• Journal of Bio-Environment Control
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• v.13 no.1
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• pp.21-25
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• 2004

This experiment was carried out to investigate the effect of apex removal at different leaf ages on the growth and yield of broccoli in pinching cultivation. Apex was pinched when 2, 3 or 4 true leaves were spread during nursery bed, and non-pinching was conventional method. The plant height was not different among treatments but the fresh weight was decreased as the pinching days were delayed. The numbers of leaves and lateral shoots were increased compared to those of non-pinching by pinching. Days to central budding were shortended by 3 days when pinched at 2 true leaves spreading stage than those of non-pinching. Central (1st) head weight was heaviest when pinched at the time of 2 true leaves spreading (157g). Total head weight was heaviest when pinched at the time of 2 true leaves spreading (378g), and lowest at 4 true leaves spreading stage (196g). Marketable yields were increased by 48% when pinched at the time of 2 true leaves spreading (1,754kg) as compared with those of non-pinching (1,184kg) and lowest when pinched at the time of 4 true leaves spreading (820kg). Also, first harvest was shortended by 6 days by pinching at 2 true leaves spreading stage.

• Journal of Bio-Environment Control
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• v.14 no.4
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• pp.289-297
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• 2005

This study was conducted to develop the suitable nutrient solutions for variable Cruciferae leafy vegetables. l/2, 1 and 3/2 strength of nutrient solution recommended by National Horticultural Research Institute were supplied to plants in deep flow technique systems during 25 days. The growth of pak-choi and leaf mustard 'Asia curled' was highest in the 3/2 strength, and kaie 'TBC' in the 1 strength. Mean cation ratio of nutrient solution for pak-choi, leaf mustard and kale was K $49.5\%$, Ca $35.8\%$ and Mg $14.7\%$, which was obtained by calculating the uptake rates of water and nutrients. Suitable composition of the nutrient solution for Cruciferae leafy vegetables was N 14, P 3, K 6.8, Ca 4.8, $Mg 2m{\cdot}L^{-1}$. To examine the suitability of nutrient solution developed for Cruciferae vegetables (NSC), plants were grown 4 times from Sep. 2003 to Oct. 2004. When plants were grown in NSC, relative growth rate increased 1.1 to 2.5 times and vitamin C content 1.06 to 1.52 times. The proper plants to apply NSC for functional leaf vegetable production were leaf mustard 'Asia recurled', 'Redcurled' and 'Pamagreen', kale 'TBC', 'Portugal' and 'Hanchu collard', leaf broccoli 'New green', pak-choi, baby cabbage 'Red king' and 'Green king', flowering red chinese cabbage and Korean cabbage.

• Proceedings of the Korean Society of Food Science and Nutrition Conference
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• 2004.11a
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• pp.224-231
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• 2004

Vitamin U levels in 26 kinds of food plants are well known to healthy vegetables in Asian or Western countries were determined. Spinach showed the highest level of 452.04 mg/kg and in order Pack-choi (343.18 mg/kg) > Kale (234.18 mg/kg) > Somssukbujaengi (197.66 mg/kg) > leaf mustard (196.21 mg/kg) > aralia bud (192.50 mg/kg)> broccoli (189.03 mg/kg) >Asparagus (187.35 mg/kg). Among Korean wild medicinal plants, Sumssukbujaengi showed the highest value followed by Sanmanul (a kind of wild garlic) level of 143.46 mg/kg. For Chinese cabbages and cabbages, vitamin U showed different levels according to the parts of plant such as core, middle, outward leaves. In both samples, middle parts of leaves including midribs contained the highest level of vitamin U. The level of vitamin U was dependent on the part of the plant sample and cultivars. Leaf parts of turnip and white radish showed higher value of 84.82 mg/kg and 124.62 mg/kg than those of roots which were in order of middle (112.39 mg/kg), top (84.84 mg/kg) and bottom (84.61 mg/kg) portions in the white radish. In the analysis of amino acids, we didn't find either distinctive relationship between methionine and vitamin U synthesis or significant connection various free amino acids and vitamin U level in food plants.

• Journal of the Korean Society of Food Science and Nutrition
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• v.21 no.2
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• pp.149-153
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• 1992

The antimutagenic effect of green-ye1low vegetables on the mutagenicities induced by N-methyl-N'-nitro-N-nitrosoguanidine(MNNG) and N-nitrosodimethylamine (NDMA) in Salmonella assay system and also their inhibitory effect on AZ-521 human gastric cancer cells were studied. Twenty-four items from twenty six kinds of vegetables(92%) revealed antimutagenic activity toward MNNG (p< 0.0l, 0.05). Perilla leaf, Korean cabbage, cauliflower, lettuce, mustard leaf, water dropwort, small water dropwort, carrot and burdock inhibited the mutagenicity more than 80%. The methanol extracts of the vegetables also showed the antimutagenic activity toward NDMA (p< 0.01, 0.05). Especially, perilla leaf, kale, soybean sprout and onion inhibited more than 80% of the NDMA induced mutagenicity in S. typhimurium TA100. Small water dropwort and perilla leaf exhibited the strong inhibitory effect (97~100%) on the growth of the AZ-521 human gastric cancer cells. Soybean sprout, water dropwort, broccoli, crown daisy, green red pepper, red pepper leaves, spinach, cabbage and sweet potato also inhibited growth of the cancer cells (p < 0.001~0.05).

• Korean Journal of Plant Resources
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• v.15 no.1
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• pp.50-56
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• 2002

This study was carried out to optimize the fertigation method using fermented swine liquid manure for the growth of two western vegetables, broccoli and celery. Plants were grown in a rain-shelter house and fertilized with a range of dilutions(efflux 5 dilution=Ef. 5, efflux 10 dilution=Ef. 10, efflux 25 dilution=Ef. 25, and efflux 50 dilution=Ef. 50) of the liquid manure or with conventional application of N : P$_2$O$\_$5/ : K$_2$O = 200 : 70 : 500kg/ha for broccoli, 250 : 210 : 240 kg/ha for celery as controls. After harvest, soil pH and K content decreased after using a high concentration of the liquid manure, Ef. 5, than after treatment with weaker concentrations at Ef. 25 and Ef. 50. On the other hand, soil electrical conductivity, content of P$_2$O$\_$5/, organic matter, total nitrogen, and NO$_3$-N at Ef. 5 increased as concentration of swine liquid manure increased. After harvest, available P$_2$O$\_$5/ in plant tissue did not differ significantly between any of the treatments. In broccoli, the lower concentration (Ef. 50) of swine liquid manure increased flowering over the other treatments, perhaps because the level of absorption into the plants is higher with lower concentration. The amounts of K and Ca in plant tissue were greatest after Ef. 25 and Ef. 50 treatments. Plant growth was best at Ef. 50 in broccoli, head height, head width, and head weight were the best with Ef. 25 and Ef. 50 treatments after harvest. In celery, leaf length was greater after Ef. 25 and Ef. 50 treatments than any other treatments. Total yield of celery of Ef. 25 and Ef. 50 treatments was twice that of conventional cultivation. On the other hand, yield severely decreased after application of high-concentration treatment at Ef. 5. In conclusion, fertigation of swine liquid manure, diluted in the range of Ef. 25 to Ef. 50, could improve yield and quality in broccoli and celery.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2010.05a
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• pp.18-18
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• 2010

Glucosinolates(GSLs) are major secondary products($\beta$-thioglucoside N-hydroxysulfates) containing sulfates that are found mainly in Cruciferae family such as cabbage, broccoli, radish, turnip, kale, mustard and rapeseed as the important crops in agronomic and economic aspects. Especially, isothiocyanates(ITCs) have been shown to inhibit carcinogenesis with regulation cancer cell development followed by regulating target enzymes, inducing cell cycle arrest and apoptosis against various human cancer cell lines. Total desulfo(DS)-GSLs of nine type in 210 Korean leaf mustard accessions were isolated and confirmed using electrospray ionization mass spectrometric(ESI-MS/MS) method and DS-GSLs powerful library maked from several reference data. The isolated individual DS-GSLs were identified by removed $\beta$-D-glucopyranose residue ($C_6H_{11}O_5$, MW 163) from MS/MS data. Among them sinigrin(41.7%), glucoiberverin(21.7%) and gluconasturtiin(12.6%) were major components, Especially, despite gluconapin was minor component, accession K046197-1 and K046197-2 showed higher content of 4.11 and 3.31 mg/g(DW), respectively. The total GSLs contents in 210 accessions were ranged from 5.3 to 23.2 mg/g(DW) with a mean value of 13.0 mg/g(DW). As a result of principal component analysis(PCA), the individual GSLs loading plots were composed of three groups and components belonged to each group showed correlationship in quantitative pattern.

• The Korean Journal of Pesticide Science
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• v.17 no.4
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• pp.293-301
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• 2013

This study was carried out to investigate the pesticide residue pattern among different leafy vegetables applied with foliar spraying under greenhouse and to check extrapolating from some residue trial data to other minor crops. Leafy vegetables used in this study were: Mustard greens (Brassica juncea L.), Kale (Brassica oleracea L.), Dacheongchae (a kind of pak-choi (Brassica rapa subsp. chinensis L.)), Leaf broccoli (Brassica oleracea var alboglabra), Perilla leaf (Perilla frutescens (L.) Britton var. Frutescens), Leaf lettuce (Lactuca sativa L.), Swiss chard (Beta vulgaris L. subsp. vulgaris) and Red leaf chicory (Cichorium intybus L. var. foliosum Hegi). These are cultivated all year under indoor or outdoor and cut the leaf from plant continuously during harvest time. The amounts of pesticide deposit in/on the continuous harvesting leafy vegetables were affected by the ratios of leaf area to weight. Ratio of perilla leaf was the largest among crops as 58 $cm^2/g$. The residue levels of 7 pesticides in/on perilla leaf were the highest than those of other crops through the statistical analysis from zero day to fifth day after last application. The representative crop in 8 crops was perilla leaf selected based on the amounts of daily consumption and the high residues. This study suggest that the continuous harvesting leafy greens should be separated from the one time harvesting leafy vegetables for the pesticide recommendations because of different harvesting habits and pre-harvest intervals.

• Journal of Food Hygiene and Safety
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• v.31 no.4
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• pp.250-257
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• 2016

This study was conducted to extract the factors affecting the microbial safety of leaf and stem vegetables in the high school foodservice and to provide information for supplying the safe foodservice menu. The lunch and dinner menu (1,945 data) of the total 6 high schools at the Central and the South Region in March, June, September, and December were collected. The frequency analysis and the multiple correspondence analysis (MCA) based on the 3 factors (potentially hazardous food (PHF), leafy and stem vegetables in the menu, the cooking methods) were conducted. The most frequent PHF was the menu of blanched vegetables, salads, seaweeds and fried chicken. The most frequent consumed leaf and stem vegetables were spinach, chive, lettuce, Western cabbage, perilla leaf, iceberg lettuce, chicory, leek and broccoli. MCA based on the leaf and stem vegetables, the region, and the cooking method (cooked/non-cooked) showed that garlic stem and spinach were more used in the Central Region, while water drop-wort were more used in the South Region. Iceberg lettuce, Bok choy and leek were included frequently in the PHF menu. Plant products frequently used in PHF menu requires the food safety system such as Good Agricultural Practice (GAP) to reduce the microbial risk. The menu database according to raw materials based on cooking methods (heating or mixing) as well as the development and verification of menu based on the microbial safety will be contributed to provide the safer foodservice menu.

• The Korean Journal of Pesticide Science
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• v.18 no.4
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• pp.364-375
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• 2014

This study was carried out to investigate the pesticide efficacy and crop safety among different leafy vegetables applied with foliar spraying under greenhouse and to check extrapolating from some trial data to other minor crops. Leafy vegetables used in this study were: lettuce (Lactuca sativar), leaf broccoli (Brassica oleracea.), chicory (Cichorium intybus.), chinese cabbage (Brassica campestris subsp. napus var. pekinensis), radish (Raphanus sativus), rape (Brassica napus), crown daisy (Chrysanthemum coronarium), edible burdock (Arctium lappa), endive (Cichorium endivia) and mustard greens (Brassica jumcea). Based on the result of control efficacy, all crops were classified into the groups. The results showed the probabilities of extrapolating the control value data of minor crop within the same group. It would be possible to use the pesticides which are already been registered for similar crops to those crops have no registered pesticides.

• Journal of the East Asian Society of Dietary Life
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• v.17 no.5
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• pp.671-677
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• 2007

This study examined the relationship between chlorophyll(Chl) and ascorbic acid(ASA) as an index of freshness for vegetables, when the general consumers purchase several kinds of vegetables at the market. The leaves and fruits of several types of vegetables were divided into top, middle, and basal sections. Chl was determined by a spectrophotometer, and ASA was analyzed by high-performance liquid chromatography. For the vegetable sections, we found that Chl and ASA levels were highest in the top sections, followed by the middle, and were lowest in the basal portions. For the relationship between Chl and ASA in vegetables, there were seven classifications including high Chl(above 80mg/100g of fresh weight) and high ASA(above 80 mg/100 g of fresh weight) for mallow, and kail; the second group had high Chl and low ASA(below 80 mg), such as in spinach, burdock, and perilla leaves; the third group contained medium Chl($40{\sim}80mg$) and medium ASA($40{\sim}80mg$) levels, such as for gonddalbee, angelica, pumpkin leaf and coriander; the fourth group had medium Chl and low ASA levels, as in crown daisy and lettuce; the fifth group contained low Chl(below 40 mg) and high ASA, such as in hot peppers and parsley; the sixth group had low Chl and medium ASA levels, and included broccoli, pak choi, and sweet peppers; finally the seventh group contained low Chl and low ASA levels, as in dropwort, young radishes, shallots, cucumbers and swiss chard.