• Title/Summary/Keyword: Leaf N content

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Antioxidant Activity and Functional Component Analysis of Korean Mountain Ginseng's Different Sections (장뇌삼 부위별 기능성 성분분석과 항산화활성)

  • Kim, Jun-Han;Kim, Jong-Kuk
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.35 no.10
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    • pp.1315-1321
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    • 2006
  • This study was conducted to investigate antioxidant activity and functional component analysis in different sections of Korean mountain ginseng (seed, leaf, stem and root). The major free amino acids were arginine, proline, $\gamma-amino-n-butyric$ acid, alanine and aspartic acid, and proline showed the highest content 22.98 mg/g in 80% MeOH extract of seed. Contents of $\gamma-amino-n-butyric$ acid and alanine were the highest in 80% MeOH extract of leaf, with 26.04 mg/g and 13.07 mg/g. Aspartic acid showed the highest content 23.42 mg/g in 80% MeOH extract of leaf. The major fatty acids were identified as palmitic acid, oleic acid and linoleic acid by GC. Content of total phenolic compounds were 737 mg% in 80% EtOH extract of seed, and 560 mg% in 80% MeOH extract of seed. The highest contents of ferulic acid and salicylic acid were $875{\mu}g/g\;and\;78{\mu}g/g$ in 80% MeOH extract of leaf, and $\rho-coumaric$ acid was $181{\mu}g/g$ in 80% MeOH extract of stem, respectively. The highest contents of ginsenoside-Rg1, Re, Rd and Rc were $14.5{\mu}g/g,\;48.78{\mu}g/g,\;27.57{\mu}g/g\;and\;4.87{\mu}g/g$ in 80% MeOH extract of leaf, respectively. Antioxidant activities by DPPH were 83.82% in water extract of leaf, 89.74% in 80% EtOH extract of leaf and 88.37% in 80% MeOH extract of leaf, and 92.81% in BHA (200 ppm). These results suggest that Korean mountain ginseng is very important as functional food material.

Comparison of Biological Activities of Extracts from Different Parts and Solvent Fractions in Cornus kousa Buerg (산딸나무의 부위별 추출물 및 용매 분획물의 생리활성 비교)

  • Kim, Young-Jung;Jeong, Jin-A;Kwon, Su-Hyun;Lee, Cheol-Hee
    • Korean Journal of Plant Resources
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    • v.21 no.1
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    • pp.28-35
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    • 2008
  • Several extracts of different parts and solvent fractions of Cornus kousa were obtained and their functional material contents, antioxidant activities and tyrosinase inhibition effects were determined. Content of total polyphenols and flavonoids contents in flower were 169.638 $mg{\cdot}g^{-1}$ and 25.418 $mg{\cdot}g^{-1}$, respectively, which were much higher than those of other parts. Also, flower extracts showed the strongest effects on DPPH and ABTS radicals scavenging and ferrous ion chelating. In flower, leaf, and stem extracts, inhibition effects on peroxidation of linoleic acid determined by ferric thiocyanate(FTC) method were higher than a synthetic antioxidant, BHT. Tyrosinase inhibition activities were shown only in flower extract. Flower and leaf extracts, showing high biological activities in various system, were successively reextracted with n-hexane, chloroform, ethylacetate and n-butanol. Total polyphenol contents of water fractions were higher than any other solvent fractions in both flower and leaf, 67.006 $mg{\cdot}g^{-1}$ and 67.739 $mg{\cdot}g^{-1}$, respectively. But total flavonoid contents were higher in ethyl acetate fraction for flower extract and butanol fraction for leaf extract. Among the solvent fractions, the highest efficiency of free radical scavenging activities was obtained in ethyl acetate fraction for flower extract and n-butanol fraction for leaf extract. Tyrosinase inhibition activities were higher in water fraction for both flower and leaf extracts, 49.24% and 31.8%, respectively.

Effect on Nitrous Oxide Emission in Applying Livestock Manure Compost for Strawberry (Fragaria × ananassa Duch.) Cultivation in Plastic Film House (딸기(Fragaria × ananassa Duch.) 시설재배에서 가축분 퇴비 시용이 아산화질소 배출에 미치는 영향)

  • Lee, Chang-Kyu;Moon, Hyung-Cheol;Song, Eun-Ju;Choi, Seon-U;Ko, Do-Young;Chon, Hyong-Gwon;Yun, Seok-In
    • Korean Journal of Organic Agriculture
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    • v.29 no.1
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    • pp.111-123
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    • 2021
  • This study was conducted to investigate the growth characteristics of strawberries and N2O emission by treating the compost for each type of livestock manure, which was an organic farming material, as a basal fertilization in plastic film house. Livestock manure compost, which made from cattle manure, swine manure, and poultry manure as raw materials, were applied to this experiment, treated by mixing or single on the basis of nitrogen content with the standard amount of fertilizer for strawberries. Total emission of N2O were 10.7% higher than those in poultry manure compost treatment compared to the inorganic fertilizer treatment, but 16.5~41.9% lower than those in other livestock manure compost treatment. The period of N2O emission mainly was up to the 17th day after fertilizer application, accounting for 70~87% of the total amount of discharge, and 13~30% of the total amount was emitted for 158 days later. N2O emission was decreased significantly NH4+-N content in the soil, and increased NO3--N. As compared with control, the number of leaves, leaf width and crown diameter of livestock manure compost treatments were not significantly different, leaf length of cattle+poultry, cattle+ swine, swine+poultry treatment higher, and SPAD (soil plant analysis development) values of cattle+poultry treatment highest. There was no significant difference in weight and sugar content of strawberry fruits among treatments.

Effects of pruning intensity and diameter of bearing mother branches on the growth and berry quality in 'Kyoho' grapevines ('거봉' 포도의 전정강도와 결과모지 굵기가 생육 및 과실품질에 미치는 영향)

  • Song, Gi-Cheol
    • Journal of Agricultural Extension & Community Development
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    • v.7 no.1
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    • pp.167-173
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    • 2000
  • This study was carried out to investigate suitable pruning methods to avoid poor berry setting of 'Kyoho' grapevine in Suwon and Anseong areas. Light pruning increased percentage of bud bursts and number of shoots per bearing mother branches(BMB), cluster weight and percentage of berry sating but it decreased shoot length, number of leaves and, leaf area and leaf weight. There was little difference in soluble solids, titratable acidity, and anthocyanin contents of berries by the treatment of either light or heavy pruning. Shoot length became longer as diameter of BMB thickened, leaf area and chlorophyll content also became larger and diameter of BMB. Total carbohydrate and N, P, K, Ca, and Mg contents showed significantly little difference, but total carbohydrate and Ca contents showed a tendency of continuous increase as diameter of BMB thinned. Number of seeded berries per cluster became fewer as BMB thinned but percentage of berry setting showed an increasing tendency. Cluster weight, berry weight, berry number per cluster, soluble solids, anthocyanin content were higher in case of around 1cm of diameters of BMB.

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Estimation of Nutritive Value of Whole Crop Rice Silage and Its Effect on Milk Production Performance by Dairy Cows

  • Islam, M.R.;Ishida, M.;Ando, S.;Nishida, T.;Yoshida, N.
    • Asian-Australasian Journal of Animal Sciences
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    • v.17 no.10
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    • pp.1383-1389
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    • 2004
  • The nutritive value and utilization of whole crop rice silage (WCRS), Hamasari, at yellow mature stage was determined by three studies. In first study, chemical composition, in vivo digestibility and metabolizable energy (ME) content of WCRS was determined by Holstein steers. WCRS contains 6.23% CP, its digestibility is 48.4% and estimated TDN is 56.4%. Its ME content was 1.91 Mcal/kg DM. Gross energy (GE) retention (% of GE intake) in steers is only 22.7% most of which was lost through feces (44.7% of GE intake). It takes 81 minutes to chew a kg of WCRS by steers. In another study, the effect of Hamasari at yellow mature stage at three stages of lactation (early, mid and late lactation) and two levels of concentrate (40 or 60%) on voluntary intake, ME content and ME intake, milk yield and composition using lactating Holstein dairy cows were investigated. Total intake increased with the concentrate level in early and mid lactation, but was similar irrespective of concentrate level in late lactation. WCRS intake was higher with 40% concentrate level than with 60% concentrate. ME intake by cows increased with the concentrate level and WCRS in early lactating cows with 40% concentrate can support only 90% of the ME requirement. Milk production in accordance with ME intake increased with the increase in concentrate level in early and mid lactating cows but was similar in late lactating cows irrespective of concentrate level. Fat and protein percent of milk in mid and late lactating cows were higher with for 60% concentrate than 40%, but reverse was in early lactating cows. Solids-not-fat was higher with for 60% concentrate than 40% concentrate. Finally in situ degradability of botanical fractions such as leaf, stem, head and whole WCRS, Hamasari at yellow mature stage was incubated from 0 to 96 h in Holstein steers to determine DM and N degradability characteristics of botanical fractions and whole WCRS. Both DM and N solubility, rate of degradation and effective degradability of leaf of silage was lower, but slowly degradable fraction was higher compared to stem and head. Solubility of DM and N of stem was higher than other fractions. The 48 h degradability, effective degradability and rate of degradation of leaf were always lower than stem or head. In conclusion, voluntary intake of silage ranged from 5 to 12 kg/d and was higher with low levels of concentrate, but milk yield was higher with high levels of concentrate. Fat corrected milk yield ranged from 19 to 37 kg per day. For consistency of milk, early lactating cows should not be allowed more than 40% whole crop rice silage in the diet, but late lactating cows may be allowed 60% whole crop rice silage.

Effect of Nitrogen Fertilizer Level on the Yield and Quality of Watermelon (Citrullus vulgaris S.) (질소시비수준이 소과종 수박의 수량 및 품질에 미치는 영향)

  • 이상규;김광용;정주호;이용범;배종향
    • Journal of Bio-Environment Control
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    • v.6 no.2
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    • pp.97-102
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    • 1997
  • To investigate the effect of nitrogen level on the yield and quality of watermelon(Citrullus vulgaris S. cv. Bocksubak), N levels of 250, 200, 140, and 0kg/ha with the conventional amount of K and P supply non-fertilization treatments were compared one anther. Plant height, leaf area, fresh weight and dry weight were better in nitrogen application treatments than no nitrogen and non- fertilization treatments. But there was no significant difference between nitrogen levels. Yield and fruit setting ratio were the highest in N level of 140kg/ha. Fruit weight was increased by N application, and soluble solids content was the highest as 12.5 $^{\circ}$Bx in N level of 140kg/ha. Nitrogen content of leaves was increased with the applied nitrogen amount and highest at the middle stage of growth. P content was no significant difference between treatments. Ca content was increased with the applied nitrogen amount and highest at the late stage of growth.

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Growth and Mineral Composition of Young Radish in Soils Amended with Sewage Sludge and Lime (하수오니 및 석회시용이 열무의 생육과 화학성분에 미치는 영향)

  • Jung, Goo-Bok;Kim, Won-Il;Lee, Jong-Sik;Kim, Jin-Ho;Yun, Sun-Gang
    • Korean Journal of Environmental Agriculture
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    • v.22 no.2
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    • pp.87-93
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    • 2003
  • Growth and mineral composition of young radish in soil amended with sewage sludge (ISS) with or without liming were investigated. Levels of EC, T-N, and micronutrients were increased in the ISS treatment (50 Mg/ha) compared to the NPK treatment but those of available P and exchangeable cations were decreased. Chlorosis was appear on the young radish leaf at the ISS treatment, but this phytotoxic symptom was corrected with lime amendment (3 Mg/ha). Yields of young radish leaf and root in the combined treatment of ISS and lime were increased about 29 and 48%, respectively, compared with those in the ISS treatment. Contents of P, K, Ca, and Mg in young radish were higher in the combined treatment to ISS and lime, whereas those contents in the ISS treatment were lower than those in the NPK treatment. Contents of Cu, Zn, and Ni in soil and young radish leaf were positively correlated with total N content in young radish leaf, while contents of P, K, Ca, and Mg were negatively correlated. Contents of Cu, Zn, and Ni in leaf were negatively correlated with yield of young radish. Contents of Cu and Zn in leaf corresponding with a 5% yield reduction of young radish were 22.4 and 349 mg/kg, respectively.

Anti-melanogenesis Effects of Schizophragma hydrangeoides Leaf Ethanol Extracts via Downregulation of Tyrosinase Activity

  • Hyun, Ho Bong;Hyeon, Hye Jin;Kim, Sung Chun;Go, Boram;Yoon, Seon-A;Jung, Yong-Hwan;Ham, Young-Min
    • Korean Journal of Plant Resources
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    • v.34 no.6
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    • pp.510-516
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    • 2021
  • Whitening agents derived from natural sources which do not have side effects are sought after. Schizophragma hydrangeoides is an edible plant that grows wild on Jeju Island. We aimed to determine whether S. hydrangeoides extracts show anti-melanogenic activity. Here, we found that 70% ethanol extracts of S. hydrangeoides leaf suppressed α-melanocyte-stimulating hormone-induced melanogenesis in B16F10 mouse melanoma cells. This activity of anti-melanogenesis in B16F10 cells were investigated by determining melanin content and tyrosinase activity, and by performing western blotting. The 70% ethanol extract downregulated tyrosinase and tyrosinase-related protein 1. In addition, the n-hexane fraction of S. hydrangeoides leaf (HFSH) exhibited significant anti-melanogenic activity among the various solvent fractions tested without reducing the viability of B16F10 cells. Taken together, these results indicate that extracts from S. hydrangeoides leaf can influence cellular processes via modulation of tyrosinase activity. Hence, S. hydrangeoides can be used as a whitening agent in the cosmetic industry and as a therapeutic agent for treating hyperpigmentation disorders in the clinic.

Correlation Between Relative Light Intensity and Physiological Characteristics of Forsythia saxatilis in Bukhansan Natural Habitats (북한산 산개나리의 자생지 내 상대 광량과 생리적 특성간 상관 관계)

  • Han, Sim-Hee;Kim, Gil Nam;Kim, Du-Hyun;Kim, Kyung-Hee
    • Journal of Korean Society of Forest Science
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    • v.101 no.2
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    • pp.236-243
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    • 2012
  • The leaf growth and physiological characteristics of Forsythia saxatilis were investigated at six natural habitats under different light intensities in Bukhansan in order to figure out an appropriate growth environment for conservation and restoration of F. saxatilis that is Korean endemic plant designated as rare and endangered species. Relative light intensities (RLI) at six habitats showed from 10% to 78% of the full sun. Leaf length, leaf width, leaf area and dry leaf weight of population under highest relative light intensity (78%) were the highest. The ratio of dry leaf weight to leaf area increased with the increase of RLI. The content of photosynthetic pigments such as chlorophyll a, b and carotenoid were the highest at 78% of RLI, whereas the ratio of total chlorophyll to carotenoid content reduced according to the increase of RLI. Photosynthetic parameters, such as photosynthetic rate, also increased with the increase of RLI. The total nonstructural carbohydrate (TNC) was 1.5 times higher at 78% of RLI than that at 10% of RLI and the total soluble sugar (TSS) was the highest at 78% of RLI. In conclusion, leaf characteristics and physiological characteristics have high positive correlation with light intensity. Therefore, light condition should be primarily considered to improve growth and physiology characteristics of F. saxatilis under low light intensity.

Effects of Pumpkin (Cucurbita moschata Duch.) Leaf Powder on Quality Characteristics, Antioxidant Activities, and Retarding Retrogradation by Shelf-life of Sponge Cake (호박잎 분말을 첨가한 스폰지 케이크의 품질 특성, 항산화 활성 및 저장 기간에 따른 노화 억제 효과)

  • Song, Ka-Young;O, Hyeonbin;Zhang, Yangyang;Joung, Ki Youeng;Kim, Young-Soon
    • The Korean Journal of Food And Nutrition
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    • v.29 no.6
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    • pp.930-937
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    • 2016
  • This study investigated the quality characteristics, antioxidant activities, and retarding retrogradation of sponge cakes made with 0%, 1%, 3%, 5% and 7% pumpkin (Cucurbita moschata Duch.) leaf powder. Specific capacity significantly increased with the addition (0.33~0.38), but baking loss and dough yield were not significantly different (p<0.05). In color, there was a decrease in the L-value, a-value and b-value, but ${\Delta}E$ increased in proportion to the amount of pumpkin leaf powder. Moisture content increased in the 0~3% additions, from 27.90~31.68%, but decreased in 5% and 7% (22.37% and 28.15%, respectively). pH tended to decrease significantly according to the amount of pumpkin leaf powder (p<0.05). Hardness increased with the addition of pumpkin leaf powder, and pumpkin leaf groups presented higher springiness and cohesiveness than the control. Chewiness was not significantly differ (p<0.05). In retarding retrogradation, Avrami exponent (n) showed that addition of 5% (0.1329) had more retarding retrogradation effect than the control (0.4319), whereas time constant (1/k) showed both 3% (100.00) and 5% (70.42) addition had more effect than control (18.45). Total phenols and flavonoids content increased proportionate to the addition levels. ABTS radical scavenging activity tended to increase according to the level of pumpkin leaf powder. In sensory properties, 5% addition showed maximum color, and 3% addition had the highest scores in flavor, moistness, sweetness, chewiness and overall acceptability. These results suggested that pumpkin leaf was a good addition to improve the quality characteristics, health and sensory preferences. The most appropriate proportion to have the effect of retarding retrogradation in sponge cake is 3%.