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Effect of Chungpaesagan-tang on Ischemic Damage in Organotypic Hippocampal Slice Culture  

Lee, Min-Young (College of Oriental Medicine, Institute of Oriental Medicine)
Ku, Ja-Seung (Graduate School of East-West Medicine, Kyung Hee University)
Kim, Sung-Hoon (Graduate School of East-West Medicine, Kyung Hee University)
Kim, Yoon-Bum (College of Oriental Medicine, Institute of Oriental Medicine)
Kim, Sun-Yeou (Graduate School of East-West Medicine, Kyung Hee University)
Choi, Hyeon (Hamsoa Pharmaceutical Co.)
Sohn, Young-Joo (College of Oriental Medicine, Sangji University)
Jung, Hyuk-Sang (College of Oriental Medicine, Institute of Oriental Medicine)
Sohn, Nak-Won (Graduate School of East-West Medicine, Kyung Hee University)
Publication Information
Journal of Physiology & Pathology in Korean Medicine / v.22, no.4, 2008 , pp. 771-777 More about this Journal
Abstract
Chungpaesagan-tang which is used for treating patients of brain in cerebrovascular disease frequently from clinical doctor has not reported about the effect of neuronal aptosis caused of brain ischemia. The aim of this study is to investigate effect of Chungpaesagan-tang protecting neuronal cells from being damaged by brain ischemia through using organotypic hippocampal slice cultures. We caused ischemic damage to organotypic hippocampal slice cultures by oxygen and glucose deprivation. And added Chungpaesagan-tang extract to cultures. thereafter we measured area percentage of propidium iodide (PI)-stained neuronal cell, lactate dehydrogenase (LDH) levels in culture media and Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL)-positive cells. Area percentage of PI-stained neuronal cells and count of TUNEL-positive cells in CA1 and DG area of organotypic hippocampal slice culture were significantly decreased in pertinent density level of Chungpaesagan-tang extract. LDH levels in culture media of organotypic hippocampal slice culture were significantly decreased in pertinent density level of Chungpaesagan-tang extract. Within pertinent density level, Chungpaesagan-tang has cell protection effect that prevents brain ischemia damaging neuronal cells and apoptosis increasing.
Keywords
organotypic hippocampal slice culture; propidium iodide (PI); lactate dehydrogenase (LDH); TUNEL; Chungpaesagan-tang;
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