• Development and Reproduction
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• v.23 no.1
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• pp.35-42
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• 2019

Fish shows great difference in growth rate between individuals during larval development and early growth. This difference seriously reduces the production efficiency in fish culture. Growth hormone (GH)/Insulin-like growth factor 1 (IGF1) system is said to play some pivotal roles in fish growth. In this study, we investigated differences of GH, IGF1 and GHR gene expressions in juvenile red spotted grouper (Epinephelus akaara) with different growth performance. Red spotted groupers were reared under the same environmental condition (water temperature $24{\pm}1^{\circ}C$, natural light) for 96 days after hatching. They were divided into 3 groups by size (fast growing, middle growing and slow growing groups: FGG, MGG, and SGG, respectively). RNA was extracted from the brain, liver and muscle tissues from each group, and target gene expression was examined by real-time PCR. In the brain with pituitary gland, expression of GH gene in FGG was significantly higher than the expression in SGG, but the expression of IGF1 and GHR genes in the muscle was highest in SGG. Difference of GHR and IGF1 mRNA in the liver between groups with different growth performance was less clear than that in other tissues, although level of IGF1 mRNA was higher in SGG than in MGG. These results suggest that hormonal governing of growth is not the same in fast growing and slow growing fish, and size grading could cause a shift of hormonal state and growth pattern in this species.

• Journal of Veterinary Science
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• v.21 no.5
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• pp.74.1-74.13
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• 2020

Background: The quality of a vaccine depends strongly on the effects of the adjuvants applied simultaneously with the antigen in the vaccine. The adjuvants enhance the protective effect of the vaccine against a viral challenge. Conversely, oil-type adjuvants leave oil residue inside the bodies of the injected animals that can produce a local reaction in the muscle. The long-term immunogenicity of mice after vaccination was examined. ISA206 or ISA15 oil adjuvants maintained the best immunity, protective capability, and safety among the oil adjuvants in the experimental group. Objectives: This study screened the adjuvant composites aimed at enhancing foot-and-mouth disease (FMD) immunity. The C-type lectin or toll-like receptor (TLR) agonist showed the most improved protection rate. Methods: Experimental vaccines were fabricated by mixing various known oil adjuvants and composites that can act as immunogenic adjuvants (gel, saponin, and other components) and examined the enhancement effect on the vaccine. Results: The water in oil (W/O) and water in oil in water (W/O/W) adjuvants showed better immune effects than the oil in water (O/W) adjuvants, which have a small volume of oil component. The W/O type left the largest amount of oil residue, followed by W/O/W and O/W types. In the mouse model, intramuscular inoculation showed a better protection rate than subcutaneous inoculation. Moreover, the protective effect was particularly weak in the case of inoculation in fatty tissue. The initial immune reaction and persistence of long-term immunity were also confirmed in an immune reaction on pigs. Conclusions: The new experimental vaccine with immunostimulants produces improved immune responses and safety in pigs than general oil-adjuvanted vaccines.

• Research in Community and Public Health Nursing
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• v.10 no.2
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• pp.275-290
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• 1999

The Objectives of this study were to identify family nursing phenomena at the community in Korea and to contribute to build up family domain of International Classification for Nursing Practice. The method of this study was used retrospective one among three methods to develop ICNP during the period from April 1997 to June 1999. The procedure was to choose nursing phenomena using preliminary terms(stepl) from the reports on family nursing care of the nursing students of 5 junior colleges of nursing and 5 colleges of nursing. The study group members identified 3 common family nursing phenomena with 5 characteristics related to each phenomenon. In order to consensus the appropriate characteristics of a phenomenon(step2), 17 study group members had regrouped nursing phenomena and scored its characteristics 5 times. The essential characteristics of each family phenomenon were selected above 3.5 mean score from related characteristics(step 3). Finally, 17 phenomena were named preferred terms such as following, that was selected after investigated preliminary terms(step4). Family nursing phenomena in Korea are named as Lack of family interaction in community. Social isolation. Lack of social support system in community. Disturbance in parent role, Disturbance in marital role, Dissatisfaction of sexual life, Disturbance in family communication, Inappropriate family coping, Lack of family intimacy, Inappropriate family power structure, Family violence. Unhealthy life style. Deficit of financial management skill and support. Inadequate care a sick member. Insecure safety and hygiene in neighborhood, Inadequate home-sanitation. Inadequate home-making. Family nursing phenomena in Korea were partially confirmed family architecture of ICNP, Beta version. by this study. Further study on Family nursing phenomena in Korea will be required to support evidence through literature review of nursing classifications or field studies.

• Quality Improvement in Health Care
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• v.8 no.1
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• pp.44-55
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• 2001

Background : This study aimed at identifying the effect of the standardized protocol on lowering the incidence of the ventilator associated pneumonia(VAP). Methods : The standard protocol focusing on decreasing VAP was made and applied at 5 ICUs (Medical ICU, surgical ICU, Respiratory ICU, Neonatal ICU, Pediatric ICU) in a university affiliated tertiary hospital, from April 1, 2000 to Oct 31, 2000. The protocol involved 3 parts : hand washing, the suctioning method and ventilator circuit management. All the nursing personnel received intensive education which was consisted of lecture, video film and demonstration. 176 nurses reported the performance of handwashing pre and post intervention. And randomly selected 15 nurses were observed by charge nurse and the handwashing practice was analyzed pre and post intervention. The incidence of VAP was compared with the former year incidence. Results : The self reported frequency of hand washing increased. In the direct observation of handwashing, the frequency, time, thoroughness of hand washing during 8 hours day duty was found to be improved. The frequency was increased from 1.1 time to 4.1 times; the time was improved from 1.7 seconds to 5.7 seconds and the thoroughness of the washing practice was from 0.2 times to 3.0 times respectively (p<0.001). The incidence of VAP decreased from at a rate of 15.63 number of case per 1,000 ventilator-day (April 1~August 31, 1999) to 7.23 number of case per 1,000 ventilator-days(April 1~Oct 31, 2000)(P<0.001). Conclusion : We developed the protocols which included hand washing, the suctioning method, and ventilator circuit management. Through the implementation of the protocol, the performance of hand washing improved and the VAP incidence rate in ICU was decreased.

• Journal of Korean Academy of Nursing Administration
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• v.12 no.3
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• pp.424-433
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• 2006

Purpose: This study is analyzing nurse managers's common fundamental competencies changes and responses to the application of the action learning, and promoting introduction and revitalization of action learning. Method: The research design is one group pretest-posttest design. The subjects are 40 nurse managers. The program was performed for 5 weeks. Data were collected before the program and after 5 weeks program and were analyzed with paired t-test. Result: Action learning was an effective way to develop an individual and team and to solve the problem of organization. There were more significant increases after the program than before the program on common fundamental competencies(p<.05). Conclusion: This study has us confirm that action learning is an effective education method for organization members on the spot to recognize their problem and study by themselves and solve the practical problems with various solution process.

• Journal of Korean Academy of Fundamentals of Nursing
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• v.18 no.2
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• pp.267-276
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• 2011

Purpose: The purpose of this study was to provide data for development of the Journal of Fundamentals of Nursing through reviewing classification of sectional editorship. Method: Descriptive statistical analysis was used, to identify content then, the classifications of sectional editorship were reviewed. Results: The categories, "Basic concepts of nursing" (30.2%:17.9%), "Needs for comfort" (15.9%:13.1%), "Nursing education" (12.7%:12.7%), and "Needs for activity and exercise" (7.1%:7.1%) were most frequent through both the 1st and 2nd sectional editorship. Conclusion: Classification of sectional editorship was developed to clarify the educational purpose of fundamentals of nursing, and was modified through reflections by researchers. The current classification of sectional editorship is not a permanent formula, so using various viewpoints in further examination is needed.

• Journal of Korean Academy of Nursing Administration
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• v.13 no.1
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• pp.53-64
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• 2007

Purpose: This study was aimed to develop and evaluate of a Problem-based Learning (PBL) in the course of Nursing Management and Ethics. Method: The design of the study was both methodological and one group only pre-post design. The sample included 61 senior students who are currently enrolled in Nursing management and Ethics course in college of nursing. Data regarding PBL evaluation were collected on the critical thinking and clinical reasoning using structured questionnaires during March to June, 2005. Data were analyzed using descriptives and paired t-test. Results: A total of three PBL packages was developed by the two faculty members and two teaching assistants who are majoring in nursing management. PBL packages that had been developed was applied to 61 senior students for three months. Critical thinking and clinical reasoning were measured twice pre and post the application of PBL packages. There were statistically significant differences in the critical thinking and clinical reasoning between the pre and post PBL application. Conclusion: PBL was considered to be effective in understanding the learning concepts in the Nursing Management and Ethics. Further research on the facilitative strategies and development model considering the characteristics of Nursing Management and Ethics course is needed.

• Journal of Korean Academy of Nursing Administration
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• v.12 no.1
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• pp.113-121
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• 2006

Purpose: This study was aimed to investigate educational need of nursing managerial competency in the hospitals. Method: The data were collected from 296 nurses(nurse manager and nurse) who had worked in 9 hospitals using the structured questionnaires. Data was analysed by the SPSS for Windows 12.0 program. Results: Educational needs of the nurse managerial competency development were very high. Most of items in the areas of 'human resource management', 'leadership', 'ethic/ law responsibility' and 'quality improvement' were needed for nurse managerial competency development. Current level of the nurse managerial competency level in practice was lower than education need. However nurses had moderate level ability in the areas of 'ethic/ law responsibility', 'human resource management' and 'nursing information management'. There were significant differences in current level and need of the nurse managerial competency(p=.000). Education needs of the nurse managerial competency were influenced by age, clinical career, position, education level, experience of nurse manager education(p<.05). 5) Education program operations were preferred to 'the theme course', 'lecture', 'external education institution'. Conclusion: Education program of the nursing managerial competency development is urgently needed for nurses who have worked in a hospital.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.21 no.6
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• pp.583-590
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• 2010

In this paper, a novel CRLH-TL unit cell with controllable transmission zeros was proposed. Proposed composite right/left handed transmission line(CRLH-TL) unit cell is implemented in the form of the metal-insulator-metal(MIM) capacitors, the microstrip stub inductors, and the co-planar waveguide(CPW) inductor. And this proposed CRLH-TL generates two transmission zeros in lower/upper passband by the effort of electromagnetic couplings between each MIM capacitors and microstrip stub inductors. Using this proposed CRLH-TL, broad bandpass filter for UWB system was designed and fabricated. The measured results reveal that the two transmission zeros are observed in lower/upper passband and the overall size of the filter, excluding the feed line is about 8 mm$\times$8 mm, less then $\lambda_g$/4 on electric size.

• Journal of Physiology & Pathology in Korean Medicine
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• v.22 no.4
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• pp.771-777
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• 2008

Chungpaesagan-tang which is used for treating patients of brain in cerebrovascular disease frequently from clinical doctor has not reported about the effect of neuronal aptosis caused of brain ischemia. The aim of this study is to investigate effect of Chungpaesagan-tang protecting neuronal cells from being damaged by brain ischemia through using organotypic hippocampal slice cultures. We caused ischemic damage to organotypic hippocampal slice cultures by oxygen and glucose deprivation. And added Chungpaesagan-tang extract to cultures. thereafter we measured area percentage of propidium iodide (PI)-stained neuronal cell, lactate dehydrogenase (LDH) levels in culture media and Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL)-positive cells. Area percentage of PI-stained neuronal cells and count of TUNEL-positive cells in CA1 and DG area of organotypic hippocampal slice culture were significantly decreased in pertinent density level of Chungpaesagan-tang extract. LDH levels in culture media of organotypic hippocampal slice culture were significantly decreased in pertinent density level of Chungpaesagan-tang extract. Within pertinent density level, Chungpaesagan-tang has cell protection effect that prevents brain ischemia damaging neuronal cells and apoptosis increasing.