Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effect of Chungpaesagan-tang on Ischemic Damage in Organotypic Hippocampal Slice Culture

청폐사간탕(淸肺瀉肝湯)이 뇌해마 조직배양의 신경세포 자연사에 미치는 영향

  • Lee, Min-Young (College of Oriental Medicine, Institute of Oriental Medicine) ;
  • Ku, Ja-Seung (Graduate School of East-West Medicine, Kyung Hee University) ;
  • Kim, Sung-Hoon (Graduate School of East-West Medicine, Kyung Hee University) ;
  • Kim, Yoon-Bum (College of Oriental Medicine, Institute of Oriental Medicine) ;
  • Kim, Sun-Yeou (Graduate School of East-West Medicine, Kyung Hee University) ;
  • Choi, Hyeon (Hamsoa Pharmaceutical Co.) ;
  • Sohn, Young-Joo (College of Oriental Medicine, Sangji University) ;
  • Jung, Hyuk-Sang (College of Oriental Medicine, Institute of Oriental Medicine) ;
  • Sohn, Nak-Won (Graduate School of East-West Medicine, Kyung Hee University)
  • 이민영 (경희대학교 한의과대학 한의학연구소) ;
  • 구자승 (경희대학교 동서의학대학원) ;
  • 김성훈 (경희대학교 동서의학대학원) ;
  • 김윤범 (경희대학교 한의과대학 한의학연구소) ;
  • 김선여 (경희대학교 동서의학대학원) ;
  • 최현 ((주)함소아 제약) ;
  • 손영주 (상지대학교 한의과대학) ;
  • 정혁상 (경희대학교 한의과대학 한의학연구소) ;
  • 손낙원 (경희대학교 동서의학대학원)
  • Published : 2008.08.25
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Abstract

Chungpaesagan-tang which is used for treating patients of brain in cerebrovascular disease frequently from clinical doctor has not reported about the effect of neuronal aptosis caused of brain ischemia. The aim of this study is to investigate effect of Chungpaesagan-tang protecting neuronal cells from being damaged by brain ischemia through using organotypic hippocampal slice cultures. We caused ischemic damage to organotypic hippocampal slice cultures by oxygen and glucose deprivation. And added Chungpaesagan-tang extract to cultures. thereafter we measured area percentage of propidium iodide (PI)-stained neuronal cell, lactate dehydrogenase (LDH) levels in culture media and Terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL)-positive cells. Area percentage of PI-stained neuronal cells and count of TUNEL-positive cells in CA1 and DG area of organotypic hippocampal slice culture were significantly decreased in pertinent density level of Chungpaesagan-tang extract. LDH levels in culture media of organotypic hippocampal slice culture were significantly decreased in pertinent density level of Chungpaesagan-tang extract. Within pertinent density level, Chungpaesagan-tang has cell protection effect that prevents brain ischemia damaging neuronal cells and apoptosis increasing.

Keywords

References

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