• Journal of Applied Biological Chemistry
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• v.58 no.1
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• pp.61-64
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• 2015

As an edible coating, carnauba shellac wax (CSW) and lemongrass oil (LO) solution was prepared to extend the shelf life of 'Hongro' apples. During storage, there were significant differences in the populations of total aerobic bacteria, yeast, and molds between the coated apples and the control samples. The weight loss and vitamin C loss of apples coated with the CSW-LO solution were lower than those of the control. These results suggest that CSW-LO coating can be an effective method for improving quality of 'Hongro' apples.

• Natural Product Sciences
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• v.23 no.4
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• pp.235-238
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• 2017

Colorado potato beetle is a most destructive insect pest of potato throughout the world. Although utilization of chemical insecticides is a main method for management of this pest, their negative side-effects such as threat to humans and the environmental pollution prompted researchers to search for natural alternatives. Recently plant essential oils with low or without side-effects against noun-targeted organisms and with high availability were considered as safe bio-pesticides. In the present study, toxicity of essential oil of Iranian lemongrass, Cymbopogon citratus Stapf, was evaluated against 3th instar larvae and adults of Colorado potato beetle by a leaf dipping method. Results displayed essential oil had notable toxicity against both larvae and adults after 24 and 48 h exposure times. Probit analysis revealed $LC_{50}$ values (lethal concentration to kill 50% of population) with 95% confidence limits were 10.32 (9.17 - 11.72) and 7.76 (6.80 - 8.74) ${\mu}l/ml$ for larvae and 6.27 (4.82 - 8.15) and 4.35 (3.24 - 5.62) ${\mu}l/ml$ for adults after 24 and 48 h, respectively. Based on regression analysis, a positive correlation between log concentration of essential oil and insect mortality was achieved. Results indicated C. citratus essential oil can be candidate as a natural alternative to the harmful chemical insecticides in the management of Colorado potato beetle.

• Parasites, Hosts and Diseases
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• v.59 no.5
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• pp.465-471
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• 2021

Culicoides biting midges (Diptera: Ceratopogonidae) are hematophagous arthropod vectors that transmit epizootic arthropod-borne viruses (arboviruses). Arboviruses are recognized as causes of pregnancy loss, encephalomyelitis, and congenital malformations in ruminants. Therefore, continuous monitoring and control of Culicoides, which causes significant damage to industrial animals are necessary. We performed attraction and repellent tests in Culicoides using various essential oils, cow dung, and carbon dioxide (CO₂). Culicoides tended to move more to cow dung (60.8%, P<0.0001) and CO₂ (63.8%, P<0.01). To the essential oils as repellents, 26.1% (P<0.0001), 18.7% (P<0.001), and 25.5% (P<0.01) of the Culicoides moved to the lavender, lemongrass, and eucalyptus chamber, respectively. The Culicoides that moved to the 3 essential oils chambers showed markedly low activity. Collectively, it was showed that Culicoides tended to be attractive to cow dung and CO₂, and repellent from the 3 essential oils.

• International Journal of Clinical Preventive Dentistry
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• v.14 no.4
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• pp.216-221
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• 2018

Objective: In this study, we tried to investigate the antimicrobial activity of natural herbaceous plant-derived essential oils against oral disease-causing bacterial strains and establish the scientific evidences to apply them to the dentifrice, mouthwash, and other uses. Methods: We used the disk diffusion method with 23 types of 100% natural essential oils to verify the antimicrobial effect against Streptococcus mutans, Porphyromonas gingivalis, and Lactobacillus rhamnosus. After adhering to the surface of the plate culture medium, $20{\mu}l$ of the essential oil was prepared, and the diameter of the transparent ring was measured after incubation for 24 hours. All the experiments were repeated three times. Results: Among 23 types of oils, 13 were effective against all three strains: myrrh, ginger, basil, carrot seed, tea tree, patchouli, ylang ylang, cypress, lemongrass, cinnamon, peppermint, lavender, and eucalryptus. Seventeen oils were effective against S. mutans, and myrrh, basil, and carrot seed showed high antimicrobial activity. Eighteen oils were effective against P. gingivalis, and tea tree, carrot seed, and cinnamons showed high antimicrobial activity. Sixteen oils were effective against L. rhamnosus, and carrot seed and peppermint cinnamon showed high antimicrobial activity. Conclusion: Consequently, 13 essential oils showed the antimicrobial activity against three bacterial strains, which indicates these essential oils might be used as the effective materials to suppress the growth of oral-disease inducing microorganisms. Moreover, essential oils that have been analyzed activities in this study will be mixed up within the constant range and analyzed their antimicrobial effects to examine the synergistic activity among them.

• Research in Plant Disease
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• v.12 no.1
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• pp.51-54
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• 2006

The effectiveness of four plant oils (garlic, ginger, cinnamon and lemongrass) against a range of plant pathogenic organisms was tested in vitro. Of the four oils, two oils (garlic and cinnamon) showed relatively good antimicrobial activities. Activity of garlic oil was shown against the plant pathogenic bacteria Agrobacterium tumefaciens, Ewinia carotovora, Ralstonia solanacearum, Xanthomonas campestris, and the fungi Phytophthora infestans, Fusarium oxysporum, Collectotricum sp., whereas Acidovorax avenae and Pythium sp. showed the resistance towards garlic oil. Results from the planta bioassays under greenhouse conditions indicated that garlic oil significantly reduced the cucumber powdery mildew (Sphaerotheca fusca) and tomato powdery mildew (Erysiphe cichoracearum) showing control value 70.0-74.6% and 71.2%, respectively.

• The Korean Journal of Food & Health Convergence
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• v.6 no.1
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• pp.9-15
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• 2020

Salinity with sodic condition disturbs germination, retards emergence, and slow down seedling development of Lemon Grass (Cymbopogon citratus).Lemongrass is a perennial grass plant widely distributed worldwide and most especially in tropical and subtropical countries. This research experiment was designed to evaluate the influences of (4 dSm^-1+ 13.5 (mmol L^-1)^-1/2, 5 dSm^-1+ 25 (mmol L^-1)^1/2, 5 dSm^-1+ 30 (mmol L^-1)^1/2, 10 dSm^-1+ 25 (mmol L^-1)^1/2 and 10 dSm^-1+ 30 (mmol L^-1)^1/2) on biomass produce of lemon grass against salt tolerance. The uppermost biomass yield (45.53 gpot^-1) was produced by 4 dSm^-1+ 13.5 (mmol L^-1)^1/2 treatment. The increase in the intensity of salts reduced the growth of lemon grass. Lower biomass yield (79.33 gpot^-1) was gained at 10 dSm^-1+ 30 (mmol L^-1)^1/2. 5 dSm^-1+ 25 (mmol L^-1)^1/2 treatment performed enhanced outcome i.e. the least reduction % over control (5.87). Salinity- sodicity showed serious effect on the growth reduction from 5.87% to33.60%. This reduction gap was affected by the negative effect of salinity and sodicity on Linseed growth. Salinity- sodicity showed severe impact on the growth reduction from 5.87% to33.60%. Based on the findings, lemon Grass (Cymbopogon citratus).was capable to grow up the maximum at 4 dSm^-1+ 13.5 (mmol L^-1)^1/2 treatment.

• Weed & Turfgrass Science
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• v.4 no.4
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• pp.321-329
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• 2015

The present study aimed to evaluate the phytotoxic potential of essential oils. For this purpose, 18 essential oil samples extracted from Korean plants and 64 commercial essential oils were screened for their phytotoxic potential against the seedling growth of Brassica napus L. (rapeseed). Among the 82 samples, 11 commercial oils (cinnamon, citronella, clove, cumin seed, geranium, jasmine, lemongrass, palmarosa, pimento, rose otto and spearmint) strongly inhibited the seedling growth with $GR_{50}$ value < $150{\mu}g\;mL^{-1}$. Major components from these effective essential oils were identified by solid phase microextraction/gas chromatography-mass spectrometry (SPME/GC-MS). GC-MS analyses revealed that the effective samples mainly consist of benzyl benzoate, carvone, citral, citronellol, eugenol, geraniol, D-limonene and terpinene. Subsequently, bioactivity of these individual components was evaluated against the seedling growth of B. napus, Echinochloa crus-galli and Aeschynomene indica. The components from different chemical groups exhibited different potency in inhibiting the seedling growth with varied $GR_{50}$ values ranged from $29{\mu}g\;mL^{-1}$ to > $1000{\mu}g\;mL^{-1}$. In the greenhouse experiment, citral and geraniol completely suppressed the growth of all the tested 10 plants at $100kg\;ha^{-1}$. In conclusion, the individual essential oil components geraniol and citral could be used as natural herbicides for weed management.

• Journal of Convergence for Information Technology
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• v.12 no.2
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• pp.192-197
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• 2022

Although various studies have focused on the relationship between essential oils (EOs) and skin flora, there are only few comparative studies on the antibacterial properties of EOs and their efficacy against common microorganisms. In this study, we tested the antibacterial activity of 10 different EOs against Staphylococcus epidermidis(S. epidermidis) with oxacillin as control. Optical density was used to measure the activity of solutions containing the EOs and S. epidermidis at two different concentrations. Three EOs (palmarosa, lemongrass, and Mellissa True) had higher antibacterial activity than oxacillin, but their concentrations had negligible effect on antibacterial activity.

• Journal of The Korean Society of Integrative Medicine
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• v.11 no.4
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• pp.73-82
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• 2023

Purpose : Cymbopogon citratus, also known as lemongrass, has widely spread around the world and its essential oil is usually applied in food, perfume, and other industrial purposes. In addition, C. citratus has also been used for the treatment of inflammation, digestive disorders, and diabetes in traditional medicine. In this study, the antioxidative activity of C. citratus ethanol extract (CCEE) was analyzed in RAW 264.7 cells through the induction of one of phase II enzymes, heme oxygenase (HO)-1 by nuclear factor-erythroid 2 p45-related factor (Nrf)2, mitogen-activated protein kinase (MAPK), and phosphoinositide 3-kinase (PI3K)/Akt. Methods : The antioxidative activity of CCEE against oxidative stress and its underlying molecular mechanisms were analyzed by the cell viability assay, intracellular reactive oxygen species (ROS) formation assay, and Western blot analysis in RAW 264.7 cells. Results : The results exhibited that CCEE potently attenuated tert-butyl hydroperoxide (t-BHP) induced intracellular ROS levels in a dose-dependent manner without any cytotoxicity. CCEE treatment significantly induced the expression of HO-1 which is known for its antioxidative capacity. In addition, CCEE treatment significantly upregulated the expression of Nrf2, a corresponding transcription factor for the regulation of antioxidative enzymes, which was in accordance with the HO-1 overexpression. MAPK and PI3K/Akt were also evaluated for their important roles in the regulation of cellular redox homeostasis against oxidative damage. As a result, the potent HO-1 expression was mediated by not extracellular regulated kinase (ERK), c-Jun NH2 terminal kinase (JNK), p38, but phosphoinositide 3-kinase (PI3K) phosphorylation. To confirm the antioxidative activity of CCEE-induced HO-1 expression, oxidative damage was initiated by t-BHP and attenuated by CCEE treatment, which was identified by HO-1 selective inhibitor and inducer. Conclusion : Consequently, CCEE potently induced the HO-1-mediated antioxidative potential through the modulation of Nrf2 and PI3K/Akt signaling pathways in RAW 264.7 cells. These results suggest that CCEE could be a promising strategy for the mitigation against cellular oxidative damage.