• Journal of the Korean Society of Environmental Restoration Technology
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• v.8 no.6
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• pp.69-79
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• 2005

This study was carried out to select proper species for early stage replantation in highway cut-slope area. In highway cut-slope area, sample plots of 106 were selected, and their vegetations and environmental factors were investigated. 1. We found total 172 species in the 106 cutting area of highway. The species of high frequency of highway cut-slope were found in the order of Lespedeza bicolor, Artemisia princeps var. orientalis, Festuca arundinacea, Erigeron annuus, Lespedeza cuneata, Lactuca indica var. laciniata, Eragrostis curvula, Dactylis glomerata, Oenothera lamarckiana, Wistaria floribunda, Humulus japonica, Commelina communis, Miscanthus sinensis, Pueraria thunbergiana, Pinus densiflora, etc. 2. The average vegetation coverage was over 90% in the study sites and the average coverage was 91.4% in the total cut-slope area. The species of high coverage of highway cut-slope area were found in the order of Festuca arundinacea, Eragrostis curvula, Lespedeza bicolor, Wistaria floribunda, Lespedeza cuneata, Dactylis glomerata, Artemisia princeps var. orientalis, Humulus japonica, Pueraria thunbergiana, Robinia pseudoacacia, Poa pratensis, Medicago sativa, Festuca ovina, Pinus densiflora, Parthenocissua tricuspidata, etc. 3. The total coverage in the foreign plants of Festuca arundinacea, Eragrostis curvula, Dactylis glomerata, Poa pratensis, Medicago sativa, Coreopsis drummondii and native plants of Lespedeza bicolor, Wistaria floribunda, Lespedeza cuneata, Amorpha fruticosa, Indigofera pseudotinctoria, Lespedeza cyrtobotrya were 57.52%. That is, the ecological succession of native herbs and parachute shrubs have delayed because the afforested plants occupy 57.52%. In future, the coverage of foreign herbs have to reduce, and the coverage of the native herbs and parachute shrubs must be increased. 4. The native seed of Artemisia sp., Miscanthus sinensis, Smilax china, Pueraria thunbergiana, Rubus crataegifolius, Rubus parvifolius, Pinus densiflora, Rhus chinensis, Albizzia julibrissin, Rhododendron mucronulatum, Clematis apiifolia, Zanthoxylum schinifolium, Prunus sargentii could be added in the seedling of the temperate south zone highway with the used seeds. The native seed of Artemisia sp., Miscanthus sinensis, Rubus crataegifolius, Rhododendron mucronulatum, Weigela subsessilis, Stephanandra incisa, Rhus chinensis, Pinus densiflora, Salix koreensis, Cocculus trilobus, Populus alba, Spiraea prunifolia for. simpliciflora, Clematis apiifolia, Lindera obtusiloba, Quercus serrata, etc., could be added in the seedling of the temperate middle zone highway with the used seeds. 5. We have some recommendation. The native plants have to growth in the highway cut-slope area instead of foreign plants to have good environmental ecology. The role of the foreign plants should be the plant for the initial several years in the highway cut-slope area. And, the native plants should growth in the next season. 6. We should protect shrubs and trees in the highway slope area because shrubs and trees can be more helpful in stabilizing of the slope area than herbs.

• Microbiology and Biotechnology Letters
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• v.39 no.1
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• pp.63-69
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• 2011

In this study, the antibacterial activity and the antioxidative effects, inhibitory effects on tyrosinase of Lespedeza cuneata G. Don extracts were investigated. MIC value of ethyl acetate fraction from L. cuneata G. Don on P. ovale (0.125%) showed that the antibacterial activity of the ethyl acetate fraction was higher than methyl paraben. The aglycone fraction of L. cuneata G. Don (14.63 ${\mu}g$/mL) showed the most prominent the free radical (1,1-diphenyl-2-picryl-hydrazyl, DPPH) scavenging activity ($FSC_{50}$). Reactive oxygen species (ROS) scavenging activities ($OSC_{50}$) of L. cuneata G. Don fraction on $Fe^{3+}$-EDTA/$H_2O_2$ system were investigated using the luminol-dependent chemiluminescence assay. The aglycone fraction of L. cuneata G. Don (0.07 ${\mu}g$/mL) showed the most prominent ROS scavenging activity. The protective effects of extract/fractions of L. cuneata G. Don on the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. The L. cuneata G. Don extracts suppressed photohemolysis in a concentration dependent manner (1 ~ 50 ${\mu}g$/mL). The inhibitory effects ($IC_{50}$) of L. cuneata G. Don extracts on tyrosinase were determined with ethyl acetate fraction (104.83 ${\mu}g$/mL) and aglycone fraction (27.55 ${\mu}g$/mL) of L. cuneata G. Don extract. These results indicate that L. cuneata G. Don extract/fractions can function as high potential as bactericide against the pathogenic bacteria and antioxidant in biological systems, particularly skin exposed to UV radiation by scavenging $^1O_2$ and other ROS, and protect cellular membranes against ROS. Extract/fractions of L. cuneata G. Don could be applicable to new functional cosmetics for antiaging, antioxidant, and antibacterial activity.

• Applied Biological Chemistry
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• v.50 no.4
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• pp.344-347
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• 2007

The aerial parts of Lespedeza cuneata were collected, air-dried and extracted with 95% aqueous EtOH. Then it was successively partitioned with n-hexane, $CH_2Cl_2$, EtOAc and $H_2O$. Repeated Sephadex LH-20 column chromatography on the EtOAc- and $H_2O-soluble$ fractions gave four compounds. Their structures were elucidated as quercetin (1), kaempferol (2), desmodin (3) and homoadonivernith (4) on the basis of spectroscopic evidences such as $^{1}H-NMR$, $^{13}C-NMR$, 2D-NMR and MS spectrum. Desmodin (3) and homoadonivernith (4) have not been reported from this plant so far.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.13 no.2
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• pp.42-51
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• 2010

Herbs and shrubs are employed for environmental restoration purposes. Among common herbs and shrubs, few species with low germination rates were selected and studied for enhanced germination rates and decreased germination times via soaking treatment. Rhus chinensis, incubator grown samples treated with the bacterial solution for 72hrs followed by immediate seeding showed the highest germination rate of 26.7% and germination period of 5.7 days, 3 days decrease from the control. Treatment of distilled water (t=3.79, p<0.01), nutrient broth (t=4.44, p<0.00) and bacterial solution (t=4.42, p<0.00) showed highly significant difference. In the case of soil tests, treating in the nutrient broth for 72 hrs followed by immediate seeding yielded the the highest germination rate of 23.3% with 7.3 days to initial germination, a decrease of 14.7 days with respect to the control. All the samples followed by immediate seeding showed significant difference (t=2.13, p<0.05). Incubator grown samples of Lespedeza cyrtobotrya showed different results. The incubator samples suspended for 48 hrs in distilled water followed by immediate seeding and 1 day drying displayed the highest germination rate of 96.7%, surpassing that of the control by 33.4%. The incubator samples treated with the distilled water and nutrient broth showed enhanced germination. But only the samples treated with distilled water and nutrient broth for 48hrs showed the increased germination in soil tests. All the sample treated for 24 hrs followed by immediate seeding or dried for 1 day showed initial germination as early as 1 day in incubator. The initial germinations were shortened in the samples treated with distilled water and nutrient broth for 48hrs in soil tests. Lespedeza cuneata incubator sample treated with nutrient broth for 24 hrs and dried for 1 day exhibited the highest germination rate of 83.3%, a 31.1% improvement over the control. The incubator samples treated with distilled water for 48 hrs (t=4.20, p<0.01) showed effective increase of germination. The treatment of distilled water (t=2.96, p<0.05) and bacterial solution (t=2.24, p<0.05) showed significant difference. The germination rates in soil were less than those of incubator and the control. The incubator samples treated with distilled water and bacterial solution displayed 1 day germination period, shortened by 1.3 days compared to the control. For soil grown samples, the samples treated with distilled water showed delayed initial germination and those treated with nutrient broth for 48hrs and bacterial solution for 72hrs shortened initial germination.

• Korean journal of food and cookery science
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• v.28 no.3
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• pp.275-283
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• 2012

This study was conducted to monitor the quality characteristics of Lespedeza cuneata ethanolic extracts, by a response surface methodology. The independent variables were the extraction temperature ($35{\sim}95^{\circ}C}$), extraction time (2~10 hr), and ethanol concentration (10~90%). The coefficients of the determinations (R2) were 0.8562 (p<0.1), 0.9787 (p<0.01), and 0.8344 (p<0.1) in total polyphenol, total flavonoid, and electron donating ability, respectively. The electron donating ability and nitrite scavenging effect were improved with an increase of ethanol concentration, rather than the extraction temperature. ORAC (Oxygen Radical Absorbance Capacity) with extraction conditions was 1,636.47~2,696.99 ${\mu}moles$ TE/g, and was increased with the increase of extraction temperature and 40~60% of ethanol concentration. Estimated conditions for the maximized extraction including the yield, total polyphenol, electron donating ability, nitrite scavenging effect, and ORAC, were $60{\sim}68^{\circ}C$ in extraction temperature, 6~7 hr in extraction time, and 38~60% in ethanol concentration.

• Journal of the Korean Society of Food Science and Nutrition
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• v.41 no.11
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• pp.1508-1514
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• 2012

The purpose of this study was to evaluate the antioxidant contents and antioxidant activities of solvent fractions from methanolic extract of sericea lespedeza. To determine the antioxidant compounds in solvent fractions from methanolic extract, total polyphenolic, flavonoid, tannin, and proanthocyanidin contents were measured by spectrophotometric methods. Solvent fractions were evaluated for antioxidative capacity according to DPPH and ABTS radical scavenging activities. Total polyphenolic contents were 12.44, 3.61, 6.39, 27.11, 20.00, and 9.32 mg gallic acid equivalent (GAE)/g extract residue (ER), respectively. Total flavonoid contents were 2.94, 9.92, 7.77, 9.27, 5.11, and 2.66 mg catechin equivalent (CE)/g ER, respectively. Total tannin contents were 8.75, 10.04, 7.42, 17.32, 11.65, and 7.61 mg tannic acid equivalent (TAE)/g ER, respectively. Total proanthocyanidin contents were 346.09, 63.50, 103.76, 288.62, 231.99, and $358.48{\mu}g$ CE/g ER, respectively. DPPH radical scavenging activities of solvent fractions from methanolic extract of sericea lespedeza were 20.62, 5.16, 9.29, 20.80, 20.00 and 20.79 mg Trolox equivalent (TE)/g ER, and ABTS radical scavenging activities were 33.86, 9.24, 17.36, 33.76, 33.49, and 33.86 mg TE/g ER, respectively. SOD-like activities were 4.12, 0.61, 2.01, 9.89, 13.47, and 11.82 units/mL, and ${\alpha}$-glucosidase inhibition activities were 93.85 and 61.64% at concentrations of 50 and $25{\mu}g/mL$ in the water fraction, respectively. The results of this study show that notable antioxidant activities in sericea lespedeza have significant health benefits.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.10 no.3
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• pp.52-59
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• 2007

This study was carried out to select proper species for early stage replantation in cutting-slope area of the Baekdu Range roadside. In the cutting-slope area and upper forest area of the cutting-slope area, sample plots of 46 were selected and their vegetations and environmental factors were investigated. We found total 90 species in the 46 plots. We found Festuca arundinacea in 27 plots of the 46 plots. We also found Lespedeza bicolor in 26 plots, Eragrostis curvula in 22 plots, Artemisia princeps var. orientalis in 19 plots, Erigeron annuus and Pinus densiflora in 15 plots, Lactuca indica var. laciniata in 14 plots, Miscanthus sinensis in 13 plots, Oenothera odorata, Commelina communis, and Humulus japonicus in 11 plots, Lespedeza cuneata in 10 plots, Salix koreensis and Salix hulteni in 9 plots, Festuca rubra, Youngia denticulata, Aster scaber, and Festuca arundinacea in 8 plots, Chenopodium album var. centrorubrum in 7 plots, Patrinia villosa and Erigeron canadensis in 6 plots, and Setaria viridis, Digitaria sanguinalis, and Dactylis glomerata in 5 plots.The dominancy of Festuca arundinacea, Eragrostis curvula, Lespedeza bicolor, Miscanthus sinensis, Pinus densiflora, Festuca arundinacea, Festuca rubra, Artemisia princeps var. orientalis, Erigeron annuus, Lespedeza cuneata, Dactylis glomerata, Salix koreensis, Alnus hirsuta, Pueraria thunbergiana, Medicago sativa, Lactuca indica var. laciniata, and Digitaria sanguinalis were 13.41% 11.87%, 4.88%, 4.56%, 3.38%, 2.29%, 2.14%, 2.14%, 1.99%, 1.95%, 1.54%, 1.47%, 1.44%, 1.37%, 1.15%, 1.13%, 1.07% in the roadside cutting-slope, respectively. The dominancy of Rhododendron mucronulatum, Lindera obtusiloba, Rhus trichocarpa, Quercus mongolica, Lespedeza bicolor, Rhododendron schlippenbachii, and Quercus serrata 22.15%, 13.36%, 13.14%, 12.03%, 11.74%, 11.20%, and 11.14% in shrub layer of the upper forest area, respectively. The species of Rhododendron mucronulatum, Lindera obtusiloba, Rhus trichocarpa, Rhododendron schlippenbachii, Zanthoxylum schinifolium, Weigela subsessilis, Stephanandra incisa, Rhamnus davurica, Ligustrum obtusifolium, Lindera erythrocarpa, Rosa multiflora, Rubus coreanus, Symplocos chinensis for. pilosa, Smilax china, Prunus sargentii, and Staphylea bumalda may be more helpful in stabilizing of the cutting-slope area and making harmony with the surrounding forest area.

• Korean Journal of Food Science and Technology
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• v.47 no.4
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• pp.492-498
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• 2015

Testosterone deficiency syndrome (TDS) is normally observed in elderly men and is a clinical and biochemical syndrome, characterized by a decline in plasmic testosterone levels resulting in a significant decrease in quality of life. The aim of this study was to evaluate the effect of a mixed extract of fenugreek seeds and Lespedeza cuneata (YHM) on TDS. Rats were divided into three groups: the negative control, YHM-40 (40 mg/kg), and YHM-80 (80 mg/kg) groups. After 4 weeks of YMH administration, an increase was observed in the plasmic testosterone levels, vastus lateralis muscular strength, forced swimming time, total sperm counts, and motile sperm counts in YHM-40 and YHM-80 groups compared to the negative control group. Moreover, sex hormone binding globulin, the epididymal fat pad, total plasmic cholesterol, and triglyceride levels were significantly decreased in the YHM-fed groups. However, prostate specific antigen, aspartate aminotransferase, and alanine aminotransferase levels did not differ among the groups. These results suggest that YHM may enhance testosterone levels in elderly men and alleviate TDS without common side effects.

• Journal of Korean Society of Forest Science
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• v.111 no.2
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• pp.234-241
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• 2022

We performed this study to determine the foliar nutrient concentration and the nutrient stocks of restoration areas and adjacent Pinus densiflora S. et. Z. (red pine) stands in opencast kaolinite mines in Sancheong-gun, Gyeongsangnam-do, southern Korea. We chose six sites to determine foliage nutrient concentrations and the nutrient stocks of soils (0-10 cm depth). The dominant vegetation planted in restoration areas comprised Quercus acutissima Carruth., P. koraiensis S. et. Z., Festuca arundinacea Schreb., and Lespedeza cuneata G. Don. Invading vegetation in the restoration areas comprised Alnus incana (L.) Medik., Robinia pseudoacacia L., and Lespedeza spp., among others. The carbon and nitrogen stocks at 10 cm soil depth were significantly higher in the red pine stands than those in the restoration areas, whereas those of phosphorus, potassium, and magnesium were not significantly different between the two areas. However, calcium stocks were significantly higher in the restoration areas than in the red pine stands. Nitrogen concentration in foliage was higher in L. cuneata (20.28 mg N g^-1) than that in F. arundinacea (5.67 mg N g^-1), whereas potassium concentration was twice as high in F. arundinacea (18.8 mg K g^-1) as that in L. cuneata (9.07 mg K g^-1). Foliar nitrogen concentrations in invasive vegetation such as A. incana, R. pseudoacacia, and Lespedeza spp. were twice or four times higher than those of Q. acutissima and P. koraiensis. Our results indicate the development of suitable vegetation and soil amendment treatments to improve poor soil environmental conditions in restoration areas are necessary following opencast kaolinite mining.

• Korean Journal of Veterinary Research
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• v.63 no.3
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• pp.27.1-27.9
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• 2023

Lespedeza cuneata (LC) is a perennial plant used in herbal medicine to treat numerous diseases, including prostatic hyperplasia, diabetes, early atherosclerosis, and hematuria. Reference collections of bioactive compounds of LC are crucial for the determination of their pharmacological properties. However, little is known regarding its anti-oxidative and anti-inflammatory effects in alveolar macrophage (MH-S) cells. This study examined whether LC can inhibit reactive oxygen species and Coal fly ash (CFA) induced inflammation in MH-S cells. The anti-oxidative effects of LC were evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays, anti-inflammatory effects were examined using nitric oxide (NO) assay, and cytotoxicity was analyzed using methyl thiazolyl tetrazolium assay. The expression of inflammatory cytokine genes was assessed through a reverse-transcription polymerase chain reaction. Our results revealed that LC exhibited high radical scavenging activity and a dose-dependent (7.8-1,000 ㎍/mL) inhibition of oxidation as compared to ascorbic acid and Trolox. It also inhibited CFA-induced NO production in MH-S cells. Moreover, it suppressed the CFA exposure-mediated expression of pro-inflammatory mediators and cytokines, including inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin (IL)-1β, IL-6, and tumor necrosis factor-α. These results suggest that LC is a potent antioxidant and anti-inflammatory agent that can be useful as a nutraceutical product.