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http://dx.doi.org/10.5352/JLS.2006.16.7.1169

Effects of Ulva lactuca Extracts on Cytotoxicity of Cancer Cell Lines and Immune Stimulation  

Jang, Min-Kyung (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
Kim, Nam-Young (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
Lee, Dong-Geun (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
Lee, Jae-Hwa (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
Ha, Jong-Myung (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
Ha, Bae-Jin (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
Kim, Mi-Hyang (Department of Food and Nutrition, College of Medical Life Science, Silla University)
Bae, Song-Ja (Department of Food and Nutrition, College of Medical Life Science, Silla University)
Jang, Jeong-Su (Central Research Institute, Binex Co., Ltd.)
Lee, Sang-Hyeon (Department of Pharmaceutical Engineering, College of Medical Life Science, Silla University)
Publication Information
Journal of Life Science / v.16, no.7, 2006 , pp. 1169-1173 More about this Journal
Abstract
Extracted fractions of the green seaweed Ulva lactuca were studied to verify the cytotoxicity and immunostimulating activity. The fractions from the ethanol extract of U. lactuca were prepared by the systematic extraction procedure with solvents such as hexane, ethyl ether, methanol, butanol and $H_2O$. The cytotoxic effects of U. lactuca fractions against human leukemia cell line U937, mouse neuroblastoma cell line (NB41A3), human hepatoma cell line (HepG2) and rat glioma cell line (C6) were investigated. Ethyl ether fraction showed the highest cytotoxicity against all four cell lines tested. In addition, $H_2O$ fraction also showed relatively high cytotoxicity. Dose dependent patterns were observed on all four cell lines. The immune-stimulating effects of U. lactuca fractions on rat macrophage cell line (RAW 264.7) were also investigated. All five fractions of U. lactuca extract stimulated NO production with concentration dependant manner. These results suggest that U. lactuca may be a useful candidate for a natural cancer preventing and immune-stimulating agents.
Keywords
C6; HepG2; MTT assay; NB41A3; NO assay; RAW 264.7; U937; Ulva lactuca;
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