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Effects of the Addition of ${\beta}-lactoglobulin$ and BSA on the Development of Porcine Embryos  

Park, Yong-Soo (Gyeongbuk Livestock Research Institute)
Kim, Myoung-Sin (Division of Life Food and Biotech., Daegu University)
Park, Hum-Dae (Division of Life Food and Biotech., Daegu University)
Publication Information
Journal of Embryo Transfer / v.24, no.1, 2009 , pp. 21-27 More about this Journal
Abstract
This study was performed to elucidate the effects of addition of ${\beta}-lactoglobulin$ and bovine serum albumin (BSA) in vitro maturation (IVM) and in vitro culture (IVC) medium on porcine embryo production. The development rate to the 2 cell ($71.4{\sim}75.6%$) and blastocyst stages ($6.8{\sim}13.3%$) with different BSA concentrations in IVM medium were similar among treatment groups. Blastocyst hatching rate was significantly higher in the control group (0.0mg/ml) than in the group of 1.0mg/ml supplement (20.0% vs. 0.0%; p<0.05). The development rate to the 2 cell ($62.0{\sim}70.6%$) and blastocyst stages ($15.4{\sim}38.5%$) with different ${\beta}-lactoglobulin$ concentrations in IVM medium was similar among treatment groups. The development rate to the blastocyst was significantly higher in the group of 1.0mg/ml(15.3%) than in the group of 0.5mg/ml supplement (7.6%, p<0.05). The development rate to the 2 cell and blastocyst stages following the first addition of ${\beta}-lactoglobulin$ in IVM medium was significantly higher in the control group (77.0% and 18.9%) and was $0{\sim}44\;hr$(77.2% and 16.9%) greater than that observed in other treatment groups (p<0.05). The development rate to the 2 cell stage ($68.1{\sim}74.8%$) and blastocyst stages ($9.2{\sim}12.7%$) with different BSA concentrations in IVC medium was similar among treatment groups. However, blastocyst hatching rate was significantly higher in the group of 3.0mg/ml supplement (30.0%) than in the control group (0.0%; p<0.05). The development rate to the 2 cell stage ($72.9{\sim}78.0%$), blastocyst ($7.1{\sim}14.2%$) and hatching stages ($33.3{\sim}38.1%$) were not different. The development rate to the 2 cell stage ($63.6{\sim}72.5%$), blastocyst ($8.4{\sim}16.1%$) and hatching stages ($18.2{\sim}37.5%$) at the different culture periods were similar among treatment groups. This study suggested that if the addition level and periods of ${\beta}-lactoglobulin$ addition are adjusted, it is possible to replace BSA in the in vitro porcine embryo production.
Keywords
porcine; BSA; ${\beta}-lactoglobulin$; in vitro culture;
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1 Camous S, Heyman Y, Menezo W and Menezo Y. 1984. Cleavage beyong the block stage and survival after transfer of early bovine embyos cultured with trophoblastic vesicles. J. Reprod. Fertil. 72:479-485   DOI   PUBMED   ScienceOn
2 Kane MJ. 1987. Minimal nutrient requirements for culture of one-cell rabbit embryos. BioI. Reprod. 37:775-778   DOI   PUBMED   ScienceOn
3 Motlik J, Grozet Nand Fulka J. 1984. Meiotic competence in vitro of pig oocytes isolated from early antral follicles. J. Reprod. Fertil. 72:323-328   DOI   PUBMED   ScienceOn
4 Petters RM and Wells KD. 1993. Culture of pig embryos. J Reprod. Fertil. Suppl. 48:61-73   PUBMED
5 Sommer P, Rath D and Niemann H. 1992. In vitro maturation of porcine oocytes in the presence of follicular granulosa cells, FSH and/or EGF. Proc. 12th Int. Cong. Anim. Repord. 1:378-380
6 Yoshida M, Mizoguchi Y, Ishigaki K, Kojima T and Nagai T. 1993. Birth of piglets derived from in vitro fertilization of pig oocytes matured in vitro. Theriogenology 39:1303-1311   DOI   ScienceOn
7 Gardner DK. 1994. Mammalian embryo culture in the absence of serum or somatic cell suppot. Cell. Int. 18:1163-1179   DOI   PUBMED   ScienceOn
8 Mattioli M, Bacci ML, Galeati G and Seren E. 1989. Developmental competence of pig oocytes matured and fertilized in vitro. Theriogenology 31:95-105   DOI   ScienceOn
9 Reed ML, Estrada JL, Illera MJ and Petters RM. 1993. Effects of epidermal growth factor, insulinlike growth factor-I and dialyzed porcine follicular fluid on porcine oocyte maturation in vitro. J. Exp. Zool. 266:74-78   DOI   ScienceOn
10 Iritani A, Niwa K and Imai H. 1978. Sperm penetration in vitro of pig follicular oocytes matured in culture. J. Reprod. Fertil. 54:384-394   DOI   ScienceOn
11 Abeydeera LR, Wang WH, Cantley TC, Rieke A, Murphy CN and Day BN. 2000. Development and viability of pig oocytes matured in a protein-free medium containing epidermal growth factor. Theriogenology 54:787-797   DOI   ScienceOn
12 Marchal R, Feugang JM, Perreau C, Venturi E, Terqui M and Mermillod P. 2001. Meiotic and developmental competence of prepubertal and adult swine oocytes. Theriogenology 56:17-29   DOI   ScienceOn
13 Edwards RG. 1965. Maturation of mouse, sheep, cow, pig, rhesus monkey and human ovarian oocytes. Nature 208:349-352   DOI   PUBMED   ScienceOn
14 Anbari K and Schultz RM. 1993. Effect of sodium and betaine in culture media on development and relative rates of protein synthesis in reimplantation mouse embryo in vitro. Mol. Reprod. Dev. 35:24-28   DOI   ScienceOn
15 Cholewa JA and Whitten WK. 1970. Development of two-cell mouse embryos in the absence of a fixed-nitrogen source. J. Reprod. Fert. 22:553-555   DOI   ScienceOn
16 Legge M and Sellens MH. 1991. Free radical sacvengers ameliorate the 2-cell block in mouse embryo culture. Hum. Reprod. 6:867-871   PUBMED
17 Singh B, Barbe GJ and Armstrong DT. 1993. Factors influencing resumption of meiotic maturation and cumulus expansion of porcine oocyte-cumulus cell complexes in vitro. Mol. Repord. Dev. 36:113-119   DOI   ScienceOn
18 Abeydeera LR, Wang WH, Prather RS and Day BN. 2001. Effect of incubation temperature on vitro maturation of porcine oocytes: Nuclear maturation, fertilization and developmental competence. Zygote 9:331-337   PUBMED
19 Day BN, Abeydeera LR, Johnson LA, Welch GR, Wang WH, Boquest AC, Cantley TC and Rieke A. 1998. Birth of piglets preselected for gender following in vitro fertilization of in vitro matured pig oocytes by X and Y chromosome bearing spermatozoa stored by high speed flow cytometry. Theriogenology 50:981-988   DOI   ScienceOn
20 Funahashi H and Day BN. 1993. Effects of the duration of exposure to hormone supplements on cytoplasmic maturation of pig oocytes in vitro. J. Reprod. Fertil. 98:179-185   DOI   PUBMED   ScienceOn
21 Heyman Y, Menezo Y, Chesne P, Camous S and Gardner V. 1987. In vitro cleavage of bovine and ovine early embryos: Improved development using co-culture with trophoblastic vesicles. Theriogenology 27:59-68   DOI   ScienceOn
22 Rho SH and Hwang WS. 2002. In vitro development of porcine parthenogenetic and cloned embryos: comparison of oocytes-activating technique,various culture systems and nuclear transfer methods. Reprod. Fertil. Deva. 14:93-99   DOI   ScienceOn
23 Motlik J and Fulka J. 1974. Fertilization of pig follicular oocytes cultivated in vitro. J. Reprod. Fertility. 36:235-237   DOI   ScienceOn
24 Yoshika K, Suzuki C, Tanaka A, Anas IM and Iwamura S. 2002. Birth of piglets derived from porcine zygotes cultured in a chemically defined medium. Biol. Reprod. 66: 112-119   DOI   ScienceOn
25 Biggers JD, Summers MC and McGinnis LK. 1997. Polyvinyl alcohol and amino acids as substitutes for bovine serum albumin in culture media for mouse preimplantation embryos. Hum. Reprod. Update. 3:125-135   DOI   ScienceOn
26 Kricher RL, Lane M and Bavister BD. 1999. Developmental competence and metabolism of bovine embryos cultured in semi-defined culture media. BioI. Reprod. 60:1345-1352   DOI   ScienceOn
27 Ka HH， Sawai K, Wang WH, Im KS and Niwa K. 1997. Animo acids in maturation medium and presence of cumulus cells at fertilization promote male pronuclear formation in porcine oocytes matured and penetrated in vitro. BioI. Reprod. 57:1478-1483   DOI   ScienceOn
28 Leibfried-Rutledge ML, Crister ES and First NL. 1986. Effect of fetal calf serum and bovine serum albumin on in vitro maturation and ferilization of bovine and hamster cumulus oocyte complexes. Biol. Reprod. 35:850-857   DOI   ScienceOn
29 Illera MJ, Loren PL, Illera JC and Petters RM. 1998. Developmental competence of immature pig oocytes under the influence of IVM-IVF processes. Journal of Developmental Biology 42:1169-1172
30 Edwards LJ, Williams DA and Gardner DK. 1998. Intracellular pH of the mouse preimplantation embryo: amino acids act as buffer of intracellular pH. Hum. Reprod. 13:3441-3448   DOI   ScienceOn
31 Naito K, Fukuda Y and Toyoda Y. 1988. Effects of porcine follicular fluid on male pronucleus formation in porcine oocyte matured in vitro. Gamete, Res. 21:289-295   DOI
32 Fukui Y, Terawaki Y and Ono H. 1992. Effect of gonadotropins and steroids and culture on bovine oocytes maturation. Theriogenology 18:161-175   DOI   ScienceOn
33 Abeydeera LR and Day BN. 1997. Fertilization and sub-sequent development in vitro of pig oocytes inseminated in a modified Tris-buffered medium with frozen-thawed ejaculated spermatozoa. BioI. Reprod. 57:729-734   DOI   ScienceOn
34 Ding J and Foxcroft GR. 1994. Epidermal growth factor enhances oocyte maturation in pigs. Mol. Reprod. Dev. 39: 30-40   DOI   ScienceOn
35 Lee ES and Fukui Y. 1996. Synergistic effect of alanin and glycine on bovine embryos cultured in a chemically defined medium and animo acid uptake by in vitro produced bovine morulae and blastocyst. Biol Reprod. 55:1383-1389   DOI   ScienceOn