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Effects of Selection Criteria for Eco-Friendly Agricultural Products on Purchase Intention (친환경농산물 선택기준이 구매의도에 미치는 영향 : 소비자 태도와 신뢰의 매개, 조절효과를 중심으로)

  • Kim, Mi-Song;Kim, Dong-Hwan;Lee, Gi-Hwang;Youn, Myoung-Kil
    • Journal of Distribution Science
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    • v.11 no.12
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    • pp.71-81
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    • 2013
  • Purpose - This study investigated the effects of consumers' selection criteria for environment-friendly agricultural products on purchase intention and the effects of consumers' attitudes and the reliability of environment-friendly agricultural products on purchase intention by using the theory of planned behavior. Subjective norms of variables of behavioral intention, attitudes toward behavior and control of the behavior were used to create selection criteria, consumers' attitudes and reliability of environment-friendly agricultural products. The study investigated the effects of consumers' selection criteria, attitudes, and reliability of environment-friendly agricultural products on purchase intention constructing models and hypotheses of mediation and moderation between selection criteria for agricultural products and purchase intention by consumers' attitudes and reliability. Research design, data, and methodology - The findings were as follows: first, consumers' selection criteria for environment-friendly agricultural products had a significantly affirmative influence upon purchase intention. Health was the most important factor of selection criteria convenience was more important than quality and familiarity was next. Consumers' attitudes and trust had a significant influence on purchase intention. Second, testing showed that consumers' attitude and trust partially mediated selection criteria: sub-factors and purchase intention were important in selection criteria. Third, testing showed that consumers' attitude and trust had a significant moderation effect between selection criteria and purchase intention. In the test of the moderation effect between sub-factors of selection criteria and purchase intention, consumers' attitude had a significantly positive influence upon health, convenience, and familiarity, and had no significant influence upon quality and purchase intention. Consumers' trust had no significant influence upon health, convenience, and quality. Results - The study provided several theoretical implications: first, an empirical analysis was undertaken with selection criteria for environmental-friendly agricultural products, consumers' attitude, and trust to investigate subjective norms, attitude toward behavior and control of behavior based on the theory of planned behavior. Second, this study investigated both the mediation effect and moderation effect of consumers' subjective norms on attitudes toward behavior, the mediating effects of perceived behavior control and changes of behavioral intention depending upon size and direction of the variables. This study also provided several practical implications. Conclusions - First, consumption of environment-friendly agricultural products did not increase despite rapid increase of production therefore, promotion of consumption and distribution was needed considering the supply and demand of the products. Second, definite standards for selection criteria were suggested to build up consumers' attitude and trust. Consumers' attitude could be improved by factors including the brand of environment-friendly agricultural products, consistent quality, solving physiological problems caused by adverse effects of environmental problems, supplementary approaches, treatment of adverse effects by eating food, and the development and supply of products in accordance with changes of lifestyle. Finally, consumers' demand for sub-factors of selection criteria could be much higher than health, convenience, and quality of the products. Therefore, a process was needed that could continuously check consumers' needs for the products. Limitations were described at the end of the study.

Perspectives on the Current Condition and Landscape Management Status of the Dangsan Forests in Sungnam-ri, Wonju (원주시 성남리 당산숲의 현황 및 경관관리 실태 고찰)

  • Choi, Jai-Ung;Kim, Dong-Yeob;Lee, Sang-Hwa;Kim, Sung-Gi
    • Journal of the Korean Institute of Landscape Architecture
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    • v.35 no.3
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    • pp.82-91
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    • 2007
  • Rural areas dominate country landscape, where the population is rich in traditional and natural heritage. Dangsan forests and Rural community forests (RCF) have been maintained by local residents for hundreds of years. However, many of these forests have been disturbed, and only small amount of Dangsan forests and the RCF remain due to the efforts of local residents. Recently, the remnants of Dangsan forests and RCF have been regarded as living traditional landscapes representing the cultural and rural background of Korea. But the value of Dangsan forests and the RCFs has not been recognized by many. This study aims to understand the characteristics and management status of Dangsan forests in Sungnam-ri, Wonju. Sungnam-ri has four villages, each of which is bordered by a Dangsan forest at the riparian buffer. Sungnam-ri has been selsected as a site for a rural village development project by the Ministry of Agriculture & Forestry of Korea. Sunghwanglim (the Upper Dangsan forest) is designated as Natural Monument No.93 and entrance is limited in this area. The size of Sunghwanglim is $21,133m^2$, and the major tree species are Kalopanax pictus (Dangsan tree, Divine tree), Pinus densiflora, Abies holophylla, Acer triflorum, Ulmus davidiana, Fraxinus rhynchophylla and Quercus serrata. In total, 124 trees with more than 40 cm in DBH are at this site. As for cultural activities, the Dangsan festival is held on April 8th and September 9th of the lunar calendar at shrines in the forests. Although other Dangsan forests are smaller than Sunghwanglim, they also have similar cultural activities. The landscape of the Dangsan forests have been managed for several hundred years by the local people, and they intend to continue maintaining the Dangsan forests for the purpose of festivals and other cultural activities. The findings in this study suggest that Dangsan forests have the potential for the development of rural villages and for the improvement of cultural and natural landscapes in Korea.

Evaluation of Optimum Mix Proportion and Filling Performance of High-fluidity Concrete for SCP Module charging (SCP 모듈 충전용 고유동 콘크리트의 최적배합 도출 및 채움성능 평가)

  • Park, Gi-Joon;Kim, Sung-Wook;Park, Jung-Jun;Lee, Dong-Gyu
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.18 no.3
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    • pp.452-459
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    • 2017
  • In recent years, to reduce self-weight of structural elements, the use of SCP (Steel Concrete Plate) instead of prestressed concrete is increasing. Because SCP has a complicated sectional shape and includes a large number of studs, the use of high-fluidity concrete is required. Therefore, in this study, to prevent the restrained shrinkage behavior by the stud, the effects of using an expansive agent (EA) and shrinkage reducing agent (SRA) were investigated, and the optimal mixture proportions to maximize the filling capacity were determined based on the fine aggregate ratio. The test results indicated that the combined use of EA and SRA exhibited the smallest shrinkage. The ratio of the crushed sand and washed sea sand was determined to be 5:5, and the proper fine aggregate ratio was found to be 55.6%, because when the ratio was too high, a decrease in strength and an increase in shrinkage strain were expected. The high-fluidity concrete effectively filled the large-sized SCP module.

Single and 28-day repeated dose toxicity studies of botulinum toxin type A in mice and rats (마우스 및 랫드에서 botulinum toxin type A의 단회 및 28일 반복투여 독성시험)

  • Jeon, Tae-Won;Kim, Ji-Young;Hyun, Sun-Hee;Kim, Nam-Hee;Lee, Sang-Kyu;Kim, Chun-Hwa;Woo, Hee-Dong;Yang, Gi-Hyeok;Jung, Hyun-Ho;Jeong, Tae-Cheon
    • Korean Journal of Veterinary Research
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    • v.43 no.1
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    • pp.57-66
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    • 2003
  • Single and 28-day repeated dose toxicity studies of botulimnn toxin type A were carried out in ICR mice and Sprague-Dawley rats, respectively. In the single dose toxicity study, botulinwn toxin was injected intraperitoneally to male and female mice at a single dose of 40, 59, 89 133 and 200 ng/10 ml saline/kg. All animals died from 59 ng/kg group. Some clinical signs, such as decrease in locomotor activity, dyspnea, prone position and ptosis, were observed in most of both sexes from 59 ng/kg group, but no signs were seen in all animals at 40 ng/kg group. The results showed that the median lethal dose of botulinum toxin might be in the range of 40-59 ng/kg in both sexes. In the repeated dose toxicity study, the test material was administered intradermally for 28 days at doses of 0 (vehicle-treated control), 1.25, 2.5, 5.0 and $10.0ng/head/50{\mu}{\ell}$ saline in male and female rats. No test material-related changes were noted in survivals, clinical signs, food and water consumptions and gross finding in any group. Botulinum toxin treatment significantly decreased the body weight gain rate in male of 5.0 ng/head group and over and in female of 10.0 ng/head group compared to vehicle-treated control. One or more relative organ weights (i.e., spleen, thymus, liver and kidney) were increased significantly from 5.0 ng/head group compared to vehicle-treated control in both sexes. Serum biochemistry revealed increases in aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatine phosphokinase, total protein and albumin in male, and increases in AST and ALT and decreases in $K^+$ and $Cl^-$ in female without dose-pendent manners. In the histopathological study, physical stimulation by needle caused slight inflammations of dennis. In addition, botulinum toxin treatment induced denervation of nerve cell and disuse of muscle, resulting in atrophy of skeletal muscle in both sexes from 2.5 ng/head group. When the antibodies to toxin were determined in all animals, a significant increase in serum antibodies was observed from 5.0 ng/head group. The results showed that the NOAEL of botulinum toxin might be 1.25 ng/head for 28-day repeated dose toxicity in rats.

Analysis of Effects from Traffic Safety Improvement on Roadways using C-G Method (비교그룹방법을 이용한 교통안전 시설물 설치 효과 분석)

  • Lee, Dong-Min;Kim, Do-Hun;Song, Gi-Seop
    • Journal of Korean Society of Transportation
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    • v.29 no.3
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    • pp.31-40
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    • 2011
  • Generally, inappropriate driving conditions including geometric, traffic environmental, and driver psychological problems may be critical reasons of traffic accidents. Under this circumstance, various types of facilities have been installed to improve traffic safety by itself or as a set consisting of several other traffic safety facilities. In general, traffic accidents occur by several reasons combined rather than only a single reason, and thus the safety effect of the safety facilities cannot be simply analyzed with only a single improvement. For the study, traffic accident data on the roadway segments of interest are collected along with field survey data. For the analysis, various alternative analysis methods were evaluated in terms of assessing accident reduction from various types of traffic safety improvements. Among the alternative methods tested including simple before-and-after evaluation method, before-and-after evaluation yoked comparison, and Comparison Group (C-G) method, it was found that the C-G method is the most effective method for analyzing the traffic safety improvement effect. Adopting the C-G method, both single and multiple safety improvements were analyzed. The results from this study can potentially be applied to decide the best type of treatments to improve traffic safety as well as to measure the accident reduction effects from the treatments.

A Study on Constructive Method and the Practical use of Marine GIS (해양 GIS 구축 및 활용 방안 연구)

  • Lee, Gi-Chul;Suh, Sang-Hyun;Cho, Seung-Rae;Han, Chang-Seuk;Park, Chang-Ho;Roh, Hong-Seung;Kim, Eun-Hyung;Kim, Jin-Hoo;Park, Jong-Hwa;Suh, Young-Sang
    • Journal of the Korean Association of Geographic Information Studies
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    • v.9 no.2
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    • pp.126-143
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    • 2006
  • The main objective of this study is to determine the methodology to increase the practical use of future Marine GIS based on the user's point of view of the analysis of maritime & fishery government agencies's roles. GIS related job duty of two Busan District offices of MOMAF(Ministry of Maritime and Fisheries), NFRDI(National Fisheries Research & Development Institute), Korea Institute of Maritime and Fisheries Technology and marine & fisheries local government offices of Busan Metropolitan city were analyzed for the establishment of MGIS, future extended service targeting user's increased practical utilization. Several suggestions in the area of production mechanism and technical policy for marine geographic information among the government agencies are developed to settle down successfully MGIS in Busan. Furthermore, 10 fields of Marine Geographic issues are rearranged for intending the desirable system and service.

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IL-12 Production and Subsequent Natural Killer Cell Activation by Necrotic Tumor Cell-loaded Dendritic Cells in Therapeutic Vaccinations

  • Kim, Aeyung;Kim, Kwang Dong;Choi, Seung-Chul;Jeong, Moon-Jin;Lee, Hee Gu;Choe, Yong-Kyung;Paik, Sang-Gi;Lim, Jong-Seok
    • IMMUNE NETWORK
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    • v.3 no.3
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    • pp.188-200
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    • 2003
  • Background: Immunization of dendritic cells (DCs) pulsed with tumor antigen can activate tumor-specific cytotoxic T lymphocytes (CTL) that are responsible for protection and regression. In this study, we examined whether the uptake of necrotic tumor cells could modulate DC phenotypes and whether the immunization of necrotic tumor cell-loaded DCs could elicit efficient tumor specific immune responses followed by a regression of established tumor burdens. Methods: We prepared necrotic tumor cell-pulsed DCs for the therapeutic vaccination and investigated their phenotypic characteristics, the immune responses induced by these DCs, and therapeutic vaccine efficacy against colon carcinoma in vivo. Several parameters including phagocytosis of tumor cells, surface antigen expression, chemokine receptor expression, IL-12 production, and NK as well as CTL activation were assessed to characterize the immune response. Results: DCs derived from mouse bone marrow efficiently phagocytosed necrotic tumor cells and after the uptake, they produced remarkably increased levels of IL-12. A decreased CCR1 and increased CCR7 expression on DCs was also observed after the tumor uptake, suggesting that antigen uptake could induce DC maturation. Furthermore, co-culturing of DCs with NK cells in vitro enhanced IL-12 production in DCs and IFN-${\gamma}$ production in NK cells, which was significantly dependent on IL-12 production and cell-to-cell contact. Immunization of necrotic tumor cell-loaded DCs induced cytotoxic T lymphocytes as well as NK activation, and protected mice against subsequent tumor challenge. In addition, intratumoral or contra-lateral immunization of these DCs not only inhibited the growth of established tumors, but also eradicated tumors in more than 60% of tumor-bearing mice. Conclusion: Our data indicate that production of IL-12, chemokine receptor expression and NK as well as CTL activation may serve as major parameters in assessing the effect of tumor cell-pulsed DC vaccine. Therefore, DCs loaded with necrotic tumor cells offer a rational strategy to treat tumors and eventually lead to prolonged survival.

EFFECT OF THIRD MOLAR ON POSTOPERATIVE INFECTION AFTER REDUCTION OF THE MANDIBULAR ANGLE FRACTURE (하악 제3대구치가 하악 우각부골절 정복술후 감염에 미치는 영향에 관한 연구)

  • Choi, Moon-Gi;Min, Seung-Ki;Lee, Dong-Keun;Oh, Seung-Hwan
    • Maxillofacial Plastic and Reconstructive Surgery
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    • v.23 no.3
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    • pp.217-225
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    • 2001
  • Any fracture passing through the socket of a teeth is compounded intraorally, even if the fracture is not displaced and the tooth is firm in its socket. Before the advent of antibiotic therapy the danger of infection in a compounded fracture posed severe problems in treatment. Infection is reduced by antibiotic therapy but prolonged use of antibiotics is not justified in an attempt to save a tooth which might eventually be sacrificed. There is still controversy in the management of third molar in mandibualr angle fracture, particulary in regard to their retention or removal at the time of fracture treatment. So we surveyed the 159 patients who were treated with open reduction of mandibular angle fracture containing third molar in fracture line, and compared with the postoperative infection rate depending on time intervals between injury and operation, eruption state of third molar, non-extraction or extraction of third molar related to eruption state, non-extraction or extraction of third molar related to condiition of third molar and its surrounding periodontium and were to propose treatment guidline of third molar in mandibular angle fracture The results obtained were as follows : 1. There were no statistical significance between the time from injury to operation and postoperative infection. 2. There were no statistical significance between eruption state of third molar and postoperative infection. 3. In case of retention of the third molar, there were no statistical significance between eruption state of third molar and postoperative infection, but in case of extraction, postoperative infection was high rate in complete impacted cases. 4. There were no statistical significance between non-extraction or extraction of third molar and postoperative infection depending on condition of third molar. There are no difference in infection rate statistically according to the time from injury to operation, eruption state and condition of third molar, but retention of third molar revealed lowered infection rate in completely impacted cases. By terms of the manegement of third molar, we should extract or preserve third molar in the line of the mandibular angle fracture according to possibility of infection.

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The Tunnel Lane Positioning System of a Autonomous Vehicle in the LED Lighting (LED 조명을 이용한 자율주행차용 터널 차로측위 시스템)

  • Jeong, Jae hoon;Lee, Dong heon;Byun, Gi-sig;Cho, Hyung rae;Cho, Yoon ho
    • The Journal of The Korea Institute of Intelligent Transport Systems
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    • v.16 no.1
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    • pp.186-195
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    • 2017
  • Recently, autonomous vehicles have been studied actively. There are various technologies such as ITS, Connected Car, V2X and ADAS in order to realize such autonomous driving. Among these technologies, it is particularly important to recognize where the vehicle is on the road in order to change the lane and drive to the destination. Generally, it is done through GPS and camera image processing. However, there are limitations on the reliability of the positioning due to shaded areas such as tunnels in the case of GPS, and there are limitations in recognition and positioning according to the state of the road lane and the surrounding environment when performing the camera image processing. In this paper, we propose that LED lights should be installed for autonomous vehicles in tunnels which are shaded area of the GPS. In this paper, we show that it is possible to measure the position of the current lane of the autonomous vehicle by analyzing the color temperature after constructing the tunnel LED lighting simulation environment which illuminates light of different color temperature by lane. Based on the above, this paper proposes a lane positioning technique using tunnel LED lights.

Mutation Analysis of Synthetic DNA Barcodes in a Fission Yeast Gene Deletion Library by Sanger Sequencing

  • Lee, Minho;Choi, Shin-Jung;Han, Sangjo;Nam, Miyoung;Kim, Dongsup;Kim, Dong-Uk;Hoe, Kwang-Lae
    • Genomics & Informatics
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    • v.16 no.2
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    • pp.22-29
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    • 2018
  • Incorporation of unique barcodes into fission yeast gene deletion collections has enabled the identification of gene functions by growth fitness analysis. For fine tuning, it is important to examine barcode sequences, because mutations arise during strain construction. Out of 8,708 barcodes (4,354 strains) covering 88.5% of all 4,919 open reading frames, 7,734 barcodes (88.8%) were validated as high-fidelity to be inserted at the correct positions by Sanger sequencing. Sequence examination of the 7,734 high-fidelity barcodes revealed that 1,039 barcodes (13.4%) deviated from the original design. In total, 1,284 mutations (mutation rate of 16.6%) exist within the 1,039 mutated barcodes, which is comparable to budding yeast (18%). When the type of mutation was considered, substitutions accounted for 845 mutations (10.9%), deletions accounted for 319 mutations (4.1%), and insertions accounted for 121 mutations (1.6%). Peculiarly, the frequency of substitutions (67.6%) was unexpectedly higher than in budding yeast (~28%) and well above the predicted error of Sanger sequencing (~2%), which might have arisen during the solid-phase oligonucleotide synthesis and PCR amplification of the barcodes during strain construction. When the mutation rate was analyzed by position within 20-mer barcodes using the 1,284 mutations from the 7,734 sequenced barcodes, there was no significant difference between up-tags and down-tags at a given position. The mutation frequency at a given position was similar at most positions, ranging from 0.4% (32/7,734) to 1.1% (82/7,734), except at position 1, which was highest (3.1%), as in budding yeast. Together, well-defined barcode sequences, combined with the next-generation sequencing platform, promise to make the fission yeast gene deletion library a powerful tool for understanding gene function.