• Title/Summary/Keyword: Large dynamic range

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Cytochrome c Peroxidase: A Model Heme Protein

  • Erman, James E.;Vitello, Lidia B.
    • BMB Reports
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    • v.31 no.4
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    • pp.307-327
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    • 1998
  • Cytochrome c peroxidase (CcP) is a yeast mitochondrial enzyme which catalyzes the reduction of hydrogen peroxide to water using two equivalents of ferrocytochrome c. The CcP/cytochrome c system has many features which make it a very useful model for detailed investigation of heme protein structure/function relationships including activation of hydrogen peroxide, protein-protein interactions, and long-range electron transfer. Both CcP and cytochrome c are single heme, single subunit proteins of modest size. High-resolution crystallographic structures of both proteins, of one-to-one complexes of the two proteins, and a number of active-site mutants are available. Site-directed mutagenesis studies indicate that the distal histidine in CcP is primarily responsible for rapid utilization of hydrogen peroxide implying significantly different properties of the distal histidine in the peroxidases compared to the globins. CcP and cytochrome c bind to form a dynamic one-to-one complex. The binding is largely electrostatic in nature with a small, unfavorable enthalpy of binding and a large positive entropy change upon complex formation. The cytochrome c-binding site on CcP has been mapped in solution by measuring the binding affinities between cytochrome c and a number of CcP surface mutations. The binding site for cytochrome c in solution is consistent with the crystallographic structure of the one-to-one complex. Evidence for the involvement of a second, low-affinity cytochrome c-binding site on CcP in long-range electron transfer between the two proteins is reviewed.

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Fabrication of Microstructure Array using the Projection Microstereolithography System (전사방식 마이크로광조형을 이용한 배열 형태 미세 구조물 가공)

  • Choi, Jae-Won;Ha, Young-Myoung;Lee, Seok-Hee
    • Journal of the Korean Society for Precision Engineering
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    • v.24 no.8 s.197
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    • pp.138-143
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    • 2007
  • Microstereolithography technology is similar to the conventional stereolithography process and enables to fabricate a complex 3D microstructure. This is divided into scanning and projection type according to aiming at precision and fabrication speed. The scanning MSL fabricates each layer using position control of laser spot on the resin surface, whereas the projection MSL fabricates one layer with one exposure using a mask. In the projection MSL, DMD used to generate dynamic pattern consists of $1024{\times}768$ micromirrors which have $13.68{\mu}m$ per side. The fabrication range and resolution are determined by the field of view of the DMD and the magnification of the projection lens. If using the projection lens with high power, very fine microstructures can be fabricated. In this paper, the projection MSL system adapted to a large surface for array-type fabrication is presented. This system covers the meso range, which is defined as the intermediate range between micro and macro, with a resolution of a few ${\mu}m$. The fabrication of array-type microstructures has been demonstrated to verify the performance of implemented system.

Large-Scale Structure of Leading-Edge Separation Bbubble with Local Forcing (국소교란이 가해지는 박리기포의 대형구조)

  • 김유익;성형진
    • Transactions of the Korean Society of Mechanical Engineers
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    • v.19 no.4
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    • pp.1134-1147
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    • 1995
  • POD (proper orthogonal decomposition) is applied to turbulent leading-edge separation bubble to extract coherent structures. A two-dimensional leading-edge separation bubble is simulated by discrete-vortex method, where a time-dependent source forcing is incorporated. Based on the wealth of numerical data, POD is applied in a range of the forcing amplitude ( $A_{o}$ = 0, 0.5, 1.0 and 1.5) and forcing frequency (0 .leq. $f_{F}$H/ $U_{\infty}$.leq. 0.3). It is demonstrated that the structures of POD have noticeable changes with local forcings. In an effort to investigate the mechanism of decreasing reattachment length, dynamic behaviors of the expansion coefficients and contributions of the eigenfunctions of POD are scrutinized. As the forcing amplitude increases, the large-scale vortex structures are formed near the forcing amplitude increases, the large-scale vortex structures are formed near the separation point and the flow structures become more organized and more regular, accompanying with the reduction of reattachment length. By further inverstigation of POD global entropy, it is seen that the reattachment length is closely linked to the degree of organization of the flow structures.es.s.

Predictive Study of Rubber Friction Considering Large Deformation Contact (대변형 접촉을 고려한 고무 마찰 예측 연구)

  • Nam, Seungkuk
    • Tribology and Lubricants
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    • v.34 no.1
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    • pp.1-8
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    • 2018
  • This paper presents the analysis of friction master curves for a sliding elastomer on rough granite. The hysteresis friction is calculated using an analytical model that considers the energy spent during the local deformation of the rubber due to surface asperities. The adhesion friction is also considered for dry friction prediction. The viscoelastic modulus of the rubber compound and the large-strain effective modulus are obtained from dynamic mechanical analysis (DMA). We accurately demonstrate the large strain of rubber that contacts with road substrate using the GW theory. We found that the rubber block deforms approximately to 40% strain. In addition, the viscoelastic master curve considering nonlinearity (at 40% strain) is derived based on the above finding. As viscoelasticity strongly depends on temperature, it can be assumed that the influence of velocity on friction is connected to the viscoelastic shift factors gained from DMA using the time-temperature superposition. In this study, we apply these shift factors to measure friction on dry granite over a velocity range for various temperatures. The measurements are compared to simulated hysteresis and adhesion friction using the Kluppel friction theory. Although friction results in the low-speed band match well with the simulation results, there are differences in the predicted and experimental results as the velocity increases. Thus, additional research is required for a more precise explanation of the viscoelastic material properties for better prediction of rubber friction characteristics.

Regional Transformation in 'Myeon' Administrative District adjacent to Urban Area (도시주변 면단위 행정구역의 지역 변화 -전라북도 조촌면을 사례로-)

  • Cho, Sung-Wook
    • Journal of the Korean association of regional geographers
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    • v.12 no.1
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    • pp.59-71
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    • 2006
  • The purpose of this study is to explain the regional transformation in the lowest level administration district(Myeon). The major factor of regional transformation is the change of geographical range, identity of place name, the formation and change of regional center. Jo-Chon Myeon as a sample study region is located in near Jeon-Ju city. The large and dynamic city in neighbor is strong influenced to the change of geographical range. But the place name has Identity in spite of the regional change. The formation of new regional center is influenced by new road, rail road and station, japanese large farm, administration office in this district.

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Numerical simulation of Hydrodynamics and water properties in the Yellow Sea. I. Climatological inter-annual variability

  • Kim, Chang-S.;Lim, Hak-Soo;Yoon, Jong-Joo;Chu, Peter-C.
    • Journal of the korean society of oceanography
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    • v.39 no.1
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    • pp.72-95
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    • 2004
  • The Yellow Sea is characterized by relatively shallow water depth, varying range of tidal action and very complex coastal geometry such as islands, bays, peninsulas, tidal flats, shoals etc. The dynamic system is controlled by tides, regional winds, river discharge, and interaction with the Kuroshio. The circulation, water mass properties and their variability in the Yellow Sea are very complicated and still far from clear understanding. In this study, an effort to improve our understanding the dynamic feature of the Yellow Sea system was conducted using numerical simulation with the ROMS model, applying climatologic forcing such as winds, heat flux and fresh water precipitation. The inter-annual variability of general circulation and thermohaline structure throughout the year has been obtained, which has been compared with observational data sets. The simulated horizontal distribution and vertical cross-sectional structures of temperature and salinity show a good agreement with the observational data indicating significantly the water masses such as Yellow Sea Warm Water, Yellow Sea Bottom Cold Water, Changjiang River Diluted Water and other sporadically observed coastal waters around the Yellow Sea. The tidal effects on circulation and dynamic features such as coastal tidal fronts and coastal mixing are predominant in the Yellow Sea. Hence the tidal effects on those dynamic features are dealt in the accompanying paper (Kim et at., 2004). The ROMS model adopts curvilinear grid with horizontal resolution of 35 km and 20 vertical grid spacing confirming to relatively realistic bottom topography. The model was initialized with the LEVITUS climatologic data and forced by the monthly mean air-sea fluxes of momentum, heat and fresh water derived from COADS. On the open boundaries, climatological temperature and salinity are nudged every 20 days for data assimilation to stabilize the modeling implementation. This study demonstrates a Yellow Sea version of Atlantic Basin experiment conducted by Haidvogel et al. (2000) experiment that the ROMS simulates the dynamic variability of temperature, salinity, and velocity fields in the ocean. However the present study has been improved to deal with the large river system, open boundary nudging process and further with combination of the tidal forcing that is a significant feature in the Yellow Sea.

Analysis of Rheological Properties of Cement Paste with Binder Type and Composition Ratio (결합재 타입 및 구성비 변화에 따른 시멘트 페이스트의 레올로지 특성 분석)

  • Jeon, Sung IL;Nam, Jeong Hee;Lee, Moon Sup;Nho, Jae Myun
    • International Journal of Highway Engineering
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    • v.19 no.5
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    • pp.77-88
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    • 2017
  • PURPOSES : It is necessary to clarify the rheological properties of cement paste as a basic research in the development of mechanistic concrete mix design. The rheological properties of cement paste with different binder types, mix propositions, and with/without high range water reducers have been analyzed. METHODS : In this study, ordinary Portland cement, fly-ash, blast furnace slag, silica fume, and limestone powder were used as binders. The range of water-binder ratio was 0.3-0.5, and a total of 30 different mixes have been tested. The slump flow test, V-funnel test, and Dynamic Shear Rheometer (DSR) test were performed to analyze the rheological properties of cement paste. RESULTS : As a result of the slump flow test, it was found that the composition ratio of the binder contents greatly affected the paste flow when the high range water reducers were added. The results of V-funnel test showed that when the water-binder ratio was decreased without high range water reducers, the binder composition ratio had a large effect on the passing time of the V-funnel tester, but with high range water reducers the impact of the binder composition ratio was decreased. The slump flow and V-funnel have a certain relationship with the rheological factors (yield stress and plastic viscosity), but the correlation was not significant. Finally, we proposed the M-value considering the density and specific surface area of the binder. The correlation between rheological factors and M-value were better demonstrated than experimental values, but there is still a limit to predict the rheological factor in general mix design. CONCLUSIONS :In this study, the rheological properties of cement paste were analyzed. The binder type, composition ratio of binder, and with/without high range water reducers have combined to provide the complex effects on the rheological properties of cement paste. The correlation between the proposed M-value and rheological factor was found to be better than experimental results, but needs to be improved in the future.

Dynamic Deformation Behavior of Zr-Based Bulk Amorphous Alloy after Annealing Treatments (벌크형 비정질 Zr계 합금의 결정화 열처리에 따른 동적변형 거동)

  • Chang J. J;Lee B. J;Hwang J. I;Park I. M;Cho K. M;Cho Y. R
    • Korean Journal of Materials Research
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    • v.14 no.3
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    • pp.181-185
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    • 2004
  • The mechanical properties of a bulk amorphous alloy ($Zr_{41.2}$ $Ti_{13.8}$ /$Cu_{10}$ $Ni_{10}$ $Be_{22.5}$ /at.%) before and after an annealing treatment were investigated. For the bulk amorphous alloy, the compressive strength was about 2.0 GPa, irrespective of the strain rates in the range of $10^{-4}$ to $10^3$$ sec^{-1}$ . Fine-sized nanocrystalline particles (10~100 nm) were precipitated homogeneously in the bulk amorphous matrix after the annealing treatments. Compared to the bulk amorphous materials, these composite materials, composed of the nanocrystalline phases and a bulk amorphous matrix had much different mechanical properties. The strength and strain of coposite materials measured by a compressive test showed a peak-maximum values at 7 vol.% of the nanocrystalline phases. The values in higher volume fraction of the crystalline phases in the amorphous matrix were decreased, as measured by both quasi-static and high strain rate. The decrease in fracture strength is due to presence of the dispersed large-crystalline phases in the amorphous matrix.

A stereo matching algorithm in pixel-based disparity space image (화소기반 변이공간영상에서의 스테레오 정합)

  • 김철환;이호근;하영호
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.29 no.6C
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    • pp.848-856
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    • 2004
  • In this paper, a fast stereo matching algorithm based on pixel-wise matching strategy, which can get a stable and accurate disparity map, is proposed. Since a stereo image pair has small differences each other and the differences between left and right images are just caused by horizontal shifts with some order, the matching using a large window will not be needed within a given search range. However, disparity results of conventional pixel-based matching methods are somewhat unstable and wrinkled, the principal direction of disparities is checked by the accumulated cost along a path on array with the dynamic programming method. Experimental results showed that the proposed method could remove almost all disparity noise and set a good quality disparity map in very short time.

Evaluation of Fracture Toughness of Pressure Vessel Steel Using Charpy Impact Test Specimens (Charpy 충격시편을 이용한 압력용기 재료의 파괴인성 측정)

  • Han, Dae-June;Park, Sun-Pil
    • Nuclear Engineering and Technology
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    • v.19 no.1
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    • pp.1-9
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    • 1987
  • The fracture toughness of SA 533 Grade B Class 1 steel has been studied with the Charpy impact test specimens in a range of temperature between -4$0^{\circ}C$ and 288$^{\circ}C$. The dynamic fracture toughness is measured by the instrumented precracked Charpy impact test while the static fracture toughness is by the 3-point bend test based on the unloading compliance method. The results are compared with the data obtained from the large specimens. It is known through the studies that temperature dependence of the appropriate (a low bound) value of the fracture toughness can be estimated by taking the static fracture toughness above the transition temperature and the dynamic fracture toughness below the temperature and it is also shown that the tests are satisfied with the requirements of ASTM E 813 when the side-groove is more than 14%.

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