• Journal of Embryo Transfer
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• v.30 no.3
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• pp.183-188
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• 2015

This study analyzed the coat color-related genes of MC1R, ASIP, ECA3-inversion, and STX17 of 1,462 Jeju horses administered by the Jeju Special Self-Governing Province. This was done to investigate the distributional characteristics of coat color-related genes in the Jeju horse group and the changes of its coat color-related genes by generation. The genotype frequency of the MC1R gene of $E^+/E^+$ and $E^+/E^e$ related to black coat color was 0.122 and 0.447, respectively, while $E^e/E^e$ of the chestnut genotype was 0.429. The genotype frequency of the ASIP gene of $A^A/A^A$, $A^A/A^a$, and $A^a/A^a$ was 0.46, 0.448, and 0.091, respectively, where the genotype frequency of $A^a/A^a$ turned out to be relatively low. The To/To and +/To genotype that manifests the Tobiano shape was 0.001 and 0.119, respectively, with the share of Tobiano shape around 12%. The genotype frequency of G/G and G/g of STX17 related to grey coat color was 0.002 and 0.680, respectively, with the share of grey horses among the Jeju horse group at 68.2%. As for the change of coat color genes by generation, no large changes were observed in the MC1R and ASIP genes. In ECA3-inversion, the To allele that manifests Tobiano significantly decreased following the generational change (p<0.05), while the STX17 G allele related to grey coat color significantly increased following the generational change (p<0.05). It will be necessary to examine the coat color genes when selecting breeding horses so that the diversity of coat colors among the Jeju horse group can be maintained.

• Journal of Embryo Transfer
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• v.30 no.3
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• pp.149-159
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• 2015

This study was designed to know the possibility in repeat uses of elite donor cows for getting mass production of OPU-derived embryo production (OPU-IVP). Ultrasound transvaginal ovum pick-up (OPU) performed in 6 Korean native cows was aged 4 to 10 years old. The aspiration of immature oocytes for OPU derived embryo was carried out 2 times per week, and OPU-IVP of $1^{st}$ period was carried out 22~48 sessions from each donors. And the break time for OPU-IVP of $2^{nd}$ period after $1^{st}$ OPU from each donors were 2~25 months. The OPU-IVP of $2^{nd}$ period each donors conducted total 15~65 times for 2~8 months by an ultrasonographic, was guided follicular aspiration system. The average numbers of collected oocytes, grade 1 + grade 2(G1+G2) oocytes and cleavage embryo from $1^{st}$ period OPU-IVP were significantly differences between donors (p<0.05). Total collected oocytes of donor D were significantly higher compared with donors of A, B, C, E and F (average 17.0 per session vs. 11.2, 10.1, 8.5, 10.2 and 9.6; p<0.05) and also oocytes of G1+G2 were significantly higher compared with r A and D and subsequently to donors of B, C, E and F (average 7.9 and 8.5 per session vs. 5.0, 2.7, 6.0 and 1.6; p<0.05). Cleavage rate of donor D was significantly higher compared with donors of A, B, C, E and F (average 13.1 per session vs. 10.1, 9.1, 6.9, 8.9 and 6.7; p<0.05). The average numbers of OPU-IVP for $1^{st}$ period was significantly higher from donors of B, D and E than those from donors of A, C and F (average 6.5, 7.1 and 6.5 per session vs. 3.5, 4.2 and 2.8; p<0.05). The possibility investigation of $2^{nd}$ OPU-IVP was carried out after 2~25 months rest periods from $1^{st}$ period OPU session. Total average numbers of collected oocytes, cleavages and blastocyst development rates were significantly higher from $1^{st}$ period OPU compared with $2^{nd}$ period one (p<0.05). The OPU-IVP efficiency by break for more embryo production from elite cow was analysis comparing without rest of donor A, under 6 months rest period as B and over 6 months rest period as C and then the average numbers of collected oocytes, cleavages and blastocysts were significantly higher from A group (11.8, 9.5 and 5.2 per session) than those from B and C groups (7.9, 6.2 and 2.6 vs. 9.2, 7.5 and 3.9, p<0.05), and also C group was significantly higher than B group. In conclusion, $1^{st}$ period OPU-IVP was more efficient compared with $2^{nd}$ period repeated uses of donor, and the break times for additional production of embryo on donor were needed more than over 6 months after $1^{st}$ period OPU-IVP. This repeating uses of elite donor cows given more emphasis for getting the opportunity on mass production of elite cow OPU-IVP embryo should be increased G1+G2 possibility of genetic improvement of livestock within short period.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.57 no.1
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• pp.7-15
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• 2012

Agronomic characteristics of 150 adzuki bean ($Vigna$ $angularis$ W.F. Wight) germplasm collected in Korea were investigated in order to establish basic data for the improvement of crop breeding. Growth types were classified as 3 types of indeterminate type, determinate type, and semideterminate type, of which indeterminate type was the highest percentage of 96.6%. Seed color were classified as 8 colors of which dark brown took 66.5% in 150 adzuki bean germplasm. Days from seeding to maturity showed the range from 89 to 142 days with 115 days in the average and the highest frequency proportion was 37.2% of the group from 131 to 140 days, and the next was 27.3% of the group from 121 to 130 days among 150 germplasm. Pod length showed the range from 5.9 to 10.1 cm with 7.8 cm in the average and the highest frequency distribution of it was the group from 7.1 to 8.0 cm with the proportion of 45.1% and the next was the group from 8.1 to 9.0 cm with the proportion of 32.2%. Number of pods per plant showed the range from 5.0 to 132.5 pods with 28.5 pods in the average and the highest frequency distribution of it was the group from 11.0 to 20.0 pods with the proportion of 30.8% and the next was the group from 21.0 to 30.0 pods with the proportion of 28.6%. Number of seeds per pod showed the range from 5.3 to 11.3 seeds with 8.2 seeds in the average and the highest frequency proportion of it was the group from 7.1 to 8.0 seeds, which occupied 32.2% among 150 germplasm. 89.3% of 150 germplasm showed the resistance to shattering of grains at harvest period. 100 grain weight showed the range from 5.7 to 23.0 g with 12.9 g in the average and the highest frequency proportion of it was the group from 9.01 to 13.0 g, which occupied 43.3% and the next was the group from 13.01 to 17.00 g with the proportion of 28.5% of 150 germplasm.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.57 no.1
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• pp.41-50
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• 2012

The study was carried out to analyze the relationship between analysis of antioxidant activity and the level of functional components according to particle size of corn silk. Particle size was classified into 5 groups. By particle size distribution and color difference, the total phenol content and DPPH radical scavenging activity were observed. The particle sizes of corn silk were $199.17{\mu}m$, $178.27{\mu}m$, $85.48{\mu}m$, $27.4{\mu}m$ and $20.97{\mu}m$, respectively. The lightness of colored pigments was increased when the particle size was decreased. The contents of free sugar (fructose, glucose, galactose, sucrose, and maltose) of corn silk were analyzed using a HPLC. The total phenol contents by the particle sizes of corn silk were 2.01 mg/g, 2.02 mg/g, 2.06 mg/g, 2.26 mg/g and 2.26 mg/g, respectively. DPPH radical scavenging activities of samples were 21.00%, 21.75%, 22.90%, 24.35% and 23.67%, respectively. Antioxidative activities of Trolox and Fe(II) in corn silk were measured by ferric reducing antioxidant power (FRAP) assay and Trolox equivalent antioxidant capacity (TEAC) assay. TEAC values of samples were $2.36{\mu}mol$ TE / g dw, $2.81{\mu}mol$ TE / g dw, $3.20{\mu}mol$ TE / g dw, $3.36{\mu}mol$ TE / g dw, and $3.44{\mu}mol$ TE / g dw, respectively. FRAP values of samples were $11.67{\mu}mol$ Fe(II) / g dw, $12.80{\mu}mol$ Fe(II) / g dw, $13.43{\mu}mol$ Fe(II) / g dw, $13.85{\mu}mol$ Fe(II) / g dw and $15.95{\mu}mol$ Fe(II) / g dw, respectively. Total phenolic content and antioxidantive activities based on FRAP assay and TEAC assay were increased with decreasing particle size. In addition, DPPH radical scavenging activity was also increased. A significant correlation was also noted between DPPH radical scavenging activities and the content of phenolic compounds.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.62 no.4
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• pp.352-360
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• 2017

This study evaluated the quality characteristics, polyphenolic compounds, and radical scavenging activity of cooked-rice added to commercially available mixed grains. L-value of cooked-rice with various mixed grains decreased compared to that of cooked-white rice; however, a- and b-values increased. Hardness and elasticity of cooked-rice added to various mixed grains were significantly lower in the pressure cooker compared to the electric cooker. There was no significant difference in adhesiveness and stickiness between rice from the electric cooker and pressure cooker. Total polyphenol and flavonoid contents of cooked-rice added to various mixed grains were significantly increased. The average total polyphenol content of cooked-rice added to various mixed grains cooked in an electric cooker and pressure cooker were $16.50{\pm}3.86$ and $15.88{\pm}3.52mg$ gallic acid equivalent /100 g, and flavonoid contents were $1.58{\pm}0.00$ and $1.55{\pm}0.02mg$ catechin equivalents/100 g, respectively. The average of 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity was $9.27{\pm}2.62$ and $8.72{\pm}2.41mg$ trolox equivalent (TE)/100 g, and 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity was $22.89{\pm}4.60$ and $23.07{\pm}4.49mg$ TE/100 g for cooked-rice added to various mixed grains cooked in an electric cooker and pressure cooker, respectively. Phenol content and radical scavenging activity of cooked rice was in proportion to the amount of added grains, such as brown rice, colored rice, barley, soybean, and sorghum.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.62 no.4
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• pp.293-303
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• 2017

To elucidate the physiological responses of rice plants to the essential mineral silicon (Si), we assessed the effects of treatments with Si, nitrogen ($NH_4NO_3$; ammonium nitrate), and calcium ($CaCl_2$; calcium chloride), independently or in combination on mineral uptake rates and levels of the hormones abscisic acid (ABA), gibberellin ($GA_1$) and jasmonic acid (JA). We found that nitrogen and calcium uptake was inhibited by Si application. However, solo application of nitrogen or calcium did not affect Si uptake. Compared to the untreated plants, the application of Si, $NH_4NO_3$ or $CaCl_2$ increased the endogenous hormone levels in treated plants. In particular, the concentrations of $GA_1$ and JA increased significantly after the application of Si or $NH_4NO_3$. The level of $GA_1$ observed after a treatment (solo or combine) with Si, and $NH_4NO_3$ was higher than that of the control. By contrast, independent application of $CaCl_2$ or a combined treatment with Si and $CaCl_2$ did not alter $GA_1$ levels. The highest level of $GA_1$ was present in plants given a combination treatment of Si and $NH_4NO_3$. This effect was observed at all time points (6 h, 12 h and 24 h). Endogenous JA contents were higher in all treatments than the control. In particular, a combination treatment with Si and $NH_4NO_3$ significantly increased the JA levels in plants compared to other treatments at all time points. A small increase in JA levels was observed after 6 h in plants given the $CaCl_2$ treatment. However, JA levels did not differ between plants given a $CaCl_2$ treatment and controls after 12 h or 24 h of exposure. We conclude that treatment with $CaCl_2$ alone does not affect endogenous JA levels in the short term. Endogenous ABA contents did not show any differences among the various treatments.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.62 no.4
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• pp.333-345
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• 2017

The potato tuber is known as a rich source of essential nutrients, used throughout the world. Although potato-breeding programs share some priorities, the major objective is to increase the genetic potential for yield through breeding or to eliminate hazards that reduce yield. Glycoalkaloids, which are considered a serious hazard to human health, accumulate naturally in potatoes during growth, harvesting, transportation, and storage. Here, we used the AMMI (additive main effects and multiplicative interaction) and GGE (Genotype main effect and genotype by environment interaction) biplot model, to evaluate tuber yield stability and glycoalkaloid content in six potato cultivars across three locations during 2012/2013. The environment on tuber yield had the greatest effect and accounted for 33.0% of the total sum squares; genotypes accounted for 3.8% and $G{\times}E$ interaction accounted for 11.1% which is the nest highest contribution. Conversely, the genotype on glycoalkaloid had the greatest effect and accounted for 82.4% of the total sum squares), whereas environment and $G{\times}E$ effects on this trait accounted for only 0.4% and 3.7%, respectively. Furthermore, potato genotype 'Superior', which covers most of the cultivated area, exhibited high yield performance with stability. 'Goun', which showed lower glycoalkaloid content, was the most suitable and desirable genotype. Results showed that, while tuber yield was more affected by the environment, glycoalkaloid content was more dependent on genotype. Further, the use of the AMMI and GGE biplot model generated more interactive visuals, facilitated the identification of superior genotypes, and suggested decisions on a variety of recommendations for specific environments.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.64 no.4
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• pp.395-405
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• 2019

A recent assessment by the Intergovernmental Panel on Climate Change projected that the global average surface temperature will increase by a value 1.5℃ from 2030 to 2052. In this study, we used a temperature gradient chamber that mimicked field conditions to evaluate the effect of increased air temperature on phenology, yield components, protein content, and oil content, to assess soybean growth. In 2017 and 2018, 'Deawonkong', 'Pungsannamulkong', and 'Deapungkong' cultivars were grown in three temperature gradient chambers. Four temperature treatment groups were established by dividing the rows along temperature regimes: ambient temperature + 1℃ (aT+1), ambient temperature + 2℃ (aT+2), ambient temperature + 3℃ (aT+3), ambient temperature + 4℃ (aT+4). Year, cultivar, and temperature treatments significantly affected yield components and seed yield. In 2017, the flowering stage of 'Deawon' and 'Pungsannamul' cultivars in the aT+4 group was delayed compared to the flowering stage of those in the aT+1 group. In 2018, the flowering stage of 'Deawon' and 'Pungsannamul' was delayed at all temperature gradients, owing to high temperature stress, whereas 'Deapung' was regularly flowering in 2017 and 2018. The duration of the grain filling period was six days shorter in 2018 than in 2017 because of high temperature stress. The total number of pods per ㎡ for 'Deawon' and 'Pungsannamul' was 48.8 and 41.5% lower in 2018 than in 2017, respectively, whereas 'Deapung' increased by 6.3%. The 100-seed weight of 'Deawon' and 'Deapung' was 29.2 and 32.1% lower, respectively. However, 'Pungsannamul' decreased by 14.7%. The protein and oil content was lower during the grain filling period in 2018 than in the same period in 2017 because of high temperature stress. In contrast, the oil content in 'Deapung' was higher in 2018 than in 2017. Our results showed that increased temperature during the grain filling period was significantly and negatively correlated with pod number, 100-seed weight, protein content, and oil content.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.64 no.4
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• pp.406-413
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• 2019

The purpose of this research was to analyze the effect of different sowing times on the flowering and maturing of major soybean cultivars by varying day length and temperature in the central plain region. The average of growth period and cumulative temperature in five test cultivars by sowing times were 121 days and 2,972℃ on June 1, respectively and gradually decreased to 85 days, 2,042℃, respectively on July 20. Analysis of the flowering response according to the sowing times showed that flowering was greatly influenced by the decrease of photoperiod until the sowing on July 10, and the minimum number of days for flowering were 27 days, 36 days, respectively in early and mid-rate maturing type in the central plain region. Daepung 2 is classified to the same ecotype with Daewonkong, the total number of growing days was not different between two cultivars, but ripening period (R2-R6) was longer by 5 days and yield was higher by 11% in Daepung 2. The maturity rate was also high and safe enough to maintain more than 90% through the entire sowing times. This ecological characteristic can be usefully applied as a section index for breeding environmental stress resistant and high yielding soybean varieties. The yield of 4 domestic cultivars (except TI196944) sowing on July 20 were 85~92% levels compared to sowing on June 20.

• Microbiology and Biotechnology Letters
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• v.38 no.2
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• pp.168-176
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• 2010

Acellular dermal matrix (ADM), produced by decellularization from human cadaveric skin, has been used for various biomedical applications. A manufacturing process for ADM ($SureDerm^{TM}$) using tri-n-butyl phospahate (TnBP) and deoxycholic acids as the decellularization solution has been developed. The manufacturing process for $SureDerm^{TM}$ has 70% ethanol treatment and ethylene oxide gas sterilization for inactivating infectious microorganisms. The purpose of this study was to examine the efficacy of the 70% ethanol treatment, decellularization process using 0.1% TnBP and 2% deoxycholic acids, and EO gas sterilization process in the inactivation of viruses. A variety of experimental model viruses for human pathogens, including the human immunodeficiency virus type 1 (HIV-1), bovine herpes virus (BHV), bovine viral diarrhoea virus (BVDV), hepatitis A virus (HAV), and porcine parvovirus (PPV) were all selected for this study. Enveloped viruses such as HIV-1, BHV, and BVDV were effectively inactivated to undetectable levels by 70% ethanol treatment. However HAV and PPV showed high resistance to 70% ethanol treatment with the log reduction factors of 1.85 and 1.15, respectively. HIV-1, BHV, and BVDV were effectively inactivated to undetectable levels by decellularization process. All the viruses tested were completely inactivated to undetectable levels by EO gas treatment. The cumulative log reduction factors of HIV-1, BHV, BVDV, HAV, and PPV were $\geq12.71$, $\geq18.08$, $\geq14.92$, $\geq6.57$, and $\geq7.18$, respectively. These results indicate that the production process for $SureDerm^{TM}$ has a sufficient virus-reducing capacity to achieve a high margin of the virus safety.