• YAKHAK HOEJI
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• v.41 no.6
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• pp.749-755
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• 1997

It has been reported that dose-dependent $Ca^{2+}$ increase by menadione in platelets could be measured by fluorescent dye, quin-2. The problems will be described here rel ating to measuring $Ca^{2+}$ in menadione-exposed platelets using fura-2 and fluo-3, widely used fluorescent indicators. Additions of menadione to fura-2 loaded platelets and their lysates resulted in marked reduction in fluorescence intensity at both 340nm ($Ca^{2+}$-unbound form) 380nm ($Ca^{2+}$-undbound form) excitation wavelengths. Fura-2 excitation spectra were overlapped with UV-visible absorption spectra of menadione, suggesting that light absorption by menadione itself could quench fluorescence generated by fura-2. Next approach was to use fluo-3 which has the higher wavelength (490nm) of excitation. Previous work demonstrated that treatment with probenecid to platelets was required to prevent fluo-3 dye leakage. However, probenecid itself was proven to be inadequate to measure the concentration of intracellular $Ca^{2+}$; by reducing menadione-induced cytotoxicity in platelets. Our results suggest that it is not feasible to measure $Ca^{2+}$ in platelets by using fura-2 and fluo-3 in the presence of probenecid, and cautions should be taken to measure changes of intracellular $Ca^{2+}$ levels by fluorescent dyes following chemical exposure.

• Journal of the Institute of Electronics Engineers of Korea SC
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• v.41 no.6
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• pp.17-25
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• 2004

In this paper, we propose a new small swing domino logic for low-power consumption. To reduce the power consumption, both the precharge node and the output node swing the range from 0 to $V_{REF}$- $V_{THN}$, where $V_{REF}$=VDD-n $V_{THN}$ (n=1, 2, and 3). This can be done by adding the inverter structure on domino logic that allows a full swing or a small swing on its input terminal without leakage current. Compared to previous works, the proposed structure can save the power consumption of more than 30% for n=0, 1, 2, and 3 in the equation of $V_{REF}$=VDD-n $V_{THN}$. A multiplier applying the proposed domino logic has been designed and fabricated using a 0.35-${\mu}{\textrm}{m}$ n-well CMOS process under 3.3-V supply voltage. Compared with other previous works, it shows a 30% power reduction and a better feature in power-delay product.lay product.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.48 no.2
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• pp.20-27
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• 2011

A CMOS Image Sensor(CIS) mounted on mobile appliances always needs a low power consumption because of the battery life cycle. In this paper, we propose novel power reduction techniques such as a data flip-flop circuit with leakage current elimination, a low power single slope A/D converter with a novel comparator, and etc. Based on 0.13um CMOS process, the chip satisfies QVGA resolution($320{\times}240$ pixels) whose pitch is 2.25um and whose structure is 4-Tr active pixel sensor. From the experimental results, the ADC in the middle of CIS has a 10-b resolution, the operating speed of CIS is 16 frame/s, and the power dissipation is 25mW at 3.3V(Analog)/1.8V(Digital) power supply. When we compare the proposed CIS with conventional ones, the power consumption is reduced approximately by 22% in sleep mode, 20% in operating mode.

• Journal of the Korean Institute of Gas
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• v.19 no.4
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• pp.49-54
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• 2015

The purpose of this study is to assess the risk of depending on the presence or absence of safety device of domestic heating and drying furnaces, by derivation and analysis of accident frequency of safety devices through FTA (Fault Tree Analysis). Installation standards are lacking in Korean for the safety device of LPG heating and drying furnace, which have a risk of explosion due to structure to trap the leaked gas. Four different safety devices were selected on the basis of NFPA and national standards for combustors of other equipment. Effects of frequency reduction in accidents were analyzed before and after installing the safety devices respectively. As a result, a minimal leakage safety device was presented for preventing damages from gas leak of domestic LPG heating and drying furnace.

• The Transactions of the Korean Institute of Power Electronics
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• v.22 no.6
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• pp.551-554
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• 2017

This paper proposes a three-level LLC resonant converter using integrated magnetics (IM). Given that the switch voltage stress of the proposed converter is guaranteed to be half of the input voltage, the switching losses can be greatly reduced, thereby benefitting the high-frequency operation. To reduce the volume of reactive components such as transformers, high-frequency driving and planar core are applied. However, two resonant inductors and one transformer are required because of the three-level structure and the limited leakage inductance of the planar transformer for the resonant operation. Therefore, the effect of volume reduction is not very large. In order to solve these drawbacks, this paper proposes a new IM that integrates all magnetic elements used in the proposed three-level resonant converter by using the magnetizing inductor as a resonant inductor. The experimental results are presented by conducting a theoretical analysis of a prototype with 350 W to 800 kHz.

• Journal of the Korean Professional Engineers Association
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• v.1 no.1
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• pp.18-25
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• 1968

Higher yield in rice paddies is greatly dependent on adequately balanced and timely supply of water. A majority of rice paddy in Korea is generally irrigated by rainfall, but in many cases it has to be supplemented by artificial irrigation for optimum rice culture. Although the water requirement of rice plant is for higher Than that of athec crops, submerged condition of rice paddy is not Necessarily required. The moisture requirement of rice plant varies with its growing stages, and it is possible to increase the irrigation efficiency through reduction of water loss due to percolation in rice paddies. An experimental plots were set up by means of sandomized block design with three duplication; (a) All time submerged, (b) Economically controlled, and (c) Extremely controlled. Three different irrigation periods Were (a) Initial sage, (b) Inter-stage, and (c) Yast stage. The topsoil of the three plots were excavated to the depth of 30 cm and then compacted with clay of 6 cm thickness. There after, they were piled up with the excavated top soils, leveled and cored with clay of 6 cm thickness around footpath in order to prevent leakage. The results obtained from the experiments are as follows, 1. There is no difference among the three experiments plots in terms of physical and chemical conditions, soil properties, and other characteristics. 2. Culm length and ear length are not affected by different irrigation methods. 3. There is no difference in the mature rate and 1, 000 grain weight of rice for the three plots. 4. The control plot which was irrigated every three days shows an increased yield over the all the time submerged plot by 17.8 percent. 5. The clay lined plot whose water holding capacity was held 5 days long, needs only to be irrigated every 7 days. 6. The clay lined plot shows an increased yield over the untreated plot ; over all-the-time submerged plot by 18 percent ; extremely controlled plot by 18 percent, and economically controlled plot by 33 percent. 7. It may be saved in water requirement about one Thirds.

• Tribology and Lubricants
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• v.35 no.5
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• pp.267-273
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• 2019

Wheel bearings are important automotive parts that bear the vehicle weight and translate rotation motion; in addition, their seals are components that prevent grease leakage and foreign material from entering from the outside of the bearings. Recently, as the need for electric vehicles and eco-friendly vehicles has been emerging, the reduction in fuel consumption and $CO_2$ emissions are becoming the most important issues for automobile manufacturers. In the case of wheel bearings, seals are a key part of drag torque. In this study, we investigate the prediction of the drag torque taking into consideration the hyperelastic and viscoelastic material properties of automotive wheel bearing seals. Numerical analysis based on the finite element method is conducted for the deformation analyses of the seals. To improve the reliability of the rubber seal analysis, three types of rubber material properties are considered, and analysis is conducted using the hyperelastic material properties. Viscoelastic material property tests are also conducted. Deformation analysis considering the hyperelastic and viscoelastic material properties is performed, and the effects of the viscoelastic material properties are compared with the results obtained by the consideration of the hyperelastic material properties. As a result of these analyses, the drag torque is 0.29 Nm when the hyperelastic characteristics are taken into account, and the drag torque is 0.27 Nm when both the hyperelastic and viscoelastic characteristics are taken into account. Therefore, it is determined that the analysis considering both hyperelastic and viscoelastic characteristics must be performed because of its reliability in predicting the drag torque of the rubber seals.

• Smart Structures and Systems
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• v.6 no.5_6
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• pp.675-687
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• 2010

Structural Health Monitoring (SHM) is the science and technology of monitoring and assessing the condition of aerospace, civil and mechanical infrastructures using a sensing system integrated into the structure. Impedance-based SHM measures impedance of a structure using a PZT (Lead Zirconate Titanate) patch. This paper presents a low-power wireless autonomous and active SHM node called Autonomous SHM Sensor 2 (ASN-2), which is based on the impedance method. In this study, we incorporated three methods to save power. First, entire data processing is performed on-board, which minimizes radio transmission time. Considering that the radio of a wireless sensor node consumes the highest power among all modules, reduction of the transmission time saves substantial power. Second, a rectangular pulse train is used to excite a PZT patch instead of a sinusoidal wave. This eliminates a digital-to-analog converter and reduces the memory space. Third, ASN-2 senses the phase of the response signal instead of the magnitude. Sensing the phase of the signal eliminates an analog-to-digital converter and Fast Fourier Transform operation, which not only saves power, but also enables us to use a low-end low-power processor. Our SHM sensor node ASN-2 is implemented using a TI MSP430 microcontroller evaluation board. A cluster of ASN-2 nodes forms a wireless network. Each node wakes up at a predetermined interval, such as once in four hours, performs an SHM operation, reports the result to the central node wirelessly, and returns to sleep. The power consumption of our ASN-2 is 0.15 mW during the inactive mode and 18 mW during the active mode. Each SHM operation takes about 13 seconds to consume 236 mJ. When our ASN-2 operates once in every four hours, it is estimated to run for about 2.5 years with two AAA-size batteries ignoring the internal battery leakage.

• The Plant Pathology Journal
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• v.37 no.5
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• pp.437-445
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• 2021

Tilletia laevis Kuhn (syn. Tilletia foetida (Wallr.) Liro.) causes wheat common bunt, which is one of the most devastating plant diseases in the world. Common bunt can result in a reduction of 80% or even a total loss of wheat production. In this study, the characteristics of T. laevis infection in compatible wheat plants were defined based on the combination of scanning electron microscopy, transmission electron microscopy and laser scanning confocal microscopy. We found T. laevis could lead to the abnormal growth of wheat tissues and cells, such as leakage of chloroplasts, deformities, disordered arrangements of mesophyll cells and also thickening of the cell wall of mesophyll cells in leaf tissue. What's more, T. laevis teliospores were found in the roots, stems, flag leaves, and glumes of infected wheat plants instead of just in the ovaries, as previously reported. The abnormal characteristics caused by T. laevis may be used for early detection of this pathogen instead of molecular markers in addition to providing theoretical insights into T. laevis and wheat interactions for breeding of common bunt resistance.

• Journal of Microbiology and Biotechnology
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• v.29 no.7
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• pp.1124-1136
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• 2019

Salinity is one of the major abiotic stresses that cause reduction of plant growth and crop productivity. It has been reported that plant growth-promoting bacteria (PGPB) could confer abiotic stress tolerance to plants. In a previous study, we screened bacterial strains capable of enhancing plant health under abiotic stresses and identified these strains based on 16s rRNA sequencing analysis. In this study, we investigated the effects of two selected strains, Bacillus aryabhattai H19-1 and B. mesonae H20-5, on responses of tomato plants against salinity stress. As a result, they alleviated decrease in plant growth and chlorophyll content; only strain H19-1 increased carotenoid content compared to that in untreated plants under salinity stress. Strains H19-1 and H20-5 significantly decreased electrolyte leakage, whereas they increased $Ca^{2+}$ content compared to that in the untreated control. Our results also indicated that H20-5-treated plants accumulated significantly higher levels of proline, abscisic acid (ABA), and antioxidant enzyme activities compared to untreated and H19-1-treated plants during salinity stress. Moreover, strain H20-5 upregulated 9-cisepoxycarotenoid dioxygenase 1 (NCED1) and abscisic acid-response element-binding proteins 1 (AREB1) genes, otherwise strain H19-1 downregulated AREB1 in tomato plants after the salinity challenge. These findings demonstrated that strains H19-1 and H20-5 induced ABA-independent and -dependent salinity tolerance, respectively, in tomato plants, therefore these strains can be used as effective bio-fertilizers for sustainable agriculture.