• Journal of Animal Science and Technology
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• v.46 no.4
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• pp.625-634
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• 2004

The objective of this experiment is to study the possibility of lactate dehydrogenase(LDH) enzyme to prevent lactate accumulation in the rumen, For understanding capacity of bacterial LDH in rumen environments, this study was conducted to explore the effects of temperature, pH, VFAs and metal ions on Lactobacillus sp. FFy111-1's LDH activity, and the LDH activation in rumen fluid accumulated lactate. The optimum pH and temperature of LDH were pH 7.5 and 40$^{\circ}C$, respectively. The LDH activity had a good thennostability at range from 30 to 50$^{\circ}C$. The highest pH stability of the enzyme was at ranges from pH 7.0 to 8.0 and the enzyme activities showed above 64% level of non-treated one at pH 6.0 and 6.5. The LDH was inactivated by VFAs treatments but was enhanced by metal ion treatments without NaCl and $CuSO_4$ Especially, the LDH activity was increased to 127% and 124% of its original activity by 2 mM of $BaCl_2$ and $MnSO_4$, addition, respectively. When the acidic rumen fluid was treated by LDH enzyme of Lactobacillus sp. FFy111-1, the lactate concentration in the rumen fluid was lower compared with non-treated rumen fluid(P<0.05). This lactate reduction was resulted from an action of LDH. It was proved by result of purified D,L-LDH addition that showed the lowest lactate concentration among the treatments(P<0.05). Although further investigation of microbial LDH and ruminal lactate is needed, these findings suggest that the bacterial LDH has the potential capability to decrease the lactate accumulated in an acidic rumen fluid. Also, screening of super LDH producing bacteria and technical development for improving enzyme activity in rumen environment are essential keys for practical application.

• Journal of the Korean Society of Food Science and Nutrition
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• v.27 no.5
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• pp.869-874
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• 1998

The effect of chitosanoligosaccharide(CTO) on kimchi fermentation was investigated to see the optimal CTO concentration adding into Kimchi. Lactobacillus plantarum and Leuconostoc mesenteroides were cultured in flasks under the condition of various CTO concentrations. In the case of Lactobacillus plantarium, the growth was inhibited in the degree with 52, 79 and 100% at the concentration of 0.005, 0.007, 0.05% CTO after 14 hours culture, respectively. The growth of Leuconostoc mesenteroides was significantly inhibited in the degree with 7,33 and 90% at the concentration of 0.002, 0.003 and 0.004% CTO after the culture, respectively. Kimchi was formulated with variious CTO concentrations(0.005~0.2%) and fermented at 2$0^{\circ}C$ during 12 days. The fermentation periods were increased 2~6 times more than that of control(0% CTO). Also, off-flavour by adding CTO was insignificant in all the kimchi samples.

• Journal of Dairy Science and Biotechnology
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• v.40 no.1
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• pp.15-22
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• 2022

This study evaluated the levels of D(-)-lactate and L(+)-lactate, and the ratio of D(-)-lactate to total lactate (D(-)-lactate + L(+)-lactate) of 15 lactic acid bacteria (LAB) using an enzymatic method. D(-)-lactate and L(+)-lactate levels in the LAB ranged from 0.31 to 13.9 mM and 0.76 to 39.3 mM, respectively, in Bifidobacterium sp.; 1.08 to 11.7 mM and 0.69-13.0 mM in Lactobacillus sp.; 0.72 to 20.3 mM and 0.98 to 32.3 mM in Leuconostoc sp., and 33.0 mM and 39.2 mM in Pediococcus acidilacti KCCM 11747. The ratio of the range of D(-)-lactic acid to total lactic acid was 28.98%-45.76% in Bifidobacterium sp., 41.18%-61.02% in Lactobacillus sp., 29.85%-42.36% in Leuconostoc sp., and 45.71% in P. acidilacti KCCM 11747. In the future, there is a need to test for D(-)-lactate in various fermented products to which different LAB have been added and study the screening of LAB used as probiotics that produce various concentrations of D(-)-lactate.

• Asian Journal of Turfgrass Science
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• v.24 no.2
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• pp.138-144
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• 2010

This study was conducted to investigate the effect of Lactobacillus sp. and Saccharomyces sp. on turf quality, shoot and root growth of creeping betgrass in golf course by measuring turf color index, chlorophyll content, dry weight of shoot and root, T/R ratio and root length. Fertilizer treatment was designed as follows; nonfertilizer (NF), control (CF; compound fertilizer), microorganism medium(MO; CF+MO)), microorganism medium contained Fe(MO-Fe; CF+MO-Fe) and microorganisum medium contained S (MO-S; CF+MO-S). Soil properties investigated after experiment was scarcely affected by applied fertilizers in root zone of creeping bentgrass. The turf color index and chlorophyll index of MO, MO-Fe, MO-S treatment were higher than those of NF, and similar to those of CF. The turfgrass root in MO and MO-Fe treatment was longer than others. The dry weight of shoot in MO and MO-S was higher than CF and that of root in MO and MO-Fe, and dry weight of MO was increased than that of NF and CF, by 26% and 6%, respectively. AS compared with NF, T/R ratio of CF, MO, MO-Fe and MO-S was increased, and MO and MO-Fe was similar to CF, MO-S higher. Nutrient content in CF, MO, MO-Fe and MO-S was contained more than in NF, and it was higher in shoot. These was suggested that application of MO induced the development of quality and growth of creeping bentgrass by assisting root growth and nutrients uptake.

• Food Science of Animal Resources
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• v.29 no.5
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• pp.640-646
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• 2009

Lactic acid bacteria (LAB) are typical probiotic microbes that are used in various industries including fermented foods, feed additives, and pharmaceuticals. The purpose of this study was to compare the ability of isoflavone biotransformation and antioxidative activity of 17 LAB. Six strains including the Lactobacillus species exhibited a 100% hydrolysis rate for daidzein during fermentation. The content of total genistein in soymilk fermented with these strains was $872-943\;{\mu}g/g$. The DPPH (1, 1-diphenyl-2-picrylhydrazyl) radical scavenging ability of the LAB was widely variable and ranged from 23-78%. A selected strain was isolated from kimchi and the strain was identified as Lactobacillus plantarum ssp. through the API carbohydrate fermentation pattern and 16S rDNA profile. The strain exhibited excellent acid tolerance in an artificial gastric solution. L. plantarum YS712 showed high $\beta$-glucosidase activity in fermentation. The concentration of genistein and daidzein in soymilk fermented with L. plantarum YS712 increased from 3.64 to $917.3\;{\mu}g/g$ and from 58.18 to $1062.17\;{\mu}g/g$, respectively. These results demonstrate the potential of L. plantarum YS712 as a probiotic culture that can be utilized in the manufacturing of fermentation foods and dietary supplements.

• KSBB Journal
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• v.20 no.3
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• pp.228-232
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• 2005

In this study, Artemisia mongolica fischer extract was examined for its possible differential inhibitory activity against pathogenic bacteria including Staphylococcus aureus and Lactobacillus spp. isolated from women's vagina. First, seven lactobacillus spp. were selected based on their in vitro high anti-staphylococcal activity. Various samples extracted using different concentrations of organic solvents (acetone, ethanol, methanol) were examined for optimal anti-staphylococal activity. When the Artemisia extract obtained using $100\%$ solvents was added to the cell suspension at $17\%$ (vol/vol), Lactobacillus sp. KLB 224 maintained its viability for 48 hr, whereas S. aureus was completely inactivated showing differential antimicrobial activity of the extract. Using scanning electron microscopy the effect of the extract on the cell morphology was observed: S. aureus showed markedly distorted cell morphology while Lactobacillus sp. KLB 224 appeared to remain intact.

• Microbiology and Biotechnology Letters
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• v.28 no.2
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• pp.124-127
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• 2000

Beneficial bacteria, which have been used for medical purpose and for medicines for treating intestinal disorders, include strains of Bifidobacterium sp., Lactobacillus sp., Enterococcus sp., Clostridium butyricum, Lactobacillus sporogenes, Bacillus subtilis, Bacillus polyfermenticus and the like. Bacillus polyfermenticuss SCD with is commonly called as Bispan strain has been appropriately used for the treatment of long-term intestinal disorders, since the live strains in the form of active endospores can successfully reach the target intestine. In this study, the identification and characterization of Bispan strain was done using SEM observation, API 50CHB kits, isoprenoid quinone analysis, and fatty acid analysis. These results suggest that Bispan strain is very similar to Bacillus subtilis.

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 2003.10a
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• pp.201.1-201
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• 2003

생균제로서 가능성 있는 유산균의 선별을 위해 발효식품인 동치미로부터 분리된 균주들의 내산성, 내담즙성, 내췌장액성 및 유해균 억제능을 조사하였다. 특히 분리된 균종 중에서 FF-3가 인공위액과 담즙산에 대한 높은 내성을 나타내었을 뿐만 아니라, Salmonella sp. 과 Escherichia coli에 대하여 높은 항균 활성을 나타내었다. FF-3유래 박테리오신은 그람양성 및 음성 세균에서 넓은 항균 범위를 나타내었다. 본 연구에서 최종선별된 FF-3 균주를 16s rDNA sequence 동정한 결과 Lactobacillus sp.으로 확인되어 FF-3으로 명명하였다.

• Biotechnology and Bioprocess Engineering:BBE
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• v.9 no.4
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• pp.303-308
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• 2004

We isolated a novel lactic acid bacterium from a Korean traditional fermented food, soybean paste. The newly isolated strain, dubbed RKY2, grew well on glucose, sucrose, galactose, and fructose, but it could not utilize xylose, starch, or glycerol. When the partially amplified 16S rDNA sequence (772 bp) of the strain RKY2 was compared with 10 reference strains, it was found to be most similar to Lactobacillus pentosus JCM $1588^T$, with 99.74% similarity. There-fore, the strain RKY2 was renamed Lactobacillus sp. RKY2, which has been deposited in the Korean Collection for Type Cultures as KCTC 10353BP. Lactobacillus sp. RKY2 was found to be a homofermentative lactic acid bacterium, because its end-product from glucose metabolism was found to be mainly lactic acid. It could produce more than 90 g/L of lactic acid from MRS medium supplemented with 100 g/L of glucose, with 5.2 g $L^-1$ $h^-1$ of productivity and 0.95 g/g of lactic acid yield.

• Biotechnology and Bioprocess Engineering:BBE
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• v.9 no.4
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• pp.318-322
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• 2004

Lactic acid is an environmentally benign organic acid that could be used as a raw material for biodegradable plastics if it can be inexpensively produced by fermentation. Two genes (ldhL and ldhD) encoding the L-(+) and D-(-) lactate dehydrogenases (L-LDH and D-LDH) were cloned from Lactobacillus sp., RKY2, which is a lactic acid hyper-producing bacterium isolated from Kimchi. Open reading frames of ldhL for and ldhD for the L and D-LDH genes were 962 and 998 bp, respectively. Both the L(+)- and D(-)-LDH proteins showed the highest degree of homology with the L- and D-lactate dehydrogenase genes of Lactobacillus plantarum. The conserved residues in the catalytic activity and substrate binding of both LDHs were identified in both enzymes.