• Journal of Periodontal and Implant Science
• /
• v.52 no.3
• /
• pp.242-257
• /
• 2022

Purpose: This study investigated periodontal ligament (PDL) restoration in osseointegrated implants using stem cells. Methods: Commercial pure titanium and zirconium oxide (zirconia) were coated with beta-tricalcium phosphate (β-TCP) using a long-pulse Nd:YAG laser (1,064 nm). Isolated bone marrow mesenchymal cells (BMMSCs) from rabbit tibia and femur, isolated PDL stem cells (PDLSCs) from the lower right incisor, and co-cultured BMMSCs and PDLSCs were tested for periostin markers using an immunofluorescent assay. Implants with 3D-engineered tissue were implanted into the lower right central incisors after extraction from rabbits. Forty implants (Ti or zirconia) were subdivided according to the duration of implantation (healing period: 45 or 90 days). Each subgroup (20 implants) was subdivided into 4 groups (without cells, PDLSC sheets, BMMSC sheets, and co-culture cell sheets). All groups underwent histological testing involving haematoxylin and eosin staining and immunohistochemistry, stereoscopic analysis to measure the PDL width, and field emission scanning electron microscopy (FESEM). The natural lower central incisors were used as controls. Results: The BMMSCs co-cultured with PDLSCs generated a well-formed PDL tissue that exhibited positive periostin expression. Histological analysis showed that the implantation of coated (Ti and zirconia) dental implants without a cell sheet resulted in a well-osseointegrated implant at both healing intervals, which was confirmed with FESEM analysis and negative periostin expression. The mesenchymal tissue structured from PDLSCs only or co-cultured (BMMSCs and PDLSCs) could form a natural periodontal tissue with no significant difference between Ti and zirconia implants, consequently forming a biohybrid dental implant. Green fluorescence for periostin was clearly detected around the biohybrid implants after 45 and 90 days. FESEM showed the invasion of PDL-like fibres perpendicular to the cementum of the bio-hybrid implants. Conclusions: β-TCP-coated (Ti and zirconia) implants generated periodontal tissue and formed biohybrid implants when mesenchymal-tissue-layered cell sheets were isolated from PDLSCs alone or co-cultured BMMSCs and PDLSCs.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
• /
• v.24 no.12
• /
• pp.982-987
• /
• 2011

In this study, $Ge_8Se_{(2+x)}Te_{(6-x)}$ thin film amorphous-to-crystalline phase-change rate was evaluated in using a nano-pulse scanner. The focused laser beam with a diameter <10 ${\mu}m$ was illuminated in the power (P) and pulse duration (t) ranges of 1-31 mW and 10-460 ns, respectively, with subsequent detection of the responsive signals reflected from the film surface. We also evaluated the material characteristics, such as optical absorption and energy gap, crystalline phases, and sheet resistance of as-deposited and annealed films. The result of experiments showed that the thermal stability of the Ge-Se-Te film is largely improved by adding Se.

• Proceedings of the KSME Conference
• /
• 2001.06d
• /
• pp.121-126
• /
• 2001

In this study, reduced-scale experiments were conducted to understand smoke movements in tunnel fires with the natural ventilation. The 1/20 scale experiments were conducted under the Froude scaling since the smoke movement in tunnels is governed by buoyancy force. Six cases of experiments(pool diameter is 6.5cm, 7.3cm, 8.3cm, 10cm, l2.5cm and l5.4cm), in which vertical vents positioned 1m from the fire source symmetrically, were conducted in order to evaluate the effect of the vent on smoke movement. In case of heat release rate under 2MW, smoke front reached to the tunnel exit about 20 see delayed with ventilation and the smoke velocity was proportional to the power of the heat release rate. Temperature after the vent was lower than without vent. In case of l5.4cm pool, the temperature difference was about $50^{\circ}C$. It was confirmed that the thickness of smoke layer was maintained uniformly under the 35% height of tunnel through the visualized smoke flow by a laser sheet and the digital camcoder.

• Transactions of the Korean Society of Automotive Engineers
• /
• v.7 no.9
• /
• pp.63-74
• /
• 1999

When a fuel was injected with opening the intake valve of a port fuel injection engine, the spray atomization and flow characteristics in the intake port have a strong influence on the mixture formation of a combustion chamber. Thus , this study was to clarify the spray flow characteristics of the air-assist gasoline spray with fine dropkets across the suction air stream in model intake port. For the simulated opening intake valve in port, suction air stream was varied to 10m/s ∼30m/s. And fuel pressur ewas fixed to 300kPa, but air assist pressure was varied to 0∼25kPa for a vairable spray conditions. Spray flow trajectory was investigated by means of laser sheet visualization and the measurements of droplet sizes and velocities were made by PDPA system. Measured droplets within the spray flow field were subdivided into five size groups and then, the flow characteristics of droplet size groups were investigated to the spray across a suction air stream.

• Proceedings of the Korean Society for Technology of Plasticity Conference
• /
• 2003.05a
• /
• pp.434-438
• /
• 2003

The shearing process for the sheet metal is normally used in the precision elements such as semi-conductor components. In these precision elements, the burr formation brings a bad effect on the system assembly and demands the additional de-burring process. In this paper, we have developed the desktop-type precision punching system to investigate the burr formation mechanism and present kinematically Punch-die auto aligning methodology, for the purpose of burr unifomizing and minimizing, between the rectangular shaped punch and die. By using the scanning electron microscope, the aligned punching results are compared with the miss-aligned ones. Also, we measured the relative burr heights using the self-designed laser measuring device for insitu self aligning. Since it is hard to get the perfect, so called, burr-free edges during the shearing process, we introduced the ultrasonic do-burring machine. The de-burring operation was carried out by a novel do-burring method, the reversal flow resistance method, under different machining loads and abrasive types. The final do-burring results show the validity of our punching do-burring system pursuing the burr-free punched elements.

• Transactions of Materials Processing
• /
• v.24 no.4
• /
• pp.256-263
• /
• 2015

Electric resistance spot welding has been used to join overlapped steel sheets in automotive bodies. Recently to reduce weight in automotive vehicles, non-ferrous metals are being used or considered in car bodies for hoods, trunk lids, doors parts, etc. Various welding processes such as laser welding, self-piercing rivet, friction stir welding are being used. In the current study, a new electric resistance heated friction stir spot welding is suggested for the spot welding of non-ferrous metals. The welding method can be characterized by three uses of heat -- electric resistance heating, friction stir heating and conduction heating of steel electrodes -- for the fusion joining at the interfacial zone between the two sheets. The welding process has variables such as welding current, diameter of the steel electrodes, revolutions per minute (rpm) of the friction stir pin, and the insert depth of the stir pin. In order to obtain the optimal welding variables, which provide the best welding strength, many experiments were conducted. From the experiments, it was found that the welding strength could be reached to the required production value by using an electrode diameter of 10mm, a current of 7.6kA, a stirring speed of 400rpm, and an insert depth of 0.8mm for the electric resistance heated friction stir spot welding of 5052 aluminum 1.5mm sheets.

• 한국가시화정보학회:학술대회논문집
• /
• 2005.12a
• /
• pp.64-68
• /
• 2005

In this study, the smoke flows of the inner subway station were visualized through a numerical analysis and visualization experiment in the subway fire. A transparent acrylic model was designed and installed as 1:25th scale-down as the actual subway station by using geometrical similarity The properties of subway fire were reconstructed according to Densimetric Froude Similarity. The 47 to 53 ratio of the mixed air and Helium was inputted in the inner acrylic model to describe 1MW fire intensity with reference to the experiment paper. For the same time, the fire smoke from a smoke generator was inputted in the inner acrylic model with the mixture. At this time, the buoyancy effect of Helium gas went up the smoke to the acrylic model. When the sheet beam of Ar-lon laser was given out to the top and stair of subway model, the digital camcorder took the images of the scattered cluster of smoke particles when applying the smoke management system and PSD.

• Transactions of the Korean Society of Mechanical Engineers B
• /
• v.27 no.11
• /
• pp.1637-1644
• /
• 2003

Stereoscopic particle image velocimetry is a measurement technique to acquire three dimensional velocity field by two cameras. With a laser sheet illumination, the third velocity component can be deduced from out-of$.$plane velocity components using a stereoscopic matching method. Most industrial fluid flows are three dimensional turbulent flows, so it is necessary to use the stereoscopic PIV measurement method. However the existing stereoscopic PIV system seems hard to use since it is very expensive and complex. In this study we have developed a Miniature Stereo-PIV(MSPIV) system based on the concept of the Miniature PIV system which we have already developed. In this paper, we address the design and some primitive experimental results of the Miniature Stereo-PIV system. The Miniature Stereo-PIV system features relatively modest performances, but is considerably smaller, cheaper and easy to handle. The proposed Miniature Stereo-PIV system uses two one-chip-only CMOS cameras with digital output. Only two other chips are needed, one for a buffer memory and one for an interfacing logic that controls the system. Images are transferred to a personal computer (PC) via its standard parallel port. No extra hardware is required (in particular, no frame grabber board is needed).

• International Journal of Automotive Technology
• /
• v.5 no.3
• /
• pp.155-164
• /
• 2004

The direct injection gasoline spray-wall interaction was characterized inside a heated pressurized chamber using various visualization techniques, including high-speed laser-sheet macroscopic and microscopic movies up to 25,000 frames per second, shadowgraph, and double-spark particle image velocimetry. Two hollow cone high-pressure swirl injectors having different cone angles were used to inject gasoline onto a heated plate at two different impingement angles. Based on the visualization results, the overall transient spray impingement structure, fuel film formation, and preliminary droplet size and velocity were analyzed. The results show that upward spray vortex inside the spray is more obvious at elevated temperature condition, particularly for the wide-cone-angle injector, due to the vaporization of small droplets and decreased air density. Film build-up on the surface is clearly observed at both ambient and elevated temperature, especially for narrow cone spray. Vapor phase appears at both ambient and elevated temperature conditions, particularly in the toroidal vortex and impingement plume. More rapid impingement and faster horizontal spread after impingement are observed for elevated temperature conditions. Droplet rebounding and film break-up are clearly observed. Post-impingement droplets are significantly smaller than pre-impingement droplets with a more horizontal velocity component regardless of the wall temperature and impingement angle condition.

• Journal of the Korean Society for Marine Environment & Energy
• /
• v.1 no.2
• /
• pp.40-51
• /
• 1998

The interaction of contained oil slicks with current was investigated with a two-dimensional experimental setup in the circulating water channel facility. A vertical plate was used to contain the oils against the currents and the evolution of the oil slick, mainly focusing on the water/oil interface, was examined with an aid of a laser sheet. Two different oils - soy bean oil and diesel oil - were studied with varying the current speed (10 cm/sec to 35 cm/sec), the barrier depth (4 cm and 8 cm) and the volume of oil (2 liter to 12 liter). Different types of the interface behavior were observed according to the conditions and their mechanism was discussed based basically on the dimensional analysis. The critical speeds of two types of oil loss mechanism (entrainment failure and drainage failure) were also examined.