• 제목/요약/키워드: Life Stages

검색결과 1,605건 처리시간 0.032초

Effects of variation in the number and developmental stage of donor embryos and ovulation status of the surrogate mother on the efficiency of pig somatic cell cloning

  • Park, Mi-Ryung;Yoo, Jae Gyu;Hur, Chang-Gi;Sim, Bo-Woong;Kim, Myunghoo;Seo, Jakyeom;Kim, Byeong-Woo;Cho, Byung-Wook;Shin, Teak-Soon;Cho, Seong-Keun
    • 한국동물생명공학회지
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    • 제35권3호
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    • pp.258-264
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    • 2020
  • This study investigated the effect of variation in the number of somatic-cell-cloned embryos and their developmental stage at transfer on pregnancy, as well as the influence of the estrus status of recipient pigs on in vivo development of cloned porcine embryos after embryo transfer. For somatic cell nuclear transfer (SCNT), fibroblast cells were obtained from a male porcine fetus. Recipient oocytes were collected from prepubertal gilts at a local abattoir and then cultured. After SCNT, reconstructed embryos of different numbers and developmental stages were transferred into recipient pigs. The developmental stage of the cloned embryos and the number of transferred embryos per surrogate showed no significant differences in terms of the resulting cloning efficiency. However, the pregnancy rate improved gradually as the number of transferred cloned embryos was increased from 100-150 or 151-200 to 201-300 per recipient. In pre-, peri-, and post-ovulation stages, pregnancy rates of 28.6%, 41.8%, and 67.6% and 16, 52, and 74 offspring were recorded, respectively. The number of cloned embryos and estrus status of the recipient pig at the time of transfer of the cloned embryo affect the efficiency of pig production; therefore, these variables should be particularly considered in order to increase the efficiency of somatic cell pig cloning.

부부의 갈등과 생활만족도에 관한 연구 -형성기 가정과 확대기 가정을 중심으로- (A Study on Marital Conflict and Life Satisfaction -As Related to Establishment Family and Expanding Family-)

  • 김재경;문숙재
    • 가정과삶의질연구
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    • 제10권1호통권19호
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    • pp.53-74
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    • 1992
  • The purpose of this study was to analyse the factors that influence marital conflicts and family life statisfaction, and to predict the future stage of establishment family and extending family, bsed on the theretical model by system approach. For this pourpose, 336 couples of urban households in Seoul were selected. The data were analysed through statistical methods such as, Frequency, percentile, T-test, F-test, ANOVA, Pearson's correlation analysis. The results were as follow : The Martial Conflict and family life satisfaction of surveyed couples were above middle level. It had significantly differences according to family life cycle, husband and wife's educationed levels, husband and wife's occupations, and family income. And Family life satisfaction had significantly differences according to marital conflict. The marital conflict is higher, life satisfaction is lower. In this study, it were appeared that the amount of marital conflict and life satisfaction had differences according to life cycle stages, husband and wife's educational level, husband and wife occupations, and family income, and so that the conflict of phsycho-social system was more affected on family life satisfaction that of managerial system. Such results were indicated that the birth of children was the critical family event affecting on family life satisfaction on and the marital conflict. But the conflict by family's given resources and situational differences also as an environment affection on the family system.

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사구체 여과율에 따른 투석 전 만성 신부전 환자의 질병 관련 지식 정도와 교육 요구도 조사 (Study on Knowledge Levels of Pre-dialysis, Chronic Renal Failure Patients at Glomerular Filtration Rates (GFRs) and Their Educational Demands)

  • 김혜원;최스미
    • Journal of Korean Biological Nursing Science
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    • 제12권2호
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    • pp.114-126
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    • 2010
  • Purpose: To examine the levels of the disease-related knowledge and educational demands of pre-dialysis, chronic renal failure patients at different GFRs and stages. Methods: This study used a cross-sectional survey and aimed at 116 pre-dialysis, chronic renal failure patients, who were registered and received regular treatment from December 1 to December 31, 2009 at the nephrology departments of tertiary medical centers in Seoul. Stages were classified into Stage 3, 4 and 5 depending on GFR ranges. To measure the levels of knowledge and educational demands, the tool, which were first invented by Young Ae Lim (1996) and then modified by Hyo Sun Lim (2005) to adjust the knowledge and educational demands measuring tool for hemodialysis patients to pre-dialysis patients with chronic renal failure, was used. Collected data was analyzed with the SPSS WIN 12.0 program (average, standard deviation, Pearson's correlation coefficient, t-test and ANOVA). Results: There was a significant difference in the disease-related knowledge levels of the subjects at each stage (F=24.41, p=.000). The Scheffe post hoc test confirmed that patients at higher stages had higher levels of knowledge of their disease. Also, although the results showed that patients at higher stages had higher educational demands, there was no significant difference among the groups (F=1.259, p=.288). Conclusion: As patients have different levels of knowledge of the disease and educational demands depending on their stages, it is important to develop and use a systematic education program that reflects the demands and levels of patients at each stage in order to help pre-dialysis patients with chronic renal failure with self-management and improve their quality of life.

방문간호를 통한 암환자의 위기중재 효과에 관한 연구 (The Effect of Crisis Intervention by the Visiting Nurse with Patients with Cancer)

  • 김조자;유지수;박지원
    • 대한간호학회지
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    • 제19권1호
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    • pp.63-80
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    • 1989
  • This study was undertaken at Yonsei University Medical Center to identify the crisis responses and nursing problems of patients who had been diagnosed with cancer, and changing patterns of grieving over time periods, and to analyse the effectiveness of follow up care through home visiting nursing. This study was carried out in three stages. The 1st study data were collected from a total of 205 patients who had been diagnosed with cancer from Sept.1 to Dec. 31, 1987 using a cross-sectional method. The 2nd study data were collected three times from 30 patients with cancer at 4 weeks intervals from March 1 to June 31, 1988 using a longitudinal method. The 3rd study data were collected from two different groups from March 1 to June 31, 1988. One was an experimental group who was visited by nurses and the other one was a control group not visited by nurses. The subjects of the 3rd study consisted of 60 patients with cancer and a Quasi-experimental research design was used. The results were as follows ; 1. The patients did not experience one stage at a time among the five stages of grieing, denial anger, bargaining, depression and acceptance, as identified by Kubler Ross. They experienced a combination of stages, especially of the bargaining and the depression stages. This stages did not change with the passing of time. 2. The patients expressed more physical and socioecounomical problems than emotional problems. And they used more problem coping methods than emotional coping methods. 3. Follow up care through home visiting nursing positively influenced the patient's quality of life, especially their physical well-being and symptom control The patients responded positively to the home visiting nursing, stating that it was helpful In them. It was concluded that the development of a home visiting nursing program is needed for the effective home care of patients with cancer.

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일-가족 조화를 위한 가족친화적 기업정책의 발달단계에 관한 연구 (A Study on Stages of Development of Corporate Family-Friendly Policies and Programs)

  • 최성일;유계숙
    • 가정과삶의질연구
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    • 제24권4호
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    • pp.51-73
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    • 2006
  • The purpose of this study is to introduce the evolution of corporate family-friendly policies and programs, a recent business issue throughout the world. In addition, this study suggests implications for a corporate reference guide to work-family policies and programs by providing a comparative analysis of the typical stages of development of U.S. corporate family-friendly policies and programs, and by presenting model initiatives of Korean and American family-friendly companies. Just as technological changes at the time of the Industrial Revolution altered the relationship between workers and their work, more recent technological advances have again transformed this relationship, offering the promise of a society in which work demands a smaller portion of the worker's time and permitting the worker more freedom and control over work conditions. These new work arrangements have the promise of producing a new paradigm for work and family, based on which many industrialized nations have developed family-friendly policies already. Family-friendly policies and programs can be grouped into four discernable stages in the evolution of a corporate work-family agenda. According to these stages, most companies in Korea are in the predevelopment stage or stage 1. Development of scales to assess company family-friendliness is needed to create a family-friendly workplace environment, policies, and programs. It is critical that companies have champions who have the vision to step out in kent, and the determination to ensure that the family-friendly programs are solidly grounded. Companies should develop their work-family initiatives as an integral part of a program for managing diversity focusing on needs of women and minority employees.

생활체육참여자의 운동변화단계에 따른 운동자기도식과 운동중단유혹의 관계 (Relations between Exercise Self-Schema and Temptation of Quitting Exercise according to the Stages of Exercise Change among Participants in Sports for All)

  • 송기현;임현묵;김승용
    • 한국융합학회논문지
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    • 제10권4호
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    • pp.219-227
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    • 2019
  • 본 연구는 심리적 과정에 따른 운동행동의 변화를 알아보기 위해 운동변화단계와 운동자기도식, 운동중단유혹의 관계를 분석하는데 목적이 있다. 연구목적 달성을 위하여 공공스포츠센터를 이용하는 성인을 대상으로 총 297부의 최종자료를 사용하였다. 자료처리는 SPSS 18.0을 이용하여 탐색적 요인분석 후 다변량분석, 중다회귀분석, 판별분석을 통해 검증하였다. 연구결과는 첫째, 운동변화단계에 따라서 운동자기도식은 차이가 있는 것으로 나타났다. 둘째, 운동변화단계에 따라서 운동중단유혹은 지도자와 의욕상실에서 차이가 있는 것으로 나타났다. 셋째, 행동적 자기도식과 의욕상실은 운동변화단계를 판별하는데 주요 요인인 것으로 나타났다. 넷째, 운동자기도식은 의욕상실에 부적 영향을 미치는 것으로 나타났고, 감정에는 인지-감정적 자기도식이 부적 영향을 미치는 것으로 나타났다.

Experimental Life History and Biological Characteristics of Fasciola gigantica (Digenea: Fasciolidae)

  • Phalee, Anawat;Wongsawad, Chalobol;Rojanapaibul, Amnat;Chai, Jong-Yil
    • Parasites, Hosts and Diseases
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    • 제53권1호
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    • pp.59-64
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    • 2015
  • This study was conducted to investigate the life history, morphology, and maturation of larval stages and adult worms of Fasciola gigantica in experimental mice. Lymnaea auricularia rubiginosa was used as the intermediate host, and Oryza sativa was used for encystment of the metacercariae, while Mus musculus was used as the definitive host for maturation study. Fresh eggs from the gall bladder of water buffaloes fully developed into embryonated ones and hatched out at days 11-12 after incubation at about $29^{\circ}C$. Free-swimming miracidia rapidly penetrated into the snail host, and gradually developed into the next larval stages; sporocyst, redia, and daughter redia with cercariae. Fully-developed cercariae were separated from the redia and shed from the snails on day 39 post-infection (PI). Free-swimming cercariae were immediately allowed to adhere to rice plants, and capsules were constructed to protect metacercariae on rice plants. Juvenile worms were detected in intestines of mice at days 3 and 6 PI, but they were found in the bile duct from day 9 PI. Juvenile and adult flukes were recovered from 16 mice experimentally infected with metacercariae, with the average recovery rate of 35.8%. Sexually mature adult flukes were recovered from day 42 PI. It could be confirmed that experimentally encysted metacercariae could infect and develop to maturity in the experimental host. The present study reports for the first time the complete life history of F. gigantica by an experimental study in Thailand. The obtained information can be used as a guide for prevention, elimination, and treatment of F. gigantica at environment and in other hosts.

Characterization of EST Gene in the Bovine Corpus Luteum during the Estrous Cycle

  • Lee, Eunyoung;Kim, Sang Hwan;Kim, Byung-Gak;Yoon, Jong Taek
    • 한국발생생물학회지:발생과생식
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    • 제19권4호
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    • pp.227-234
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    • 2015
  • The objective of this study was to investigate the expression of bovine luteum expressed sequence tags (ESTs), vascular endothelial growth factor (VEGF), and tumor necrosis factor receptor 1 (TNFR1) and the presence of functional ESTs in the bovine corpus luteum (CL) during different stages of the estrus cycle. Reverse transcription-polymerase chain reaction (RT-PCR) analysis showed a difference in the expression of ESTs during the CL stage. Concentration of ESTs in the CL tissue increased significantly from the mid-luteal stage and decreased thereafter. RT-PCR analysis showed higher levels of the EST genes in the CL of the mid-luteal stage than in other stages, and the same level of expression of VEGF. Immunohistochemistry analysis of the tissue from CL formation to regression showed low cytosol and aggregation of the nucleus. And activity caspase 3 (apoptosis detector) was most strongly detected in the CL1 stage of bovine. During the estrous cycle, the cytosol was magnified and differentiation of the nucleus was clearly manifested. The ESTs affected the CL, and the relationship between VEGF and TNFR1 played a pivotal role for CL development and activation, dependent on the stage of CL. These results suggest local production of ESTs, the presence of functional ESTs in the bovine CL, and that ESTs play a role in regulating the function of cell death in bovine CL.

배합사료 크기가 성장단계별 넙치(Paralichthys olivaceus) 성장에 미치는 영향 (Effect of Extruded Pellet Size on Growth of Olive Flounder Paralichthys olivaceus at Three Different Growing Stages)

  • 김민기;신재형;이초롱;이봉주;김강웅;이경준
    • 한국수산과학회지
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    • 제52권1호
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    • pp.43-48
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    • 2019
  • This study was conducted to investigate the effect of extruded pellet (EP) size on the growth performance of olive flounder Paralichthys olivaceus at the juvenile (Exp-I; $62.2{\pm}0.05$), growing (Exp-II; $150{\pm}1$) and sub-adult (Exp-III; $299{\pm}1$) stages. In Exp-I (3, 4, 5 and 6 mm diets), Exp-II (4, 9, 11 and 13 mm diets) and Exp-III (9, 11, 13 and 16 mm diets), triplicate groups of fish were fed four EP diets of different pellet sizes. In Exp-I, larger pellet sizes were significantly better than the smallest pellet size (3 mm). In Exp-II, significantly improved feed and protein efficiency were observed with increasing pellet size. The feed conversion ratios of the fish fed the 4 and 9 mm diets were significantly lower than those of fish fed 11 and 13 mm diets. The dietary digestibility of protein and energy was higher in smaller EPs than in larger sizes. In Exp-III, the growth performance of fish was not affected by the pellet size. However, feed intake was significantly affected, increasing with increasing pellet size. Therefore, the optimum pellet sizes for olive flounder under the conditions in this study were 5 mm for 60-150 g fish and 9 mm for 150-500 g fish.

패션 소상공인 제품 구매에 대한 탐색적 연구 -소비자 생애주기와 구매단계를 중심으로- (Exploratory Study on Purchasing Fashion Products from Small Business Owners -Focusing on the Consumer Life Cycle and Purchasing Stage-)

  • 김송미;장세윤;이유리;진우준;김하연
    • 한국의류학회지
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    • 제46권5호
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    • pp.805-826
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    • 2022
  • This study explored the process by which consumers purchase products from small fashion business owners via online and mobile channels. In addition, group types were classified given that the purchasing process depends on the consumers' life cycle. The consumer focus group interview (FGI) was conducted on 18 participants that were divided into six groups by age, work, and children. Results revealed that first, consumer journey comprised four stages. Factors influencing need recognition were "attention to information of social media influencer," "attention to information of affiliated groups," and "repeated advertising of SME products/brands." For information searching, "exploring purchase reviews," "environment for mobile shopping information exploration," and "continuous product tracking" were important factors. Purchasing and shopping stages were affected by "price-free, improvised purchase decision" and "convenient mobile payment system and point benefits." After the purchase, "active sharing and repeated purchase when satisfied" and "blocking relationships when dissatisfied" occurred. Second, six consumer groups based on the fashion life cycle are the "Platform lover," "Influencer follower," "Trust builder," "Novelty seeker," "Convenience seeker," and "New designer supporter." Ultimately, small business owners can develop the process of planning and selling fashion products more efficiently.