• Journal of Families and Better Life
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• v.15 no.4
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• pp.183-200
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• 1997

The purpose of this study was to identify the stress of remarried elderly in their remarried life. There were 10 remarried elderly respondents. The data was collected by in-depth personal interview. Focusing on the situation of remarried family life the remarried elderly perceived various stress. Those were the stress from the status of remarried person and the stress from relations with their own children/ new spouse/ new spouse's children/ ex-spouse and ex-kin/ sibling and relatives and friends. The results of this study could be a useful material for elderly's remarriage preparation program.

• Korean Journal of Environmental Biology
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• v.41 no.1
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• pp.14-17
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• 2023

A male Spheropistha melanosoma Yaginuma, 1957 from Korea in the family Theridiidae Sundevall, 1833 is described with measurements and morphological photos of the diagnostic characteristics. This species was collected with a sweep net around arable lands in a mountainous mixed forest during the investigation of spider fauna on Ulleungdo Island in 2019.

• Korean Journal of Environmental Agriculture
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• v.39 no.3
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• pp.197-203
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• 2020

BACKGROUND: Most lakes have increased concerns about water pollution due to the inflow of non-point sources caused by human activities. Therefore, the lake water quality survey was conducted in order to propose effective plans for water quality management by analyzing the characteristics of lakes and the change of water quality. METHODS AND RESULTS: In order to investigate the physicochemical water quality in Daedong lake, water quality analysis was undertaken from July 2018 to June 2019. Water temperature was ranged from 7.8 to 34.3℃ and pH varied from 6.9 to 10.2. The concentration of Dissolved oxygen, Suspended solid, Biochemical Oxygen Demand (BOD), and Chemical Oxygen Demand (COD) were 5.6 ~ 17.2 mg/L, 2.4 ~ 35.3 mg/L, and 4.5 ~ 15.1 mg/L, and 0.9 ~ 2.8 mg/L, respectively. The Total Nitrogen (T-N) concentration ranged from 0.974 ~ 2.126 mg/L, and Total Phosphorus (T-P) concentration ranged from 0.014 ~ 0.057 mg/L. The Chlorophyll-a (Chl-a) ranged from 2.7 ~ 37.9 mg/㎥. Through Carlson TSIm assessment using T-P and Chl-a results, evaluating trophic state, Daedong lake was evaluated as mesotrophic. CONCLUSION: Water pollution management plan needs such as nutrient removal technology and nonpoint source management for prevention of eutrophication in Daedong lake.

• Journal of Microbiology and Biotechnology
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• v.30 no.11
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• pp.1680-1687
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• 2020

Fungal endophytes are symbiotic microorganisms that are often found in asymptomatic plants. This study describes the genetic diversity of the fungal endophytes isolated from the roots of plants sampled from the west coast of Korea. Five halophytic plant species, Limonium tetragonum, Suaeda australis, Suaeda maritima, Suaeda glauca Bunge, and Phragmites australis, were collected from a salt marsh in Gochang and used to isolate and identify culturable, root-associated endophytic fungi. The fungal internal transcribed spacer (ITS) region ITS1-5.8S-ITS2 was used as the DNA barcode for the classification of these specimens. In total, 156 isolates of the fungal strains were identified and categorized into 23 genera and two phyla (Ascomycota and Basidiomycota), with Dothideomycetes and Sordariomycetes as the predominant classes. The genus Alternaria accounted for the largest number of strains, followed by Cladosporium and Fusarium. The highest diversity index was obtained from the endophytic fungal group associated with the plant P. australis. Waito-C rice seedlings were treated with the fungal culture filtrates to analyze their plant growth-promoting capacity. A bioassay of the Sm-3-7-5 fungal strain isolated from S. maritima confirmed that it had the highest plant growth-promoting capacity. Molecular identification of the Sm-3-7-5 strain revealed that it belongs to Alternaria alternata and is a producer of gibberellins. These findings provided a fundamental basis for understanding the symbiotic interactions between plants and fungi.

• Microbiology and Biotechnology Letters
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• v.48 no.4
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• pp.439-446
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• 2020

Two Bacillus strains, K3 and K208, both demonstrating strong fibrinolytic activities were isolated from Kimchi, a traditional Korean preparation of fermented vegetables. Isolates were subjected to various molecular biology based identification methods including RAPD-PCR and identified as B. subtilis and B. velezensis, respectively. Tryptic soy broth (TSB) was found to best maintain both the growth and the fibrinolytic activity of these strains. Culture supernatants were analyzed by SDS-PAGE and fibrin zymography, and the results indicate that a 40 and 27 kDa band seem to be responsible for the fibrinolytic activities of these two isolates and the 27 kDa band was subsequently identified as the mature form of AprE, the major fibrinolytic enzyme. Thus the aprE genes were cloned and the translated amino acid sequences demonstrated 99.3% identity with each other, and 86.5% identity with BsfA, a fibrinolytic enzyme from B. subtilis ZA400 also isolated from Kimchi, and AprE2, a fibrinolytic enzyme from B. subtilis CH3-5 isolated from Cheonggukjang, a traditional Korean fermented soy. Given this B. subtilis K3 and B. velezensis K208 may be promising starter cultures in the production of fermented foods.

• Journal of Microbiology and Biotechnology
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• v.18 no.10
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• pp.1630-1633
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• 2008

A novel putative toxin-antitoxin segregational stability system named KyAB system was identified in a novel native plasmid pBMB8240 from Bacillus thuringiensis strain YBT-1520, based on sequences homology with other toxin-antitoxin systems, the lethal activity of the KyB putative toxin in Escherichia coli and the stabilizing effect of the kyAB system in Bacillus thuringiensis. Secondarily, the native plasmid pBMB9741 from the same strain was resequenced and the corrected plasmid was named as pBMB7635. Based on sequence homology with the tasAB system and the lethal activity of toxin protein in Escherichia coli, a tasAB-like putative toxin-antitoxin system was identified on pBMB7635.

• Journal of Korean Academy of Fundamentals of Nursing
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• v.23 no.2
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• pp.172-183
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• 2016

Purpose: The purpose of this study was to explore the subjective experience of life-sustaining treatment care among nurses in intensive care units. Method: A phenomenology was used for the study. Data were collected from October to December, 2015 using open-ended questions during in-depth interviews. Participants were nurses working in intensive care units and were contacted through purposive techniques. Eight nurses participated in this study. Results: Four categories emerged from the analysis using Colaizzi's method: (a) difficulties due to life-sustaining treatment care, (b) dilemma of extension or cessation of life-sustaining treatment, (c) repressed feelings and emotional exhaustion, and (d) forming values for life-sustaining treatment from nursing experience. Conclusion: Provision of clearer guidelines on life-sustaining treatment which reflect a family-oriented culture is important for nurses in ICU and will promote nurses involvement in the decision-making process of life-sustaining treatment of patients.

• Journal of Microbiology and Biotechnology
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• v.25 no.10
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• pp.1660-1669
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• 2015

The present study describes the gene cloning, overexpression and characterization of a novel nitrilase from hyperthermophilic bacterium Thermotoga maritima MSB8. The nitrilase gene consisted of 804 base pairs, encoding a protein of 268 amino acid residues with a molecular mass of 30.07 kDa after SDS-PAGE analysis. The optimal temperature and pH of the purified enzyme were 45℃ and 7.5, respectively. The enzyme demonstrated good temperature tolerance, with 40% residual activity after 60 min of heat treatment at 75℃. The kinetic constants V_max and K_m of this nitrilase toward 3-cyanopyridine were 3.12 μmol/min/mg and 7.63 mM, respectively. Furthermore, this novel nitrilase exhibited a broad spectrum toward the hydrolysis of the aliphatic nitriles among the tested substrates, and particularly was specific to aliphatic dinitriles like succinonitrile, which was distinguished from most nitrilases ever reported. The catalytic efficiency k_cat/K_m was 0.44 /mM/s toward succinonitrile. This distinct characteristic might enable this nitrilase to be a potential candidate for industrial applications for biosynthesis of carboxylic acid.

• Korean Journal of Adult Nursing
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• v.19 no.4
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• pp.547-555
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• 2007

Purpose: The purpose of this study was to test a hypothetical model of health-related quality of life in patients with heart failure. The hypothetical model was derived from the Wilson and Cleary's model, the Rector's model, and published research findings. Methods: Data from 103 patients with heart failure were analyzed to determine the best multivariate health-related quality of life model given variables derived from the prior studies. The statistics programs SPSS 12.0 and LISREL 8.7 program were used for descriptive statistics and covariance structure analysis respectively. Results: The overall fitness of the path final model was good(GFI=.97, AGFI=.95, NNFI=1.06, NFI=.96, p=.96). Symptoms were directly affected by gender. HYHA Class was directly affected by only gender. Physical functioning limitation was directly affected by exercise. Health perception was directly affected by economics, symptom, and physical functioning limitation. Depression was directly affected by exercise and health perception. Heath-related quality of life was directly affected by physical functioning limitation and depression, indirectly affected by gender, economics, exercise, symptoms, NYHA Class, and health perception. This path analysis model explained 51% of health-related quality of life in patients with heart failure. Conclusion: To improve of health-related quality of life with heart failure patients, it is necessary to make nursing interventions for physical functioning and depression.

• Journal of agricultural medicine and community health
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• v.18 no.2
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• pp.173-183
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• 1993

This study reports the relationship between life-style and physical, mental health status in college students. The questionnaire survey was carried out from September to October, 1991. The questionnaire included life-style, physical health condition centered on unhealthy habits and complaints about physical symptoms, and mental condition which was assessed by Zung's self-rating depression scale(SDS). Days absent from school during the last year in males(3.6) were significantly more than those in females (1.0). Days catching the common cold were more in females than in males. The amount of complaints and the SDS score were significantly higher in females than in males. In the relationship between physical and mental health status and individual health habits, abscence-days from school significantly correlated with regular life, snacking, physical exercise, smoking, drinking. The amount of complaints and the SDS score were significantly associated with regular life, regular meal-time, eating breakfast, nutritional balance and physical exercise. In the relationship between health practice index to health status, the group having good habits showed significantly less days catching the common cold, less complaints, less SDS score and less amount of stress. It was also shown that life-style correlated with physical and mental health status.