• 대한기계학회논문집B
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• 제34권1호
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• pp.67-71
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• 2010

1 GW/$cm^2$ 이상의 고강도의 레이저 빔을 얇은 금속 호일의 한 점에 집중시키면, 레이저 삭마현상에 의해 발생된 충격파가 금속 호일 안으로 전파하게 된다. 이 충격파는 금속 호일의 반대 면에서 팽창파로 반사되고, 그 과정에서 금속 호일에 급격한 변형이 일어난다. 이 때, 금속 호일의 반대 면에 미세한 마이크로 단위 크기의 입자들을 코팅하면, 금속의 순간적인 변형으로 인해 입자들이 큰 운동량을 얻으며 가속되어 빠른 속도로 튕겨져 나가게 되는데 이것이 바이오리스틱 약물 전달의 원리이다. 이번 연구에서는 바이오리스틱 시스템의 제어성, 안정성, 효율을 향상시키고자 컨파인 조건을 변화시키며, 인체의 연한 조직을 모사하는 3% 젤라틴 용액으로의 침투 모습을 파악하였다. 사용한 컨파인 매질은 BK7 유리, 물, 그리고 초음파젤(RHAPAPHRM Co. Ltd)이다. 실험결과, 컨파인 매질과 그 두께를 조절함으로써 마이크로 입자들의 침투 양상을 제어할 수 있음을 확인하였다.

• Molecules and Cells
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• 제28권3호
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• pp.175-181
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• 2009

In hypertriglyceridaemic individuals, atherosclerogenesis is associated with the increased concentrations of very low density lipoprotein (VLDL) and VLDL-associated remnant particles. In vitro studies have suggested that VLDL induces foam cells formation. To reveal the changes of the proteins expression in the process of foam cells formation induced by VLDL, we performed a proteomic analysis of the foam cells based on the stimulation of differentiated THP-1 cells with VLDL. Using two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser-desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis, 14 differentially expressed proteins, containing 8 up-regulated proteins and 6 down-regulated proteins were identified. The proteins are involved in energy metabolism, oxidative stress, cell growth, differentiation and apoptosis, such as adipose differentiation-related protein (ADRP), enolase, S100A11, heat shock protein 27 and so on. In addition, the expression of some selected proteins was confirmed by Western blot and RT-PCR analysis. The results suggest that VLDL not only induces lipid accumulation, but also brings about foam cells diverse characteristics by altering the expression of various proteins.

• Asian Pacific Journal of Cancer Prevention
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• 제14권1호
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• pp.367-372
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• 2013

Effects of the Epstein-Barr virus (EBV) on cellular protein expression are essential for viral pathogenesis. To characterize the cellular response to EBV infection, differential proteomes of gastric epithelial AGS cells were analyzed with two-dimensional gel electrophoresis (2-DE) followed by matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) and liquid chromatography electrospray/ionization ion trap (LC-ESI-IT) mass spectrometry identification. Mass spectrometry identified 9 altered cellular proteins, including 5 up-regulated and 4 down-regulated proteins after EBV infection. Notably 2-DE analysis revealed that EBV infection induced increased expression of heat shock cognate 71 kDa protein, actin cytoplasmic 1, pyridoxine-5'-phosphate oxidase, caspase 9, and t-complex protein 1 subunit alpha. In addition, EBV infection considerably suppressed those cellular proteins of zinc finger protein 2, cyclin-dependent kinase 2, macrophage-capping protein, and growth/differentiation factor 11. Furthermore, the differential expressional levels of partial proteins (cyclin-dependent kinase 2 and caspase 9) were confirmed by Western blot analysis.Thus, this work effectively provided useful protein-related information to facilitate further investigation of the mechanisms underlying EBV infection and pathogenesis.