• Molecules and Cells
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• v.43 no.8
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• pp.694-704
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• 2020

HslUV is a bacterial heat shock protein complex consisting of the AAA+ ATPase component HslU and the protease component HslV. HslV is a threonine (Thr) protease employing the N-terminal Thr residue in the mature protein as the catalytic residue. To date, HslUV from Gram-negative bacteria has been extensively studied. However, the mechanisms of action and activation of HslUV from Gram-positive bacteria, which have an additional N-terminal sequence before the catalytic Thr residue, remain to be revealed. In this study, we determined the crystal structures of HslV from the Gram-positive bacterium Staphylococcus aureus with and without HslU in the crystallization conditions. The structural comparison suggested that a structural transition to the symmetric form of HslV was triggered by ATP-bound HslU. More importantly, the additional N-terminal sequence was cleaved in the presence of HslU and ATP, exposing the Thr9 residue at the N-terminus and activating the ATP-dependent protease activity. Further biochemical studies demonstrated that the exposed N-terminal Thr residue is critical for catalysis with binding to the symmetric HslU hexamer. Since eukaryotic proteasomes have a similar additional N-terminal sequence, our results will improve our understanding of the common molecular mechanisms for the activation of proteasomes.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.33 no.4
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• pp.388-395
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• 2009

An accelerated fatigue test is essentially required to maintain the reliability of the actual structures of KTX under operation conditions. However, actual fatigue life cannot be obtained because the conventional fatigue tests are not adequate to the real load conditions. Moreover foreign component makers have not provided data of the loading stresses (S) versus cycles at the failure (N). In this study, we suggested a deduction method of the S-N curve for establishing an accelerating test under various load levels. Load history was acquired from the field tests. A Rainflow method was used on the cycle counting of the field load data. After that, an S-N curve was obtained through the iteration process under the condition that the damage index satisfies to 1 in the Miner's rule. The deduced S-N curve was applied to the performance evaluation of Korean-made sealed knuckles compared with imports.

• Korean Journal of Food Science and Technology
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• v.21 no.6
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• pp.876-883
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• 1989

In order to investigate the index component and basis for the shelf-life of the grain share improved Meju, the effect of the storage time and package on the quality of Meju and soy sauce were studied during 90 days storage at $30^{\circ}C$. Also, sensory evaluation for the soy sauces from Meju with various storage time were carried out. During the storage period, moisture content, amylase and protease activity were decreased, on the other hand, the contents of amino-nitrogen and ammonia-nitrogen were increased. Among these components, protease activity was found the major index component for quality control because it was the most important component for soy sauce fermentation and the most changeable component of Meju during the storage period. According to the sensory test, the quality of soy sauce was agreed well with the protease activity of Meju, and the soy sauce from longer storage Meju was inferior organoleptic quality to that from shorter Meju. By the storage quality test, protease activity showed the highest value in 15 days storage Meju and decreased gradually with storage time passed. The basis of protease activity for quality control was 200 (O.D. at 660 nm/g). which was 50% of the initial activity And it was known that the shelf-life of the grain shape improved Meju was about 90 days . It was also shown that the storage in package did not affect noticeably to prolong the shelf-life of Meju on this study.

• The Korean Journal of Rehabilitation Nursing
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• v.17 no.1
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• pp.38-47
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• 2014

Purpose: This study was performed to investigate the effects of Tai Chi exercise on physical fitness and quality of life (QOL) in elderly women. Methods: This study was designed as a quasi-experimental, nonequivalent control group pre-post test design. The subjects were elderly women over 60 years old who enrolled senior citizen centers and elderly welfare center. Thirty-three people were conveniently assigned to an experimental group (n=15) or a control group (n=18). The data were collected from September 2009 to May 2010. The experimental group was trained Tai Chi exercise for 8 weeks (one hour Tai Chi classes twice a week and once a week by themselves at home). Data were analyzed by Fisher's exact test, ${\chi}^2$-test and independent t-test. Results: The experimental group experienced significant improvements in flexibility. The experimental group experienced significant improvements in physical component summary (p=.017) and mental component summary (p=.027) of QOL. Conclusion: Tai Chi exercise can be used as a nursing intervention for improvement of physical fitness and QOL in elderly women.

• Journal of Korea Society of Digital Industry and Information Management
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• v.8 no.3
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• pp.23-31
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• 2012

With the advance of Smart TV technologies, TV watchers can enjoy the Internet and games while watching TV programs. Besides retrieving with some keywords in the Internet, people may want to access local information such as notifications from town, messages from children's schools, shopping information from local marts, and even reminder messages for visiting from hospitals while watching TV without using web-browser. In this paper, we introduce the daily life notification service scenarios and its functional and non-functional requirements. Furthermore, we also propose a system that provides the notification services, consists of smart TV apps and server systems. We also introduce the system architecture and the component design of the system. Our work will help smart TV service developers because this paper will give them some service scenarios, requirements, and system architecture and its component design.

• International Journal of Industrial Entomology and Biomaterials
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• v.21 no.2
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• pp.157-162
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• 2010

Open reading frame 35 (bm35) of the Bombyx mori nucleopolyhedrovirus (BmNPV) is a special gene whose homologues are only found in some group-I nucleopolyhedroviruses, suggesting that bm35 plays a specific role in the viral life cycle. This paper described the characterization of BmNPV bm35. Computerassisted sequence analysis shows that a putative RING finger motif is observed in the protein, Bm35 encoded by bm35. The coding sequence of bm35 was amplified and subcloned into the vector pET30a(+) and the $(His)_6$-tagged fusion protein His-Bm35 was expressed in the Escherichia coli BL21 (DE3) LysS cells. The bm35 transcript and Bm35 protein were detected in BmNPV-infected BmN cells at 12~48 h post infection (p.i.) by RT-PCR and Western blot analysis using the polyclonal antibody generated by immunizing a rabbit with purified $(His)_6$-tagged Bm35, suggesting that bm35 is synthesized in the late stage of BmNPV infection cycle. Bm35 was not a structural component associated with budded virus (BV) and occlusion derived virus (ODV). These data indicated that bm35 is a functional gene in the BmNPV life cycle.

• International Journal of Reliability and Applications
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• v.2 no.4
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• pp.253-262
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• 2001

A k-out-of-n standby system is considered where all of its components are s-independent and classified either working or cold standby connected with imperfect switches. The probability density function of the life length for this system is established in closed form, when the underlying components have constant failure rates. Also the reliability function of the system is derived. Finally, the reliability functions for one, two and three out of four systems are deduced for perfect or imperfect switches and identical or non-identical constant failure rates for working and standby components.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.46 no.6
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• pp.717-724
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• 2013

To characterize the food components of two mussels (wild hard-shelled mussel (HM) Mytilus coruscus, and cultured sea mussel (SM) Mytilus edulis) in Korea, we examined the proximate composition, fatty composition, amino acid/mineral content, texture, collagen content and chemical and taste compounds. Regarding the proximate composition, HM had lower moisture levels and higher crude protein and carbohydrate contents than SM. The amino nitrogen, volatile basic nitrogen and total amino acid contents of HM and SM were 250.6 and 227.3 mg/100 g, 11.2 and 12.0 mg/100 g, and 17,451.1 and 15,334.8 mg/100 g, respectively. The major amino acids were glutamic acid, aspartic acid, glycine, alanine, lysine and arginine. The major fatty acids of HM and SM were 14:0, 16:0, 16:1n-7, 20:5n-3, and 22:6n-3, which did not differ significantly between the two mussels. HM had a higher n-3 polyene ratio, and a lower saturate and monoene ratio than SM. Regarding the taste-active compounds, the free amino acid contents of HM and SM were 1,116.5 and 961.8 mg/100 g, respectively, and the major free amino acids were taurine, glutamic acid, glutamine, glycine, citrulline, lysine and arginine. The primary minerals in both HM and SM were Na, Cl, K and P which did not differ significantly between the two mussels. The soluble and insolube collagen contents of HM and SM were 265.8 and 228.4 mg/100 g, and 119.5 and 121.8 mg/100 g, respectively.

• Proceedings of the KSME Conference
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• 2001.06a
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• pp.211-216
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• 2001

The components with the circular cross section have the symmetric combination parts for rotating balance and the crack emanates from the symmetric combination parts. The symmetric cracks from symmetric combination parts make a decrease in the component fatigue life more than single crack. In this study, to estimate the behavior of symmetric cracks, the fatigue test was performed using rotary bending tester on the specimen with a symmetric defects in circular cross section. The material used in this study is Ni-Cr-Mo steel alloy. Under the same stress, the result from the rotary bending fatigue test turned out that the symmetric cracks made a decrease in the fatigue life by 35% more than single crack and the relation between log a and cycle ratio $N/N_f$ obtained linearly.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.50 no.4
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• pp.453-457
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• 2017

The proximate, fatty acid and dimethyl acetal (DMA) composition, and mineral and total amino acid content of limpets Cellana spp. were studied. Limpets contained 17.6% protein, 1.82% lipids, and 1.28% ash. The most prominent fatty acids in limpets were 20:4n-6, 20:5n-3, 18:1n-9, 16:0, 18:0, and 22:2 nonmethylene-interrupted diene (7,13). Limpets contained significant amounts of 18:0DMA (7.79%) and 20:0DMA (2.45%) derived from plasmalogen. They also contained large amounts of calcium (192.4 mg/100 g) and iron (9.4 mg/100 g), which are components of limpet teeth. The most prominent total amino acids were glutamic acid, arginine, aspartic acid, leucine, lysine, and glycine. These results suggest that limpets are a good source of n-3 and n-6 fatty acids, plasmalogen, calcium iron, and arginine.