Browse > Article
http://dx.doi.org/10.4489/KJM.2006.34.1.034

Proton Beam Sensitivity of Basidiospore and Mycelium in Pleurotus ostreatus  

Kwon, Hye-Jin (Doorae Institute of Environment-Friendly Agriculture)
Kong, Won-Sik (Applied Microbiology Division,National Institute of Agricultural Science and Technology, R.D.A.)
Publication Information
The Korean Journal of Mycology / v.34, no.1, 2006 , pp. 34-38 More about this Journal
Abstract
To assess the effects of proton beam on radiation sensitivity of the basidiospore and mycelium of oyster mushroom (Pleurotus ostreatus), the $D_{10}$ values and $L_{50}$ (lethal 50%) values were analysed. By the proton beam radiation, the survival rate and germination rates increased at the dose of $10\;Gy{\sim}100\;Gy$ and then decreased significantly over 500 Gy. $L_{50}$ values of basidiospore and mycelium of Pleurotus ostreatus were over 500 Gy and 400 Gy, respectively. $D_{10}$ values were calculated from linear regression formulae ($D_{10}\;=\;-1/slope(b)$, y = a + bx) as 750 Gy and 1,250 Gy, respectively. Based on our experiment, the optimum dose of proton beam as a mutation source would be between from 500 Gy to 750 Gy for basidiospores and from 400 Gy to 1000 Gy for mycelium of oyster mushroom.
Keywords
Germination rate; Mutation; Oyster mushroom; Proton beam; Sensitivity;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Bano, K. and Rajarathnam, S. 1988. Pleurotus Mushrooms. Part II. Chemical composition, nutritional value, Post-harvest Physiology, preservation and role as human food. CRC Crit. Rev. Food Sci. Nutr. 27: 87
2 Bobek, P., Galbavy, S. and Ozdin, L. 1998. Effect of oyster mushroom (Pleurotus ostreatus) on pathological changes in diernthylhydrazine-induced rat colon cancer. Oncol. Rep. 5: 727-730
3 Byun, M.O., Chung, J. C., You, C. H., Cha, D. Y. and Lee, D. H. 1997. Transformation of Pleurotus sajor-caju by complementation of PABA requiring mutant. Kor. J. Mycol. 25: 233-237   과학기술학회마을
4 Joh, J. H., Kim, B. G., Kong, W. S., Yoo, Y. B., Chu, K. S., Kim, N. K., Park, H. R., Cho, B. G. and Lee, C. S. 2004. Isolation and characterization of dikaryotic mutants from Pleurotus ostreatus by UV irradiation. Mycobiology 32: 88-94   과학기술학회마을   DOI   ScienceOn
5 Kong, W. S., Kim, D. H., Kim, Y. H., Kim, K. S., You, C. H., Byun, M. O. and Kim, K. H. 1997. Genetic variability of Flammulina velutipes monosporous isolates Kor. J. Mycol. 25: 111-120
6 Peiji, G., Yinbo, Q., Xin, Z., Mingtian, Z. and Yonhheng, D. 1997. Screening microbial strain for improving the nutritional value of. wheat and com straws as animal feed. Enzyme Microb. Technol. 20: 581-584   DOI   ScienceOn
7 Shugemitsu, T. 2003. 방사선으로 만드는 보다 나은 생활을 목표로 해서, 방사선과 산업 99: 1-5
8 Wang, H., Gao, J. and Ng, T. B. 2000. A new lectin with highly potent antihepatoma and antisarcoma activities from {he oyster mushroom Pleurotus ostreatus. Biochem. Biophys. Res. Commun. 275: 810-816   DOI   ScienceOn
9 Yoo, Y. B. 1992. Interspecific hybridization between Pleurotus cornucopiae and Pleurotus florida following protoplast fusion. Kor. J. Mycol. 20: 118-125   과학기술학회마을
10 Yoo,Y. B. and Cha, D. Y. 1992. Gene transfer in edible fungi using protoplasts. Pp 157-192. In: Chang, S. T., Buswell, J. A. and Miles, P. G. Eds. Genetics and breeding of edible mushrooms. Gordon and Breach Science Publisher, New York
11 Lee, Y. and Chang, H. 1999. Radiation sensitivity of basid- iospore and mycelium in Pleurotus ostreatus. J. Kor. Nuclear Soc. 31: 287-293   과학기술학회마을
12 Jwanny, E. W., Rashad, M. M. and Abodu, H. M. 1995. Solidstate fermentation of agricultural wastes into food through Pleurotus cultivation. Appl. Biochem. Biotechnol. 50: 71-78   DOI
13 Atsushi, T. 2003. Ion beam 육종연구의 개발과 특징. 방사선과 산업 99: 6-16