• Journal of Life Science
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• v.19 no.9
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• pp.1284-1288
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• 2009

To produce high quality fish oil products, additional deodorization experiments on purified fish oil from squid using columns filled with citric acid or gluconic acid were performed. A deodorization effect on the fish oil was observed on both the citric acid and gluconic acid columns. These effects were more efficient on the columns packed with 3 g of organic acid than those with 1 g or 2 g of organic acid. In addition, a better effect was observed in the column packed with gluconic acid than that with citric acid. Peroxide value (POV) and acid value (AV) of the sample treated with citric acid was the as same as the non-treated sample. However, POV and AV of the sample treated with gluconic acid were about 10% higher than the non-treated sample. Contents of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) of the samples treated with citric acid or gluconic acid columns were about 0.5% higher than the non-treated sample. In conclusion, deodorization of squid fish oil by organic acid could be an efficient method to produce high quality fish oil products.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.48 no.2
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• pp.128-136
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• 2012

Hybrid surveys using hydroacoustic and fish sampling gears such as trammel net, trap were conducted to investigate total biomass, distribution and dominant fish species of the demersal fishes in the marine ranching area (MRA) of Jeju. Four surveys were carried out in June, August, October and November using 38kHz quantitative echo sounder. Catch data using trammel net and trap were used to calculate biomass and to examine dominant fish species. Fish schools were mainly detected in the waters of 20 meters below and around Chagwido waters. By the result of fishing experiments, fish species living in MRA of Jeju were about 40 species, dominant fish species of a detectable fish such as Family Sparidae and Family Monacanthidae etc. were identified 59.4~68.8% of total biomass. Based on the hydroacoustic data, fish length-weight function and target strength information, mean density of the demersal fish estimated were as follows; 0.88g/$m^2$ on June, 1.12g/$m^2$ on August, 1.35g/$m^2$ on October and 1.18g/$m^2$ on November. An estimated average biomass in MRA of Jeju was founded 20.5 tons in 2007, 20.6 tons in 2008, 23.0 tons in 2009, 25.9 tons in 2010. The results showed that biomass of MRA is getting increased slowly. Therefore the hybrid survey method using hydroacoustic and fish sampling gears is assured an effective biomass survey in the waters of mixed species.

• Journal of Life Science
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• v.16 no.7 s.80
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• pp.1169-1173
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• 2006

Extracted fractions of the green seaweed Ulva lactuca were studied to verify the cytotoxicity and immunostimulating activity. The fractions from the ethanol extract of U. lactuca were prepared by the systematic extraction procedure with solvents such as hexane, ethyl ether, methanol, butanol and $H_2O$. The cytotoxic effects of U. lactuca fractions against human leukemia cell line U937, mouse neuroblastoma cell line (NB41A3), human hepatoma cell line (HepG2) and rat glioma cell line (C6) were investigated. Ethyl ether fraction showed the highest cytotoxicity against all four cell lines tested. In addition, $H_2O$ fraction also showed relatively high cytotoxicity. Dose dependent patterns were observed on all four cell lines. The immune-stimulating effects of U. lactuca fractions on rat macrophage cell line (RAW 264.7) were also investigated. All five fractions of U. lactuca extract stimulated NO production with concentration dependant manner. These results suggest that U. lactuca may be a useful candidate for a natural cancer preventing and immune-stimulating agents.

• Molecules and Cells
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• v.40 no.2
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• pp.100-108
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• 2017

Cathepsin F, which is encoded by CTSF, is a cysteine proteinase ubiquitously expressed in several tissues. In a previous study, novel transcripts of the CTSF gene were identified in the crab-eating monkey deriving from the integration of an Alu element-AluYRa1. The occurrence of AluYRa1-derived alternative transcripts and the mechanism of exonization events in the CTSF gene of human, rhesus monkey, and crabeating monkey were investigated using PCR and reverse transcription PCR on the genomic DNA and cDNA isolated from several tissues. Results demonstrated that AluYRa1 was only integrated into the genome of Macaca species and this lineage-specific integration led to exonization events by producing a conserved 3' splice site. Six transcript variants (V1-V6) were generated by alternative splicing (AS) events, including intron retention and alternative 5' splice sites in the 5' and 3' flanking regions of CTSF_AluYRa1. Among them, V3-V5 transcripts were ubiquitously expressed in all tissues of rhesus monkey and crab-eating monkey, whereas AluYRa1-exonized V1 was dominantly expressed in the testis of the crab-eating monkey, and V2 was only expressed in the testis of the two monkeys. These five transcript variants also had different amino acid sequences in the C-terminal region of CTSF, as compared to reference sequences. Thus, species-specific Alu-derived exonization by lineage-specific integration of Alu elements and AS events seems to have played an important role during primate evolution by producing transcript variants and gene diversification.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.37 no.4
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• pp.427-432
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• 2024

The polymer crystallization process, promoting the formation of ferroelectric β-phase, is essential for developing polyvinylidene fluoride (PVDF)-based high-performance piezoelectric energy harvesters. However, traditional high-temperature annealing is unsuitable for the manufacture of flexible piezoelectric devices due to the thermal damage to plastic components that occurs during the long processing times. In this study, we investigated the feasibility of introducing a flash lamp annealing that can rapidly induce the β-phase in the PVDF layer while avoiding device damage through selective heating. The flash light-irradiated PVDF films achieved a maximum β-phase content of 76.52% under an applied voltage of 300 V and an on-time of 1.5 ms, a higher fraction than that obtained through thermal annealing. The PVDF-based piezoelectric energy harvester with the optimized irradiation condition generates a stable output voltage of 0.23 V and a current of 102 nA under repeated bendings. These results demonstrate that flash lamp annealing can be an effective process for realizing the mass production of PVDF-based flexible electronics.

• Weed & Turfgrass Science
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• v.3 no.4
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• pp.305-311
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• 2014

The cultivation area and use of genetically modified (GM) crops have been increased continuously over the world. Seed distribution and transgenes to environmental ecosystem is one of the most important factors in risk assessment and risk management of GM crop. Safe management for the development and commercialization of GM crops is required according to The Act on Transboundary Movements of Living Modified Organisms,etc (LMO Act) in Korea. This study was conducted to setup the environmental monitoring system of GM zoysiagrass (event JG21 and JG21-MS). The monitoring was performed in 4 GMO confined fields, Sungwhan, Ochang, Jeju University and Jeju Namwon. In the result of monitoring, we could not found any gene flow and distribution of GM zoysiagrass in the 3 fields, but one spill of JG21 was found in the Namwon field in 2012. These results suggest that continuous monitoring is necessary to detect the occurrence of GM zoysiagrass for preventing genetic contamination in natural environment.

• Journal of the Korean Society of Food Science and Nutrition
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• v.35 no.5
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• pp.583-587
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• 2006

Aqueous solutions of sugar alone or in the presence of lysine were gamma irradiated at 0, 5, 10, 20 and 30 kGy at room temperature. Absorbances at 284 nm as an indicator of intermediate stage of non -enzymatic browning reaction increased with irradiation dose in both the solution of sugar or lysine alone and sugar-lysine mixed solution. Absorbances at 420 nm as indicator of browning increased in the irradiated sugar-lysine mixed solutions although no browning was observed in the irradiated solution of sugar or lysine alone. The degree of browning of the irradiated sugar-lysine mixed solution increased with irradiation dose and was dependent on the type of sugar. For sugar-lysine mixed solution irradiated at 30 kGy, the browning had the following order of intensity: sucrose>fructose>arabinose>xylose>glucose. However, the sugar loss of irradiated sugar lysine mixed solution had a following order of intensity: glucose>fructose>sucrose>xylose>arabinose. The reducing power of the non-reducing sugar, sucrose, was produced by gamma irradiation. The present results indicated that gamma irradiation leads to a non-enzymatic browning reaction (carbonyl-amine reaction) in an aqueous system.

• Journal of Korean Home Economics Education Association
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• v.19 no.1 s.43
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• pp.15-33
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• 2007

The purpose of this study is to find the effect of improvement in student's learning attitude when applying portfolio, which is one of the ways for the performance's assessment suggested as an alternative of traditional assessment for the instruction of Home Economics subject. The results of this study were as follows; 1. We have developed the Portfolio materials; teaching and learning plans, the paper of activities of the class, materials of the instructions, and assessment materials from the instruction of total 13 times in the unit of 'making short pants'. 2. The instruction,'Making short pants' with applying portfolio, is very effective in terms of changing student's learning attitude positively - pride, self-confidence, interest, conscience of purpose, motivation for achievement, focus surroundings, self-study, friendship among classmate and etc. 3. The result of analyzing students 'thoughts shows that the majority over 90% of the students showed the positive attitude toward applied portfolio instruction. Here are the reasons that 'it's helpful to study', 'it can be focused better on the instruction', 'it give them satisfaction and couidence' and 'it can be increased abilities studying by oneself'. on the other hand, the negative side of the instruction applied portfolio is that it requires too much time to prepare portfolio materials. there is pressure because of a lot of assignments to do. Based on this result, we are expecting to increase student's interest in the middle school class of Technology and Home Economic and also to achieve development of the method of teaching and learning to enhance student's learning attitude.

• Korean Journal of Veterinary Research
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• v.42 no.3
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• pp.411-418
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• 2002

Thirty rabbits underwent femoral lengthening using monolateral external fixator to evaluate results and complications of limb lengthening. Twenty rabbits survived until consolidation of callus formed at the lengthening site after finishing lengthening. Ten rabbits were sacrificed during femoral lengthening because of complications. Survived 20 rabbits were classified to two groups according the amount of lengthening: group I (10% lengthening of the femoral length); group II (20% lengthening). There was no significant difference of consolidation time between two groups (p=0.25). Varus angulation at the lengthening site occurred in 60% of two groups and the amount of varus angulation in group II was larger than that of group I. Degenerative change of articular cartilage at the medial condyle of the distal femur was found in 30% of group II. Of sacrificed ten rabbits, 5 had pin loosenings with pull an of pins from the femur, 2 had fermoral fractures around the pin-tract site, and 3 had severe osteomyelitis of the femur around the pin-tract site.

• Korean Journal of Veterinary Research
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• v.42 no.3
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• pp.403-410
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• 2002

This study was performed to investigate the general prepartum behavioral ethogram of Bos taurus coreanae (Hanwoo cow). In this study, 4 pregnant cows were placed in a separate area. We recorded the behaviors of the cows using time lapse VCR for 48 hours and analyzed behaviors with the scan point sampling method. We observed maintenance behaviors, social behaviors and ingestion behaviors. During the observation period, the time budgets of behaviors in order of frequency were LD(lying down, 38.2%), ST(standing, 24.7%), EA(eating, 10.7%), WA(walking, 7.2%), LR(lying down rumination, 5.6%), SR(standing rumination, 3.3%), TW(tail wagging, 3.1%) and SG(self grooming, 1.8%). The time budgets of the other behaviors such as PG(pairwise grooming), FC(fly catching) were negligible (<1%).