• Title/Summary/Keyword: Light exposure time

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Effect of Rhus verniciflua Stokes Extract and Fisetin on UVB-induced Apoptosis in NIH3T3 Cells (NIH3T3 세포에서 UVB에 의한 세포고사에 미치는 옻 추출물과 fisetin의 효과)

  • Kim Don Young;Hwang Eun Hee;Park Jong Kun
    • Journal of Life Science
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    • v.15 no.1 s.68
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    • pp.141-146
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    • 2005
  • For a long time Rhus vemiciflua Stokes (RVS) has been traditionally used as a herbal plant in Asia. In this study, we have investigated the effect of acetone extract of Rhus verniciflua Stokes (RVSE) and fisetin, a component of RVSE, on DNA damage response in NIH3T3 cells. Exposure of cells to DVB light $(200 J/m^2)$ and postincubation in growth medium for 48 hr resulted in a decrease of cell viability to about $10-20\%$ of nontreated control. Addition of various concentrations of RVSE in the postincubation medium, however, significantly increased the cell viability as compared with the values expected. The genotoxicity-decreasing effect was also demonstrated in cells exposed to UVB light and incubated in medium containing fisetin. The genotoxicity-decreasing effect of RVSE and fisetin was further demonstrated by various analyses including cell morphology studies, trypan blue exclusion assay and DAPI staining. By Annexin V binding analysis, RVSE and fisetin were shown to decrease the early apoptosis induced by UVB exposure. These results suggest the RVSE contain components that either increase the DNA repair or decrease the apoptosis in UVB-exposed cells.

COMPARISON OF THE DECREE OF CONVERSION IN LIGHT-CURED COMPOSITE RESIN CURED BY HALOGEN AND PLASMA XENON ARC LAMP CURING UNIT (Halogen lamp 광조사기와 Plasma xenon arc lamp 광조사기에 의한 광중합 복합레진의 중합률 비교)

  • Lee, Young-Jun;Jeong, Byung-Cho;Choi, Nam-Ki;Yang, Kyu-Ho
    • Journal of the korean academy of Pediatric Dentistry
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    • v.29 no.3
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    • pp.328-336
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    • 2002
  • Recently, new light curing unit utilizing the plasma xenon arc lamp is introduced. This curing unit is operated at relatively high intensity, so shortening the curing time significantly. The aim of this experiment was to estimate curing capability of plasma xenon arc lamp curing unit compared to traditional halogen lamp curing unit. Degree of conversion was evaluated by Raman spectroscopy after irradiation of specimens with halogen lamp curing unit(Optilux 150, Demetron, USA) for 20s, 40s, 60s and plasma xenon arc lamp curing unit(flipo, Lokki, France) for 2s, 3s, 6s. The results showed that strong light intensity of plasma xenon arc lamp curing unit did not compensate for short exposure time completely. So, Multi-layered curing within 2mm thickness and additional exposure time is recommanded when light-cured composite resin is polymerized with plasma xenon arc lamp curing unit.

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Involvement of Nitric Oxide and Prostanoid on Photorelaxation in Pig Renal Artery (UV-light 에 의한 혈관 이완작용에 있어서 nitric oxide와 prostanoid의 관련성)

  • Kim, Joo-Heon;Shim, Cheol-Soo;Jeon, Seok-Cheol
    • Korean Journal of Veterinary Research
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    • v.42 no.3
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    • pp.321-326
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    • 2002
  • The effect of nitric oxide synthase(NOS) inhibita, $N^G$-nitro-L-arginine-methyl ester(L-NAME) and prostanoid synthesis inhibiter, indomethacin on the photorelaxation, when was exposed to the long-wave length UV-light, was examined on the precontraction by the phenylephrine in the isolated pig renal artery. 1. UV-light relaxed both with-endothelium and without-endothelium in the pig renal arterial ring contracted by the phenylephrine. The magnitude of photorelaxation was dependent on the exposure time for UV-light. 2. UV-Iight induced relaxation was inhibited by L-NAME and indomethacin on the precontraction by the phenylephrine in the isolated pig renal artery. 3. UV-Iight induced relaxation was inhibited by methylene blue on the precontraction by the phenylephrine in the isolated pig renal artery. These results suggest that UV-light induced photorelaxation may be due to cGMP involved both nitric oxide and prostanoid on the precontraction by the phenylephrine in the isolated pig renal artery.

Assessment of Exposure to and Risk of Formaldehyde and Particulate Matter (PM10 and PM2.5) by Time Activity Applying Real-Time Indoor and Outdoor Monitoring (실내·외 실시간 모니터링을 활용한 폼알데하이드 및 미세먼지(PM10, PM2.5)의 거주시간별 노출 및 위해도 평가)

  • Yoon, Danki;Namgoung, Sunju;Kong, Hyekwan;Hong, Hyungjin;Lim, Huibeen;Park, Sihyun;Lee, Hyewon;Lee, Jungsub;Lee, Cheolmin
    • Journal of Environmental Health Sciences
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    • v.45 no.6
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    • pp.646-657
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    • 2019
  • Objectives: The purpose of this study was to suggest methods to investigate continuous monitoring of concentration levels and assess the exposure of individuals considering the actual time activity of residents for formaldehyde and particulate matter (PM10, PM2.5) in the indoor and outdoor air of a house, assess the health risks of children and adults based on the results of the exposure assessment, and provide basic data on studies for assessing exposure and health risks in Korea in the future. Methods: The concentration levels of formaldehyde and particulate matter were measured in a family home in Gyeonggi-do Province from April 25 to July 31, 2019, using electrochemical sensors (formaldehyde) and light scattering sensors (PM10, PM2.5). Risk assessment by the duration of exposure by time activity was performed by dividing between weekdays and weekends, and indoors and outdoors. Results: The greatest level of carcinogenic risk from inhaling formaldehyde was indoors during the weekdays for both children and adults. For children, the risk was at 7.5 per approximately 10,000 people, and for adults, the risk was at 4.1 per approximately 10,000 people. PM10 and PM2.5 also showed the greatest values indoors during the weekdays, with children at 1.7 people and 1.4 per approximately 100 people, respectively, and adults at 8.2 per approximately 1,000 and 1.8 per approximately 100 people, respectively. Conclusions: The risks of formaldehyde, PM10 and PM2.5 were shown to be high indoors. Therefore, consideration of exposure assesment for each indoor pollutant and management of indoor air quality is necessary.

The Influence of Experiment Variables on DLP 3D Printing using ART Resin (ART 수지의 DLP 3D Printing 가공 시 실험변수의 영향)

  • Shin, Geun-Sik;Kweon, Hyun-Kyu;Kang, Yong-Goo
    • Journal of the Korean Society of Manufacturing Process Engineers
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    • v.16 no.6
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    • pp.101-108
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    • 2017
  • Recently, the patent rights for 3D printing technology have expired, while 3D printers with RP (Rapid Prototyping or Additive Manufacturing) and 3D printing technologies are receiving attention. In particular, the development of 3D printers is rapid in Korea, thanks to the increasing sales and popularity of FDM (Fused Deposition Modeling or Fused Filament Fabrication) 3D printers. However, the quality and productivity of the FDM 3D Printer are not good, so customers prefer the DLP (Digital Light Processing) method to avoid these shortcomings. The DLP method has high quality and productivity. However, because of the stereolithography equipment, it has few studies compared to optimal values for elements then FDM 3D printing study. In this study, to find the optimal conditions for 3D printing with the DLP method, the aim is to obtain the optimal values (strength, final time, quality) by changing the light exposure time, layer thickness, and z-axis speed.

Development of an AutoFlat program for the acquisition of effective flat images in the automated observation system

  • Yoon, Joh-Na;Kim, Yonggi;Kim, Dong-Heun;Yim, Hong-Suh
    • Journal of Astronomy and Space Sciences
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    • v.30 no.4
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    • pp.327-334
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    • 2013
  • The purpose of this study is to develop an observation program for obtaining effective flat images that are necessary for photometric observation. The development of the program was achieved by improving the existing method for obtaining twilight flat images. The existing method for obtaining twilight flat images acquires flat images by observing the sky light after sunset or light before sunrise. The decision of when to observe flat images at each night is solely dependent on the judgment of an observer, and thus the obtained flat images for particular nights may not be clean. Especially, in the case of the observatories where an automated observation system is in operation, there is a difficulty that an observer should pay attention during sunrise and sunset in order to obtain flat images. In this study, a computer program is developed to improve this inconvenience and to efficiently perform photometric observation in the observatories where an automated observation system is applied. This program can obtain flat images by calculating the time for obtaining flat images automatically and the exposure time using a numerically calculated function. When obtaining twilight flat images at dusk and at dawn, the developed program performs automated observation and provides effective flat images by acquiring appropriate exposure time considering the sunrise and sunset times that vary depending on the day of observation. The code for performing this task was added to Obs Tool II (Yoon et al. 2006), which is the automated observation system of the Chungbuk National University Observatory, and the usefulness of the developed program was examined by performing an actual automated observation. If this program is applied to other observatories where automated observation is in operation, it is expected that stable and high-quality flat images could be obtained, which can be used for the pre-processing of photometric observation data.

Degree of conversion of two dual-cured resin cements light-irradiated through zirconia ceramic disks

  • Kim, Min-Jeong;Kim, Kyo-Han;Kim, Young-Kyung;Kwon, Tae-Yub
    • The Journal of Advanced Prosthodontics
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    • v.5 no.4
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    • pp.464-470
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    • 2013
  • PURPOSE. The aim of this Fourier transform infrared (FTIR) spectroscopic study was to measure the degree of conversion (DC) of dual-cured resin cements light-irradiated through zirconia ceramic disks with different thicknesses using various light-curing methods. MATERIALS AND METHODS. Zirconia ceramic disks (KT12) with three different thicknesses (1.0, 2.0, and 4.0 mm) were prepared. The light transmittance of the disks was measured using ultraviolet visible near-infrared spectroscopy. Four different light-curing protocols were used by combining two curing light modes (Elipar TriLight (standard mode) and bluephase G2 (high power mode)) with light-exposure times of 40 and 120 seconds. The DCs of the two dual-cured resin cements (Duo-Link and Panavia F2.0) light-irradiated through the disks was analyzed at three time intervals (3, 7, and 10 minutes) by FTIR spectroscopy. The data was analyzed using repeated measures ANOVA (${\alpha}$=.05).Two-way ANOVA and Tukey post hoc test were used to analyze the 10 minute DC results. RESULTS. The 1.0 mm thick disk exhibited low light transmittance (<25%), and the transmittance decreased considerably with increasing disk thickness. All groups exhibited significantly higher 10 minute DC values than the 3 or 7 minute values (P<.05), but some exceptions were observed in Duo-Link. Two-way ANOVA revealed that the influence of the zirconia disk thickness on the 10 minute DC was dependent on the light-curing methods (P<.001). This finding was still valid even at 4.0 mm thickness, where substantial light attenuation took place. CONCLUSION. The curing of the dual-cured resin cements was affected significantly by the light-curing technique, even though the additional chemical polymerization mechanism worked effectively.

Effects of Light Pollution from Mobile Digital Devices on Sleep and Circadian Rhythms (모바일 디지털 기기로 인한 빛공해가 수면과 일주기 리듬에 미치는 영향)

  • Lee, Youn-Jung;Cho, Chul-Hyun;Lee, Heon-Jeong
    • Sleep Medicine and Psychophysiology
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    • v.27 no.1
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    • pp.1-7
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    • 2020
  • Mobile digital devices are very familiar and useful devices in the daily life of modern people, and are used for various tasks such as communication, reading, writing, and playing media. As the use of mobile digital devices has become more prevalent, user time has also been increasing. In particular, the number of people who use digital devices before sleep is growing. The light pollution associated with these devices is classified into four categories: urban sky glow, glare, light trespass, and clutter. The pattern in which modern people use digital devices corresponds to light pollution caused by light trespass and clutter from light exposure to artificial light at night. The light pollution caused by digital devices can cause melatonin secretion suppression, delayed sleep onset, reduction of sleepiness before bedtime, and periodic rhythm and cognitive function disturbances. In addition, a study of children and adolescents showed there may be disturbances in the sleep-wake cycle and circadian rhythm, deterioration of sleep quality, and daytime fatigue due to light pollution caused by artificial light at night from mobile digital devices. A multi-faceted research effort is also necessary to investigate the healthy use of mobile digital devices based on research evidence and insights with an accurate evaluation of the influence of mobile digital devices as a form of light pollution.

DEEP-South: Asteroid Light-Curve Survey Using KMTNet

  • Lee, Hee-Jae;Yang, Hongu;Kim, Dong-Heun;Kim, Myung-Jin;Moon, Hong-Kyu;Kim, Chun-Hwey;Choi, Young-Jun
    • The Bulletin of The Korean Astronomical Society
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    • v.45 no.1
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    • pp.46.3-47
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    • 2020
  • Variations in the brightness of asteroids are caused by their spins, irregular shapes and companions. Thus, in principle, the spin state and shape model of a single object or, a combined model of spins, shapes and mutual orbit of a multiple components can be constructed from the analysis of light curves obtained from the time-series photometry. Using ground- and space-based facilities, a number of time-series photometric observations of asteroids have been conducted to find the possible causes of their light variations. Nonetheless, only about 2% of the known asteroids have been confirmed for their rotation periods. Therefore, a follow-on systematic photometric survey of asteroids is essential. We started an asteroid light curve survey for this purpose using Korea Microlensing Telescope Network (KMTNet) during 199 nights between the second half of 2019 and the first half of 2020. We monitored within a 2° × 14° region of the sky per each night with 25 min cadences. In order to observe as many asteroids as possible with a single exposure, we mostly focus on the ecliptic plane. In our survey, 25,925 asteroids were observed and about 8,000 of them were confirmed for their rotation periods. In addition, using KMTNet's 24-hour continuous monitoring, we collected many composite light curves of slow rotating asteroids that were rarely obtained with previous observations. In this presentation, we will introduce the typical light curves of asteroids obtained from our survey and present a statistical analysis of spin states and shapes of the asteroids from this study.

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Inactivation influences on Escherichia coli DS5α by irradiation with 405 nm violet-light

  • Young-Sun Kim;Mun-Jin Choi;Dae-Young Lee;Sang-Ook Kang;Geung-Joo Lee
    • Korean Journal of Agricultural Science
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    • v.50 no.3
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    • pp.459-467
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    • 2023
  • Because an irradiation of 405 nm violet light could have a strong energy, it was used to be sterilized against various microbes in the indoor air condition or fresh food. Escherichia coli is a representative bio-pollutant in the indoor air-borne bacteria, and a hygienic microbe in the horticultural food. This study evaluated the inactivation influences on E. coli DS5α after exposure to 405 nm violet-light (VL) by investigating irradiating time, and the vertical and horizonal distance from light source. The illumination of 405 nm VL was inversely proportional to the distance from the VL source. E. coli DS5α on nutrient agar (NA) was inactivated approximately 50% more than the control when irradiated at 65 cm from 405 nm VL for 3 hours. When compared to the control, E. coli DS5α was inactivated approximately 50% within 70 cm from 405 nm VL for 3 hours. As it was irradiated for 3 hours 70 cm away from 405 nm VL, the horizonal distance from the point was negatively correlated to the inactivation of E. coli DS5α. These results indicated that the inactivation of E. coli DS5α grown on NA medium needs to be irradiated with 405 nm within 70 cm from the light source for 3 hours.