• Asian-Australasian Journal of Animal Sciences
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• 제27권8호
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• pp.1075-1081
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• 2014

CRBP1 (cellular retinol binding protein 1) and CRBP3 (cellular retinol binding protein 3), are important components of the retinoid signaling pathway and take part in vitamin A absorption, transport and metabolism. Based on the role of vitamin A in chicken laying performance, we investigated the polymorphism of CRBP1 and CRBP3 genes in 349 chickens using single strand conformation polymorphism and DNA sequencing methods. Only one polymorphism was identified in the third intron of CRBP1, two polymorphisms were detected in CRBP3; they were located in the second intron and the third intron respectively. The association studies between these three SNPs and laying performance traits were performed in Erlang mountainous chicken. Notably, the SNP g.14604G>T of CRBP1 was shown to be significantly associated with body weight at first egg (BWFE), age at first egg (AFE), weight at first egg (WFE) and total number of eggs with 300 age (EN). The CRBP3 polymorphism g.934C>G was associated with AFE, and the g.1324A>G was associated with AFE and BWFE, but none of these polymorphisms were associated with egg quality traits. Haplotype combinations constructed on these two SNPs of CRBP3 gene were associated with BWFE and AFE. In particular, diplotype H2H2 had positive effect on AFE, BWFE, EN, and average egg-laying interval. We herein describe for the first time basic research on the polymorphism of chicken CRBP1 and CRBP3 genes that is predictive of genetic potential for laying performance in chicken.

• 한국환경생태학회지
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• 제24권4호
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• pp.345-350
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• 2010

1988년부터 2007년 사이에 수행된 붉은머리오목눈이(Paradoxornis webbianus)의 생태연구 과정에서 종내탁란으로 추정되는 현상은 4개 둥지(0.38%, n=1,062)에서 관찰되었다. 첫 번째는 1995년 경기도 양평군 부용리에서 붉은머리오목눈이의 한배산란수의 범위를 초과하는 9개의 알이 있는 둥지가 관찰되었다. 두 번째와 세 번째는 2001년과 2005년, 경기도 안산시 사동에서 하루 사이에 2개의 알이 산란된 둥지가 각각 관찰되었다. 마지막은 2007년 부용리에서 어미새의 산란 전에 어미새의 알과는 색이 다른 알이 하나 산란된 둥지가 발견되었다. 이러한 결과는 비록 낮은 빈도이지만 붉은머리오목눈이에서 종내탁란 현상이 나타나고 있음을 보여준다.

• 한국가금학회지
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• 제31권1호
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• pp.37-44
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• 2004

It has been recognized that the hen, like its mammalian counterparts, provides young chicks with antibodies as protection against hostile invaders. This system facilitates the transfer of specific antibodies from serum to egg yolk, and provides a supply of antibodies called immunoglobulin Y(IgY) to the developing embryo and the hatched chick. The protection against pathogens that the relatively immune-incompetent newly hatched chick has, is through transmission of antibodies from the mother via the egg. Egg yolk, therefore, can be loaded with a large amount of IgY against pathogens which can immobilize the existing or invading pathogens during the embryo development or in day-old chicks. Thus, the immunization of laying hens to various pathogens results in production of different antigen-specific IgY in eggs. Egg yolk contains 8∼20 mg of jmmunoglobulins (IgY) per ml or 136∼340 mg per yolk suggesting that more than 30 g of IgY can be obtained from one immunized hen in a year. By immunizing laying hens with antigens and collecting IgY from egg yolk, low cost antibodies at less than $10 per g compared to more than $20,000 per g of mammalian IgG can be obtained. This IgY technology opens new potential market applications in medicine, public health, veterinary medicine and food safety. A broader use of IgY technology could be applied as biological or diagnostic tool, nutraceutical or functional food development, oral-supplementation for prophylaxis, and as pathogen-specific antimicrobial agents for infectious disease control. This paper has emphasized that when IgY-loaded chicken eggs are produced and consumed, the specific antibody binds, immobilizes and consequently reduces or inhibits the growth or colony forming abilities of microbial pathogens. This concept could serve as an alternative agent to replace the use of antibiotics, since today, more and more antibiotics are less effective in the treatment of infections, due to the emergence of drug-resistant bacteria.

• 한국가금학회지
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• 제32권3호
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• pp.219-224
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• 2005

본 연구는 산란계에서 장석의 첨가가 난 생산과 난 성분에 미치는 영향을 구명하기 위하여 실시하였다. 공시동물은 갈색테트라 산란계 96수를 4처리 4반복으로 반복당 6수씩 3단 철제 cage에서 사양시험을 실시하였다. 시험사료는 대조구를 기초사료로 이용하였으며, 장석을 수준별로 $0.5\%,\;1.0\%$ 및 $1.5\%$로 첨가하여 배합하였다. 산란율은 대조구와 비교하여 장석 $0.5\%$와 $1.0\%$ 첨가구에서 증가하였으며, 통계적인 유의차를 보였다(P<0.05). 장석 0.5, 1.0및 $1.5\%$첨가구에서 산란계의 난중, 산란량, 사료섭취량, 사료요구율, 난각강도 및 난황색은 대조구에 비해 유의차가 없었다(P>0.05). 계란의 하우 유니트와 혈반은 장석 $1.5\%$ 첨가구에서 유의적으로 증가하였으며(P<0.05), 난백 높이와 잔반은 유의차를 보이지 않았다(P>0.05). 관능검사 결과 다즙성은 대조구가 2.78로 가장 낮았고, 장석 $1.0\%$ 첨가구가 3.57로 가장 높아 통계적 유의차를 보였다(P<0.05).종합적인 기호도에서 장석 $1.0\%$ 첨가구가 3.70으로 가장 높은 기호도를 보였다.

• 한국환경과학회지
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• 제29권7호
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• pp.785-788
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• 2020

We investigated the oviposition characteristics of Protaetia brevitarsis seulensis, focusing on zone breeding throughout the year in terms of the development and utilization of insect resources. To select Protaetia brevitarsis seulensis individuals laying a large number of eggs, the number of eggs laid for each individual was measured for each individual after emergence as adults from larvae grown at room temperature in five farmhouses. The five study zone included Gongju (Tancheon), Gyeonggi (Himsen), Chungnam (Gyeryong), Taean (Wonbuk), and Siheung (Baekse). The average oviposition tendency during a 12-week period obtained from the five regions was somewhat different; however, there was no remarkable difference in overall tendency. The maximum oviposition in Protaetia brevitarsisseulensis in the five regions occurred between 6 and 7 weeks. Compared toother regions, the average cumulative number of eggs laid during the 12-week period was higher (40%-60%) in Gongju (Tancheon) and Gyeonggi (Himsen). These results indicate that in two regions, zone breeding is selected as a system for increasing the demand for insects.

• 한국식품영양과학회지
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• 제15권3호
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• pp.306-312
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• 1986

Demonstration of the highly positive correlation between blood cholestrol levels and heart disease has made consumers wary of the fats in meat, milk and eggs. The egg, as perhaps the single largest common source of cholesterol, has been cited by many members of the medical and scientific world as a food contributing to heart disease. In light of decreasing per capita egg consumption and continuing dietary egg cholesterol controversy, many researchers have focused their efforts on egg nutrition. The results reported, however, are often contradictory. In spite of the disputable scientific evidence, the egg has been labelled (erroneously) as a highly cholesterogenic food. The objective of this presentation is to present a general picture of the problem and discuss our laboratory findings relevant to the problem. An isotope technique was utilized to incorporate $^{14}C$-cholesterol into egg yolk lipoproteins and study the metabolic fate of dietary ovo-cholesterol in rats. Two hundred and fifty micro-curies of 4-$^{14}C$-cholesterol, emulsified in corn oil, were orally administered to five Single Comb White Leghorn laying hens. Eggs were collected, hard-boiled, and the hot dried egg yolk powder (HEY) was prepared. Total radioactivity excreted via feces was determined. The rat groups fed egg yolk powder excreted more than 95% of the ingested ovo-cholesterol, whereas the rat chow group excreted only 47%. No difference was observed between HEY and CEY treatments. Therefore, an unknown lipid factor present in egg folk accelerates cholesterol turnover rate and excretion via feces.

• Asian-Australasian Journal of Animal Sciences
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• 제31권5호
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• pp.705-711
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• 2018

Objective: This experiment was conducted to evaluate the effects of golden needle mushroom (Flammulina velutipes) stem waste (FVW), on organic eggs production, calcium utilization, antibody response, serum immunoglobulin, and serum cytokine concentration at early phase of production in laying hens. Methods: A total 210, 19 weeks old aged ISA Brown layers were randomly assigned into 5 equal treatment groups, with 7 replications of 6 hens each. Dietary treatment included a standard basal diet as control; antibiotic (0.05% flavomycin); 2% FVW; 4% FVW; and 6% FVW. The experimental duration was 10 weeks. Results: There was no significant differences (p>0.05) on hen day egg production, egg weight, egg mass, feed intake, and feed conversion ratio (FCR) among experimental groups. Unmarketable eggs were significantly lower (p<0.05) both in 4% FVW and 6% FVW fed groups than control group. The calcium retention and calcium in egg shell deposition were significantly higher (p<0.05) in FVW inclusion groups than control and antibiotic groups. Antibody titers against Newcastle diseases were significantly higher (p<0.05) in 6% FVW fed group (except combined with 4% FVW at day 147) and infectious bronchitis were significantly higher (p<0.05) in FVW fed groups (except 2% FVW and 4% FVW at day 161) than control and antibiotic groups. Serum immunoglobulin sIgA was significantly higher (p<0.05) in all levels of FVW and IgG was significantly higher (p<0.05) in 4% FVW than control and antibiotic groups. Serum cytokine concentration interleukin-2 (IL-2) was significantly higher (p<0.05) in 6% FVW; IL-6 and tumor necrotic $factor-{\alpha}$ were significantly higher (p<0.05) both in 4% FVW and 6% FVW than control and antibiotic groups; IL-4 was significantly higher (p<0.05) in antibiotic, 2% FVW and 4% FVW fed groups than control. Conclusion: F. velutipes mushroom waste can be used as a novel substitute for antibiotic for organic egg production and sound health status in laying hens.

• 한국가금학회지
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• 제46권2호
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• pp.87-94
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• 2019

본 연구를 통해 감껍질의 산란계 사료 자원으로써의 가치를 평가하였다. 감껍질은 기본 사료에 비해 조단백질과 조지방의 비율은 적지만 조섬유의 비율은 높은 것으로 나타났다. 사료에 감껍질을 2%와 5% 첨가하여 급이한 결과, 모두 산란율에 아무런 영향을 주지 않았다. 또한 감껍질 급이가 계란의 pH, Haugh unit, 난황색 및 난각두께에도 대체로 아무런 영향을 주지 않아, 계란 품질 변화도 유발하지 않았다. 계란내 영양 성분을 분석한 결과에서는 감껍질의 급이가 계란 내 아미노산 성분의 변화에 아무런 영향을 주지 않았으며, 지방산의 경우 감껍질에 많은 linolenic acid가 난황에 전이되어 증가하는 결과를 나타냈다. 이상에 감껍질을 최대 5%까지 산란계 사료에 이용 가능할 것으로 사료되며, 이는 농가 소득 증대에도 도움이 될 것으로 사료된다.

• Animal Bioscience
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• 제36권3호
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• pp.484-491
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• 2023

Objective: The aim of this trial was to investigate the effect of supplementation and withdrawal of selenium-enriched kale sprouts (SeKS) on productivity and egg Se concentration of laying hens. Selenium from commercial Se-enriched yeast (SeY) was used as a comparative Se source. Methods: One-hundred and eighty 61-week-old laying hens were randomly divided into 5 treatment groups with 4 replicates (9 hens each) in a 2×2+1 Augmented Factorial Experiment in a completely randomized design. The experimental diets were basal diet, basal diet supplemented with 0.2 and 0.4 mg Se/kg from SeKS and SeY, respectively. The 8-week feeding trial was divided into 2 periods, namely the Se supplemental period (week 1 to 4) and the Se withdrawal period (week 5 to 8). Results: Productive performance, egg quality and egg Se concentration of laying hens were not affected by sources of Se (SeKS and SeY) during both, the Se supplemental and withdrawal periods. Egg production and egg Se concentration increased (p<0.05) with increasing levels of Se supplementation. The egg Se concentration increased and reached a peak 1 week after Se supplementation. However, concentration of Se in eggs of hens fed Se from both sources decreased rapidly from the second week of the Se withdrawal period to reach the same egg Se concentration of hens fed the basal diet by the fourth week of the Se withdrawal period. Conclusion: The efficacy of Se from SeKS on productivity and egg Se concentration in laying hens was comparable to commercial SeY. Thus, SeKS can provide an alternate organic Se source for production of Se-enriched eggs.

• 한국가금학회지
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• 제30권2호
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• pp.95-99
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• 2003

본 연구는 육용종계 암컷과수컷의 배웅비를 달리할 경우 종란의 수정율과 부화율에 미치는 영향을 구명하고자 실시 하였다. 육용종계 암컷과 수컷 비율이 각각 9:I(암컷:수컷＝11:99), 11:1(9:99), 및 13:1(8:104) 이 되도록 하여 3.2 ${\times}$ 6.4m 크기의 pen에 사육하였다. 각 처리당 3반복으로, 30주령된 Ross 종계 수컷 112수 암컷 1,208수를 시험에 공시하였다. 30주령시 종란의 수정율은 93 ∼ 94% 내외로 처리간 유의차가 발견되지 않았다.