• Journal of Life Science
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• v.27 no.6
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• pp.702-707
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• 2017

Insects and their extract have been traditionally used as medicinal resources in Asia. Recently, Gryllus bimaculatus and the larva of Tenebrio molitor, Protaetia brevitarsis, and Allomyrina dichotoma were enlisted as general food ingredients by Korean Ministry of Food and Drug Safety. As the interest in these insects is growing, the detailed investigation is needed for the standardization of suitable and safe rearing techniques. This survey was conducted via personal interviews about edible insect rearing farms in Korea. The basic feed is fermented oak sawdust for P. brevitarsis and A. dichotoma and wheat bran for T. molitor. The various feed additives are blended with each farm's own recipes. The overall growth condition, including sawdust fermentation method, feeding period, and rearing density, varies among the investigated farms. It seems that weight of last instar larva was correlated with the rearing density and duration of the larval period. The heavy metal contents were analyzed after 48 hour starvation and they were very small amount or not detected in investigated insects. We believe that the present data will contribute to develop and standardize the safe rearing techniques of edible insects in Korea.

• Korean journal of applied entomology
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• v.12 no.1
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• pp.41-46
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• 1973

1) The optimum temperature for mass rearing of stable fly was $26^{\circ}C$ centigrade. Number of days required for stage of development at $26^{\circ}C$ were 6.8 days for larval stage, 5.3 days for pupla stage, 10.4 days for preovipositionla stage, and 30 days for adult stage respectively. 3) The pupation rate, emergence rate and sex ratio were $80.7\%,\;84.3\%$ and 1 : 1, respectively. 3) The average weight of pupae was 14.5mg, and the standard medium showed better result in larvae rearing than wheat bran medium. 4) The optimum number of eggs for inoculation on 125gr medium was approximately 310. 5) Optimum size of resting place was determined as $2inch^2/adult$ when it reared in a rectangular cage.

• Korean journal of applied entomology
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• v.48 no.2
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• pp.269-274
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• 2009

Rearing media were selected for the production of sterile larvae of greenbottle blowfly, Phaenicia (=Lucilia) sericata (Meigen) which is widely used in maggot therapy. Eight media available in the market were used in this study. Egg hatchability was not different among the media. Survivorship of the larvae was higher in blood agar (BA), sabouraud dextrose agar, and brucella blood agar than the others. A higher content (20${\sim}$40%) of sheep blood in BA and chocolate agar increased the survivorship of larvae. The eggs and the early 3rd larvae could be stored for 12 days at $8^{\circ}C$ and for 15 days at $6^{\circ}C$ without decrease in hatchability and larval survivorship, respectively.

• Journal of fish pathology
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• v.16 no.1
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• pp.13-21
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• 2003

The infection characteristics with scuticociliates at on-land rearing farms and hatcheries of flounder, Paralithys olivaceus was investigated during the year of 2001 by juvenile infection routes. When culture tanks for living food organisms such as chlorella, rotifer, and Artemia were searched, scuticocilates were detected both in live and dead rotifer, and at the dregs of culture tank bottoms at almost hatcheries. When rotifer infected with scuticocilates fed on fish larvae, lots of scuticocilate were inhabited at the bottom of fry rearing tanks. After feeding on scuticocilates-infected rotifer on fish larvae, first infection was detected at 10 days after bottom dwelling or 40 days old after hatching. By histopathological examination we confirmed the infection route of eyeball or brain contamination was that the ciliate worms digged through mouth and front part of the dosal fin cuticle, transferred into eyeball along the epithelium and muscle tissue, and reached finally into brain by the muscle and nerve tissue. The infection of internal organs was clarified into two routes. The first route was started from the infection at ventral and anal fin rays by the worms, and reached at the anus and rectum through the epithelium and muscle tissue. The second route was initiated from the infection at urinary organ and reached into the rectum epithelium cells, inner wall of intestine, abdominal cavity, pancreas, kidney, and pancreas. At seed production farms where fish larvae fed on scuticocilate-free rotifer, the worms were not detected not only at the food organisms culture tanks and juvenile rearing tanks but also larval flounder less than 7cm in total length.

• Journal of Sericultural and Entomological Science
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• v.50 no.2
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• pp.133-139
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• 2012

The Aims of this study are to investigate ecological environments and indoor-rearing conditions of Eurema hecabe for artificial rearing. Two different nesting site, a open area nearby small river(A area; Daejin-ri, Gonyang-myeon, Sachun-si, Gyoungsangnam-do) and a foot of a hill area(B area; Noryang-ri, Seolcheon-myeon, Namhae-gun, Kyoungsangnam-do), selected for investigation of ecological habitat environment, and investigated two times per month, during six month, from June to November in 2010. In result, the number of butterflies, Eurema hecabe, at the hill area(B area) was 6 times more than the open area(A area). We observed the number of eggs, larvae and puapae in the B area more times compared to those in the A area. In the B area, Lespedeza cuneata was higher preference than the other host plant, Lespedeza pilosa and Albizia julibrissin. For development of indoor-rearing conditions, common grass yellow butterfly, Eurema hecabe, was reared in a room condition. As a result of oviposition preference experiment, 100-mated-female laid significantly higher number of eggs $104.9{\pm}19.6$ on Lespedeza cuneata plant, whereas the number of eggs was $12.7{\pm}4.5$ on Cassia obtusifolia plant. Moreover, the eggs hatched within $5.1{\pm}0.9$ days from the day of oviposition and procent of egg hatchability was 53.7% on host plant. The headwidth of each developmental larval stage were $0.36{\pm}0.02$(1st), $0.61{\pm}0.02$(2nd), $0.93{\pm}0.05$(3rd), $1.46{\pm}0.08$(4th), $2.25{\pm}0.11$(5th). The larval period was $12.1{\pm}0.9$ days under high temperature, long day condition($25^{\circ}C$, 16L : 8D), showing 81.0% pupal ratio. The pupal period was $6.9{\pm}0.7$ days, and the emergence rate was 79.6%. Based on above experiment, artificial rearing system of common grass yellow butterfly, Eurema hecabe was completed in indoor condition.

• The Korean Journal of Malacology
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• v.28 no.2
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• pp.145-156
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• 2012

Larval development of the Manila clam Ruditapes philippinarum reared in an indoor tank system was examined in this study using light microscope and scanning electron microscope. To induce spawning and subsequent larval development, clams were collected from the intertidal zone at Gim-nyeong harbor in Jeju Island in August 2011. After 2 days of rearing in the tank, all Manila clams spawned in the midnight. Non-feeding trochophore larvae appeared 7hrsafter fertilization and the first D-shape larvae could be observed at 19 hrs. Twenty one days after fertilization the pediveliger larvae crawling on the bottom of the tank with well-developed foot were observed. Histology indicated that all the clams used in this study were in the ripe stage prior to spawning and the gonad-somatic index (GSI), a ratio of the egg mass to the tissue weight, of the ripe female measured by ELISA was 28.6%. The GSI of female clam declined to 17.3% after the massive spawning in the tank, suggesting that Manila clam discharged 40% of the total eggs during the first spawning event. In conclusion, spawning and subsequent larval development of Manila clam was successfully carried out in this study using an indoor tank system, and the information obtained in the present study could be useful in future Manila clam hatchery development.

• Journal of Sericultural and Entomological Science
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• v.50 no.1
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• pp.27-32
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• 2012

The aim of this study is to develop indoor-rearing methods of the diving beetles. In nature, both the adult diving beetle and its larvae are voracious aquatic predators. The larvae beetles hunt relatively bigger size of tadpoles and small fish for food source. However, due to difficulties of the food supplement for rearing diving beetles at indoor-condition further motivated us to develop new artificial food. Three separate experiments were performed. In the first experiment, adult beetles were provided with one of the several food choice treatments to self-compose their preferred foods that are affordable on the market at lower price. The second experiment was also to develop artificial diet that is possible for rearing larvae beetle under indoor condition. The larvae beetles were restricted to raw squid, artificial food source and mosquito larvae as a control at the first stadium and small fish and raw squid during second to third stadium duration. According to our result, adult beetles selected a food that made of boiled squid and dead small fish while, the young larvae consumed small fish, mosquito larvae and raw squid. Although, the larval food restriction on law squid caused noticeable decrease in survival, the result still supported the possible survival rate of keeping larvae at indoor condition. Moreover, pupation rate experiments, in which groups of larvae were placed at different mats, natural soil and fermented sawdust, showed that 80% of diving beetles pupated on the sawdust. This result indicates that female beetle preferentially selected to oviposit along soft and moist area.

• Korean journal of applied entomology
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• v.58 no.1
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• pp.69-76
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• 2019

This study was evaluated to increase the production efficiency of Gryllus bimaculatus De Geer, which are recently increasing attention as industrial insects in Korea. In this study, the hatching rate and larval period were investigated along with temperature and humidity, as well as the survival rate and ovipositional temperatures along with rearing density. The optimum relative humidity for hatching was highest at 90%, which shows the hatching rate of 90%. The highest hatching rate was 98.3% at $20^{\circ}C$. The hatching period was shortest at $35^{\circ}C$ incubation, which shows 7.1 days in average. The survival rate at 3,000-20,000 individuals in the breeding container was 34-18% after rearing for 35 days. In the effect test of feeding vegetables, the survival rate was 1.8 times and the biomass weight 2.5 times higher than that of normal individuals respectively. The number of laying eggs by temperature was highest at $25^{\circ}C$, which shows 1,710 eggs after reading for 30 days.

• Journal of Sericultural and Entomological Science
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• v.17 no.2
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• pp.141-149
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• 1975

Silkworm is known to be damaged under the unfavorable environmental conditions. Recently, it has been often argued that nicotine is a main factor to affect the silkworm nutritional balance and to result in decrease of quantitative characters, and unbalance of larval nutrition. Especially in spring season, there is tendency to produce nicotine damage to silkworm fed with the mulberry leaf near to tobacco field early cultivated in mulching system. In these connections, the trial was made in the different pots placing by the different distance 10rn, 20m, 40m, and 60m in the direction of east-north from the tobacco field. Location of pot near to the tobacco fold is 40m below the tobacco Held and gradually higher than the tobacco field by 10 degree of slope. In addition, when the mulberry field is divided into two areas from the east-south to west-north, southern east zone is lowering down below 10 degree of slope and has 36％ of east-south wind, 21％ of south-east wind and 16％ of south wind, etc. Pots are left on the mulberry field from the completion of lower part leaf harvest to just before upper part leaf harvest is completed for 32 days. Then silkworm at 4th-5th stage was fed with the leaf grown during the period and larval characters are statistically analyzed. The results are summarized as follows： 1. 10-40m distant pot statistically at the same level has the longest larval duration and 60m distant pot is. not significant at 5％ level. Whole larval duration has same tendency as in the 5th larval duration. 2. Percentage of delayed molting larvae at the 5th stage is increased in 10m distant pot and there is no significance statistically in other pots, as compared with that of control. 3. It is not showed that there is no damage to mortality of the old larvae in 60m distant pot. 4. 10-20m distant pot produces a low pupation and others are at the same level statistically at 5％ significance, 5. It is showed that nicotine damage is occurred in single cocoon freight and cocoon shell weight up to 60m distant pot, rather higher damage in cocoon shell weight than in single cocoon weight. 6. It is showed that cocoon yield from 10, 000 molted larvae including double cocoon is decreased up to 40m distant pot far from the tobacco field in which 40％ of cocoon yield in 10m distant pot by 7kg, 26％ of cocoon yield in 20m distant pot by 3.9kg, 24％ of cocoon yield in 40m distant pot by 3.6kg, and 12％ of cocoon yield in 60m distant pot by 1.8kg, as compared with control. However, there is no significance statistically at 5％ level in 60m distant pot. With these above results, it is concluded that nicotine da mage could be occurred in cocoon yield and cocoon quality up to 60m distant far from the tobacco field.

• Journal of Aquaculture
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• v.14 no.1
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• pp.51-56
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• 2001

At 5$^{\circ}C$ incubation of the brooded eggs of the snow crab, Chionoecetes opilio lasted for 297 days; freshly hatched prezoea molted to become the first zoea in one hour. Length (from the tip of the rostral spines to the tip of the dorsal spines) of the first and second zoeae measured 4.8 and 6.4mm, respectively. Experimental rearing of the larvae at 5, 10, 15 and 2$0^{\circ}C$ indicated that the upper limit of thermal tolerance is 15$^{\circ}C$, as all the reared larvae succumbed at 2$0^{\circ}C$. Intermolt period from the first the first zoea to the second was 57, 32 and 23 days at 5, 10 and 15$^{\circ}C$, respectively and that of the second zoea was 52, 29 and 90 days, respectively. Largest number of larvae survived at 1$0^{\circ}C$.