• Title/Summary/Keyword: Layer-by-Layer

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Optimization and Scale-up of Fish Skin Peptide Loaded Liposome Preparation and Its Storage Stability (어피 펩타이드 리포좀 대량생산 최적 조건 및 저장 안정성)

  • Lee, JungGyu;Lee, YunJung;Bai, JingJing;Kim, Soojin;Cho, Youngjae;Choi, Mi-Jung
    • Food Engineering Progress
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    • v.21 no.4
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    • pp.360-366
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    • 2017
  • Fish skin peptide-loaded liposomes were prepared in 100 mL and 1 L solution as lab scales, and 10 L solution as a prototype scale. The particle size and zeta potential were measured to determine the optimal conditions for the production of fish skin peptide-loaded liposome. The liposome was manufactured by the following conditions: (1) primary homogenization at 4,000 rpm, 8,000 rpm, and 12,000 rpm for 3 minutes; (2) secondary homogenization at 40 watt (W), 60 W, and 80 W for 3 minutes. From this experimental design, the optimal conditions of homogenization were selected as 4,000 rpm and 60 W. For the next step, fish peptides were prepared as the concentrations of 3, 6, and 12% at the optimum manufacturing conditions of liposome and stored at $4^{\circ}C$. Particle size, polydispersion index (pdI), and zeta potential of peptide-loaded liposome were measured for its stability. Particle size increased significantly as manufacture scale and peptide concentration increased, and decreased over storage time. The zeta potential results increased as storage time increased at 10 L scale. In addition, 12% peptide showed the formation of a sediment layer after 3 weeks, and 6% peptide was considered to be the most suitable for industrial application.

The Study on the Embedded Active Device for Ka-Band using the Component Embedding Process (부품 내장 공정을 이용한 5G용 내장형 능동소자에 관한 연구)

  • Jung, Jae-Woong;Park, Se-Hoon;Ryu, Jong-In
    • Journal of the Microelectronics and Packaging Society
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    • v.28 no.3
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    • pp.1-7
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    • 2021
  • In this paper, by embedding a bare-die chip-type drive amplifier into the PCB composed of ABF and FR-4, it implements an embedded active device that can be applied in 28 GHz band modules. The ABF has a dielectric constant of 3.2 and a dielectric loss of 0.016. The FR-4 where the drive amplifier is embedded has a dielectric constant of 3.5 and a dielectric loss of 0.02. The proposed embedded module is processed into two structures, and S-parameter properties are confirmed with measurements. The two process structures are an embedding structure of face-up and an embedding structure of face-down. The fabricated module is measured on a designed test board using Taconic's TLY-5A(dielectric constant : 2.17, dielectric loss : 0.0002). The PCB which embedded into the face-down expected better gain performance due to shorter interconnection-line from the RF pad of the Bear-die chip to the pattern of formed layer. But it is verified that the ground at the bottom of the bear-die chip is grounded Through via, resulting in an oscillation. On the other hand, the face-up structure has a stable gain characteristic of more than 10 dB from 25 GHz to 30 GHz, with a gain of 12.32 dB at the center frequency of 28 GHz. The output characteristics of module embedded into the face-up structure are measured using signal generator and spectrum analyzer. When the input power (Pin) of the signal generator was applied from -10 dBm to 20 dBm, the gain compression point (P1dB) of the embedded module was 20.38 dB. Ultimately, the bare-die chip used in this paper was verified through measurement that the oscillation is improved according to the grounding methods when embedding in a PCB. Thus, the module embedded into the face-up structure will be able to be properly used for communication modules in millimeter wave bands.

Global Ocean Data Assimilation and Prediction System in KMA: Description and Assessment (기상청 전지구 해양자료동화시스템(GODAPS): 개요 및 검증)

  • Chang, Pil-Hun;Hwang, Seung-On;Choo, Sung-Ho;Lee, Johan;Lee, Sang-Min;Boo, Kyung-On
    • Atmosphere
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    • v.31 no.2
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    • pp.229-240
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    • 2021
  • The Global Ocean Data Assimilation and Prediction System (GODAPS) in operation at the KMA (Korea Meteorological Administration) is introduced. GODAPS consists of ocean model, ice model, and 3-d variational ocean data assimilation system. GODAPS assimilates conventional and satellite observations for sea surface temperature and height, observations of sea-ice concentration, as well as temperature and salinity profiles for the ocean using a 24-hour data assimilation window. It finally produces ocean analysis fields with a resolution of 0.25 ORCA (tripolar) grid and 75-layer in depth. This analysis is used for providing a boundary condition for the atmospheric model of the KMA Global Seasonal Forecasting System version 5 (GloSea5) in addition to monitoring on the global ocean and ice. For the purpose of evaluating the quality of ocean analysis produced by GODAPS, a one-year data assimilation experiment was performed. Assimilation of global observing system in GODAPS results in producing improved analysis and forecast fields with reduced error in terms of RMSE of innovation and analysis increment. In addition, comparison with an unassimilated experiment shows a mostly positive impact, especially over the region with large oceanic variability.

Water quality characteristics and spatial distribution of phytoplankton during dry and rainy seasons in Bunam Lake and Cheonsu Bay, Korea (부남호·천수만의 갈수기와 강우기 수질 오염 특성과 식물플랑크톤의 공간 분포 특성)

  • Lee, Minji;Seo, Jin Young;Baek, Seung Ho
    • Korean Journal of Environmental Biology
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    • v.39 no.2
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    • pp.184-194
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    • 2021
  • Since the construction of a dike in 1983, the water quality in the Bunam Lake has continued to deteriorate due to algal bloom caused by agricultural nutrient loading. Therefore, we evaluated the change in water quality and phytoplankton ecological characteristics in Bunam Lake and Cheonsu Bay, Korea. Water temperature, salinity, dissolved oxygen, chemical oxygen demand (COD), chlorophyll, and phytoplankton community were surveyed in April during the dry season and in July during the rainy reason. As a result, during the dry period, phytoplankton proliferated greatly and stagnated in the Bunam Lake while a very high population of cyanobacteria Oscillatoria spp. (8.61×107 cells L-1) was recorded. Most of the nutrients, except, nitrate and nitrite, were consumed due to the large growth of phytoplankton. However, during the rainy period, concentrations of ammonia, phosphate, silicate, nitrate, and nitrite, were very high towards the upper station due to the inflow of fresh water. Cyanobacteria Oscillatoria and Microcystis spp. were dominant in the Bunam Lake during the rainy period. Even in the Cheonsu Bay, cyanobacteria dominated due to the effect of discharge and diatoms, such as, Chaetoceros spp. and Eucampia zodiacus, which also proliferated significantly due to increased levels of nutrients. Since the eutrophication index was above 1 in Bunam Lake, it was classified as eutrophic water and the Cheonsu Bay was classified as eutrophic water only during the rainy season. In addition, a stagnant seawater-derived hypoxia water mass was observed at a depth of8m in the Bunam Lake adjacent to the tide embankment and the COD concentration reached 206 mg L-1 in the bottom layer at B3. Based on this result, it is considered that the water quality will continue to deteriorate if organic matters settle due to continuous inflow of nutrients and growth of organisms while the bottom water mass is stagnant.

Establishment of A WebGIS-based Information System for Continuous Observation during Ocean Research Vessel Operation (WebGIS 기반 해양 연구선 상시관측 정보 체계 구축)

  • HAN, Hyeon-Gyeong;LEE, Cholyoung;KIM, Tae-Hoon;HAN, Jae-Rim;CHOI, Hyun-Woo
    • Journal of the Korean Association of Geographic Information Studies
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    • v.24 no.1
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    • pp.40-53
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    • 2021
  • Research vessels(R/Vs) used for ocean research move to the planned research area and perform ocean observations suitable for the research purpose. The five research vessels of the Korea Institute of Ocean Science & Technology(KIOST) are equipped with global positioning system(GPS), water depth, weather, sea surface layer temperature and salinity measurement equipment that can be observed at all times during cruise. An information platform is required to systematically manage and utilize the data produced through such continuous observation equipment. Therefore, the data flow was defined through a series of business analysis ranging from the research vessel operation plan to observation during the operation of the research vessel, data collection, data processing, data storage, display and service. After creating a functional design for each stage of the business process, KIOST Underway Meteorological & Oceanographic Information System(KUMOS), a Web-Geographic information system (Web-GIS) based information platform, was built. Since the data produced during the cruise of the R/Vs have characteristics of temporal and spatial variability, a quality management system was developed that considered these variabilities. For the systematic management and service of data, the KUMOS integrated Database(DB) was established, and functions such as R/V tracking, data display, search and provision were implemented. The dataset provided by KUMOS consists of cruise report, raw data, Quality Control(QC) flagged data, filtered data, cruise track line data, and data report for each cruise of the R/V. The business processing procedure and system of KUMOS for each function developed through this study are expected to serve as a benchmark for domestic ocean-related institutions and universities that have research vessels capable of continuous observations during cruise.

Construction Techniques of Earthen Fortifications in the Hanseong Period of Baekje Kingdom (백제 한성기 토성의 축조기술)

  • LEE, Hyeokhee
    • Korean Journal of Heritage: History & Science
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    • v.55 no.2
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    • pp.168-184
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    • 2022
  • This paper examined the construction techniques of the earthen fortifications in the Hanseong Period of Baekje Kingdom, which has been researched most frequently among the Three Kingdoms. The construction processes of the Earthen Fortifications were reviewed and dividing into 'selection of location and construction of the base', 'construction of the wall', and 'finish, extension and repair'. The results show that various techniques were mobilized for building these earthen fortifications. Techniques which were adequate for the topography were utilized for reinforcing the base, and several other techniques were used for constructing the wall. In particular, techniques for wall construction may be clearly divided into those of the fill(盛土) and panchuk(版築) techniques. The fill method has been assumed since the 2000s to have been more efficient than the panchuk technique. This method never uses the structure of the panchuk technique and is characterized by a complex soil layer line, an alternate fill, use of 'earth mound(土堤)'/'clay clod(土塊)', and junctions of oval fill units. The fill method allows us to understand active technological sharing and application among the embankment structures in the period of the Three Kingdoms. The panchuk technique is used to construct a wall using a stamped earthen structure. This technique is divided into types B1 and B2 according to the height, scale, and extension method of the structure. Type B1 precedes B2, which was introduced in the late Hanseong Period. Staring with the Pungnap Earthen Fortification in Seoul, the panchuk technique seems to have spread throughout South Korea. The techniques of the fill and panchuk techniques coexisted at the time when they appeared, but panchuk earthen fortifications gradually dominated. Both techniques have completely different methods for the soil layers, and they have opposite orders of construction. Accordingly, it is assumed that both have different technical systems. The construction techniques of the earthen fortifications began from the Hanseong Period of Baekje Kingdom and were handed down and developed until the Woongjin-Sabi Periods. In the process, it seems that there existed active interactions with other nations. Recently, since studies of the earthen fortifications have been increasing mainly in the southern areas, it is expected that comparative analysis with neighboring countries will be done intensively.

A Study on Evaluating the Possibility of Monitoring Ships of CAS500-1 Images Based on YOLO Algorithm: A Case Study of a Busan New Port and an Oakland Port in California (YOLO 알고리즘 기반 국토위성영상의 선박 모니터링 가능성 평가 연구: 부산 신항과 캘리포니아 오클랜드항을 대상으로)

  • Park, Sangchul;Park, Yeongbin;Jang, Soyeong;Kim, Tae-Ho
    • Korean Journal of Remote Sensing
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    • v.38 no.6_1
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    • pp.1463-1478
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    • 2022
  • Maritime transport accounts for 99.7% of the exports and imports of the Republic of Korea; therefore, developing a vessel monitoring system for efficient operation is of significant interest. Several studies have focused on tracking and monitoring vessel movements based on automatic identification system (AIS) data; however, ships without AIS have limited monitoring and tracking ability. High-resolution optical satellite images can provide the missing layer of information in AIS-based monitoring systems because they can identify non-AIS vessels and small ships over a wide range. Therefore, it is necessary to investigate vessel monitoring and small vessel classification systems using high-resolution optical satellite images. This study examined the possibility of developing ship monitoring systems using Compact Advanced Satellite 500-1 (CAS500-1) satellite images by first training a deep learning model using satellite image data and then performing detection in other images. To determine the effectiveness of the proposed method, the learning data was acquired from ships in the Yellow Sea and its major ports, and the detection model was established using the You Only Look Once (YOLO) algorithm. The ship detection performance was evaluated for a domestic and an international port. The results obtained using the detection model in ships in the anchorage and berth areas were compared with the ship classification information obtained using AIS, and an accuracy of 85.5% and 70% was achieved using domestic and international classification models, respectively. The results indicate that high-resolution satellite images can be used in mooring ships for vessel monitoring. The developed approach can potentially be used in vessel tracking and monitoring systems at major ports around the world if the accuracy of the detection model is improved through continuous learning data construction.

Initial Analysis of the Underground Air Among Jeju Lava Forest(Sumgol) and its Healing Effect on the Human Body (제주 현무암 '숲' 지하 공기(숨골: Sumgol)의 분석과 인체에 미치는 치유 효과)

  • Sin, SBangsik;Kim, Hyek Nyeon;Lee, Deok Hee;Kim, Tae Seung;Kim, Yong Hwan;Kang, Chang Hee;Song, Kyu Jin;Lee, Hyung H.
    • Journal of Naturopathy
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    • v.11 no.1
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    • pp.18-30
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    • 2022
  • Background: It was to develop an air purification system (APS) using an underground air purification layer to verify the effect of basalt forest's underground air (sumgol) on a volcanic Jeju. Finally, it is necessary to analyze these purified air components and their usefulness to the human body in an air experience center. Purpose: It was to collect basalt forest air, analyze its composition, and explore its effect on the human body. Methods: We APS devices installed at four points in the Papaville area of Jeju. The air discharged from the APS was collected and analyzed the recycling components. An installed experience room filled with negative ions is about 5,000 ions/m3. After allowing the participants to stay for 60 to 120 minutes, we investigated the state of blood vessels. Results: In the analysis of the underground air, the O2 concentration was 21.18%, which was higher than the average oxygen concentration of 20.94% in the atmosphere. However, Formaldehyde was not detected, and the CO2 was 419 ppm, which was lower than that of indoor air. The PM2.5 concentration was less than 24 ㎍/m3 and detected anions over 5.000 /m3. The experiencer's vascular states improved, and the increase in pulse rate and stress relief were high. Conclusions: The valuable ingredients identified by analyzing the air were precious for natural healing. The experience results showed that it effectively improved the pulse rate, blood vessels, and stress. These conditions may be highly beneficial as a new area for expanding the basalt lava forest in the Jeju area into the natural healing and wellness industry.

The Types of Warm Temperate Forest and the Degraded Levels in the Island Area of the West and South Coast (서남해안 도서지역의 난온대 식생유형화 및 훼손등급)

  • Park, Seok-Gon;Sung, Chan-Yong;Kang, Hyun-Mi
    • Korean Journal of Environment and Ecology
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    • v.35 no.6
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    • pp.579-593
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    • 2021
  • In order to understand the types of vegetation in warm temperate-climate zones, vegetation was investigated in several island areas in Jeollanam-do (Jindo, Wando, Gangjin, Goheung, and Yeosu). The evaluation standard for degraded level of warm temperate forests were proposed based on the importance percentage (IP) in canopy layer of the evergreen broad-leaf forests and the number of arboreal evergreen broad-leaf species. Through these measurements, the restoration types and techniques for each degraded level were estimated, and it is intended to be used in establishing restoration plans for the southwest coast island area. The vegetation was analyzed using the two-way indicator species analysis (TWINSPAN) method using survey data of 307 plots. As a result, it was divided into 8 communities, and the appearance characteristics of evergreen broad-leaf species were identified in each community. Community I was located on the lower slope at an altitude of 86.6 m, and Neolitsea sericea and Castanopsis sieboldii were dominant. Communities II and III were the vegetation types that appear on the coast below an altitude of 10.5 to 22.5 m, and Machilus thunbergii, Cinnamomum japonicum, N. sericea, and C. sieboldii were dominant. Communities IV and V were vegetation types that appeared in the lower and middle slops between the altitudes of 71.9 to 153.4m, and C. sieboldii was dominant. In community VI, the N. sericea was dominant in the lower and middle slops at an altitude of 166.9 m. The last communities VII and VIII were the vegetation types that appeared on the middle slop at an altitude of 187.8 to 246.2 m. Also, Quercus acuta and Q. salicina were present. In summary, the evergreen broad-leaf forests dominated by M. thunbergii, C. japonicum, and N. sericea appeared mainly in the coastal areas of the lowlands. The community of C. sieboldii was distributed higher inland than this community. The communities that appeared mainly in the inland highlands at levels above these two communities were Q. acuta and Q. salicina. The degraded level was classified as 0 to V, according to the IP of arboreal evergreen broad-leaf species and the number of arboreal evergreen broad-leaf species. According to the degraded level, the restoration types (preservation, induction, improvement, creation) and the restoration techniques were determined.

A Characteristics and Management Plan of Elaeocarpus sylvestris var. ellipticus Population Distributed in Munseom(island), a Natural Monument (천연기념물 문섬의 담팔수 개체군 특성 및 관리방안)

  • Choi, Byoung-Ki;Lee, Ho-Sang;Seo, Yeon-Ok;Choi, Hyung-Soon;Yang, Ju-Eun;Song, Kuk-Man;Song, Gwan-Pil
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.39 no.1
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    • pp.37-42
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    • 2021
  • Munseom (Island) is known as the largest native Elaeocarpus sylvestris var. ellipticus in Korea. Recently, disease damage from the E. sylvestris var. ellipticus community has been reported in Munseom. This study was conducted to understand the damage situation and growth characteristics of the E. sylvestris var. ellipticus population in Munseom. The purpose of this study was to evaluate the spontaneous regeneration potential of the community by grasping the characteristics of the population of surviving E. sylvestris var. ellipticus individuals, and to discuss ways to restore native habitats. A total population survey was conducted to determine the distribution status of E. sylvestris var. ellipticus individuals. The field survey carefully recorded the height of tree, DBH, DRH, and growing status, along with GPS location information of the individual. The growth status of E. sylvestris var. ellipticus individuals distributed in Munseom and the characteristics of tree height, DBH, DRH of each individual were analyzed. The total number of E. sylvestris var. ellipticus populations identified in Munseom was 293. The dense E. sylvestris var. ellipticus community reported in 2005 has been identified as being greatly damaged or damaged. In particular, the damage was more serious in mature trees with a height of 6m or more forming the canopy layer in the forest, and it was confirmed that 80.6% of the mature trees died. In the growth characteristics of the old-growth tree, which is an indicator of the health of the forest based on DRH, 75.4% died or the growth condition was found to be poor. In order to restore the native habitat of E. sylvestris var. ellipticus in Munseom, conservation efforts such as pest control, preservation of mature trees, control of the territory of young trees, and reintroduction after ex-situ transplantation etc. are considered necessary.