• 한국수정란이식학회지
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• 제24권1호
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• pp.21-27
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• 2009

This study was performed to elucidate the effects of addition of ${\beta}-lactoglobulin$ and bovine serum albumin (BSA) in vitro maturation (IVM) and in vitro culture (IVC) medium on porcine embryo production. The development rate to the 2 cell ($71.4{\sim}75.6%$) and blastocyst stages ($6.8{\sim}13.3%$) with different BSA concentrations in IVM medium were similar among treatment groups. Blastocyst hatching rate was significantly higher in the control group (0.0mg/ml) than in the group of 1.0mg/ml supplement (20.0% vs. 0.0%; p<0.05). The development rate to the 2 cell ($62.0{\sim}70.6%$) and blastocyst stages ($15.4{\sim}38.5%$) with different ${\beta}-lactoglobulin$ concentrations in IVM medium was similar among treatment groups. The development rate to the blastocyst was significantly higher in the group of 1.0mg/ml(15.3%) than in the group of 0.5mg/ml supplement (7.6%, p<0.05). The development rate to the 2 cell and blastocyst stages following the first addition of ${\beta}-lactoglobulin$ in IVM medium was significantly higher in the control group (77.0% and 18.9%) and was $0{\sim}44\;hr$(77.2% and 16.9%) greater than that observed in other treatment groups (p<0.05). The development rate to the 2 cell stage ($68.1{\sim}74.8%$) and blastocyst stages ($9.2{\sim}12.7%$) with different BSA concentrations in IVC medium was similar among treatment groups. However, blastocyst hatching rate was significantly higher in the group of 3.0mg/ml supplement (30.0%) than in the control group (0.0%; p<0.05). The development rate to the 2 cell stage ($72.9{\sim}78.0%$), blastocyst ($7.1{\sim}14.2%$) and hatching stages ($33.3{\sim}38.1%$) were not different. The development rate to the 2 cell stage ($63.6{\sim}72.5%$), blastocyst ($8.4{\sim}16.1%$) and hatching stages ($18.2{\sim}37.5%$) at the different culture periods were similar among treatment groups. This study suggested that if the addition level and periods of ${\beta}-lactoglobulin$ addition are adjusted, it is possible to replace BSA in the in vitro porcine embryo production.

• 대한환경공학회지
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• 제34권4호
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• pp.270-279
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• 2012

본 연구에서는 남극 장보고기지 건설 시 발생하는 온실가스 배출량을 자재생산단계와 시공단계로 구분하여 산정하였다. 또한, 시공단계의 배출원은 각각 해양수송과 내륙수송, 건설장비 사용, 건설캠프 운영으로 분류하여 온실가스 배출량을 구체적으로 산정하였다. 전과정평가 개념을 도입해 남극 장보고기지 건설에 투입되는 자재생산을 통해 배출되는 온실가스 배출량은 기지 건설에 따른 전체 온실가스 배출량의 23.8%에 해당하는 8,933 ton (as $CO_{2eq}$)으로 산정되었으며, 향후 남극 신규기지의 건설 시 자재생산을 통한 온실가스 배출이 반드시 고려해야 할 대상인 것으로 판단되었다. 남극 장보고기지 시공단계(총 2단계)에서의 온실가스 배출량은 1단계에 비해서 2단계에 비교적 많이 배출되었으며, 이는 2단계 때 수송화물선이 부산까지 회항하여 정박하므로 연료사용량이 증가하기 때문인 것으로 사료된다. 또한, 시공단계의 온실가스 배출원 가운데 해양수송이 내륙수송, 건설장비 사용 및 건설캠프 운영에 비해 다량의 온실가스를 배출하는 것으로 판명되었다. 남극 장보고기지 건설에 따른 자재생산단계와 시공단계를 통합한 전체 온실가스 배출량은 총 34,486 ton (as $CO_{2eq}$)으로 기존 남극기지 포괄적 환경영향평가(CEE)상에 제시된 온실가스 총배출량과 비교 시 다량의 온실가스가 배출되는 것으로 나타났다. 이는 기존 CEE상에 제시된 온실가스 배출량 산정 시 건설에 투입되는 자재생산을 통해 발생하는 온실가스 배출량 산정이 고려되지 않고, 단순히 자재수송 및 시공단계만을 고려하여 온실가스 배출량을 개략적으로 산정하였기 때문으로 판단된다.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2001년도 춘계학술대회 논문집
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• pp.358-362
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• 2001

Environmental Product Life Cycle Management is an activity for defining and describing the product, process of activity environmentally. The activity involves the full life cycle stages of the product; evaluating environmental releases at each stage, determining the aggregate and specific impacts of the releases, developing opportunities to effect environmental improvements. This paper presents a methodological approach for database modeling to build Product Life Cycle Management system and show a set of database modeling. Additionally, a key issue for database is the quality of the provided information.

• 한국컴퓨터정보학회논문지
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• 제22권3호
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• pp.69-79
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• 2017

In this paper, we propose our four-phase life cycle of P2P botnet with corresponding detection methods and the future direction for more effective P2P botnet detection. Our proposals are based on the intensive analysis that compares existing P2P botnet detection schemes in different points of view such as life cycle of P2P botnet, machine learning methods for data mining based detection, composition of data sets, and performance matrix. Our proposed life cycle model composed of linear sequence stages suggests to utilize features in the vulnerable phase rather than the entire life cycle. In addition, we suggest the hybrid detection scheme with data mining based method and our proposed life cycle, and present the improved composition of experimental data sets through analysing the limitations of previous works.

• 가정과삶의질연구
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• 제14권3호
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• pp.167-180
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• 1996

The purpose of this study was to examine the differences in the rural housewives' resourcs and demands financial practices and financial satisfaction according to family life cycle and to analyze the causal relationships among the components. For the latter purpose the conceptual framework based on the system theory was suggested. The results showed that first most variables were significantly different according to the family life cycle. Second except the establishing stage the conceptual framework was supported in all stages. Variables which affected financial practices and financial satisfaction varied with the family life cycle.

• 가족자원경영과 정책
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• 제1권1호
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• pp.27-42
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• 1997

The purpose of this study is to analyze consumption expenditure patterns over family life cycle. The data used in the study is the 1994 Survey data from Annual Report on the Family Income and Expenditure Survey. The study sample included 26,980 salary and wage earners’ households living in cities. The family life cycle was classified into six stages and items of expenditure were classified into 12 categories. Frequency distribution, mean, one-way ANOVA, Scheff test, and regression analysis were used to analyze the data. The major findings of this study are as follows : First, the average consumption propensity differs among family life cycle. Second, the amount and budget share of consumption expenditure for each expenditure category differ significantly among family life cycle in all expenditure categories. Third, family life cycle is found to be a significant factor on expenditure of food and education, and also on budget share of education.

• 한국자원식물학회지
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• 제19권3호
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• pp.440-446
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• 2006

To investigate flowering related genes in winter-type oilseed rape (Brassica napus L. cv. Tammi), differentially expressed genes were isolated from leaves of the plant after low temperature treatment which is requirements for floral induction. As a result of suppression subtractive hybridization (SSH), 288 clones were randomly selected from SSH library. Using reverse Northern blot analysis, 150 of 288 clones were identified to be differentially expressed. Out of these 150 clones, 45 clones showed very high identities with the known genes. Four clones showed very high identities over 90% with metallothionein-like gene that is related to flowering-induced genes. Of these 4 clones, the cDNA clone, rfs-13, revealed high identity with meotallothionein-like protein in Arabidopsis thaliana (98%) and Brassica compestris (89%). Furthermore, gene expressed in immature flower stages was confirmed by Northern blot analysis.

• 가정과삶의질연구
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• 제35권1호
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• pp.1-16
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• 2017

Using data from the 2015 Korea Welfare Panel Study, we examined the predictors of psychological well-being (life satisfaction and depression) of single elderly people living alone across development stages (young-old, mid-old, and old-old) and suggested implications for elderly policy. For this purpose, we selected 1,364 elderly people who lived alone but preserved their family relationships, and were 65 years of age or older. Separate analyses were conducted for the three groups of single elderly people living alone. Overall, the psychological well-being of single elderly people living alone was higher than moderate level, and they were generally satisfied with their everyday lives and less depressed. However, there were group differences: the young-old group was more satisfied than the old-old group with in their everyday lives and less depressed than the mid-old and old-old groups. In the young-old group, life satisfaction was significantly associated with subjective health, non-physical conflict with family, disposal income, house ownership, dietary deficiency, cost of living, and public pension. In addition, depression was significantly associated with subjective health, non-physical conflict with family, dietary deficiency, and physical conflict with family. In the mid-old group, life satisfaction was significantly associated with subjective health, housing non-physical conflict with family, disposal income, and dietary deficiency. Depression was significantly associated with subjective health, housing deficiency. In the old-old group, life satisfaction was significantly associated with subjective health and non-physical conflict with family. Depression was significantly associated with subjective health. Therefore, 'health'and 'family'are important key concepts to consider when making elderly policy.

• Journal of Plant Biotechnology
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• 제48권1호
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• pp.12-17
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• 2021

Saponarin found in young barley sprouts has a variety of beneficial biological and pharmacological properties, including antioxidant, hypoglycemic, antimicrobial, and hepatoprotective activities. Our previous work demonstrated that saponarin content was correlated with the expression levels of three biosynthetic pathway genes [chalcone synthase (HvCHS1), chalcone isomerase (HvCHI), and UDP-Glc:isovitexin 7-O-glucosyltransferase (HvOGT1)] in young barley seedlings under various abiotic stress conditions. In this study, we investigated the saponarin content and expression levels of three saponarin biosynthetic pathway genes in hulled and hulless domestic barley cultivars. In the early developmental stages, some hulled barley cultivars (Kunalbori1 and Heukdahyang) had much higher saponarin contents than did the hulless barley cultivars. An RNA expression analysis showed that in most barley cultivars, decreased saponarin content correlated with reduced expression of HvCHS1 and HvCHI, but not HvOGT1. Heat map analysis revealed both specific increases in HvCHS1 expression in certain hulled and hulless barley cultivars, as well as general changes that occurred during the different developmental stages of each barley cultivar. In summary, our results provide a molecular genetic basis for the metabolic engineering of barley plants to enhance their saponarin content.

• 한국수산과학회지
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• 제57권1호
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• pp.41-52
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• 2024

Serotransferrin cDNA from the Siberian sturgeon Acipenser baerii was isolated and its expression patterns during early life intervals were characterized. It contained an ORF encoding a 708-aa-long polypeptide, including a 19-aa signal peptide. Bioinfomatic analysis and 3D modeling indicated a typical bi-lobal structure with conserved iron-coordinating residues. During embryonic development, the potential transition of maternally provisioned transcripts to zygotically de novo transcribed ones occurred around blastula stage. The transferrin mRNA levels peaked at stages responsible for pronephros, heart and erythropoietic component differentiation. After hatching, the transferrin mRNA expression gradually increased at early ontogenic phases (0 to 3 DPH) corresponding to the periods in which prolarvae exhibited increased blood circulation and liver differentiation. The expression decreased at subsequent stages in which prolarvae exhibited benthic movement. The tissue distribution assay indicated liver-predominant expression at fingerling stage. From the microinjection-based challenge with Aeromonas hydrophila at day-0 and day-7, the transcriptional response was modulated toward upregulation, in which the amounts induced at 6, 12 and 24 HPI were greater in prolarvae injected at day-7 than at day-0. Therefore, transferrin plays important roles in both early development and host protective responses to pathogens in the Siberian sturgeon.