• Journal of Microbiology and Biotechnology
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• v.32 no.4
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• pp.464-472
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• 2022

An endo-polygalacturonase (endo-PGase) exhibiting excellent performance during acidic fruit juice production would be highly attractive to the fruit juice industry. However, candidate endo-PGases for this purpose have rarely been reported. In this study, we expressed a gene from Penicillium oxalicum in Pichia pastoris. The recombinant enzyme PoxaEnPG28C had an optimal enzyme activity at pH 4.5 and 45℃ and was stable at pH 3.0-6.5 and < 45℃. The enzyme had a specific activity of 4,377.65 ± 55.37 U/mg towards polygalacturonic acid, and the K_m and V_max values of PoxaEnPG28C were calculated as 1.64 g/l and 6127.45 U/mg, respectively. PoxaEnPG28C increased the light transmittance of orange, lemon, strawberry and hawthorn juice by 13.9 ± 0.3%, 29.4 ± 3.8%, 95.7 ± 10.2% and 79.8 ± 1.7%, respectively; it reduced the viscosity of the same juices by 25.7 ± 1.6%, 52.0 ± 4.5%, 48.2 ± 0.7% and 80.5 ± 2.3%, respectively, and it increased the yield of the juices by 24.5 ± 0.7%, 12.7 ± 2.2%, 48.5 ± 4.2% and 104.5 ± 6.4%, respectively. Thus, PoxaEnPG28C could be considered an excellent candidate enzyme for acidic fruit juice production. Remarkably, fruit juice production using hawthorn as an material was reported for the first time.

• Food Science of Animal Resources
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• v.43 no.2
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• pp.245-268
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• 2023

The effects of phosphate alternatives on meat quality in marinated chicken were investigated with the application of chilling and freezing. Breast muscles were injected with solution of the green weight containing 1.5% NaCl and 2% sodium tripolyphosphate (STPP) or phosphate alternatives. Treatment variables consisted of no phosphate [control (-)], 0.3% sodium tripolyphosphate [control (+)], 0.3% prune juice (PJ), 0.3% oyster shell, 0.3% nano-oyster shell, and 0.3% yeast and lemon extract (YLE) powder. One-third of the meat samples were stored at 4℃ for 1 d, and the rest of the meats were kept at -18℃ for 7 d. In chilled meat, a lower drip loss was noted for control (+) and YLE, whereas higher cooking yield in YLE compared to all tested groups. Compared with control (+), the other treatments except PJ showed higher pH, water holding capacity, moisture content, lower thawing and cooking loss, and shear force. Natural phosphate alternatives except for PJ, improved the CIE L^* compared to control (-), and upregulated total protein solubility. However, phosphate alternatives showed similar or higher oxidative stability and impedance measurement compared to control (+), and an extensive effect on myofibrillar fragmentation index. A limited effect was observed for C^*, h°, and free amino acids in treated meat. Eventually, the texture profile attributes in cooked of phosphate alternatives improved except for PJ. The results indicate the high potential use of natural additives could be promising and effective methods for replacing synthetic phosphate in chilled and frozen chicken with quality enhancement.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.33 no.4
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• pp.699-707
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• 2023

DevSecOps is a concept that adds security procedures to the operational procedures of DevOps to respond to the short development and operation cycle. Multi-step vulnerability scanning process should be considered to provide reliable security while supporting rapid development and deployment cycle in DevSecOps. Many open-source vulnerability scanning tools available can be used for each stage of scanning, but there are difficulties in evaluating the security level and identifying the importance of information in integrated operation due to the various functions supported by the tools and different security results. This paper proposes an integrated security metric design plan for scurity results and the combination of open-source scanning tools that can be used in security stage when building the open-source based DevSecOps system.

• The Journal of Economics, Marketing and Management
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• v.11 no.2
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• pp.23-32
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• 2023

Purpose: This study examines the effect of full information disclosure on seller profit when there exists information asymmetry between sellers and buyers, focusing on the risk averseness of buyers. By investigating the interaction between product quality and perceived risk through online sales data, we attempt to figure out the incentive structure of full information disclosure specifically when buyers are risk-averse, so that we can suggest more feasible information disclosure strategy to sellers. Research design, data and methodology: Our empirical model analyzes the sales data of collectible goods from a major online seller using Poisson regression. In our model, we have specifically considered risk-averseness of buyers by estimating the interaction effect between the product quality and perceived risk on seller profit, aiming for a more precise empirical analysis on sellers' incentive structure of full disclosure. Results: Our empirical analysis strongly supports the effect of interaction between product quality and perceived risk, showing that the incentive for full disclosure is much stronger when product quality is higher, and vice versa. Therefore, sellers are strongly encouraged to voluntarily reveal product weaknesses when their product quality is higher than average, while it is more profitable to hide any product defects when quality claim is lower than average. Conclusions: This study supports the related literature by confirming economic incentives for full disclosure, and also supplements and strengthens previous studies by presenting that the effect of interaction between product quality and perceived risk strongly affects seller profit. Our unique finding supports both mandatory disclosure and voluntary disclosure arguments and presents practical implications to marketing managers by suggesting that seller's incentive for revealing weaknesses depends on the level of seller's product quality.

• The Korean Journal of Mycology
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• v.51 no.3
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• pp.179-190
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• 2023

Grapevine downy mildew, caused by Plasmopara viticola, significantly damages vineyards and is one of the most devastating diseases affecting cultivated grapes worldwide. In this study, we characterized the phenotypic and molecular traits of 11 P. viticola isolates from four grape-growing regions in South Korea. Additionally, we investigated the diversity of pathogenicity among these isolates and conducted an assay to evaluate the response of grape cultivars to P. viticola infection. Lemon-shaped sporangia were identified in the collected isolates, which released zoospores into the suspension at room temperature. Within a few hours of inoculation, the zoospores developed germ tubes. We tested 11 P. viticola isolates for pathogenicity in 845 grape cultivars to screen for grape host resistance to downy mildew infection. Among the tested isolates, JN-9 showed the highest virulence. Grape cultivars displayed varying phenotypic reactions to P. viticola infection: approximately 7% were highly susceptible, 41% were susceptible, 20% were moderately susceptible, 8% were resistant, and 24% exhibited extreme resistance. Phylogenetic analysis based on four genomic regions (internal transcribed spacer 1 [ITS1], actin, beta-tubulin, and cytochrome c oxidase II) revealed a close evolutionary relationship among all the Korean isolates, forming a single monophyletic lineage. Notably, these isolates showed greater similarity to European isolates than to American isolates. This comprehensive study contributes to a deeper understanding of the identity and behavior of P. viticola, which is crucial for developing effective resistance strategies against this pathogen in grape cultivars cultivated in South Korea.

• Journal of Korean Society of Forest Science
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• v.14 no.1
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• pp.21-31
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• 1972

It has intended to identify the members of the Genus Lespedeza in Korea by a chemical colour reaction, and the following five species of the Genus Lespedeza grown in the garden have been used in this experiment. 1. Lespedeza bicolor Turcz 2. Lespedeza bicolor var. melanantha (Nak.) T. Lee 3. Lespedeza cyrtobotrya Miq. 4. Lespedeza japonica var. intermedia Nakai 5. Lespedeza maritima Nakai 6. Lespedeza maximowiczii Schneider 7. Lespedeza maximowiczii var. tomentella Nakai A few drops of each solution of $K_2Cr_2O_7$. $FeSO_4{\cdot}7H_2O$, $FeCl_3$, $KH_2PO_4$, $KMnO_4$, $NH_4OH$, and HCl was added to the methanol extracts of wood dust to get the specific colour reaction. HCl-infused wood was also used for the identification of L. bicolor var. melanantha and L. bicolor. The results can be summarized as the following key; 1. Chrome lemon by $K_2Cr_2O_7$ ${\cdots}{\cdots}$2 1. Sun flower yellow by $K_2Cr_2O_7$ ${\cdots}{\cdots}$Lespedeza maximowiczii var. tomentella Nakai 2. $KH_2PO_4$ Oystem white by $KH_2PO_4$; golden yellow by $FeCl_3$ ${\cdots}{\cdots}$=3 2. Cream colour by $KH_2PO_4$=6 3. Oyster white by $NH_4OH$; corn colour by $FeSO_4{\cdot}7H_2O$ ${\cdots}{\cdots}$4 3. Cream colcur by $NH_4OH$ ${\cdots}{\cdots}$5 4. Van dyke brown by $KMnO_4$ ${\cdots}{\cdots}$; sea shell pink by HCl injection under heating ${\cdots}{\cdots}$Lespedeza japonica var. intermedia Nakai 4. Sepia colour by $KMnO_4$; honey colour by HCl injection under heating ${\cdots}{\cdots}$Lespedeza maritima Nakai 5. Golden red by $FeSO_4{\cdot}7H_2O$; andover green by HCl-infused wood dust ${\cdots}{\cdots}$Lespedeza bicolor var. melanantha (Nak.) T. Lee 5. Yellow ochre by $FeSO_4{\cdot}7H_2O$; sand warm gray by HCl-infused wood dust ${\cdots}{\cdots}$Lespedeza bicolor Turcz 6. Amber green by $FeCl_3$ ${\cdots}{\cdots}$Lespedeza cyrtobotrya Miq. 6. Leather brown by $FeCl_3$ ${\cdots}{\cdots}$Lespedeza maximowiczii Schneider.

• Journal of Hospice and Palliative Care
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• v.15 no.2
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• pp.68-76
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• 2012

Purpose: The study was designed to verify effects of aromatherapy on depression, anxiety and the autonomic nervous system in breast cancer patients who are undergoing adjuvant radiotherapy. Methods: Data were collected from November 2006 through March 2007 at the C university hospital in Seoul. The study included 33 patients and they were assigned to three groups. Group I had aroma oil inhalation for 2 minutes per time, three times a day for six weeks whereas Group II and a control group inhaled aroma oil for 2 minutes per time, once a day for six weeks. For Groups I and II, a mixture of lemon, lavender, rosewood and rose essential oils were used while control group inhaled tea tree oil. Results: Depression was significantly decreased in patients in the experimental groups only. In Groups I and II patients, anxiety level was lower than that in control patients. Patients in Groups I and II also showed stronger physical resistance to stress than control group patients. Conclusion: Aromatherapy should be considered as a method that can significantly decrease depression in breast cancer patients who are undergoing adjuvant radiotherapy.

• Journal of Oral Medicine and Pain
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• v.30 no.2
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• pp.141-148
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• 2005

Since many reports for the influence of the essential oils on the oral microorganism were presented, it is important to use the essential oils in clinical field. At the present day which emphasized the quality of life, oral malodor is one of the serious problems, so we need to try to decrease of oral malodor. This study was designed to make on evidence of the effect of essential oils to oral malodor clinically and to develop on effective treatment for oral malodor by using the dental paste which contains essential oils. 40 dental students volunteered to participate in this study and double blind test was used. At the beginning, all subjects were measured their morning malodor prior to this experiment, at the 3cm posterior to their incisal edge of the oral cavity by Halimeter(Interscan Co. Chatsworth, CA). After that we divided the students in half into two groups A and B. We have supplied different tooth pastes, dental paste A and B, to two groups respectively. The dental paste B contained essential oils which are tea tree, lemon and peppermint. All students used different tooth pastes everyday for 3 weeks. And all students were measured their morning malodor every week with the same method by using Halimeter. This study was resulted that ; 1. The dental paste which contains essential oils has continuously decreased the morning malodor during the whole period of experiments. 2. The number of students with increase in their morning malodor was generally decreased in the group B who used the toothpaste which contains essential oils, compared with the group A who used the toothpaste which didn't contain essential oils on the 2nd and 3rd week of the experiment. 3. In the group B, the average data on increasing rate of morning malodor was less than in the group A. 4. In the 2nd and 3rd week of the experiment, the data on the rate of morning malodor of the group B was generally decreased continuously compared with the group A. These results have statistically significance under 95% confidence interval of the difference.(p$\leq$0.05) As the result, the toothpaste which was containing essential oils was effective in decreasing the morning malodor.

• Korean Chemical Engineering Research
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• v.54 no.1
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• pp.81-88
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• 2016

Limonene is orange flavored natural material that is mainly contained in mandarine and lemon peels. D-limonene was extracted from cold-storaged mandarine peel by using Soxhlet extractor at $120^{\circ}C$ for 2 hours with ethanol as solvent. Henry constants of d-limonene and impurity were calculated as $H_{Lim}=8.55$ and $H_{imp}=0.223$ from the result of HPLC analysis. 4-bed SMB of limonene simulation with $0.46{\times}25cm$ columns was conducted by using Aspen chromatography program. Then effective condition for purity was found by changing $m_2$ and $m_3$ values in triangle diagram. The highest purity was 98.59% at $m_2=2.57$, $m_3=9.55$. For this case, feed, desorbent, extract, and raffinate flow rates were 1 mL/min, 1.19 mL/min, 0.857 mL/min and 1.34 mL/min, respectively. Scale-up simulation was also conducted by increasing column diameter from 0.46 cm to 1.6 cm for getting the same efficiency. The increased flow rates were 12 mL/min, 14 mL/min, 10 mL/min, and 16 mL/min for feed, desorbent, extract, and raffinate. It was possible to scale-up with maintaining same limonene purity because linear isotherms of limonene and impurity were assumed.

• The Korean Journal of Pesticide Science
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• v.17 no.4
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• pp.419-427
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• 2013

The insecticidal activity of 124 plant essential oils and control efficacy of six experimental spray formulations (SF) containing 0.25, 0.5, 1, 2.5, 5, and 10% of the selected oils was examined against both nymph and adult of the citrus flatid planthopper, Metcalfa pruinosa using direct contact applications (leaf dipping and spray). Reponses varied according to dose (1,000 and 500 mg/L). When exposed at 1,000 mg/L for 24 h using leaf dipping assay, 19 essential oils showed strong mortality (100%) among 124 essential oils screened. At 500 mg/L, 100% mortality was observed in cinnamon technical, cinnamon green leaf, cinnamon #500, cassia tree, citronella java and pennyroyal followed by origanum, thyme white, grapefruit, savory, fennel sweet, aniseed and cinnamon bark showed considerable mortality (93.3-80%) against nymphs of M. pruinosa. The moderate mortality (73.3-60%) was found in thyme red, tagetes, calamus, lemoneucalptus and geranium. Oils applied as SF-10% sprays provided 100 % mortality against adult M. pruinosa. One hundred mortalities were achieved in cinnamon technical at >SF-0.5 formulation, in cinnamon #500, cinnamon green leaf and penny royal at >SF-2.5. To reduce the level of highly toxic synthetic insecticides in the agricultural environment, the active essential oils as potential larvicides could be provided as an alternative to control M. pruinosa populations.