The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Proton Beam Sensitivity of Basidiospore and Mycelium in Pleurotus ostreatus

느타리버섯 포자와 균사체의 양성자 빔 조사에 대한 민감도

  • Kwon, Hye-Jin (Doorae Institute of Environment-Friendly Agriculture) ;
  • Kong, Won-Sik (Applied Microbiology Division,National Institute of Agricultural Science and Technology, R.D.A.)
  • 권혜진 (두레친환경농업연구소) ;
  • 공원식 (농업과학기술원 응용미생물과)
  • Published : 2006.06.30
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Abstract

To assess the effects of proton beam on radiation sensitivity of the basidiospore and mycelium of oyster mushroom (Pleurotus ostreatus), the $D_{10}$ values and $L_{50}$ (lethal 50%) values were analysed. By the proton beam radiation, the survival rate and germination rates increased at the dose of $10\;Gy{\sim}100\;Gy$ and then decreased significantly over 500 Gy. $L_{50}$ values of basidiospore and mycelium of Pleurotus ostreatus were over 500 Gy and 400 Gy, respectively. $D_{10}$ values were calculated from linear regression formulae ($D_{10}\;=\;-1/slope(b)$, y = a + bx) as 750 Gy and 1,250 Gy, respectively. Based on our experiment, the optimum dose of proton beam as a mutation source would be between from 500 Gy to 750 Gy for basidiospores and from 400 Gy to 1000 Gy for mycelium of oyster mushroom.

양성자 빔 조사 후 조사선량에 따른 느타리버섯(Pleurotus ostreatus) 담자포자 발아율 및 균사체 생존율이 $10{\sim}100\;Gy$조사선량에서 시그모이드 곡선을 그리며 증가하였으며, 이후 500y 이상 조사시 유의성있게 감소하였다. 50% 치사율($L_{50}$)을 나타내는 조사선량은 담자포자가 500 Gy, 균사체가 400 Gy로 나타났으며, $D_{10}$값($D_{10}\;=\;-l/slope(b)$, y = a + bx) 은 각각 750 Gy와 1,250 Gy로 나타났다. 느타리버섯 돌연변이원으로서 양성자 빔 이용시 적정 조사선량은 담자포자의 경우 $500{\sim}750\;Gy$, 균사체의 경우 $400{\sim}1,000\;Gy$가 바람직하다고 판단되었다.

Keywords

References

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