• Journal of Microbiology and Biotechnology
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• v.1 no.1
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• pp.31-36
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• 1991

Yeast expression plasmids containing an appropriate leader sequence and bovine $\beta$-casein cDNA were constructed to produce $\beta$-casein for the study of its functional characteristics. Two kinds of expression systems for $\beta$-casein were constructed using pCGYl444 as a precursor plasmid. This plasmid is a yeast-E. coli shuttle vector which contains the chelatin promoter. The plasmid pISB202 contains the invertase leader sequence and $\beta$-casein gene. The plasmid pDEB303 contains the original bovine $\beta$-casein leader sequence gene. These two plasmids were introduced into S. cerevisiae AB116 which is a strain deficient in the major yeast proteinases. Each clone was grown in minimal media for 24 h before induction by $CuSO_4$. The cells were thus grown under expression conditions. Both strains harbouring pISB202 and pDEB303 expressed bovine $\beta$-casein. The $\beta$-casein was detected using immunochemical staining after western blot. Secretion of $\beta$-casein was detected in the culture broth. The estimated amount of secreted $\beta$-casein was approximately 50 ${\MU}g$/l.

• Proceedings of the KIEE Conference
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• 2004.07c
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• pp.1882-1884
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• 2004

In this paper, the waveform parameters of electromagnetic pulses radiated from intracloud discharges were presented. The zero-crossing times and the full pulse widths of the large bipolar electromagnetic pulses due to intracloud discharges are gradually decreased as the stroke proceeds. Also, the electromagnetic pulses tend to be bipolar, with narrow and fast leader pulses superimposed on the initial half cycle. The mean numbers of leader pulses superimposed on the initial half cycle were 1.61${\pm}$0.74 and 1.75${\pm}$0.73 for the positive and negative polarities, respectively.

• The Journal of Asian Finance, Economics and Business
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• v.8 no.12
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• pp.399-409
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• 2021

The demographic characteristics of a leader are important for small businesses during the COVID-19 pandemic. Several researchers have proven that demographic characteristics affect business success. Unfortunately, business success has only been analyzed from a financial perspective and has not been compared to the owner's interests perspective. This study examines business success from both perspectives. This study aims to analyze the demographic characteristics of small entrepreneurs and their impact on business success. Demographics consist of age, experience, education, and gender. Meanwhile, business success is seen from two perspectives: financial performance and the owner's interest. The study used an explanatory survey method. The number of samples is 155 entrepreneurs who are also the leaders of their businesses. The research was conducted in Bandung City, Indonesia, in 2021. The data was processed using descriptive statistics and multiple regression. The study results found that age only affects financial performance. Experience is proven to affect financial performance and the interests of the owner. Gender only affects the interests of the owner. This study concludes that in difficult times such as the COVID-19 pandemic, having young leaders who are experienced will make it easier to achieve business success.

• International journal of advanced smart convergence
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• v.9 no.2
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• pp.212-223
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• 2020

We study to confirm the relationship between leadership trust and job satisfaction on the leadership style of managers recognized by ICT organization members. To this end, the research hypothesis established by using SPSS23.0 for the survey results of 230 ICT organization members was analyzed as follows. First, the hypothesis 1 person leadership type (individual consideration: H1-1, intellectual stimulation: H1-2, charisma: H1-3, conditional compensation: H1-4, exceptional reward: H1-5) had a significant effect on leader trust. The results were obtained. Second, the hypothesis 2 manager's leadership type (individual consideration: H21-1, intellectual stimulation: H2-2, charisma: H2-3, conditional compensation: 2-4, expected compensation: H2-5) is based on job satisfaction. As a result of verifying the impact relationship, all showed significant effect on job satisfaction. Third, it acted as a positive factor in the job satisfaction of the leader of the hypothesis 3-member organizational member. It was confirmed that the job satisfaction of organizational members started based on the trust of actual leaders. Fourth, the role of leadership trust was partially confirmed between leadership type and job satisfaction. Through this study, in order to improve job satisfaction of ICT organizational members, the importance of exerting competency on the leadership type of managers and trusting the leaders on the characteristics consistent with ICT organizational characteristics was emphasized.

• Journal of the Korean Society of Clothing and Textiles
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• v.40 no.4
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• pp.650-659
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• 2016

This study is to comprehend indie fashion which pursues the realization of self-identity, values, freedom and a creation that deviates from conventional cultural flow. This study also aims to understand the definition and characteristics of indie fashion in Korean culture. A rigorous study on indie fashion has not yet been done; therefore, this study was conducted through both a literature study and empirical study. The empirical study asked individuals who engage in indie fashion to provide opinions on the definition and characteristics of indie fashion. The results are as follow. First, indie fashion does not impose conformity upon an individual nor does it pursue differentiation. Second, indie fashion based on self-identity is characterized by an individual's consistent and unchanging style. Third, images (or styles of indie fashion) cannot be uniformly defined as styles different from one individual to another. Fourth, indie fashion can be defined as when an individual perceives themselves as pursuing indie spirit and its attitude. Next was an empirical study that showed in what position indie fashion is and who the fashion leader is in indie fashion. People who enjoy indie fashion said that (regarding indie fashion) it exists independent from the flow of trends as it regards personal values and identity as important.

• Proceedings of the KIEE Conference
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• 2005.07c
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• pp.2140-2142
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• 2005

This paper presents the experimental results on the preliminary breakdown characteristics under a highly non-uniform electric field in $SF_6/CO_2$ gas mixtures. The impulse preliminary breakdown developments are investigated by the measurements of corona current and light emission images. As a result, the preliminary breakdown development mechanisms for both positive and negative polarities are same. The first streamer corona is initiated at the tip of needle electrode, and the leaders develop with a stepwise propagation and bridge the test gap. The pause time of leader pulses in the positive polarity is significantly shorter than that in the negative polarity. Also, the time interval between the first streamer corona onset and breakdown in the negative polarity was much longer than that in the positive polarity The discharge channel paths in the positive polarity were zigzag. On the other hands, the leader channel in the negative polarity was thicker than that in the positive polarity.

• Journal of Korean Society of Forest Science
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• v.88 no.2
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• pp.195-204
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• 1999

To improve nut production and timber quality in a plantation, crown shape controls were suggested for Korean white pines (Pinus koraiensis) under age class III through the analyses of the crown shape controls for seed orchard trees in several species, of crown status of controlled and uncontrolled trees in a Korean white pine seed orchard, and of growth characteristics of plantation trees under age class III. For nut production from the small trees less than 8 m, modified leader type can be applied by cutting the central leader and all branches below 1 m and keeping 4~5 nodes with 3~4 whorled branches a node locating 1 m apart. Trees with a 4~8 m long trunk below the first living branch are shaped into trees for nut and timber production, which have a 4~8 m long knotless stem and a modified leader type crown with 4~5 nodes with 3~4 whorled branches a node at a distance of 1 m. Trees, showing the first living branch at or above 9 m from the surface, are pruned severely for timber production and result in a central leader type containing two fifths of the initial crown. It is advisable to remove the leader and the branches when collecting cones or after/during October to take advantage of easy labor and little resin while cutting. After stem cut, branches showing apical dominance are to be cut to control height when necessary. The removal of the uppermost node may be needed to control the crown shape several years after the initial stem-cut.

• The Journal of Society for e-Business Studies
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• v.21 no.2
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• pp.23-46
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• 2016

With the increasing socio-economic value of an open collaboration over the Internet, it has become significantly important to successfully manage open source software development program. Most of the previous research have focused on various factors that influence the performance of the project, but studies on how the project owners recognized as "leader" affect the outcome of the project are very limited. This research investigates how individual and governance characteristics of an owner influences the performance of project based on impression formation and social capital theory. For a data set, we collect 611 Repositories and the owner's data from the open source development platform Github, and we form knowledge sharing network of an each repository by using social network analysis. We use hierarchical regression analysis, and our results show that a leader, who exposes a lot of one's personal information or who has actively followed and showed interests to communicate with other developers, affects positive impacts on project performance. A leader who has a high centrality in knowledge sharing network also positively affects on project performance. On the other hand, if a leader was highly willing to accept external knowledge or is recognized as an expert in the community with large numbers of followers, the result show negative impacts on project performance. The research may serve as a useful guideline not only for the future open source software projects but also for the effective management of different types of open collaboration.

• Journal of Fashion Business
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• v.17 no.5
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• pp.1-13
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• 2013

The purpose of this study is to examine the power images in the style of a leader: through the focus on the portrait of Henry VIII. Study methods on the literature concerning viewpoints of power and image in the early modern age and the subjects of power are being used for theoretical backgrounds; and the visual data from National Portrait Gallery, British Library and the internet are used for exploratory studies. The results of this study are as follows. First, from the style of Henry VIII who represented the image of political power, the modern political phenomenon can be defined. Such phenomenon which displays images of political leaders from the study indicate an effective strategy. Second, the religious, a diplomatic representation for the style of Henry VIII powers, such as costume style and somatic visual images, represents the dignity and power by delivering visible internal attributes. Thus, the role model recognized by the society and the public's perception of leadership style affects the acknowledgement and belief by the people.

• Journal of Information Technology Applications and Management
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• v.26 no.3
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• pp.43-59
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• 2019

The goal of this paper is to investigate changes in North Korea's domestic and foreign policies through automated text analysis over North Korean new year addresses, one of most important and authoritative document publicly announced by North Korean government. Based on that data, we then analyze the status of text mining research, using a text mining technique to find the topics, methods, and trends of text mining research. We also investigate the characteristics and method of analysis of the text mining techniques, confirmed by analysis of the data. We propose a procedure to find meaningful tendencies based on a combination of text mining, cluster analysis, and co-occurrence networks. To demonstrate applicability and effectiveness of the proposed procedure, we analyzed the inaugural addresses of Kim Jung Un of the North Korea from 2017 to 2019. The main results of this study show that trends in the North Korean national policy agenda can be discovered based on clustering and visualization algorithms. We found that uncovered semantic structures of North Korean new year addresses closely follow major changes in North Korean government's positions toward their own people as well as outside audience such as USA and South Korea.