• Applied Biological Chemistry
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• 제41권4호
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• pp.246-250
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• 1998

The cytotoxic activity of methanol extracts of 25 leguminous seeds in vitro was evaluated by sulforhodamine B assay, using the five human solid A549 lung, SK-OV-2 ovarian, SK-MEL-2 melanoma, XF-498 CNS and HCT-15 colon tumor cell lines. The responses varied with both cell line arid leguminous seed used. Extracts of Canavalia lineata and Glycine soja revealed potent cytotoxic activity against A549 arid SK-MEL-2 cell lines. Moderate activity was observed in the extracts of Cassia obtusifolia and Glyeine max var. chungtae, and C. lineata and Vigna angulasis against SK-MEL-2 and HCT-15 cell lines, respectively. The other seed extracts were ineffective against model tumor cell lines. Because of their potent cytotoxic activities, the activity of each solvent fraction from C. lineata and G. soja was determined and the potent activity was produced from their chloroform fractions. As a naturally occurring therapeutic agent, leguminous seeds described could be useful for developing new types of anti-tumor agents.

• Journal of Plant Biotechnology
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• 제39권3호
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• pp.121-126
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• 2012

본 연구에서는 국내에서 재배중인 대표적인 두과작물(대두, 완두, 강낭콩, 팥, 녹두, 동부)종자로부터 전세포추출물의 FT-IR 스펙트럼 데이터로부터 다변량통계분석(PCA, PLS-DA, HCA)을 이용하여 신속하고 간편한 종 구분체계를 확립하였다. 대사체수준에서 팥, 녹두, 동부는 유연관계가 높음을 알 수 있었으며 대두, 완두, 강낭콩은 비록 두과작물이지만 차이가 매우 큼을 알 수 있었다. 아울러 본 연구에서 얻어진 대사체 정보의 다변량통계분석에 의한 유연관계분석은 흥미롭게도 두과작물의 계통분류학적 유연관계와 밀접한 상관관계를 나타내었다. 따라서 FT-IR 스펙트럼 데이터의 다변량통계분석은 방법의 간편성과 신속성을 고려할 때 두과작물의 계통이나 품종의 신속한 식별 수단으로 활용이 가능할 것으로 기대된다. 또한 두과작물의 기능성 성분 함량 정보가 성공적으로 연계된다면 본 연구에서 확립된 대사체 기반 신속식별체계는 기능성 성분의 함량이 높은 계통이나 품종의 조기 선발수단으로 활용이 가능할 것으로 기대된다.

• 농약과학회지
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• 제2권3호
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• pp.21-27
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• 1998

25종 콩과식물 종자의 메탄올 추출물을 대상으로 기주식물상의 온실실험조건에서 6종의 주요 식물병원균에 대해 방제효과를 조사한 그 효과는 콩과식물의 종류에 따라 커다란 차이를 보였다. 25종의 공시종자를 5 mg/pot 농도로 처리하였을 때 결명자 (C. obtusifolia), 소리테(G. max var. solitae), 약콩(G. max var. yagkong), 흑태(G. max var. hooktae), (P. multiflorusf), (P. radiatus var. aurea)과 (V. sinensis) 추출물들은 오이잿빛곰팡이병, 밀붉은녹병 및 보리횐가루병에 대하여 80% 이상의 방제효과를 나타내었을 뿐 아니라, 잿빛곰팡이병의 살균제 저항성 균주에 대해서도 높은 활성을 보였다. 그러나, 공시종자 모두 벼도열병, 벼잎집무늬마름병 및 Phytophthora infestans병에 대해서는 거의 활성이 없거나 활성이 낮았다. 이상의 결과로부터 잿빛곰팡이병, 밀녹병 및 보리흰가루병 등에 높은 방제효과를 보인 상기 종자추출물들은 식물병 방제제로서 사용 가능성이 예상되었으며, 특히 살균제 저항성 잿곰팡이병에 높은 활성을 보여 시설원예재배에서 문제시되고 있는 잿빛곰팡이병의 방제에도 크게 이용할 수 있을 것으로 기대되었다.

• Journal of Applied Biological Chemistry
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• 제44권1호
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• pp.1-5
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• 2001

The methanol extracts of 25 leguminous seeds in vitro was evaluated for inhibitory activities against the small intestinal $\alpha$-glucosidase of Sprague Dawley male rats. The responses varied both with leguminous seed types and concentrations used. At the concentration of 0.5 mg/ml, the methanol extracts of Cassia obtusifolia, Glycine max var. yagkong, Glycine max var. hooktae, Glycine max var. geumdu, Glycine max var. mejukong, Glycine soja, Phaseolus multiflorus, Pisum sativum, and Vigna sinensis inhibited over 50% of the enzyme activity. The extracts of G. max var. yagkong and V. sinensis showed relatively strong inhibitory activities against $\alpha$-glucosidase at the concentration of 0.1 mg/ml. The activity of each solvent fraction from G. max var. yagkong and V. sinensis was determined, and potent activities were detected from chloroform and butanol fractions, respectively. $IC_{50}$ values of G. max var. yagkong and V. sinensis were 0.06 and 0.19 mg/ml, respectively. As a naturally occurring therapeutic agents, leguminous seeds examined could be useful for developing new types of antidiabetic agents.

• 한국응용곤충학회지
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• 제43권2호
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• pp.123-127
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• 2004

두류품종의 먹이조합에 따른 톱다리개미허리노린재의 약충발육, 성충수명 및 산란에 미치는 효과를 조사하였다. 약충기간은 20-28일로 장유콩(새알콩), 장유콩＋강낭콩 및 장유콩＋땅콩의 조합에서 가장 많았으며, 강낭콩에서 가장 길었다. 영기별 발육기간은 1령에서 2.4-2.5일, 2령에서 4.0-5.1일, 3령에서 4.2-6.0일, 4령에서 4.4-6.9일 그리고 5령에서 5.3-7.3일로 영기가 증가할수록 길어졌으며, 1령을 제외한 타령기에서 발육기간은 먹이조합에 따라 유의한 차이가 있었다. 우화율은 3.5-65%로 먹이조합에 따라 현저한 차이가 있었는데, 장유콩＋땅콩에서 65%로 가장 높았으며, 강낭콩에서 3.5%로 가장 낮았다. 약충사망율은 먹이조합에 관계없이 1령에서 가장 높았다. 성충수명은 5.5-11.2일로 장유콩＋땅콩에서 가장 길었으며, 강낭콩＋땅콩에서 가장 짧았고, 장유콩, 강낭콩, 땅콩 및 장유콩＋강낭콩에서 8.0-8.4일로 먹이에 따른 유의한 차이가 없었다. 총 산란수는 1.0-20.0개로 전체적으로 산란수가 매우 낮았으나, 장유콩＋땅콩＞장유콩＞딴콩＞장유콩＋강낭콩＞강방콩＞강낭콩＋땅콩의 순으로 많았다. 따라서 두류품종의 종자를 이용한 톱다리개미허리노린재의 사육은 장유콩＋땅콩 조합에서 가장 좋았으며, 다음은 장유콩이였다.

• Preventive Nutrition and Food Science
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• 제6권1호
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• pp.38-42
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• 2001

The methanol extracts of 25 leguminous seeds in vitro were evaluated for inhibitory activities against lens aldose reductase of Sprague Dawley male rats. The responses varied with both leguminous seed and concentration used. At the concentration of 0.1 mg/mL, the methanol extracts from Amphicaraea edgeworthii, Canavalia lineata, Gylcine max var. solitae, Glycine max var. yagkong, Glycine max var. hooktae, Glycine max var. bangkong, Glycine max var. geumdu, Glycine max var. chungtae, Glycine max var. mejukong, Glycine soja, Phaseolus radiatus var. geodu, Vicia tetrasperma, Vigna angulasis, and Vigna sinensis inhibited enzyme activity by greatertha 60%. In following study, at the concentration of 0.01 mg/mL, the extracts of C. lineata and V. tetraspermahad relatively strong inhibitory activity against aldose reductase. Because of their potent inhibitory activities, the activity of each solvent fraction from C. lineata and V. tetrasperma was determined, and the potent activity was showed from chloroform and hexane fractions, respectively. {TEX}$IC_{50}${/TEX} values of C. lineata and V. tetrasperma were 0.004 and 0.006 mg/mL, respectively. As a naturally occurring therapeutic agent, leguminous seeds described could be useful for developing new agents of antidiabetic complications.

• 한국작물학회지
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• 제50권1호
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• pp.32-38
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• 2005

A long-term study initiated in 1989 at San-born Field, Columbia, Missouri, was designed to evaluate the affect of environmental factors, nitrogen application, and crop rotation on soybean (Glycine max [L.] Merr.) seed composition. Soybeans were grown as part of a four- year rotation which included corn (Zea maize L.), wheat (Triticum aestivum L.), and red clover (Trifolium pratense L.). Results from soil tests made prior to initiation of the study and subsequently every five years, were used to calculate application rates of nitrogen, phosphorus, and potassium necessary for target yield of pursuant crops. In the experimental design, nitrogen was applied to one-half of the plot on which the non-leguminous crop, either corn or wheat was grown. Analysis of soybean seed by near infrared reflectance spectroscopy collected over an 11-year period revealed a linear increase in protein and decrease in oil content. Application of nitrogen fertilizer to non-leguminous crops did not have an apparent effect on total protein or oil content of subsequent soybean crop. Analysis of soybean seed proteins by sodium dodecyl sulfate polyacrylamide gel electrophoresis in conjunction with computer­assisted densitometry revealed subtle changes in the accumulation of seed proteins. Immunoblot analysis using antibodies raised against the $\beta-subunit$ of $\beta-conglycinin$ showed a gradual increase in the accumulation of the 7S components during successive years of the experiment. A linear increase in temperature and decrease in rainfall was observed from the onset of data· collection. Higher temperatures during the growing season have been linked to increased protein and diminished oil content of soybean, thus changes observed in this study are possibly related to climatic conditions. However, crop rotation and subsequent changes in soil ecology may contribute to these observed changes in the seed composition.

• 농업과학연구
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• 제39권4호
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• pp.483-489
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• 2012

Preference of stink bugs to various leguminous seeds was evaluated by using VTS (Video Tracking System) in laboratory. Major soybean stink bugs such as bean bug, Riptortus pedestris (Fabricius), one-banded stink bug, Piezodorus hybneri (Gmelin), eastern green stink bug, Nezara antennata (Scott), and sole bug, Dolycoris baccarum (L.) were significantly most attracted to Cheongjakong, a soybean variety, baited fish-net trap, followed by soybean varieties Ilpumgeomjeongkong and Taekwangkong, a peanut variety Daekwangdangkong, a kidney bean variety Gangnangkong, and a adzuki bean variety Jungwonpat, respectively in a soybean field. VTS observation in laboratory showed that R. pedestris and D. baccarum had significantly higher frequency of visit on Cheongjakong, followed by Ilpumgeomjeongkong. However, P. hybneri, N. antennata and Halyomorpha halys (Stal) had significantly higher number of visits on Cheongjakong, Seonnogkong and Jinpumkong, followed by Ilpumgeomjeongkong. The sojourned time of stink bugs, however, was significantly longer on Cheongjakong regardless of species of the bugs. Accordingly, Cheongjakong was evaluated as the most preferred soybean seed by fish-net trap and VTS. Thus, VTS is found to be an effective means to evaluate the food preference of stink bugs.

• 한국유기농업학회지
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• 제19권spc호
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• pp.247-250
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• 2011

Four seed vetch (Vicia tetrasperma), a biennial native leguminous plant, was used for a cover crop with different quantity of sowed seeds. Weed suppression and yield were evaluated for the red pepper cultivation in the following year of the vetch seeding. Seeding of 1.0, 2.0, 3.0, 4.5, and 6.0 kg 10 $a^{-1}$ suppressed weed occurrences until late in the growing season of the red pepper. Consequently, red pepper in the cover cropping system with seeding of 3.0, 4.5, and 6.0 kg 10 $a^{-1}$ had a similar yield to the conventional red pepper. The ideal seed rate in four seed vetch was 3.0 and 4.5 kg 10 $a^{-1}$ in terms of reducing weed occurrence as well as increasing growth and yield in red pepper.

• 환경생물
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• 제34권4호
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• pp.346-352
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• 2016

팥바구미(Callosobruchus chinensis L.)의 산란 선호성 및 성장 조사를 위해 6가지 콩과식물 종자인 팥, 흑태, 백태, 서리태, 서목태, 강낭콩을 선택, 실험을 하였다. 각 종자당 산란수는 팥 1.23개, 서목태 1.13개, 서리태 1.05개, 백태 0.69개, 흑태 0.61개로 나타났으며, 둘째 날(48 h) 최고 산란수, 넷째날(96 h) 최저 산란을 나타냈다. 유충 성장기간이 기주식물에 따라 2배 이상 증가하는 것으로 나타났다. 우화율은 팥, 서리태, 흑태, 백태, 서목태(83.33%, 28.23%, 27.87%, 20.44%, 11.59%) 순으로 나타났으며, 모든 종자에서 팥과 3배 이상의 차이를 보였다. 성충 수명은 암컷보다 수컷이 대부분 길었고 암컷의 경우 팥 9.43일, 서리태 8.86일, 흑태 7.88일, 백태 7.17일, 서목태 6.80일로 나타났다. 각 성충의 무게는 암수 모두 팥에서 가장 무겁고, 수컷은 서목태, 암컷은 백태에서 최소 무게를 나타냈다. 결과적으로, 팥바구미는 팥 이외의 기주에서는 산란, 우화율, 성충 수명, 성충 무게 감소가 나타났고 반면 우화시기도 지연되었다. 한편 강낭콩에서 산란과 우화는 전혀 없었는데 이것은 강낭콩 종피에 산란과 우화 억제물질이 있음을 시사하며, 이러한 물질의 활용으로 콩바구미과 피해를 조절할 수 있을 것으로 여겨진다.