• 한국산학기술학회논문지
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• 제10권12호
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• pp.3788-3796
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• 2009

본 연구는 심리학적 변인인 개인 감정구조를 도입하여 감정구조$\rightarrow$환경 몰입$\rightarrow$소비자유형(그린소비자 성향, 유사그린소비자 성향, 비그린소비자 성향)$\rightarrow$삶의 질에 관한 통합적 이론 모델을 구축하고자 실증분석을 하였다. 분석 결과는 다음과 같다. 첫째, 긍정적 감정은 환경몰입에 영향을 미치는 것으로 나타났으나, 부정적 감정은 환경몰입을 높이는 것으로 나타났으나 통계적으로 유의미하지 않았다. 둘째, 환경몰입은 그린소비자 성향, 유사그린소비자 성향 및 비그린소비자 성향에 영향을 미치는 것으로 나타났다. 마지막으로 그린소비자 성향은 삶의 질에 영향력을 미치는 것으로 나타났으나, 유사그린소비자 성향과 비그린소비자 성향이 삶의 질에 영향을 미치지 않는 것으로 나타났다.

• Journal of Microbiology and Biotechnology
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• 제20권7호
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• pp.1114-1120
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• 2010

A chitosan-degrading fungus, BSF114, was isolated from soil. The culture preparation showed strong chitosanolytic enzyme activity at an optimum pH of 4.0 and optimum temperature of $60^{\circ}C$ after 36-40 h fermentation. The rapid decrease in the viscosity of the chitosan solution early in the reaction suggested an endo-type cleavage of the polymeric chitosan chains. To identify the isolated fungus, molecular biological and morphological methods were used. The fungal internal transcribed spacer (ITS) region 1 was amplified, sequenced, and then compared with related sequences in the GenBank database using BLAST. The phylogenetic relationships were then analyzed, and the results showed that the fungus belongs to Aspergillus fumigatus. Morphological observations were also used to confirm the above conclusion. The chitooligosaccharides (COS) obtained through hydrolyzing the colloidal chitosan showed that A. fumigatus BSF114 is suitable for degrading chitosan and producing chitooligosaccharides on a large scale. High concentrations of the COS (1,000 and 500 ${\mu}g/ml$) significantly proliferated mice marrow cells.

• International Journal of Industrial Entomology and Biomaterials
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• 제23권1호
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• pp.147-153
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• 2011

The Pseudorabies (PR), also called Aujeszky's disease (AD), is an infectious viral disease caused by an alpha herpes virus and has domestic and wild pigs, as well as a wide range of domestic and wild animals, as the natural host. Pseudorabies virus (PRV) virions contain several envelope glycoproteins. Among them, gB, gC and gD are regarded as the major immunogenic proteins. We expressed these glycoproteins using the bacterial expression system and analyzed recombinant proteins. Expression of glycoproteins gC and gD were observed on SDS-PAGE or Western blot analysis, but gB was not. Optimal concentration of IPTG and inducing time were determined as 1.0 mM and 4 h, respectively, for the expression of both gC and gD in E. coli. A sodium dodecyl sulfate (SDS) was the most efficient detergent in solubilizing insoluble recombinant protein.

• 한국식생활문화학회지
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• 제30권5호
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• pp.621-628
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• 2015

The objective of the current study was to analyze the major factors affecting quality of life improvement for contract foodservice customers by identifying the contract foodservice environment consisting of the customer, foodservice management, and competitor. Qualitative research method was performed on foodservice customers and foodservice management using in-depth interviews. First, the customer environment was classified into three categories, including convenience of location, foodservice management environment into six categories, including comfort level of dining facility, and competitor environment into three categories, including service competition between foodservice providers. Second, quality of life was defined as the level of contentment felt by both the customer and foodservice management consuming the food provided. Third, both the customer and foodservice management perceived that the management environment of contract foodservice had a "medium" effect on quality of customer life. The findings of this study could be applicable for development of a contract foodservice business strategy through objective comparative analysis of the customer, foodservice management, and competitor environments.

• Mycobiology
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• 제45권3호
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• pp.204-208
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• 2017

Nosema ceranae is an obligate intracellular fungal parasite that causes mortality in honey bees and enhances the susceptibility of honey bees to other pathogens. Efficient purification of Nosema spores from the midgut of infected honey bees is very important because Nosema is non-culturable and only seasonably available. To achieve a higher yield of spores from honey bees, in this study, we considered that the initial release of spores from the midgut tissues was the most critical step. The use of 2 mm beads along with enzymatic treatment with collagenase and trypsin enhanced the homogenization of tissues and the yield of released spores by approximately 2.95 times compared with the use of common 3 mm beads alone. The optimal time for the enzyme treatment was determined to be 1 hr as measured by the yield and viability of the spores. A one-step filtration using a filter paper with an $8-11{\mu}m$ pore size was sufficient for removing cell debris. This method may be useful to purify not only N. ceranae spores but also other Nosema spp. spores.

• International Journal of Industrial Entomology and Biomaterials
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• 제22권2호
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• pp.75-82
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• 2011

To elucidate the novelty of Hyphantria cunea nucleopolyhedrovirus (HcNPV) isolated in Korea, polyhedrin and inhibitor of apoptosis (iap) gene structures were determined and analyzed. The analysis of HcNPV polyhedrin showed a little difference with 97.6% at the nucleotide level but no difference at the amino acid level when compared with that of previously reported H. cunea NPV (HycuNPV). On the other hand, iap genes showed variable differences with 89.0-99.6% nucleotide and 90.0-99.6% amino acid sequence identities. Especially, the 5' and 3' non-coding flanking sequences of iap1 gene had lower degree of identity with those of HycuNPV. Although the phylogenetic analyses using polyhedrin and iap genes showed that HcNPV is closely related with HycuNPV, we could provide that HcNPV is a novel isolate having novel gene structures.

• 전기전자학회논문지
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• 제24권4호
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• pp.1180-1183
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• 2020

최근 들어 IoT와 스마트홈의 발전에 따라 낙상 사고 감지, 화상 위험 감지와 같이 일상 생활에서의 안전 감지 기능이 많이 보급되기 시작했다. 이러한 안전 감지 기능은 대부분 인공지능에 의해 수행된다. 그러나 실험실 환경에서 안전 감지의 정확도만 평가하는 경우에는 실제로 일상 생활 환경에서 체감하게 되는 성능과 꽤 큰 차이를 보이는 경우가 많다. 본 논문에서는 이러한 문제점을 보완하기 위해 사용하는 두 가지 기법인 리빙랩과 혼동 매트리스를 소개한다. 리빙랩은 단순히 일상 생활환경의 모사를 넘어서 사용자가 직접 기술 개발 및 제품 설계에 참여할 수 있는 통로가 된다. 또한 혼동 매트리스에서 도출되는 다양한 성능 척도는 사용 목적에 적합하게 인공지능 시스템의 성능을 평가하는데 큰 도움을 준다.

• 가정과삶의질연구
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• 제28권5호
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• pp.53-66
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• 2010

The purpose of this study is to investigate the influence of family-friendly life environment factors including local community's living environment satisfaction (safety, convenience, comfort, accessibility, exchange of neighbor satisfaction), family support facility satisfaction, family support program satisfaction and nurture and education environment satisfaction, and local community's living information accessibility on the effect of multiple roles of married men and women with children. Data were collected from 380 married men and women with children located Seoul, GyeongGi-Do, ChungCheongBuk-Do, and KyungSangNam-Do. The results show that, first of all, related to general trend of role satisfaction and role strain, the subjects have higher experience of role satisfaction than role strain. Secondly, the variables of social demography, such as monthly income, non-regular full-time working system, white collar occupation, number of children and level of education, and living environment variables, such as satisfaction of living environment stability and satisfaction of neighbor exchange in living environment were significantly related to role strain. Thirdly, social demography variables, such as average monthly earnings, gender (male), non-regular part-time working system, satisfaction of stability in living environment, and satisfaction of convenience in living environment were significantly related to role strain.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2003년도 추계 학술발표회 요지
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• pp.58-59
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• 2003

• 한국수자원학회:학술대회논문집
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• 한국수자원학회 2005년도 학술발표회(2)
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• pp.1378-1378
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• 2005