• BMB Reports
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• v.42 no.11
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• pp.737-742
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• 2009

Hypoxia-inducible factor-$1{\alpha}/{\beta}$ (HIF-$1{\alpha}/{\beta}$) is a heterodimeric transcriptional activator that mediates gene expression in response to hypoxia. HIF-$1{\alpha}$ has been noted as an effective therapeutic target for ischemic diseases such as myocardiac infarction, stroke and cancer. By using a yeast two-hybrid system and a random peptide library, we found a 16-mer peptide named F29 that directly interacts with the bHLH-PAS domain of HIF-$1{\alpha}$. We found that F29 facilitates the interaction of the HIF-$1{\alpha/\beta}$ heterodimer with its target DNA sequence, hypoxia-responsive element (HRE). The transient transfection of an F29-expressing plasmid increases the expression of both an HRE-driven luciferase gene and the endogenous HIF-1 target gene, vascular endothelial growth factor (VEGF). Taken together, we conclude that F29 increases the DNA-binding ability of HIF-$1{\alpha}$, leading to increased expression of its target gene VEGF. Our results suggest that F29 can be a lead compound that directly targets HIF-$1{\alpha}$ and increases its activity.

• Journal of Korean Library and Information Science Society
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• v.18
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• pp.91-138
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• 1991

The paper describes the application areas of the hypertext and the relevance of hypertext principles to the information retrieval system. As to the techniques of the hypertext information retrieval the various navigation method including a guided tour, a history list, a browser, a book-mark, etc. are discussed. The query system is considered as the other technique to be integrated into the hypertext system for the enhancement of the interactive function of the information retrieval. Based on the theoretical background, a conceptual model of hypertext information retrieval system was constructed using GUIDE which was developed by P.J. Brown of Kent University. 16 bibliographic records from LISA of 1991(June) were used for the illustration of the basic operation of the system. Though the study could not reach the implementation level due to the absolute constraints of the time and experimental environments, further efforts will continue to develop a prototype system of a hypertext information retrieval. A few conclusions can be derived from the study : (1) The integration of the hypertext into the information retrieval system can be justified by permitting the end-users to have much stronger and more flexible interaction with the system. (2) The more the degree of the sofistication of the existing information retrieval system is the more the possibility of the development of an effective and user-oriented information retrieval system will be greater by integrating guide as a front end system to the underlying software. (3) The deep knowledge about the functions of information retrieval which can be enhanced by the hypertext could be acquired by the information retrieval specialists.

• The Journal of Asian Finance, Economics and Business
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• v.1 no.3
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• pp.37-42
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• 2014

The objective of the study is to find out the faculty members' perspective of most and least preferred parameters affecting the quality of education in an affiliated undergraduate engineering institution in Haryana, India. It is a descriptive research. The data has been collected with the help of Questionnaire Based Survey. The sample size for the study is 110. The respondents are the faculty members teaching B. Tech who were selected randomly from the above said geographical area. For data analysis and conclusion of the results of the survey, statistical tool like factor analysis was performed in SPSS. The most preferred aspects of the institution by the faculty members are: a secured Wi-Fi facility is well channelized to provide easy access, library is well equipped, faculty can visit the library with ease whenever they find time, toilets for the faculty are hygienic and in adequate number, parking facility for the faculty vehicle is spacious, adequate Industry Institute Interaction for the faculty development etc. The least preferred aspects of the institution by the faculty members are: faculty / staff rooms are spacious, well furnished and adequate in number, and working relationships between the Head of Departments and their faculty members are synchronized.

• Journal of the Korean Society for Library and Information Science
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• v.33 no.1
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• pp.49-65
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• 1999

The purpose of this study was to examine the impacts of individual factors, such as demographic characteristics, personality types and cognitive styles, on job satisfaction of university librarians. The study further investigated the relationships and interaction effects among those factors. Three independent variables examined were demographic characteristics (sex, age, year at work, and position), personality types (Type A and Type B), and cognitive styles (Held-dependence and Held-independence). The dependent varilable was the level of job satisfaction. five factors that affect the level of job satisfaction were the nature of work, salary level promotion practices, supervision style, and the relationships with co-wokers. The number of subjects participated in the study was 166 university librarians working in Pusan area. The SPSSWIN package was used for statistical analysis.

• Journal of the Korean BIBLIA Society for library and Information Science
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• v.28 no.4
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• pp.481-500
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• 2017

The purpose of this study is to analyze relationship between printed book and e-book formats based on niche theory. Readers with experience reading printed book and e-book were recruited as the research participants. In this study, competition between printed book and e-book formats was measured at gratification niche. five gratifications were derived from an analysis of precedence about printed book and e-book; information acquisition, social interaction & decision making, emotion & habit, portability & storage capacity, and accessibility. The results show that e-book formats have competitive superiority of portability & storage capacity and accessibility. This study suggests that it is possible to replace printed book and e-book formats.

• Journal of Korean Library and Information Science Society
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• v.49 no.1
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• pp.233-265
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• 2018

The aim of this study was to assess the social impact of the cultural programs of the children's libraries in Korea and further present the libraries'social value. To this end, we have conducted a survey on 511 elementary school students to assess the social impact of the children libraries'cultural programs. As a consequence, we have ascertained the children's expressiveness / creativity within their personal realms, self-esteem, pleasure / joy / happiness as well as their attitude towards cultural arts and changes in the cultural and artistic experiences further to growth in friendship as a matter of social interaction, generation and strengthening of local communities, sense of affiliation with local community / identify, and local community participation, among the manifestations of the social impact concerned. Based on this, children's libraries can be regarded as an important social capital of the community in the following aspects. Children's libraries enhance the quality of life and children's interaction skills. It also make them feel attached to the local community. Children's libraries maintain local communities, contribute to the creation and strengthening of local communities, and encourage participation in local activities.

• Journal of the Korean Society for information Management
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• v.33 no.2
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• pp.177-200
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• 2016

The purpose of this study was to analyze how the Library and Information Science (LIS) related research efforts in Korea contributed to the progress of the Human Computer Interaction (HCI) discipline to identify the potential collaboration research partners and topics. The following data was used for the network and frequency analysis: titles, keywords and affiliation information extracted from the Korean journals serving the LIS fields as well as the conference proceedings of the Korean HCI society. Information communication technology, computer engineering, and technology industry had been active in research collaborations but LIS field was comparatively not active. This study was able to determine that LIS researchers carried out important research but they had not much interests in the topics that required interdisciplinary collaboration. However, there were a number of papers, which were not authored by LIS discipline researchers, in the LIS related journals. In addition, there are a few ongoing interdisciplinary research projects which included LIS researchers. This reveals that it is possible for the LIS field could play an important role in the future if the researchers actively participate the collaborative projects.

• IMMUNE NETWORK
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• v.12 no.4
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• pp.155-164
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• 2012

It is well established that blocking the interaction of EGFR with growth factors leads to the arrest of tumor growth, resulting in tumor cell death. ER414 is a human monoclonal antibody (mAb) derived by guided selection of the mouse mAb A13. The ER414 exhibited a ~17-fold lower affinity and, as a result, lower efficacy of inhibition of the EGF-mediated tyrosine phosphorylation of EGFR when compared with mAb A13 and cetuximab. We performed a stepwise in vitro affinity maturation to improve the affinity of ER414. We obtained a 3D model of ER414 to identify the amino acids in the CDRs that needed to be mutated. Clones were selected from the phage library with randomized amino acids in the CDRs and substitution of amino acids in the HCDR3 and LCDR1 of ER414 led to improved affinity. A clone, H3-14, with a ~20-fold increased affinity, was selected from the HCDR3 randomized library. Then three clones, ER2, ER78 and ER79, were selected from the LCDR1 randomized library based on the H3-14 but did not show further increased affinities compared to that of H3-14. Of the three, ER2 was chosen for further characterization due to its better expression than others. We successfully performed affinity maturation of ER414 and obtained antibodies with a similar affinity as cetuximab. And antibody from an affinity maturation inhibits the EGF-mediated tyrosine phosphorylation of EGFR in a manner similar to cetuximab.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.86-86
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• 2003

Phytophthora capsici is a pathogen on several economically important crops including pepper. In pepper growing areas in Korea, Phytophthora blight caused by p. capsici has been considered as the most serious problem in pepper production. The Oomycete attacks the roots, stems, leaves and fruits of the plant. To understand the molecular mechanisms involved in the disease development, the genes expressed doting pepper p. capsici interaction were explored by analyzing expressed sequence tags (ESTs). A complementary DNA (cDNA) library was constructed from total RNA extracted from pepper leaves challenged with p. capsici for 3 days resulting in early stage of symptom development. The comprehensive analysis on the single pass sequencing of over 4000 randomly selected cDNA clones with contig assembly, unique gene extraction, sequence comparison, and functional categorizing will be presented with an emphasis on the genes involved in plant defense and pathogenicity during disease development of the pepper Phytophthora blight.

• Biomedical Science Letters
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• v.12 no.4
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• pp.405-411
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• 2006

Heterotrimeric GTP binding proteins (G proteins) mediate signal transduction generated by neurotransmitter and hormones. Among G-proteins, Go is classified as a member of the Go/Gi family and the most abundant heterotrimeric G protein in brain. Most of the mechanistic analyses on the activation of Go indicated its action to be mediated by the $G{\beta}{\gamma}$ dimer because downstream effectors for its ${\alpha}$ subunit have not been clearly defined. To determine the downstream effectors of alpha subunits of Go ($Go{\alpha}$), we used yeast two-hybrid system to screen $Go{\alpha}$ interacting partners in cDNA library from the human brain. A brain specific high mobility group box containing protein (BHX), A possible transcription factor, was identified as a $Go{\alpha}$ interacting protein. We confirmed interaction between $Go{\alpha}$ and BHX employing in vitro affinity binding assay. Moreover, active form of $Go{\alpha}$ preferentially interacts with BHX than inactive farm. Our findings indicate that $Go{\alpha}$ could modulate gene expression via interaction with BHX during neuronal or brain development.