• Title/Summary/Keyword: Lethal rate

Search Result 201, Processing Time 0.037 seconds

The Protective Effect of Melatonin Administration against Adria-mycin-induced Cardiotoxicity in Rats

  • Han, Jin;Kim, Chung-Hee;Kim, Na-Ri;Park, Ju-Hee;Yang, Young-Churl;Kim, Eui-Yong
    • The Korean Journal of Physiology and Pharmacology
    • /
    • v.5 no.4
    • /
    • pp.333-342
    • /
    • 2001
  • Adriamycin is a commonly used chemotherapeutic agent for cancer, including acute leukemia, lymphoma, and a number of solid human tumors. However, recent studies have recognized severe cardiotoxicity after an acute dose, which are likely the result of generation of free radicals and lipid peroxidation. Therefore, the clinical uses of adriamycin have been limited. Melatonin, the pineal gland hormone known for its ability to modulate circardian rhythm, has recently been studied in its several functions, including cancer growth inhibition, stimulating the immune system, and acting as an antioxidant and radical scavenging effects. In the present study, we evaluated the effect of melatonin administration on adriamycin-induced cardiotoxicity in rat. Heart slices were prepared using a Stadie-Riggs microtome for the measurement of malondialdehyde (MDA) content used as an index of lipid peroxidation and lactate dehydrogenase (LDH) release as an indicator of lethal cell injury. Serious adriamycin-induced lethality was observed in rat by a single intraperitoneal injection in a dose-dependent manner. A single injection of adriamycin (25 mg/kg, i.p.) induced a lethality rate of 86%, with melatonin (10 mg/kg s.c. for 6 days) treatment reducing the adriamycin-induced lethality rate to 20%. The severe body weight loss caused by adriamycin was also significantly attenuated by melatonin treatment. Treatment of melatonin marked reduced adriamycin-induced the levels of MDA formation and LDH release. A cell damage indicated by the loss of myofibrils, swelling of the mitochondria as well as cytoplasmic vacuolization was seen in adriamycin-treated group. Melatonin attenuated the adriamycin-induced structural alterations. These data provide evidence that melatonin prevents adriamycin-induced cardiotoxicity and might serve as a combination with adriamycin to limit free radical-mediated cardiotoxicity.

  • PDF

Squamous cell carcinoma of the maxillary sinus mimicking periodontitis

  • Na, Ji Yeon;Kang, Joo Hyun;Choi, Seong-Ho;Jeong, Ho-Gul;Han, Sang-Sun
    • The Journal of the Korean dental association
    • /
    • v.55 no.4
    • /
    • pp.276-283
    • /
    • 2017
  • Maxillary sinus squamous cell carcinoma is a relatively rare disease, comprising only 3% of malignant diseases of head and neck. As the growth rate is high and its prognosis is poor compared to others, the 5-year survival rate of maxillary sinus squamous cell carcinoma(MSSCC) is 23.4-49%. We introduce two rare clinical cases of squamous cell carcinoma originated from maxillary sinus of which symptoms include toothache and gingival swelling. On clinical examinations of both patients, deep periodontal pockets on upper right posterior teeth were detected. On panoramic images, the bony destruction of the maxillary sinus and its surrounding structures were not obvious and only alveolar bone loss was noted. It is difficult to diagnose MSSCC at an early stage due to symptoms of tooth pain and gingival swelling that are similar to that of periodontal diseases. However, if the symptoms do not improve after routine treatment of upper teeth, dentists should bear in mind of underlying malignant mass as differential diagnosis, thus early detection of the lethal disease. The aim of this study is to caution dental practitioners that malignancies have a potential to mimic periodontal diseases by introducing two cases of maxillary sinus squamous cell carcinoma presented as periodontitis.

  • PDF

The Experimental Study on Anti-thrombotic and Anti-inflammatory Effect of ChungyeolHaedogHwaeoTang(CHHT) (청열해독화어탕(淸熱解毒化瘀湯)의 항혈전(抗血栓) 및 항염(抗炎)효과에 관한 실험적(實驗的) 연구(硏究))

  • Kim, Sung-Ran;Ahn, Sang-Woo;Yoo, Dong-Yeoul
    • Korean Journal of Oriental Medicine
    • /
    • v.11 no.2
    • /
    • pp.97-111
    • /
    • 2005
  • This study was performed to evaluate anti-thrombotic activities and anti-inflammatory effects of ChungyeolHaedogHwaeoTang water extract(CHHT). The results were summarized as follows. In experiment of anti-thrombotic effect; 1. CHHT inhibited human platelet aggregation induced by ADP and epinephrine as compared with the control group, and inhibited pulmonary embolism induced by collagen and epinephrine (inhibitory rate is 37.5%). 2. CHHT increased platelet number significantly, and also CHHT shortened PT and APTT significantly as compared with the control group in thrombus model induced by dextran. In experiment of anti-inflammatory effect; 3. CHHT inhibited $IL-1{\beta}$, IL-6, TNF-${\alpha}$, COX-2 and NOS-II mRNA expression as compared with the control group in a concentration-dependent degree, and inhibited NO production significantly at 50, 100 ${\mu}g/ml$, and also inhibited ROS production in a concentration-dependent degree as compared with the control of group in RAW 264.7 cell line. 4. CHHT inhibited $IL-1{\beta}$, IL-6 and TNF-${\alpha}$ production significantly in serum of acute inflammation-induced mice, and decreased $IL-1{\beta}$, IL-6 and TNF-${\alpha}$ production in spleen tissue, and also decreased $IL-1{\beta}$, and IL-6 production in liver tissue, but increased TNF-${\alpha}$ production in liver tissue of acute inflammation-induced Balb/c mice. 5. CHHT increased survival rate from the 3rd day in ICR mice with lethal endotoxemia induced by LPS. These results suggest that CHHT can be useful in treating diverse female diseases caused by thrombosis and inflammation such as menstrual pain, menstrual disorder, leukorrhea, vaginitis, cervicitis, pelvic inflammatory disease and so on.

  • PDF

Selection of the Optimal Transplanting Method and Time for Restoration of Zostera marina Habitats (잘피(Zostera marina)서식지 복원을 위한 최적 이식방법 및 시기 선정에 관한 연구)

  • Park, Jung-Im;Kim, Young-Kyun;Park, Sang-Rul;Kim, Jong-Hyeob;Kim, Young-Sang;Kim, Jeong-Bae;Lee, Pil-Yong;Kang, Chang-Keun;Lee, Kun-Seop
    • ALGAE
    • /
    • v.20 no.4
    • /
    • pp.379-388
    • /
    • 2005
  • Seagrass bed is an important component in coastal and estuarine ecosystems, providing food and shelter to a wide variety of fauna. Recently, seagrass coverage has declined significantly due to anthropogenic influences such as reclamation, dredging, and eutrophication and consequently, necessity of seagrass habitat restoration is rising. Transplantation experiments with Zostera marina using TERFS, staple method, and shell method have been conducted at Dadae Bay, Kosung Bay and Jindong Bay on the south coast of Korea to select an optimal transplanting method for restoration of Z. marina habitat. Three experimental sites located at the vicinity of natural Z. marina beds with an average water depth of about 4m. Z. marina plants, which were collected from donor bed in Koje Bay were also transplanted at 7 different time from October 2003 to July 2004 to find appropriate transplanting time. Density of Z. marina was monitored monthly at both transplanted areas and natural beds. Transplantation using the staple method showed the highest survival rate of transplant. Shell method was also an effective transplanting method at muddy areas in Kosung Bay and Jindong Bay, but not suitable at sandy areas in Dadae Bay. These results suggest that sediment composition of transplanting areas should be considered for the selection of the optimal transplanting method. Z. marina transplanted during fall usually showed the highest survival rate, while most Z. marina plants transplanted in summer died due to high lethal temperature during this period.

Induction of Mutants by Irradiation of $\gamma$-Ray on In vitro Shoots of Persimmon (기내$\gamma$-선 처리에 의한 감(Diospyros kaki Thunb.) 돌연변이 유기)

  • 고갑천
    • Korean Journal of Plant Tissue Culture
    • /
    • v.27 no.2
    • /
    • pp.143-148
    • /
    • 2000
  • This study was carried out to know the optimum dose of gamma-ray for the induction of mutation in vitro and the characteristics of the mutants induced by gamma-ray in persimmon (Diospyros kaki Thunb.). The LD50 (50% lethal dose) for in vitro shoots of the cultivar, Nishimurawase was between 1 krad and 2 krad and about 1 krad for the cultivar, Ichikikeijiro. As the dose of gamma-ray increased, the length of shoots decreased and necrosis of buds increased. For the cultivar, Nishmurawase, 37.5∼58.3% shoots rooted and the rooting rate and the number of roots per shoot was low in high gamma-ray. The irradiated young plants which were grown in the growth cabinet for 6 weeks were shorter in shoot length and had more branches than non-irradiated plants. The survival rate of irradiated plants grown in the green house for 3 months was 33%, while 77% for control plants.

  • PDF

Effect of Temperature on the Development of the Ash-gray Leaf Bug, Piesma maculata (Insecta, Hemiptera, Piesmatidae) (온도가 두줄명아주노린재의 발육에 미치는 영향 (곤충망, 매미목, 명아주노린재과))

  • Park, Pil Ryoun;Sang Ock Park
    • The Korean Journal of Ecology
    • /
    • v.6 no.1
    • /
    • pp.55-65
    • /
    • 1983
  • The authors reared the ash-gray leaf bug, Piesma maculata in the growth cabinet controlled as temperature groups of $15, 20, 25, 30, 40^{\circ}C$under condition of photoperiod 16L:8D, light intensity $510{\pm}240$ lus, relative humidity $65{\pm}3%$, and analyzed the effects of temperature on the development of the insect. The results are summarized as follow: There are highly significant differences the developmental periods for the temperature groups, and between the developmental periods for the developmental stages. The egg in the temperature of 15 and $40^{\circ}C$ was hatched, but the ecdysis was impossible. The thermal threshold was $12.34^{\circ}C$and the upper lethal temperature $40.39^{\circ}C$. The total developmental periods of egg to adulate in the temperature of 20, 25, 30 and $35^{\circ}C$are 40.52, 22.37, 15.91 and 13.00 days, respectively. That is, the developmental period was decreased, as the temperature was increased. In the developmental period for the developmental stages, the developmental period of egg stage was longer than that of 25, 30 and $35^{\circ}C$, and that of 25。C was longer than that of $35^{\circ}C$. But ther was not significant differences between the developmental periods for the other temperature group. The rate of hatch at$20^{\circ}C$is the greater value as 90%, and the rates of 25, 30 and $35^{\circ}C$ are 79, 79 and 67%, respectively. That is the rate of hatch was decreased, as the temperature was increased. The mortality in the temperature of $35^{\circ}C$ is the greatest value as 68%, and those of 30, 25 and $20^{\circ}C$are 59, 59 and 41%, respectively. That is, the mortality was increased, as the temperature was increased. There was not significantly differences between the developmental period of female and male.

  • PDF

The Methanol Extract of Azadirachta indica A. Juss Leaf Protects Mice Against Lethal Endotoxemia and Sepsis

  • Kim, Woong-Hyun;Song, Hyun-Ok;Jin, Chun-Mei;Hur, Jong-Moon;Lee, Hwa-Sung;Jin, Han-Yong;Kim, Sung-Yeon;Park, Hyun
    • Biomolecules & Therapeutics
    • /
    • v.20 no.1
    • /
    • pp.96-103
    • /
    • 2012
  • In the present study, the inhibitory effect of neem leaf extract (NLE) on lipopolysaccaride (LPS)-induced nitric oxide (NO) and tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) production was examined both in vitro and in vivo. In vitro study revealed that NLE treatment ($100{\mu}g/ml$) inhibits LPS (100 ng/ml)-induced NO production by 96% and TNF-${\alpha}$ production by 32%. The reduction in NO production is probably conferred by the complete suppression of inducible nitric oxide synthase (iNOS) expression. Interestingly, in vivo NLE significantly improved the survival rate of mice in an experimental sepsis model. Administration of NLE (100 mg/kg) 24 h before LPS treatment (20 mg/kg) improved the survival rate of mice by 60%. The inhibition of plasma NO and TNF-${\alpha}$ production by NLE is likely to account for the improved survival of mice. Our results suggest that NLE may present a promising avenue in the development of therapeutic agents for the treatment of inflammatory diseases.

Effect of Popcorn, Wolbachia Variant, on Development and Reproduction in Drosophila melanogaster (Wolbachia 변이종 Popcorn이 초파리의 발육과 생식에 미치는 영향)

  • 한상미;민경태;한명세;이상철
    • Korean journal of applied entomology
    • /
    • v.39 no.3
    • /
    • pp.193-197
    • /
    • 2000
  • Wolbachia, vertically transmitted bacterial endosymbionts, is known to induce cytoplasmic incompatibility (CI), parthenogenesis, or feminization in some insect species. The Wolbachia found to exist symbiotically as a non-virulent form in Drosophila melanogaster, however popcorn is a lethal strain of Wolbachia, which causes tissue degeneration and early death of its adult host. Popcorn-infected flies showed the delayed duration of an egg and larvae, and their pupal period and life span reduced. The oviposition and egg-hatching rate of popcorn-infected flies were decreased 15% and 80%, respectiv~ly, compared to those of the normal Wolbachia-infected flies. The pupation and emergence rates of popcorn-infected flies were 67% and 65%, respectively. When popcorn-infected flies were crossed with Wolba-chia-infected flies, and vice versa, both crosses resulted in a significant reduction in egg production, egg¬hatching, pupation, and emergence rate, and their progeny revealed the popcorn syndrome. When popcorn-infected male flies were crossed with uninfected females, popcorn was not detected in their progeny. Popcorn also maternally transmitted in flies, but it did not induce CI and affected on its host life cycle as a virulence.

  • PDF

Proton Beam Sensitivity of Basidiospore and Mycelium in Pleurotus ostreatus (느타리버섯 포자와 균사체의 양성자 빔 조사에 대한 민감도)

  • Kwon, Hye-Jin;Kong, Won-Sik
    • The Korean Journal of Mycology
    • /
    • v.34 no.1
    • /
    • pp.34-38
    • /
    • 2006
  • To assess the effects of proton beam on radiation sensitivity of the basidiospore and mycelium of oyster mushroom (Pleurotus ostreatus), the $D_{10}$ values and $L_{50}$ (lethal 50%) values were analysed. By the proton beam radiation, the survival rate and germination rates increased at the dose of $10\;Gy{\sim}100\;Gy$ and then decreased significantly over 500 Gy. $L_{50}$ values of basidiospore and mycelium of Pleurotus ostreatus were over 500 Gy and 400 Gy, respectively. $D_{10}$ values were calculated from linear regression formulae ($D_{10}\;=\;-1/slope(b)$, y = a + bx) as 750 Gy and 1,250 Gy, respectively. Based on our experiment, the optimum dose of proton beam as a mutation source would be between from 500 Gy to 750 Gy for basidiospores and from 400 Gy to 1000 Gy for mycelium of oyster mushroom.

Experimental Study on Anti-thrombotic and Anti-inflammatory Effect of Kami-BoyangHwanoh-Tang (가미보양환오탕(加味補陽還五湯)의 항혈전(抗血栓) 및 항염작용(抗炎作用)에 대한 실험적 연구)

  • Lee, Jung-Eun;Yoo, Dong-Youl
    • Journal of Physiology & Pathology in Korean Medicine
    • /
    • v.20 no.4
    • /
    • pp.957-965
    • /
    • 2006
  • This study was peformed to evaluate antithrombotic activities and anti-inflammatory effects of Kami-BoyangHwanoh-Tang(KBHT). The major findings were summarized as follows. In experiment of anti-thrombotic effect; KBHT inhibited human platelet aggregation induced by ADP and epinephrine as compared with the control group and inhibited pulmonary embolism induced by collagen and epinephrine (inhibitory rate is 50 %). KBHT increased platelet number significantly and also KBHT shortened PT and APTT significantly as compared with the control group in thrombus model induced by dextran. In experiment of anti-inflammatory effect; KBHT inhibited $IL-1{\beta}$, IL-6, $TNF-{\alpha}$, COX-2 and NOS-II mRNA expression as compared with the control group in a concentration-dependent degree, and inhibited NO production significantly at 50, $100\;{\mu}g/^{ml}$, and also inhibited ROS production in a concentration-dependent degree as compared with the control group in RAW 264.7 cell line. KBHT inhibited $IL-1{\beta}$, IL-6 and $TNF-{\alpha}$ production significantly in serum of acute inflammation-induced mice, and decreased $IL-1{\beta}$, IL-6 and $TNF-{\alpha}$ production in spleen tissue, and also decreased IL-6 and $TNF-{\alpha}$ production in liver tissue, but increased $IL-1{\beta}$ production in liver tissue of acute inflammation-induced mice. KBHT increased survival rate at 3rd day in mice with lethal endotoxemia induced by LPS. These results suggest that KBHT can be useful in treating diverse female diseases caused by thrombosis and inflammation such as endometrosis, myoma, pelvic congestion, chronic cervicitis, chronic pelvic inflammatory disease and so on.