• Title/Summary/Keyword: Larval stage

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Pathogenicity of Entomopathogenic Nematode, Steinernema carpocapsae against Fall Webworm, Hyphanria cunea (Lepidoptera: Arctiidae) (미국흰불나방(Hyphanria cunea)에 대한 곤충병원성선충 Steinernema carpocapsae의 병원성)

  • Park Hyung Soon;Kim Hyeong Hwan;Chung Hun Gwan;Cho Yoon Sin;Jeon Heung Yong;Jang Han Ik;Kim Dong Soo;Choo Ho Yul
    • Asian Journal of Turfgrass Science
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    • v.18 no.4
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    • pp.193-200
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    • 2004
  • Environmentally sound control of fall webworm, Hyphanria cunea (Drury) with entomopathogenic nematode, Sreinernema carpocapsae Pocheon strain was evaluated in the laboratory and pot. Pathogenicity of 5. carpocapsae Pocheon strain was different depending on larval stage, i.e., mortality of the 2nd instar and the $3\~4th$ instar was $100\%$ with >20 infective juveniles (Ijs)/larva in 3 days, but the 5th instar was $34\%$ with (Ijs)/larva in 3 days. Pathogenicity of 5. carpocapsae Pocheon strain was higher with increasing nematode concentration. Mortality of Hyphanria cunea larva by 5. carpocapsae Pocheon strain was not significantly different (more than $70\%$) between nematode concentration on treated trees (Malus alba and Platanus orientalis) and in pot.

Vertical Distribution Characteristics of Snow Crab Chionoecetes spp. Larvae in the East Sea (한국 동해에 서식하는 대게류(Chionoecetes spp.) 유생의 수직 분포 특성)

  • Hyeon Gyu Lee;Bo Ram Lee;Jeong-Hoon Lee;Seung Jong Lee;Hwan-Sung Ji
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.56 no.2
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    • pp.221-227
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    • 2023
  • The vertical distribution of snow crab Chionoecetes spp. larvae in the East Sea were investigated in April 2021 using the Multiple Opening/Closing Net and Environmental Sensing System (MOCNESS). The water temperature ranged from 0.86 to 17.2℃, and salinity from 34.0 to 34.7 psu. Zoea I and II occurred range from 29 to 1,982 inds.103 m-3 at 10 stations, and range from 4 to 11 inds.103 m-3 at 3 stations, separately. Therefore, Zoea I occurred in wider area and higher density than Zoea II at all stations. In the vertical distribution of larvae, all zoeal stages were mainly distributed in the 30-40 m strata, and the larvae showed nocturnal vertical migration similar to that of most zooplankton. Zoea I appeared in the range from 13.7 to 15.8℃ and occurred at the highest density of 1982 inds.103 m-3 at 14℃. And Zoea II appeared range from 13.4 to 14.5℃ and occurred in the highest density of 11 inds.103 m-3 at 13.4℃. In conclusion, the early larval stage (zoea I) occurred at a higher range of sea surface temperature than later larval stage (zoea II).

Effect of temperature on growth and oviposition characteristics of Meganoton scribae (Lepidoptera: Sphingidae)

  • Kim, Seong-Hyun;Park, Haechul;Park, Ingyun
    • International Journal of Industrial Entomology and Biomaterials
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    • v.33 no.2
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    • pp.63-67
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    • 2016
  • This study examined the biological characteristics of Meganoton scribae under the following conditions: temperature (20, 25, $30^{\circ}C$), humidity ($60{\pm}10%$), and photoperiod (14L:10D). At $25^{\circ}C$, the larval stage of M. scribae lasted for 28.4 d, and the pupal stage for 15.6 d. The average head width was 0.96 mm in the $1^{st}$, 1.34 mm in the $2^{nd}$, 2.07 mm in the $3^{rd}$, 3.64 mm in the $4^{th}$, and 5.89 mm in the $5^{th}$ instar. The life span of female adults was 10.3 d, and the maximum number of eggs spawned was 257.7. The results of the study will be useful for the commercial development of M. scribae.

The Expression of Corazonin Neurons in Larvae Stage of Scuttle Fly

  • Park, Hohyun
    • Biomedical Science Letters
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    • v.26 no.3
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    • pp.217-225
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    • 2020
  • Scuttle fly which moves abruptly after standing for a while and stop suddenly to rush off again, is a fly species in the Phoridae family. This species like rotten organic materials and it is known to proliferate even in the industrial materials including organic solvents. These characteristic behaviors of the scuttle fly seem to be related to muscular and nervous system or neurotransmitters. Thus, we focused at the neurotransmitter, corazonin (Crz) that is known to be related to resistance to stress and investigated the developmental process of the neurons in the scuttle fly. Corazonin is a neuropeptide being expressed in the central nervous system (CNS) and is known to control mainly physiological functions and behaviors. Its many functions that have been proposed are still in controversy. In this studies, we found that there are three groups of corazoninergic neurons in the larval CNS of the scuttle fly and these neurons undergo distinguishable changes through metamorphic process compared to different fly species. Larva has 3 pairs of Crz neurons at the dorsolateral area of the brain, 1 pair at the dorsomedial brain and 8 pairs at the ventral nerve cord.

Development of the Short-Horned Grasshoppers, Oxya sinuosa, at the Constant Temperature (恒溫條件下에서의 우리벼메뚜기 ( Oxya sinuosa ) 의 發育)

  • Joo, Eun-Young
    • The Korean Journal of Ecology
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    • v.14 no.1
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    • pp.9-13
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    • 1991
  • The short-horned grasshoppers, Oxya sinuosa Mistshenko, were reared in the growth cabinet exposed alternately to 16 hours of artificial light (510${\pm}$240 lux) and 8 hours of darkness under the condition of constant temperature of 30${\circ}C$ and 65${\pm}$3% in relative humidity. The results obtained in this study are summarized as follows. Both female and male grasshoppers had seven instars. In the larval developmental stages of female and male, the third stage was shorter than the other stages, and the longest stage was the seventh. The total developmental period of females and males were approximately 80.17 and 63.5 days, respectively. The survivorship curve at the temperature of 30${\circ}C$ shows straight line.

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A Note on the Deveolopment of Tribolium castaneum (Coleoptera: Tenebrionidae) on Brown Rice Oryzae sativa L. (현미에서의 거짓쌀도둑, Tribilium castaneum(딱정발레목:거저리과)의 발육)

  • 전환구;홍영석;류문일
    • Korean journal of applied entomology
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    • v.30 no.2
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    • pp.103-137
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    • 1991
  • The developmental period(mean $\pm$ SE) of the red flour beetle, Tribolium castaneum was studied on brown rice at four constant temperature(25, 28, 34, $36^{\circ}C$ $\pm$ $0.5^{\circ}C$, RH 70%)conditions. Based on the results, the lower and the upper developmental threshold temperature for the beetle were also estimated. The duration of the egg stage was estimated to be 7.6 $\pm$ 0.25, 4.8 $\pm$ 0.10, 3.0 $\pm$ 0.03 and 2.5 $\pm$ 0.09 days at 25, 28, 34, $36^{\circ}C$, respectively. That of the larval stage was estimated to be 53.3 $\pm$ 1.49, 33.4 $\pm$ 0.57, 30.6 $\pm$ 0.70, 31.0 $\pm$ 1.18; that of the pupal stage to be 12.1 $\pm$ 0.17, 7.8 $\pm$ 0.09, 5.7 $\pm$ 0.09, 5.0 $\pm$ 0.07 ; that of the overall period which spans from eggs to the adult emergence to be 72.3 $\pm$ 1.67, 46.0 $\pm$ 0.55, 39.4 $\pm$ 0.64, 38.7 $\pm$ 1.15 days at 25, 28, 34, $36^{\circ}C$, respectively. The duration of the egg and the pupal stage were similar to those of the results reared on other diets such as wheat and maize. Fed on brown rice, however, the duration of the larval stage was significantly retarded than on wheat. The mortality of the beetle during ,development was higher on brown rice than on wheat reported, indicating that brown rice is a poor diet compared with wheat. The lower developmental threshold temperature was estimated to be $20.0^{\circ}C$ for all stages of the beetle. The upper one was estimated to be $40.2^{\circ}C$ for the overall stage. The modal value of the moulting times during the larval stage was 7. However, as temperature increased,.the frequency of the beetle that moulted more than 7 times became higher. The sex ratio of the beetle was not affected by temperature.

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REARING OF THE LARVAL PRAWN, PENAEUS JAPONICUS BATE (보리새우 Penaeus japonicus Bate의 유생사육에 관하여)

  • PYEN Choong-Kyu
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.2 no.1
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    • pp.87-91
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    • 1969
  • Experiments on the rearing of larvae of the prawn, Penaeus japonicus Bate, have been con-ducted by using a large tank A ($3.4\times1.9\times1.0m$) and two small tanks B and C ($1.45\times0.85\times1.0$). 1) Between spawning and the first zoeal stages, no significant elapsed time difference was noticed among the rearing tanks. At about $23^{\circ}C$ of water temperature nearly all of the larvae in the tanks metamorphosed into the first zoeal stage in about 36-48 hours. However the period of time which elapsed between the spawning and post-larval stage showed some differences bet-ween the tanks, i.e., 19-20 days in tank A and 15-17 days in tanks B and C, respectively 2) No difference in body length of the larvae has been observed among the three tanks. 3) The post-larva passed through several molts, one every four or five days, before reaching the young prawn about 36-40 days after spawning. 4) Throughout the zoeal stages the highest mortality was found at the time of molting between the first and second zoeal stages showing about $51.39\%$ in tank A, $50.70\%\;and\;31.91\%$ in tanks B and C, respectively. 5) Total mortality during the duration of the larval stages was around $75\%$ in all the rearing tanks.

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Effect on Early Life Stage of Three Freshwater Fish (Carassius auratus, Cyprinus carpio, Oryzias latipes) Exposed to Suspended Solids (부유토사가 담수 어류 3종(붕어, 잉어, 송사리)의 초기 생활사에 미치는 영향)

  • Moon, Seong-Dae;Kang, Sin-Kil;Lee, Chang-Hoon;Sung, Chan-Gyoung;An, Kwang-Guk;Choi, Tae Seob
    • Korean Journal of Ecology and Environment
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    • v.47 no.2
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    • pp.82-90
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    • 2014
  • To assess the adverse effects of suspended solids on fishes, the hatching rate of embryo, and survival and growth of larvae were measured with common fish species of river such as crusian carp (Carassius auratus), common carp (Cyprinus carpio) and medaka (Oryzias latipes). Bioassay on hatching rate of embryo was conducted with a range of turbidity as 10 levels from 0.2 to 8,080 NTU. Another bioassay on 4-day larval survival and 21-day larval growth were also conducted with a range of turbidity as 8 levels from 0.1 to 8,260 NTU, and 6 levels from 0.7 to 2,030 NTU, respectively. The hatching rate of C. auratus was not significantly different from that of control at turbidity below 4,040 NTU, whereas it decreased when turbidity was 8,080 NTU (p<0.05). The hatching rate of C. carpio was not affected by concentration of suspended solids. For O. latipes, the hatching rate significantly decreased from 254 NTU (p<0.001) and it was zero when turbidity was 8,080 NTU. The 4-day survival of larvae of all 3 species was not affected by turbidity, while the larval growth of all 3 species was significantly affected. The turbidity effect on the growth of C. auratus and C. carpio was observed from 7 days after the exposure at turbidity level of 145, 143 NTU, respectively. The turbidity effect on the growth of O. latipes was observed from 14 days after the exposure at turbidity level of 254 NTU. The results of this study will provide the basic information for the derivation of water quality criteria on suspended solids for the protection of aquatic ecosystem and the quantitative ecological risk assessment of freshwater environment.

Ecological Characteristics of Mulberry Longicorn Beetle, Apriona germari Hope, at the Hibernation Stage in Mulberry Fields (뽕밭에서 월동하는 뽕나무하늘소(Apriona germari Hope)의 생태적 특성)

  • 윤형주;박인균;마영일;이상범;양성열
    • Korean journal of applied entomology
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    • v.36 no.1
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    • pp.67-72
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    • 1997
  • To study the ecological characteristics of the mulberry longicorn beetle, Apriona germari Hope, Mulberry branches with egg-laid scar were regionally collected from ten mulberry fields during the hibernation. It showed that its distribution was highest in Kyunggi province with 3.05% and lowest in Kyungnam province with 0.28%. The number of the egg-laid scar per branch was single with 87.7% of high frequency and its average size was 2.38 L$\times$1.45 W$\times$0.34D cm. The average diameter of scarred branch was 1.7 cm, ranging from 1.3 to 1.9 cm. the shape of egg was long elliptical with width of $2.32\pm$0.33 mm and with length of $6.606.60\pm$0.55 mm. the weight of egg was $16.6\pm$2.94 mg and the color of the just-oviposited egg was milky white, changing into thick brown with time. The weight and length of the just-hatched larva was $16.6\pm$2.4 mg and $6.36\pm$0.66 mm. respectively. Most of the larvae moved downward inside the branch, showing that 94.2% of larvae for outdoor rearing and 87.9% of larvae for indoor rearing moved downward in the branch. As a result, it was found that it hibernated at egg stage with a higher rate than at larval stage by two or three folds in Kyunggi and Kyungbuk province and contrarily at larval stage with higher rate than at egg stage in Chunbuk, Chunnam and Kyungnam provinces. The survival rate for the egg after hibernation was significantly low with 6.6% and contrarily for the larva after hibernation remarkably high with 91.2%. Although the survival rate of the egg in this survey was relatively low, the result indicated that there were two types of hibernation of A. germari in Korea; egg and larval stage.

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Ontogeny of the Digestive Organ during Early Life Stages of the Black Sea Bream, Acanthopagrus schlegeli(Teleostei: Sparidae) (감성돔(Acanthopagrus schiegeli)의 초기생활사 동안 소화기관 발달)

  • LEE Jung Sick;KIM Heung-Yun;BYUN Soon Gyu;KIM Jin Do;GO Chang Soon;CHIN Pyung
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.33 no.2
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    • pp.129-136
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    • 2000
  • Differentiation and development of the digestive organ of the black sea bream, AcanHepagus schlegeli were studied by means of histological methods. The hatched lawn if TL(total length) $2.0 mm (n=10)$ had a yolk sac of $1,000{\times}590 {\mu}m$ and simple straight digestive tract, which was composed of cuboidal epithelium. In the pre-larval stage of TL $3.5 mm$, digestive tract could be distinguished into esophagus, stomach and intestine, and the exocrine glands were appeared in the pancreas. In this stage mucosal folds, eosinophilic granule cells and brush border were observed in the posterior intestine. Yolky materials were completely absorbed and the brush border was recognized in the free surface of anterior intestine in TL $3.7 mm$. In the stomach mucosal folds began to appear from TL $4.0 mm$. In this time the zymogen granules were recognized in the cytoplasm of pancreatic exocrine cells. In the post-larval stage ranged from $4.5 to 5.0 mm$ in TL, hepatic cords started to develop, and the mucous secretory cells of PAS positivewere observed at esophagus and intestine. In the post-larval stage ranged from $6.3 to 7.0 mm$ in TL, histological layer of esophagus and intestine could be distinguished into serous membrane, muscular layer, submucosal layer and mucosal layer. From over TL $9.0 mm$, stomach could be distinguished into cardiac, fundic and pyloric portion, and the gastric gland began to appear at mucosal fold of fundic stomach. In the juvenile stage ranged from $10.0 to 11.0 mm$ in TL, histological structures of esophagus and intestine were similar to those of adult. From over TL $15.0 mm$, histological structures of stomach were similar to those of adult. Structural and functional digestive organ of black sea bream was present from the juvenile stage ranged from $15.0 to 17.0 mm$ in TL.

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