• Journal of the Korean Society of Tobacco Science
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• v.3 no.2
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• pp.77-81
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• 1981

A flew spectroscopic method was developed to determine microquantities of nicotine with cyanogen chloride and pyrazolon. Absorption maximum of tile derivative solution appeared at 520nm. The color formed in the proposed method was stable for at least 30 min. at pH 7.5, and followed the Lambert-Beer's law between tile limits of 1 and 5 micrograms of nicotine. The relative errors in burley and flue-cured tobacco leaves were $\pm5%$ in comparison with the Griffith method.

• Journal of the Korean Society of Tobacco Science
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• v.2 no.2
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• pp.38-43
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• 1980

The spectrophotometric determination oi reducing sugar in the tobacco leaves was carried out by employing blue tetrazolium chloride as a color developing reagent. The absorption maximum of formazan dye which is the product of the reaction between blue tetrazolium chloride and fructose was shown to be $530^{nm}$. In the fructose concentration range of 0.02mg/ml-0.14mg/ml the calibration curve was well coincided to the law of Beer-Lambert. In order to take a look at the accuracy and/or recovery rate of fructose determination, the standard fructose was added to the tobacco leaves and the concentration of this standard fructose was estimated. A tittle lower concentration of the standard fructose compared with the pure one in solution was observed. However, an excellent analytical recovery was revealed under the -2% of relative error limit. When we carried out the quantitative determination of this reducing sugar by the method of visual read-out (without using the spectrophotometer), the relative error was obtained to be $\pm$10%.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2003.10a
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• pp.860-863
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• 2003

Pulse oximetry has gained wide spread clinical acceptance in the latter part of the 21st century. The principle of pulse oximetry is based on the red and infrared light absorption features and uses a light emitter with red and infrared LEDs that shines through a reasonably translucent site with good blood flow. There are two methods of sending light through the measuring site : transmission and reflectance. After the transmitted red and infrared signals pass through the measuring site and received at the photodetector, the red/infrared ratio is calculated. But, pulse of oximeters are so sensitive that they may detect pulses when pressure is too low to provide adequate tissue blood flow, that is, SpO2 may decrease due to O2 consumption by the finger of the pulsing but stagnant arterial blood at low pressure or with vasoconstriction. This project has the limitations of pulse oximetry. Therefore, this paper is focused on the resuction of motion artifact that caused by moving when someone measures with SpO2 system.

• Journal of the Korean Society of Tobacco Science
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• v.2 no.2
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• pp.14-19
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• 1980

The spectrophotometric determination of nictotine content with barbituric acid buffer solution was carry out. Absorption maximum for the proposed solution appeared at 505 nm and the absorbance remained stable for 15 minute at pH 4.2. Lambert-Beer law was proved to be applicable in the range of nicotine concentration of $7.5\mu{g}/ml$~$25\mu{g}/ml$. According to the analysis of nicotine contents in Burley and Flue-cured tobacco leaves by this method, the relative deviation was obtained to be 2.8% in comparision with the Griffith method.

• Proceedings of the Korean Vacuum Society Conference
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• 2015.08a
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• pp.155.1-155.1
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• 2015

Nowadays, Plasma has been used in biological, medical such as wound healing, plant grow, killing cancer. When plasma generated, UV light and ROS(Reactive oxygen species), RNS(Reactive nitrogen species) can generated and those things effect to biological material. So we made simple plasma device using needle type of electrode and generated plasma. We used three kinds of gas and measured applied voltage and current. Also we observed optical emission spectrum. Using deuterium ramp, we can observed absorption spectrum and calculated radical density by lambert-beer's law. It is around ~1016cm3. And we can see the time-resolved absorption spectrum from monochromator, PMT(photo multiply tube), IV-converter, oscilloscope.

• YAKHAK HOEJI
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• v.31 no.2
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• pp.45-51
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• 1987

Methyl orange(MO) and cetyltrimethylammonium bromide(CTA) were mixed and added to 10ml of the buffer solution and then the solution was shaken for 5 minutes. The maximum absorption wavelength of the reaction product was 419nm. Dichloromethane was the best extracting solvent among the several organic solvents and the most suitable pH range was 2~8. When the CTA-MO calibration curve was made in the best experimental condition, the Lambert-Beer's law was obeyed in the range of CTA concentration of $1.5\times$$10^{-5}$~1.0$\times$$10^{-4}M$ by UV spectrophotometer. This method was possible to determine quaternary ammonium salts in the pharmaceutical preparations.

• Journal of Pharmaceutical Investigation
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• v.1 no.1
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• pp.47-52
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• 1971

Application of the spectrophotometer to the analysis of 17 ${\alpha}-ethinyl$ estradiol and $17{\alpha}-ethinyl-19-nortestosterone$ acetate mixture in oral contraceptive has been accomplished. It is used Beckman Du Spectrophotometer as a apparatus. The petroleum ether extract of ethinyl estradiol is determined at $535\;m{\mu}$ and the chloroform extract of norethindrone acetate is determined at $380\;m{\mu}$ respectively. This analytical method is formed Lambert Beer's law. This method can be used to the analysis of ethinyl estradiol aid norethindrone acetate mixture in commercial dosage form of routine assay.

• YAKHAK HOEJI
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• v.35 no.1
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• pp.15-21
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• 1991

Quaternary amines which are widely used as medicines are nitrogen compounds. Metanil yellow(MY) and benzalkonium chloride(BKC) were mixed and added to 10ml of the buffer solution and then the solution was shaken for 5 minutes. The maximum absorption wavelength of the reaction product was 402 nm. Dichloromethane was the best extracting solvent among the several organic solvents and the most suitable pH range was 2~8. When the BKC-MY calibration curve was made in the best experimental condition, the Lambert-Beer's law was obeyed in the range of BKC concentration of 2$\times$$10^{-6}$~9$\times$$10^{-6}$M by UV spectrophotometer. This method was possible to determine quaternary ammonium salts in the pharmaceutical preparation.

• YAKHAK HOEJI
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• v.38 no.4
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• pp.366-371
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• 1994

To improve the identification power of TLC/HPTLC the in situ reflectance spectra obtained directly from plates with commercial scanner are used. The spectrum normalization should be carried out prior to comparing and searching the spectra from library for the identification of compounds. Because the reflectance does not obey the Lambert-Beer's law, there arise some problems in normalization. These problems could be solved to some extent by normalizing the spectra with regression methods. The spectra are manipulated with the regression function of a curve obtained from the correlation plot. When the parabola was used as the manipulating function, the spectra were identified with the accuracy of 97% and this result was better than that of conventionally used the point and area normalization method.

• Bulletin of the Korean Space Science Society
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• 1993.10a
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• pp.11-11
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• 1993

The Korean sounding rockets(KSR) 1, 2 equigped with the UV optical detectors have been launched at An-heung, Chungchongnam-do, of June 4 and September 1, 1993,respectively. The UV detector is used to measure the attenuation of solar UV radiation for various frequency bards in the stratospgere. We obtained the profile of the ozone number density within the altitulde of 15 - 30 km by applying the Beer-Lambert law. It is found that the maHimum of the ozone distribution occurs near 2sht which is quite consistent with the mean value in the mid-latituderegions. We will also compare our results froiu KSR 1,2 with the other observational data from the Dobson speceo meter at Yonsei Univ. and the LIDAa which were performed simultaneously with the sounding rocket experiments.