• Journal of Korean Society on Water Environment
• /
• v.27 no.5
• /
• pp.670-679
• /
• 2011

Chlorophyll-a is a major water quality indicator for an algal bloom in streams and lakes. The purpose of the study is to estimate chlorophyll-a concentration in tributaries of the Seonakdonggang by an artificial neural network (ANN). As the tributaries are ungauged streams, a watershed runoff and quality model was used to simulate water quality parameters. The tributary watersheds include urban area and thus Storm Water Management Model (SWMM) was used to simulate TN, TP, BOD, COD, and SS. SWMM, however, can not simulate chlorophyll-a. The chlorophyll-a series data from the tributaries were estimated by the ANN and the simulation results of water quality parameters using SWMM. An assumption used is as follows: the relation between water quality parameters and chlorophyll-a in the tributaries of the Seonakdonggang would be similar to that in the mainstream of the Seonakdonggang. On the assumption, the measurement data of water quality and chlorophyll-a in the mainstream of the Seonakdonggang were used as the learning data of the ANN. Through the sensitivity analysis, the learning data combination of water quality parameters was determined. Finally, chlorophyll-a series were estimated for tributaries of the Seonakdonggang by the ANN and TN, TP, BOD, COD, and temperature data from those streams. The relative errors between the estimated and measured chlorophyll-a were approximately 40 ~ 50%. Though the errors are somewhat large, the estimation process for chlorophyll-a may be useful in ungauged streams.

• ALGAE
• /
• v.38 no.1
• /
• pp.39-55
• /
• 2023

Exploring mixotrophy of dinoflagellate species is critical to understanding red-tide dynamics and dinoflagellate evolution. Some species in the dinoflagellate genus Karenia have caused harmful algal blooms. Among 10 Karenia species, the mixotrophic ability of only two species, Karenia mikimotoi and Karenia brevis, has been investigated. These species have been revealed to be mixotrophic; however, the mixotrophy of the other species should be explored. Moreover, although K. mikimotoi was previously known to be mixotrophic, only a few potential prey species have been tested. We explored the mixotrophic ability of Karenia bicuneiformis, Karenia papilionacea, and Karenia selliformis and the prey spectrum of K. mikimotoi by incubating them with 16 potential prey species, including a cyanobacterium, diatom, prymnesiophyte, prasinophyte, raphidophyte, cryptophytes, and dinoflagellates. Cells of K. bicuneiformis, K. papilionacea, and K. selliformis did not feed on any tested potential prey species, indicating a lack of mixotrophy. The present study newly discovered that K. mikimotoi was able to feed on the common cryptophyte Teleaulax amphioxeia. The phylogenetic tree based on the large subunit ribosomal DNA showed that the mixotrophic species K. mikimotoi and K. brevis belonged to the same clade, but K. bicuneiformis, K. papilionacea, and K. selliformis were divided into different clades. Therefore, the presence or lack of a mixotrophic ability in this genus may be partially related to genetic characterizations. The results of this study suggest that Karenia species are not all mixotrophic, varying from the results of previous studies.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.57 no.4
• /
• pp.389-396
• /
• 2024

Reactive oxygen species (ROS) generated by harmful algal blooms (HABs) exert detrimental effects on aquaculture systems. Fish gill cells deteriorate upon exposure to HABs, suggesting that internally generated ROS in HABs influences the external environment. Therefore, we investigated the internal and external changes in ROS concentrations during growth using fluorescence staining of four representative HABs: Alexandrium affine, Chattonella marina, Karenia mikimotoi, and Margalefidinium polykrikoides. The concentrations of H₂O₂ and O₂^- produced by A. affine were low; H₂O₂ from M. polykrikoides was primarily detected internally throughout the experiments, and O₂^- was not detected. High H₂O₂ and O₂^- concentrations were observed in K. mikimotoi during the death phase, with weak external O₂^- concentrations. Regarding C. marina, which produces large amounts of ROS, H₂O₂ was observed internally during the exponential phase, whereas weak O₂^- concentrations were measured externally in the stationary phases. Collectively, our results highlight that ROS concentrations and internal/external distributions are functions of HABs and growth stage. These differences indicate the potential allelopathic mechanisms of proliferating HABs and suggest a possible impact of ROS on aquaculture organisms.

• Korean Journal of Ecology and Environment
• /
• v.36 no.4 s.105
• /
• pp.467-472
• /
• 2003

In this study, an algal fence was developed and applied to reduce the input of algal scum into the water intake facility. The effectiveness of vertical algal fences(overlapped three types of meshes, (312 ${\mu}m$ ${\times}$ 375 ${\mu}m$, 390 ${\mu}m$ ${\times}$ 450 ${\mu}m$, and 0,7 cm ${\times}$ 1cm; vertical depth, 1.5 m; length of fence, about 120 m)) was experimentally tested at a water intake facility (Mulgum, lower Nakdong River). The application of the fence resulted in the statistically significant difference of algal biomass between inside and outside of the fence. According to ANOVA test, chi. a concentration in Day-1showed large difference at each depth of 0, 1, 2 m (0.001> p at each depth, n = 16 respectively). Especially large difference was observed at 0 and 1 m depth. However, the fence was only effective for a short period and its efficiency declined by Day-5after the installation. When better maintenance options for the fence are prepared, e.g. mechanical installation and periodic backwashing of the fence, the performance of algal fence may be sustained. In addition, reliable models for bloom prediction are required to provide an advanced indication of the optimal timing for the installation so that effective operation would be achieved.

• Korean Journal of Remote Sensing
• /
• v.36 no.2_2
• /
• pp.237-247
• /
• 2020

Here, we generated maps of primary production in the coastal waters of the East Sea using sea surface chlorophyll-a concentrations (CHL), photosynthetically available radiation (PAR), euphotic depth induced by GOCI along with sea surface temperature (SST) from satellites of foreign countries as input parameters, and carried out a sensitivity analysis for each parameters. On 25th of July in 2013 when a wide cold waters appeared and on 13th of August in 2013 when a big harmful algal bloom existed in the study area, it shows high productivities with averages 1,012 and 1,945 mg C m^-2 d^-1, respectively. On August 25, 2013, when the cold waters and red tide retreated, it showed an average of 778 m^-2 d^-1, similar to the results of the previous analysis. As a result of the sensitivity analysis, PAR did not significantly affect the results of the primary production, but the euphotic depth and CHL showed aboveaverage sensitivity. In particular, SST had a large influence to the results, thus we could imply that an error in SST could lead to a large error in the primary production. This study showed that GOCI data was available for primary production study, and the accuracy of input parameters might be improved by applying GOCI, which can acquire images 8 times a day, making it more accurate than foreign polar orbit satellites and consequently, it is possible to estimate highly accurately primary production.

• Journal of Korean Society on Water Environment
• /
• v.30 no.5
• /
• pp.539-548
• /
• 2014

This study was carried out to investigate algal community structures and their correlations with environmental factors on five weir areas in the Nakdong River, South Korea. Water qualities, hydrodynamics, meteorological conditions and algal species compositions were observed in studied sites from May 2010 to Dec. 2013. Results showed that average total phosphorus concentration of 2013 was decreased by 52.4% in comparing with that from 2010 to 2011. Chlorophyll.a concentrations were positive significant with water temperature, pH, total phosphorus and total nitrogen, but is not significant with turbidity and suspended solids. Seasonal successions of algae were observed that Stephanodiscus sp. was dominant species with 65.3% of dominant frequency in studied site. Large algal biomass of the low temperature-adapted diatoms were observed during temperature range of $4{\sim}9^{\circ}C$, but large cyanobacterial biomass mainly during high temperature period ranged from $22^{\circ}C$ to $32^{\circ}C$. Microcystis sp. dominated during high water temperature in summer. The yearly correlations of algal biomass with accumulated solar radiations were not significant but seasonal correlations of summer from June to August were significant with correlation coefficient 0.33 (p<0.05). There were not significant correlations between turbidities and algal biomass. Turbidity and suspended solids concentrations were not significant correlation with algal biomass. According to the results, algal communities had strong correlation with water temperature and had partially correlation with solar radiation. For an effective management of algal blooms, water managers should survey with more long-term monitoring of various environmental factors and algal communities.

• Journal of the Korean Society of Marine Environment & Safety
• /
• v.24 no.7
• /
• pp.967-972
• /
• 2018

The genus Chattonella belonging to the class raphidophyceae, is a harmful algal bloom species. Recently, its occurrence has been increasing and expanding along the Korean coast. Species identification of the genus Chattonella only by morphological observation is difficult due to the lack of rigid cell walls. In this study, the morphological characteristics and genetic affinity of Chattonella sp. isolated from Deungnyang Bay in 2017 were examined. We carried out single-cell isolation from field samples then sequenced three different areas using the single-cell PCR method: 1) parts of ribosomal operon, the large subunit (LSU) of the rDNA, 2) the chloroplast-encoded subunit psaA of Photosystem I, and 3) rbcL encoding the large subunit of the Rubisco gene. The cells were morphologically very similar to the general genus Chattonella ($74.0{\pm}10.1{\mu}m$ in length, $33.1{\pm}3.6{\mu}m$ in width). The three partial gene sequences were insufficient to justify distinction at the species rank. However, they clustered at 99-100 % sequence similarity with C. marina, C. marina var. antiqua and C. marina var. ovata.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
• /
• v.7 no.3
• /
• pp.140-147
• /
• 2002

We investigated the temporal variations in the heterotrophic dinoflagellates (hereafter HTD), tintinnid ciliates(TC), and naked ciliates(NC) from August to November 1999 in the coastal waters off the southern Saemankeum areas where a huge red tide dominated by Cochlodinium polykrikoides/Gymnodinium impudicum was first observed in 1998. We took water samples from 2-5 depths of 4 stations in each of the 5 cruises and then measured the species composition and abundances of HTD, TC, and NC The maximum species numbers and densities of HTD, TC, and NC(11, 12, and 10 cells $m\ell$$^{-1}$ , respectively) were observed when the density of diatoms was highest (August 10), while the lowest values (1.0, 0.5, and 2.4 cells $m\ell$$^{-1}$ , respectively) were found when the red tide dominated by C. polykrikoides/G. impudicum took placed (October 18). On August 10 and November 11 when diatoms dominated the abundance of phytoplankton, the correlation coefficients between TC, NC and diatoms were relatively high. However, On September 16 and October 18 when autotrophic+mixotrophic dinoflagellates(ATD+MTD) were abundant, the correlation coefficients between HTD and ATD+MTD were relatively high. The large HTD Noctiluca scintillans was the most dominant heterotrophic protists during the C. polykrikoides/G. impudicum red tide on October 18. N. scintillans has been known to feed on the prey cells when the swimming speeds of C. polykrikikoides/G. impudicum markedly reduced at the decline stage of the red tide. Therefore, N. scintillans could be effective grazers on C. polykrikoides/G. impudicum. The maximum densities of HTD, TC, and NC in the study area were fairly lower than those obtained in the waters off Kohung-Yeosu areas in the summer-fall, 1997. The results of the present study provide the basis of understanding predator-prey relationships between dominant phytoplankton and heterotrophic protists and the roles of the protist grazers in bloom dynamics in the waters off the western Korea.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
• /
• v.7 no.3
• /
• pp.181-194
• /
• 2002

Parasitism is a one-sided relationship between two organisms in which one benefits at the expense of the other. Parasitic dinoflagellates, particularly species of Amoebophrya, have long been thought to be a potential biological agent for controlling harmful algal bloom(HAB). Amoebophrya infections have been reported for over 40 species representing more than 24 dinoflagellate genera including a few toxic species. Parasitic dinoflagellates Amoebophrya spp. have a relatively simple life cycle consisting of an infective dispersal stage (dinospore), an intracellular growth stage(trophont), and an extracellular reproductive stage(vermiform). Biology of dinospores such as infectivity, survival, and ability to successfully infect host cells differs among dinoflagellate host-parasite systems. There are growing reports that Amoebophrya spp.(previously, collectively known as Amoebophrya ceratii) exhibit the strong host specificity and would be a species complex composed of several host-specific taxa, based on the marked differences in host-parasite biology, cross infection, and molecular genetic data. Dinoflagellates become reproductively incompetent and are eventually killed by the parasite once infected. During the infection cycle of the parasite, the infected host exhibits ecophysiologically different patterns from those of uninfected host in various ways. Photosynthetic performance in autotrophic dinoflagellates can be significantly altered following infection by parasitic dinoflagellate Amoebophrya, with the magnitude of the effects over the infection cycle of the parasite depending on the site of infection. Parasitism by the parasitic dinoflagellate Amoebophrya could have significant impacts on host behavior such as diel vertical migration. Parasitic dinoflagellates may not only stimulate rapid cycling of dissolved organic materials and/or trace metals but also would repackage the relatively large sized host biomass into a number of smaller dinospores, thereby leading to better retention of host's material and energy within the microbial loop. To better understand the roles of parasites in plankton ecology and harmful algal dynamics, further research on a variety of dinoflagellate host-parasite systems is needed.

• Korean Journal of Ecology and Environment
• /
• v.41 no.spc
• /
• pp.68-76
• /
• 2008

This study examined the inhibition effects of a freshwater bivalve (Unio douglasiae) and a submerged plant (Potamogeton crispus) on the cyanobacterial bloom (Oscillatoria sp.). The experiment were conducted in aquarium $(50cm{\times}65cm{\times}120cm)$ with lake sediments in the bottom of the aquarium in 10 cm thick. Before the experiments, artificial cyanobacterial bloom was induced with the addition of lake sediment and CB medium. Total 12 transparent acrylic cylinders (${\Phi}19cm$, height 40 cm) were placed in the aquarium, and within which bivalves and plants were placed in various conditions such as the control (C), plant addition (P:5 stems), mussel addition (U:2 individuals), and both mussel and plant addition (PU: the same quantity as used in each treatment). The experiment was conducted in triplicate during 7 days. pH, dissolved oxygen (DO), electric conductivity (EC), salinity, cyanobacterial cell density, chlorophyll-${\alpha}$ concentration, and mussel filtering rate were monitored daily. At the end of the experiment, total phosphorus (TP), total nitrogen (TN), and plant height and weight were measured. Overall, a large degree of cyanobacterial growth inhibition appeared in both P and U treatments, and the effect was highest in the U treatment, followed by P and PU. The combined treatment of both U and P did not show any synergic effects compared to the effect in separated treatment. In all enclosures of the treatments chlorophyll-${alpha}$ (Chl-${alpha}$) concentration decreased until 36 hours after the additions of the plants and mussels. In contrast, Chl-${alpha}$ concentrations increased in PU enclosures after 36 hours. The same trend was shown in the cell density of Oscillatoria. pH and DO gradually decreased until 120 and 144 hours, respectively, in the P and PU enclosures. TP concentration increased in the mussel enclosures (U and PU), while TN concentration largely decreased in the plant enclosures (P and PU). Our results suggest that applied bivalve (Unio) and submerged plant (Potamogeton) seemed to have a potential effect on the growth inhibition of cyanobacteria, but their combined application may have an antagonistic effect to diminish the degree of the inhibition.