The Korean Journal of Malacology (한국패류학회지)

The Malacological Society of Korea (한국패류학회)
권호 표지
DOI QR코드

DOI QR Code

Quantification of Reproductive Effort and Microscopic Observation on the Larval Development of Manila clam Ruditapes philippinarum (Adams and Reeve, 1850)

바지락 (Ruditapes philippinarum)의 번식량 측정 및 유생발달에 관한 연구

  • Lee, Hee-Jung (School of Marine Biomedical Science, Jeju National University) ;
  • Kang, Hyun-Sil (School of Marine Biomedical Science, Jeju National University) ;
  • Park, Kyung-Il (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University) ;
  • Mondol, Mostafisur Rahman (School of Marine Biomedical Science, Jeju National University) ;
  • Choi, Kwang-Sik (School of Marine Biomedical Science, Jeju National University)
  • 이희중 (제주대학교 해양과학대학 해양생명과학과) ;
  • 강현실 (제주대학교 해양과학대학 해양생명과학과) ;
  • 박경일 (군산대학교 해양과학대학 수산생명의학과) ;
  • ;
  • 최광식 (제주대학교 해양과학대학 해양생명과학과)
  • Received : 2012.06.11
  • Accepted : 2012.06.22
  • Published : 2012.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Larval development of the Manila clam Ruditapes philippinarum reared in an indoor tank system was examined in this study using light microscope and scanning electron microscope. To induce spawning and subsequent larval development, clams were collected from the intertidal zone at Gim-nyeong harbor in Jeju Island in August 2011. After 2 days of rearing in the tank, all Manila clams spawned in the midnight. Non-feeding trochophore larvae appeared 7hrsafter fertilization and the first D-shape larvae could be observed at 19 hrs. Twenty one days after fertilization the pediveliger larvae crawling on the bottom of the tank with well-developed foot were observed. Histology indicated that all the clams used in this study were in the ripe stage prior to spawning and the gonad-somatic index (GSI), a ratio of the egg mass to the tissue weight, of the ripe female measured by ELISA was 28.6%. The GSI of female clam declined to 17.3% after the massive spawning in the tank, suggesting that Manila clam discharged 40% of the total eggs during the first spawning event. In conclusion, spawning and subsequent larval development of Manila clam was successfully carried out in this study using an indoor tank system, and the information obtained in the present study could be useful in future Manila clam hatchery development.

이 연구에서는 바지락 (Ruditapes philippinarum) 의 번식량 및 유생발생을 관찰하였으며, 이를 위하여 수정란 및 유생을 21일 간 실내사육수조를 이용하여 배양하였다. 바지락은 2011년 8월 제주시 김녕에서 채집 실험 수조로 옮겨졌으며, 다음날 자연적인 방란 방정이 관측되었다. 바지락 수정란은 19시간의 배 발생과정을 거쳐 21일 후 포복피면자유생(pediveliger) 으로 발달하였다. 조직학적 방법을 이용하여 바지락의 생식소를 관찰한 결과, 방란, 방정 전 모든 개체는 성적으로 완숙한 상태였고, 산란 후 모든 개체에서 부분산란이 관찰되었다. 효소면역학적 방법 (ELISA) 에 의한 번식량 측정 결과, 암컷 바지락의 생식소 지수는 (GSI) 산란 전 28.6%에서 산란 후 17.3%로 11.3% 감소하였으며, 추정된 포란수는 6,998,658 eggs였다. 이 연구서 광학현미경 및 전자현미경을 이용하여 관찰된 바지락 유생 발달 및 번식량은 향후 바지락 인공종묘 산업 및 바지락 자원관리에 있어 중요한 자료로 활용될 것으로 사료된다.

Keywords

References

  1. Affan, M. A. (2006) Ecological and ecophysiological studies and application of microalgae on the west coast of Jeju Island. Ph.D. Dissertation., Dept. Oceanography, Cheju National University. 115 pp.
  2. Chung, E.Y., Hur, S.B., Lee, J.S. (2001) Gonadal maturation and artificial spawning of the Manila clam Ruditapes philippinarum (Bivalvia: Veneridae), in Komso bay, Korea. journal of Fisheries Science Technology, 4: 208-218.
  3. Drummond, L., Mulcahy, M., Culloty, S. (2006) The reproductive biology of Manila clam, Ruditapes philippinarum, from the North-West of Ireland. Aquaculture, 254: 326-340. https://doi.org/10.1016/j.aquaculture.2005.10.052
  4. Hur, Y.B. (2005) Comparison of development and larval growth of four Venerid clams. Journal of the World Aquaculture Society, 36: 179-187.
  5. Healy, J.M. (1995) Comparative spermatozoal ultrastructure and its taxonomic and phylogenetic significance in the bivalve order Veneroida. In: Jamieson, B.G.M., Ausio, J., Justine, J.-L., (eds), Advances in spermatozoal phylogeny and taxonomy. Memoires Du Museum National D'Histoire Naturelle, 166: 155-166.
  6. Helm, M.M., Bourne, N. (2004) Hatchery operation: Broodstock conditioning, spawning, and fertilization. In: A. Lovatelli, editor. Hatchery culture of bivalve: A practical manual, FAO fisheries technical paper, Italy. 471 pp.
  7. Goulletquer, P. (1997) A bibliography of the Manila clam Tapes philippinarum. IFREMER, RIDRV-97.02/RA/La tremblade, 122p.
  8. Kang, C.K., Park, M.S., Lee, P.Y., Choi, W.J., Lee, W.C. (2000) Seasonal variation in condition, reproductive activity, and biochemical composition of Pacific oyster, Crassostrea gigas (Thunberg) in suspended culture in two coastal bays of Korea. Journal of Shellfish Research, 19: 771-778.
  9. Kang, C.K., Kang, Y.S., Choy, E.J., Kim, D.S., Shim, B.T., LEE, P.Y. (2007) Condition, reproductive activity, and gross biochemical composition of the Manila clam, Tapes Philippinarum in natural and newly created sandy habitats of the southern coast of Korea. Journal of Shellfish Research, 26: 401-412. https://doi.org/10.2983/0730-8000(2007)26[401:CRAAGB]2.0.CO;2
  10. Kang, D.H., Ngo, T.T.T., Koh, K.J., Choi, S.K. (2004) Seasonal changes in gonadal development of Manila clam, Ruditapes philippinarum form Shi-Heung-Ri, Jeju, Korea. Journal of Aquaculture 4: 81-88 (In Korean).
  11. Kang, D.H., Chu, F.E., Yang, H.S., Lee, C.H., Koh, H.B., Choi, K.S. (2010) Growth, reproductive condition, and digestive tubule atrophy of Pacific oyster Crassostrea gigas in Gamakman Bay off the southern coast of Korea. Journal of Shellfish Research, 29: 839-845. https://doi.org/10.2983/035.029.0418
  12. Kim, D.S., Lim, D.I., Jeon, S.K., Jung, H.S. (2005) Chemical characteristics and eutrophication in Cheonsu Bay, west coast of Korea. Ocean Polar Research, 27: 45-58. https://doi.org/10.4217/OPR.2005.27.1.045
  13. KOSIS. (2003) Statistic Database for Reclamation Extent.(http:/kosis.kr/wnsearch/totalSearch.jsp)
  14. Limpanont, Y., Yang, H.S., Park, K.I., Choi, K.S. (2011) First report on the annual gametogenesis of Heteromacomairus (Hanley, 1845) in a rocky intertidal area, northern Jeju Island, Korea. Journal of Shellfish Research, 30: 39-46. https://doi.org/10.2983/035.030.0107
  15. Min, D.K. (2004) Mollusks in Korea. Busan, Korea: Hangul Graphics. 465 pp.
  16. Ngo, T.T.T and Choi, K.S. (2004) Seasonal changes of Perkinsus and Cercaria infections in the Manila clam Ruditapes philippinarum from Jeju, Korea. Aquaculture 239: 57-68. https://doi.org/10.1016/j.aquaculture.2004.06.026
  17. NFRDI. 2001. Development of optimal technology for sustaining production in shellfish farm. National Fisheries and Development Institute of Korea technical report, 116 pp.
  18. Menegghetti, F., Moschino, V., Da Ros, L. 2004.Gametogenic cycle and variations in oocyte size of Tapes philippinarum from the Lagoon of Venice. Aquaculture, 240: 473-488. https://doi.org/10.1016/j.aquaculture.2004.04.011
  19. Park, K.I. and Choi, S.K. (2004) Application of enzyme-linked immunosorbent assay for studying of reproduction in the Manila clam Ruditapes philippinarum (Mollusca: Bivalvia) I. Quantifying eggs. Aquaculture, 241: 667-687.
  20. Ponurovsky, S.K and Yakovlev Y.M. (1992) The reproductive biology of the Japanese littleneck, Tapes philippinarum (A. Adams and Reeve, 1850) (Bivalvia: Veneridae). Journal of Shellfish Research, 11: 265-277.
  21. Toba, M., Miyama, Y., (1991) Gonadal development and spawning induction in artificially conditioned Manila clams Ruditapes philippinarum. Nippon Suisan Gakkaku, 57: 1269-1275 (In Japanese with English abstract). https://doi.org/10.2331/suisan.57.1269
  22. Uddin, M.J., Yang, H.S., Choi, S.K. (2010) Seasonal changes on Perkinsus olseni infection and gametogenesis in Manila clam Ruditapes philippinarum, from Seonjaedo island on Incheon, off the west coast of Korea. journal of World Aquaculture, 41: 93-101. https://doi.org/10.1111/j.1749-7345.2009.00337.x
  23. Yap, G.W. (1977) Population biology of the Japanese little-neck clam, Tapes philippinarum, in Kaneohe bay, Oahu, Hawaiian Islands. Pacific Science, 31: 223-244.
  24. Zhang, G and Yan, X. (2006) A new three-phase culture method for Manila clam, Ruditapes philippinarum, farming in northern China. Aquaculture, 258: 452-461. https://doi.org/10.1016/j.aquaculture.2006.04.046

Cited by

  1. First observation on the early embryonic and larval development of spiny oyster Saccostrea kegaki Torigoe & Inaba, 1981 (Bivalvial: Ostreoida) using scanning electron microscope on the north coast of Jeju, Korea vol.29, pp.2, 2013, https://doi.org/10.9710/kjm.2013.29.2.97
  2. 고창 갯벌에서 중국산 및 충남산 바지락의 성장, 비만도 및 폐사 특성 비교 vol.32, pp.3, 2012, https://doi.org/10.9710/kjm.2016.32.3.175