• Biomolecules & Therapeutics
• /
• 제11권3호
• /
• pp.169-173
• /
• 2003

Alkylthioureido-1,3-propandiols (KY353X series) were synthesized and evaluated as inhibitors for neutral sphingomyelinase (N-SMase). To examine whether KY353X series inhibit N-SMase, we purified the N-SMase from bovine brain. The N-SMase was partially purified by sequential chromatographies of DEAE-Cellulose anionic exchange and phenyl-5PW hydrophobic HPLC. These seqeuntial procedures for N-SMase resulted in a 67-fold purification and excluded other isoforms of SMase. Based on in vitro assay, KY353X series inhibited N-SMase activity in time, concentration-dependent manners and completely inactivated N-SMase at 50 $\mu$M. In particular, KY3535 and KY3536 inhibited more effectively than the others. To further determine the .inhibitory pattern, a Dixon plot was constructed, to showing that the inhibition by KY3535 and KY3536 were competitive. The inhibition constant (Ki) of KY3535 and KY3536 was 1.7 $\mu$M and 2.5$\mu$M in 100 mM Tris-HCl buffer, pH 7.0, respectively.

• 간호행정학회지
• /
• 제8권2호
• /
• pp.221-238
• /
• 2002

Purpose: The purpose of this study was to develop and test the validity of the standardized Korean nomenclature of 260 Nursing Outcomes Classification(NOC) developed by Johnson and Mass at University of Iowa in 2000. Method: The four phases of the study were: (1) translation of the NOC into Korean by the Research Team, (2) nine nursing professors and nurses with various clinical backgrounds reviewed each nomenclature taking into consideration of definitions and outcome indicators. The modified Delphi method was used to determine the most appropriate nomenclature for each term, (3) 307 Clinical expert nurses more than three years field experiences were given a questionnaire to rate each Korean nomenclature using a 5 point Likert scale ranging from very inappropriate to very appropriate, and (4) final accordance of Korean Nomenclature. Result: The team determined that 260 Korean nomenclature was appropriately named. because the mean validity score of 260 outcomes was 3.90 and each of 260 Korean nomenclature had a score more than 3.00. Conclusion: Korean nomenclature of NOC can be used as a standardized language of nursing result in a computerized nursing information system.

• Mycobiology
• /
• 제33권1호
• /
• pp.15-18
• /
• 2005

Macrolepiota procera, one of edible mushrooms belongs to Agaricaceae of Basidiomycota, has a good taste and good medicinal value. As a preliminary study for the development of artificial cultivation method of edible mushroom, cultural characteristics of M. procera was investigated on various culture media under different environmental conditions. Mycelial growth was compared on culture media composed of various carbon and nitrogen sources, and C/N ratios. The optimal conditions for the mycelial growth were $30^{\circ}C$ and pH 7. M. procera showed the rapid mycelial growth in the PDA media. The optimal carbon and nitrogen sources were maltose and glycine, respectively. The optimum C/N ratio was about 10 : 1 in case that 1% glucose was supplemented to the basal media as carbon source.

• Mycobiology
• /
• 제36권2호
• /
• pp.88-92
• /
• 2008

Vegetative growth of four different strains of Hericium erinaceus was observed. The temperature suitable for optimal mycelial growth was determined to be $25^{\circ}C$, with growth observed in the extend temperature range of $20{\sim}30^{\circ}C$. The different strains of this mushroom showed distinct pH requirements for their optimum vegetative growth, with the most favorable growth observed at pH 6. Considering vegetative mycelial growth, PDA, YM, Hennerberg, Hamada, and Glucose peptone were the most favorable media, and Czapek Dox, Hoppkins, Glucose tryptone, and Lilly were the most unfavorable media for these mushroom strains. With the exception of lactose, most of the carbon sources assayed demonstrated favorable vegetative growth of H. erinaceus. For mycelial growth, the most suitable nitrogen source was alanine and the most unsuitable was histidine. Oak sawdust medium supplemented with $10{\sim}20%$ rice bran was the best for mycelial growth of the mushroom.

• 아동학회지
• /
• 제26권5호
• /
• pp.297-309
• /
• 2005

This study investigated the relationship between young children's narrative representation of mother and their self-concept. Subjects were 120 children of 5 years 01d(60 boys and 60 girls) at three nursery centers located in Daejon city. The children's narrative representation of their mother was examined using the tool of Ryu & Lee(200l) based upon MSSB made by Bretherton et al(1990). And the children's self-concept was examined using the tool of Lim(1995) based upon 'I feel${\cdots}$Me feel' made by Bently & Yeatts(1974). The data were analysed by frequency, two independent t-test and Pearson's correlation with SPSS program. The young children's narrative representation of mother was positive. And there were significant correlations between the young children's narrative representations of mothers and their self-concept.

• 한국재료학회지
• /
• 제7권11호
• /
• pp.929-933
• /
• 1997

에폭시 수지 DGEBA/MDA/GN계의 트링 열화에 미치는 전압과 온도의 영향에 대한 고찰을 통하여 고분자 절연재료의 절연파괴 거동을 연구하였다. 전압 인가시간에 따라 트리의 성장속도은 역S자의 형태로 나타났다. 인가전압과 온도가 증가함에 따라 트리의 성장속도는 증가하였으며 트리형상의 복잡함, 즉 국부적인 트링열화의 정도는 인가전압이 감소하거나 온도가 증가한 경우에 높게 나타났다. 절연파괴 강도는 유리 전이온도 이상에서 급격히 감소하였다.

• 생약학회지
• /
• 제15권2호
• /
• pp.61-73
• /
• 1984

To investigate antitumor component of Lyophyllum decastes, the aqueous extract of its shake-cultured mycelia was subjected to antitumor test against sarcoma 180 cells implanted in ICR mice. The extract showed an inhibition ratio of 65.4% and was found to consist of a polysaccharide moiety and a protein moiety. After purification with DEAE-Sephadex A-50 ion exchange chromatography, Fraction D showed the highest inhibition ratio of 75.7%. The antitumor constituent was examined for immunoaccelerating activity and was found to increase macrophage accumulation in the peritoneal cavity and plaque forming cells of the spleen cells. It was named lyophyllan after the genus name.

• 아동학회지
• /
• 제29권1호
• /
• pp.1-14
• /
• 2008

This study reviewed communication between mothers and teachers of early childhood educational institutions and the effect of communication on mothers' satisfaction with the institution and on parenting stress. Results showed that (1) the most discussed topic pertained to children's friendships at the educational institutions. (2) Attitudes during communication were comparatively favorable. (3) Increased communication of mothers with the teachers showed increased satisfaction with the early childhood educational institution. (4) Mothers with positive understanding of communication and understanding of teachers showed higher satisfaction rate towards the early childhood educational institution. (5) Mothers showing higher satisfaction towards early childhood educational institution and positive attitudes during communication with teachers exhibited less parenting stress.

• 대한간호학회지
• /
• 제46권5호
• /
• pp.687-696
• /
• 2016

Purpose: The purpose of this study was to define and clarify the concept of volition for health behavior. Methods: For this study the process of Walker & Avant's concept analysis was used. Seventeen studies from electronic data basses met criteria for selection. Results: Volition can be defined by the following attributes: 1) planning, 2) maintenance of self-efficacy, and 3) self regulation. The antecedents of volition consisted of: 1) risk awareness, 2) outcome expectation, 3) perceived self-efficacy, and 4) social support. The consequences occurring as a result of volition were: 1) prevention of disability and complications, 2) improvement of functional ability, and 3) enhencement of quailty of life. Conclusion: Definition and attributes of volition identified by this results can be applied to develop measurements and intervention programs for chronic patients health behavior.

• 대한미생물학회지
• /
• 제34권6호
• /
• pp.595-598
• /
• 1999