Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Antioxidant and anti-inflammatory activities of Lespedeza cuneata in Coal fly ash-induced murine alveolar macrophage cells

  • Abdul Wahab (Department of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University) ;
  • Hwayong Sim (Department of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University) ;
  • Kyubin Choi (Department of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University) ;
  • Yejin Kim (Department of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University) ;
  • Yookyeong Lee (Department of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University) ;
  • Byungwook Kang (Department of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University) ;
  • Yu Seong No (Department of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University) ;
  • Dongyeop Lee (Department of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University) ;
  • Inseo Lee (Department of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University) ;
  • Jaehyeon Lee (Department of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University) ;
  • Hwajun Cha (Department of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University) ;
  • Sung Dae Kim (Department of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University) ;
  • Evelyn Saba (Department of Veterinary Biomedical Sciences, Faculty of Veterinary and Animal Sciences, Pir Mehr Ali Shah Arid Agriculture University) ;
  • Man Hee Rhee (Department of Veterinary Medicine, College of Veterinary Medicine, Kyungpook National University)
  • Received : 2023.06.10
  • Accepted : 2023.08.31
  • Published : 2023.09.30
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Abstract

Lespedeza cuneata (LC) is a perennial plant used in herbal medicine to treat numerous diseases, including prostatic hyperplasia, diabetes, early atherosclerosis, and hematuria. Reference collections of bioactive compounds of LC are crucial for the determination of their pharmacological properties. However, little is known regarding its anti-oxidative and anti-inflammatory effects in alveolar macrophage (MH-S) cells. This study examined whether LC can inhibit reactive oxygen species and Coal fly ash (CFA) induced inflammation in MH-S cells. The anti-oxidative effects of LC were evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays, anti-inflammatory effects were examined using nitric oxide (NO) assay, and cytotoxicity was analyzed using methyl thiazolyl tetrazolium assay. The expression of inflammatory cytokine genes was assessed through a reverse-transcription polymerase chain reaction. Our results revealed that LC exhibited high radical scavenging activity and a dose-dependent (7.8-1,000 ㎍/mL) inhibition of oxidation as compared to ascorbic acid and Trolox. It also inhibited CFA-induced NO production in MH-S cells. Moreover, it suppressed the CFA exposure-mediated expression of pro-inflammatory mediators and cytokines, including inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), interleukin (IL)-1β, IL-6, and tumor necrosis factor-α. These results suggest that LC is a potent antioxidant and anti-inflammatory agent that can be useful as a nutraceutical product.

Keywords

Acknowledgement

This work was supported by a National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIT) (No. 2022R1A2C1012963).

References

  1. Fabricant DS, Farnsworth NR. The value of plants used in traditional medicine for drug discovery. Environ Health Perspect 2001;109(Suppl 1):69-75.  https://doi.org/10.1289/ehp.01109s169
  2. Shi Q, Li L, Huo C, Zhang M, Wang Y. Study on natural medicinal chemistry and new drug development. Zhong Cao Yao 2010;41:1583-1589. 
  3. Deng F, Chang J, Zhang JS. New flavonoids and other constituents from Lespedeza cuneata. J Asian Nat Prod Res 2007;9:655-658.  https://doi.org/10.1080/10286020600979894
  4. Numata A, Hokimoto K, Yamaguchi H. C-Glycosylflavones in Lespedeza cuneata. Chem Pharm Bull 1980;28:964-965.  https://doi.org/10.1248/cpb.28.964
  5. Park BK, Kim CW, Kwon JE, Negi M, Koo YT, Lee SH, Baek DH, Noh YH, Kang SC. Effects of Lespedeza Cuneata aqueous extract on testosterone-induced prostatic hyperplasia. Pharm Biol 2019;57:89-97.  https://doi.org/10.1080/13880209.2018.1564929
  6. Ha SJ, Lee J, Song KM, Kim YH, Lee NH, Kim YE, Jung SK. Ultrasonicated Lespedeza cuneata extract prevents TNF-α-induced early atherosclerosis in vitro and in vivo. Food Funct 2018;9:2090-2101.  https://doi.org/10.1039/C7FO01666B
  7. Cho EJ, Lee SG, Kim DO. The effect of Lespedeza cuneata extract for antioxidative and whitening effect. J Life Resour Sci Res 2009;28:34-38. 
  8. Sanjeewa KKA, Jayawardena TU, Kim SY, Lee HG, Je JG, Jee Y, Jeon YJ. Sargassum horneri (Turner) inhibit urban particulate matter-induced inflammation in MH-S lung macrophages via blocking TLRs mediated NF-κB and MAPK activation. J Ethnopharmacol 2020;249:112363. 
  9. Hwang L, Ko IG, Jin JJ, Kim SH, Kim CJ, Hwang JJ, Choi CW, Chang BS. Attenuation effect of polydeoxyribonucleotide on inflammatory cytokines and apoptotic factors induced by particulate matter (PM10) damage in human bronchial cells. J Biochem Mol Toxicol 2021;35:e22635. 
  10. Raudoniute J, Stasiulaitiene I, Kulvinskiene I, Bagdonas E, Garbaras A, Krugly E, Martuzevicius D, Bironaite D, Aldonyte R. Pro-inflammatory effects of extracted urban fine particulate matter on human bronchial epithelial cells BEAS-2B. Environ Sci Pollut Res Int 2018;25:32277-32291.  https://doi.org/10.1007/s11356-018-3167-8
  11. Mantovani A, Allavena P, Sica A, Balkwill F. Cancer-related inflammation. Nature 2008;454:436-444.  https://doi.org/10.1038/nature07205
  12. Hussain SP, Hofseth LJ, Harris CC. Radical causes of cancer. Nat Rev Cancer 2003;3:276-285.  https://doi.org/10.1038/nrc1046
  13. Yun HY, Dawson VL, Dawson TM. Neurobiology of nitric oxide. Crit Rev Neurobiol 1996;10:291-316.  https://doi.org/10.1615/CritRevNeurobiol.v10.i3-4.20
  14. Ignarro LJ. Nitric Oxide: Biology And Pathobiology. Academic Press, San Diego, 2000. 
  15. Mori M. Regulation of nitric oxide synthesis and apoptosis by arginase and arginine recycling. J Nutr 2007;137(6 Suppl 2):1616S-1620S.  https://doi.org/10.1093/jn/137.6.1616S
  16. Saba E, Lee YS, Yang WK, Lee YY, Kim M, Woo SM, Kim K, Kwon YS, Kim TH, Kwak D, Park YC, Shin HJ, Han CK, Oh JW, Lee YC, Kang HS, Rhee MH, Kim SH. Effects of a herbal formulation, KGC3P, and its individual component, nepetin, on coal fly dust-induced airway inflammation. Sci Rep 2020;10:14036. 
  17. Bradley JR. TNF-mediated inflammatory disease. J Pathol 2008;214:149-160.  https://doi.org/10.1002/path.2287
  18. Thomson AW, Lotze MT. The Cytokine Handbook, Two-Volume Set. 4th ed. Elsevier, Burlington, 2003. 
  19. Lee H, Jung JY, Hwangbo M, Ku SK, Kim YW, Jee SY. Anti-inflammatory effects of Lespedeza cuneata in vivo and in vitro. Korea J Herbol 2013;28:83-92.  https://doi.org/10.6116/kjh.2013.28.4.83
  20. Brand-Williams W, Cuvelier ME, Berset CL. Use of a free radical method to evaluate antioxidant activity. LWT Food Sci Technol 1995;28:25-30.  https://doi.org/10.1016/S0023-6438(95)80008-5
  21. Ullah HM, Lee YY, Yun BS, Kim SD, Rhee MH. Inhibitory effect of Phellinus baumii Extract on CFA-induced inflammation in MH-S cells through nuclear factor-κB and mitogen-activated protein kinase signaling pathways. Evid Based Complement Alternat Med 2021;2021:5535630. 
  22. Kim HR, Son JH, Kim BR, Kim ID, Shin DH. Paper presented at 24th Asian-Pacific Weed Science Society Conference October 22-25, 2013. Bandung (Indonesia), 2013. 
  23. Mo S, Kim EY, Choi SH, Lee S, Lee S, Ahn JC. Sericea lespedeza (Lespedeza cuneata) whole plant extract enhances rat muscle mass and sperm production by increasing the activity of NO-cGMP pathway and serum testosterone. Trop J Pharm Res 2021;20:121-128.  https://doi.org/10.4314/tjpr.v20i1.18
  24. Kim SM, Kang K, Jho EH, Jung YJ, Nho CW, Um BH, Pan CH. Hepatoprotective effect of flavonoid glycosides from Lespedeza cuneata against oxidative stress induced by tert-butyl hyperoxide. Phytother Res 2011;25:1011-1017.  https://doi.org/10.1002/ptr.3387
  25. Kim MS, Sharma BR, Rhyu DY. Beneficial effect of Lespedeza cuneata (G. Don) water extract on streptozotocin-induced type 1 diabetes and cytokine-induced beta-cell damage. Nat Prod Sci 2016;22:175-179.  https://doi.org/10.20307/nps.2016.22.3.175
  26. Lee KS, Park SN. Cytoprotective effects and mechanisms of quercetin, quercitrin and avicularin isolated from Lespedeza cuneata G. Don against ROS-induced cellular damage. J Ind Eng Chem 2019;71:160-166.  https://doi.org/10.1016/j.jiec.2018.11.018
  27. Cho E, Ju H, Jeong C, Eom S, Heo H, Kim D. Effect of phenolic extract of dry leaves of Lespedeza cuneata G. Don on antioxidant capacity and tyrosinase inhibition. Korean J Hortic Sci Technol 2011;29:358-365. 
  28. Lee HJ, Lim GN, Park MA, Park SN. Antibacterial and antioxidative activity of Lespedeza cuneata G. Don extracts. Microbiol Biotechnol Lett 2011;39:63-69. 
  29. Floegel A, Kim DO, Chung SJ, Koo SI, Chun OK. Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods. J Food Compos Anal 2011;24:1043-1048.  https://doi.org/10.1016/j.jfca.2011.01.008
  30. Kedare SB, Singh RP. Genesis and development of DPPH method of antioxidant assay. J Food Sci Technol 2011;48:412-422.  https://doi.org/10.1007/s13197-011-0251-1
  31. Miller NJ, Rice-Evans CA. Factors influencing the antioxidant activity determined by the ABTS.+ radical cation assay. Free Radic Res 1997;26:195-199.  https://doi.org/10.3109/10715769709097799
  32. Kim DO, Chun OK, Kim YJ, Moon HY, Lee CY. Quantification of polyphenolics and their antioxidant capacity in fresh plums. J Agric Food Chem 2003;51:6509-6515.  https://doi.org/10.1021/jf0343074
  33. Zeb A. Concept, mechanism, and applications of phenolic antioxidants in foods. J Food Biochem 2020;44:e13394. 
  34. Bruckdorfer R. The basics about nitric oxide. Mol Aspects Med 2005;26:3-31.  https://doi.org/10.1016/j.mam.2004.09.002
  35. Cavaillon JM. Pro- versus anti-inflammatory cytokines: myth or reality. Cell Mol Biol (Noisy-le-grand) 2001;47:695-702. 
  36. Ullah HM, Kwon TH, Park S, Kim SD, Rhee MH. Isoleucilactucin ameliorates coal fly ash-induced inflammation through the NF-κB and MAPK pathways in MH-S cells. Int J Mol Sci 2021;22:9506. 
  37. Bai Y, Liu S, Jiang P, Zhou L, Li J, Tang C, Verma C, Mu Y, Beuerman RW, Pervushin K. Structure-dependent charge density as a determinant of antimicrobial activity of peptide analogues of defensin. Biochemistry 2009;48:7229-7239. https://doi.org/10.1021/bi900670d