, 5'-methoxyisolarciresinol-9'-O-$\beta$- D-xylopyranoside, isolariciresiol-9'-O-$\beta$-D-xylopyranoside, rel-trans-dihydrodehydroconiferyl alcohol 4'-O-$\alpha$-L-rhamnopyranoside and icariside E3 by comparison of their spectral data with those reported in the literatures, respectively.

• 한국작물학회지
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• 제51권1호
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• pp.73-80
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• 2006

Sesame (Sesamum indicum L.) seeds contain abundant oil and antioxidative lignans related to the seed quality. To evaluate the potential effects of drought stress on the chemical composition of sesame seeds, eighteen cultivars were imposed water-deficit condition by withholding irrigation during 15 days at podding and maturing stage, compared with well-watered plants as control in seed yield and chemical composition. Drought treatments showed great decrease of seed yield with not affecting seed weight. The contents of sesamin and sesamolin decreased while lignan glycosides inversely increased in response to drought stress. Oil content was not significantly changed by drought treatment in spite of its slight decrease. In case of fatty acid composition, there were significant differences in increase of oleic acid while inverse decrease of linoleic acid under drought stress condition. These results demonstrate that the chemical composition of sesame seed may be modified with drought stress. In particular, the increase of sesaminol glucosides with strong antioxidative activity was observed.

• Natural Product Sciences
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• 제28권2호
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• pp.53-57
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• 2022

As part of our continued study on the chemical constituents of Willughbeia edulis stems, a new dimeric lignan named edulignan (1) was isolated from its EtOAc-soluble extract. Based on NMR spectroscopic interpretation, the planar structure of 1 has been suggested to have two 2-substituted 4-chromanone subunits with different stereochemical configurations. In addition, the MS/MS analysis of the products obtained by acid-catalyzed hydrolysis of 1 was supportive of its structure. Unfornatually, the new compound 1 did not show 𝛼-glucosidase inhibitory activity with an IC₅₀ value > 250 𝜇M.

• 한국작물학회지
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• 제67권1호
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• pp.61-66
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• 2022

본 연구는 참깨에 함유된 세사민 및 세사몰린의 함량을 비파괴적으로 신속하게 평가하기 위하여 NIRS 분석을 이용해 검량식을 작성하고 검량식의 적용가능성을 검증하였다. 검량식 작성에 사용된 482점 참깨의 HPLC 분석 결과를 NIRS 스펙트럼에 적용시킨 후 검량식을 작성하였다. 세사민 및 세사몰린의 R² 값은 각각 0.936, 0.875로 조사되었으며 이를 cross validation 한 결과에서도 각각 0.899, 0.781로 조사되어 리그난 함량 분석에 적용 가능할 것으로 판단되었다. 작성된 검량식의 적용가능성을 확인하기 위해 2020년에 생산된 참깨 유전자원 90종의 종자를 NIRS를 통해 분석한 결과 세사민 및 세사몰린의 R²값이 각각 0.653, 0.596으로 크게 낮아졌으나 리그난 함량이 높은 상위 30%의 자원을 선발하는데 무리가 없었다. 따라서 본 연구에서 작성된 NIRS 검량식은 육종 초기에 고리그난 함량을 선발하는데 적용 가능할 것으로 판단된다.

• Journal of Applied Biological Chemistry
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• 제66권
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• pp.467-476
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• 2023

오미자(Schisandra chinensis)는 오미자과에 속하는 낙엽활엽덩굴식물로 한국, 일본, 중국, 대만 등 동아시아에 널리 분포한다. 오미자에 함유된 주요 성분에는 리그난 화합물뿐만 아니라 트리테르페노이드 화합물도 포함되어 있는 것으로 보고되었다. 한국 산지별 오미자의 특성을 구별하기 위해 대사산물 프로파일링과 다변량 통계 분석 기법인 PCA을 수행하여 판별식을 설정하였고, 그 결과 triterpenoids 16종, lignan 9종, flavonoid, phenylpropanoid, fatty acid 각 1종을 동정하였다. 또한 다변량 통계분석을 통해 OPLS-DA의 s-plot 모델을 적용하여 단양, 문경, 거창, 평창의 4개 그룹을 구분하는 것을 확인하였고, lanostane, cycloartane, 그리고 schiartane triterpenoid, dibenzocyclooctadiene lignan 이 각각 화학적마커로 동정하였다.

• 약학회지
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• 제48권4호
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• pp.236-240
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• 2004

Acanthopanax species (Araliaceae) traditionally has been used as analgesics, stimulant of immune system, and replenishment of body functions. Acanthopanax divaricatus var. albeofructus is indigenous plant to Korea. The antioxidant activities of compounds from A divaricatus var. albeofructus were determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) method and thiobarbituric acid reactive substance (TBARS) assay on human plasma low-density lipoprotein (LDL). The triterpenoid and lignan constituents from this plant showed antioxidant activities and the lignan, l-sesamin exhibited the most potent antioxidant activity in Cu$^{2+}$ -induced LDL oxidation.n.

• 생약학회지
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• 제48권4호
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• pp.285-288
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• 2017

A lignan and four flavonoid derivatives were isolated from the aerial parts of Panicum dichotomiflorum Michaux (Gramineae) through repeated column chromatography. Their chemical structures were identified as (-)-pinoresinol (1), tricin (2), luteolin (3), luteolin-4'-O-${\beta}$-D-glucopyranosde (4) and luteolin-7-O-${\beta}$-D-glucopyranosde (5), respectively, by spectroscopic analysis. These compounds were isolated for the first time from this plant.